LOCUS       sequence-21217-e         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Barboric M, Kohoutek J, Price JP, Blazek D, Price DH, Peterlin BM
  TITLE     Interplay between 7SK snRNA and oppositely charged regions in HEXIM1
            direct the inhibition of P-TEFb.
  JOURNAL   EMBO J. 2005 Dec 21. 24(24):4291-303.
  PUBMED    16362050
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            2005 Dec 21. 24(24):4291-303."
COMMENT     Sequence Label: pcDNA3 Hexim2 (P#1897)
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             118..135
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             139..171
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(175..183,184..198)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctg gctagcgttt aaacttaagc ttaccatggg gggttctcat
      121 catcatcatc atcatggtat ggctagcatg actggtggac agcaaatggg tcgggatctg
      181 tacgacgatg acgataaggt acctatgatg gccactccga accagaccgc ctgtaatgca
      241 gagtcaccag tggccctgga ggaggccaag acctctggtg ccccggggag cccccaaaca
      301 ccccctgagc gtcatgactc tggtggttcc ctgcccctga caccgcggat ggagagccac
      361 tcagaggatg aagatcttgc tggggctgtc ggtggcctgg gctggaacag taggagtccc
      421 cggacccaga gcccaggggg ctgctcagcg gaggctgtgc tggcccggaa gaaacaccgt
      481 cggcggccat cgaagcgcaa aaggcactgg cgaccctacc tggagctgag ctgggctgag
      541 aaacaacagc gggatgagag gcagagccag agggcctccc gggtccgcga agagatgttc
      601 gccaaaggcc agcccgtggc cccctacaac accacccagt tcctgatgaa tgacagggac
      661 ccggaggagc ccaacttgga tgtgccccat gggatctccc acccaggttc cagtggggag
      721 agtgaggccg gggacagtga tgggcggggc cgancgcacg gtgagttcca gcngnnggac
      781 ttctctgana cttacgaacg cttccacacc gagagcctgc agggccgcag caagc
//
