LOCUS       sequence-212173-        9713 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses miR-185.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9713)
  TITLE     van Oers mIR plasmids
REFERENCE   2  (bases 1 to 9713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGC-U6-miR-185
FEATURES             Location/Qualifiers
     source          1..9713
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     ncRNA           63..156
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           872..984
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        1054..2265
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     2102..2121
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     primer_bind     complement(2313..2329)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2314..2330)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             join(2332..2334,2335..2337,2338..3051)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2377..2398)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2638..2657)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2985..3006
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             3320..3789
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4489..4506)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4489..4505)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4498..4520)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4714..4733)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4833..4855
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4893..4911)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4979..5083
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5084..5152,5153..5944)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5302..5321)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6115..6703
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6604..6623
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6857..6874
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6991..7012
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7027..7032,7033..7050,7051..7057)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7065..7081
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7070..7092
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7089..7105
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7089..7105
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             7631..8100
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    8165..8522
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             8595..9011
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     8941..8963
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     8979..8995
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     9325..9341
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     promoter        9371..9389
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        join(9473..9713,1..8)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     9473..9493
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     9644..9663
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 gaaacaccgt gctcgcttcg gcagcacata tactagtcga ctagggataa cagggtaatt
       61 gtttgaatga ggcttcagta ctttacagaa tcgttgcctg cacatcttgg aaacacttgc
      121 tgggattact tcttcaggtt aacccaacag aaggctcgat aattgaattc gcgatcagaa
      181 ggctagggaa tggctagggt ttgctctcag cccacaggtg aacaggagca agaaaggggc
      241 taaagaagtg gcatagcaga agggacctca ccaagtcccg aaaagagcat cagaccctcg
      301 ggtgccggtt ggagatgcct ggcattcaca ggggcttgcc agccgcttcc tgggaaaaca
      361 tctgggctta ggtaaaggca ggatctgtta cgggagaggt atatcaggaa aaaggtatga
      421 ggagtaattt gctggccaga gacggcagag ggactgtggg gcagcgggcc tggcttgagt
      481 ggggggtgag ggattggaga gaaaggcagt tcctgatggt cccctcccag gggctggctt
      541 tcctctggtc cttctctccc aatgactgcg tcttcatcga tgctgcaacc ctgagctctg
      601 gcagcacctc caactccagg ctgcctggag acacatcgat ggccttcctg tggaccttgg
      661 agccctcctc acatctccaa cctagagggc tggtctgcta gggccagcta ggatggcagg
      721 tcctgcagct gaactagaga aacacccttg atctggctat gtctgctgat agctgcctct
      781 gccagaggtc ctacccagta cagcctaggg aggaccaaag gagtctaaga gccaaagcct
      841 cttcctgaca ccggcagctg ataggaggga attcaagggg ctactttagg agcaattatc
      901 ttgtttacta aaactgaata ccttgctatc tctttgatac atttttacaa agctgaatta
      961 aaatggtata aattaaatca cttttttcaa ttggaagact aatgcggcct cgacattgat
     1021 tattgactag taacccgtgt cggctccaga tctggcctcc gcgccgggtt ttggcgcctc
     1081 ccgcgggcgc ccccctcctc acggcgagcg ctgccacgtc agacgaaggg cgcagcgagc
     1141 gtcctgatcc ttccgcccgg acgctcagga cagcggcccg ctgctcataa gactcggcct
     1201 tagaacccca gtatcagcag aaggacattt taggacggga cttgggtgac tctagggcac
     1261 tggttttctt tccagagagc ggaacaggcg aggaaaagta gtcccttctc ggcgattctg
     1321 cggagggatc tccgtggggc ggtgaacgcc gatgattata taaggacgcg ccgggtgtgg
     1381 cacagctagt tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtg gatcgctgtg
     1441 atcgtcactt ggtgagtagc gggctgctgg gctggccggg gctttcgtgg ccgccgggcc
     1501 gctcggtggg acggaagcgt gtggagagac cgccaagggc tgtagtctgg gtccgcgagc
     1561 aaggttgccc tgaactgggg gttgggggga gcgcagcaaa atggcggctg ttcccgagtc
     1621 ttgaatggaa gacgcttgtg aggcgggctg tgaggtcgtt gaaacaaggt ggggggcatg
     1681 gtgggcggca agaacccaag gtcttgaggc cttcgctaat gcgggaaagc tcttattcgg
     1741 gtgagatggg ctggggcacc atctggggac cctgacgtga agtttgtcac tgactggaga
     1801 actcggtttg tcgtctgttg cgggggcggc agttatggcg gtgccgttgg gcagtgcacc
     1861 cgtacctttg ggagcgcgcg ccctcgtcgt gtcgtgacgt cacccgttct gttggcttat
     1921 aatgcagggt ggggccacct gccggtaggt gtgcggtagg cttttctccg tcgcaggacg
     1981 cagggttcgg gcctagggta ggctctcctg aatcgacagg cgccggacct ctggtgaggg
     2041 gagggataag tgaggcgtca gtttctttgg tcggttttat gtacctatct tcttaagtag
     2101 ctgaagctcc ggttttgaac tatgcgctcg gggttggcga gtgtgttttg tgaagttttt
     2161 taggcacctt ttgaaatgta atcatttggg tcaatatgta attttcagtg ttagactagt
     2221 aaattgtccg ctaaattctg gccgtttttg gcttttttgt tagacgaagc ttgggctgca
     2281 ggtcgagctc aagcttcgga tcctgcagtc gacgataccg tcgacctcga catggtgagc
     2341 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     2401 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     2461 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     2521 accttcacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
     2581 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     2641 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     2701 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     2761 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
     2821 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     2881 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     2941 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     3001 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta aggatccgga
     3061 ttagtccaat ttgttaaaga caggatatca gtggtccagg ctctagtttt gactcaacaa
     3121 tatcaccagc tgaagcctat agagtacgag ccatagataa aataaaagat tttatttagt
     3181 ctccagaaaa aggggggaat gaaagacccc acctgtaggt ttggcaagct agcttaagta
     3241 acgccatttt gcaaggcatg gaaaaataca taactgagaa tagagaagtt cagatcaagg
     3301 tcaggaacag atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc
     3361 tgccccggct cagggccaag aacagatgga acagctgaat atgggccaaa caggatatct
     3421 gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggtcccca gatgcggtcc
     3481 agccctcagc agtttctaga gaaccatcag atgtttccag ggtgccccaa ggacctgaaa
     3541 tgaccctgtg ccttatttga actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct
     3601 tctgctcccc gagctcaata aaagagccca caacccctca ctcggggcgc cagtcctccg
     3661 attgactgag tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt tgcatccgac
     3721 ttgtggtctc gctgttcctt gggagggtct cctctgagtg attgactacc cgtcagcggg
     3781 ggtctttcac atgcagcatg tatcaaaatt aatttggttt tttttcttaa gtatttacat
     3841 taaatggcca tagtacttaa agttacattg gcttccttga aataaacatg gagtattcag
     3901 aatgtgtcat aaatatttct aattttaaga tagtatctcc attggctttc tactttttct
     3961 tttatttttt tttgtcctct gtcttccatt tgttgttgtt gttgtttgtt tgtttgtttg
     4021 ttggttggtt ggttaatttt tttttaaaga tcctacacta tagttcaagc tagactatta
     4081 gctactctgt aacccagggt gaccttgaag tcatgggtag cctgctgttt tagccttccc
     4141 acatctaaga ttacaggtat gagctatcat ttttggtata ttgattgatt gattgattga
     4201 tgtgtgtgtg tgtgattgtg tttgtgtgtg tgattgtgta tatgtgtgta tggttgtgtg
     4261 tgattgtgtg tatgtatgtt tgtgtgtgat tgtgtgtgtg tgattgtgca tgtgtgtgtg
     4321 tgtgattgtg tttatgtgta tgattgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg
     4381 tgtgtgtgtg ttgtgtatat atatttatgg tagtgagagg caacgctccg gctcaggtgt
     4441 caggttggtt tttgagacag agtctttcac ttagcttgga attaattcac tggccgtcgt
     4501 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     4561 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     4621 gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg
     4681 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     4741 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     4801 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     4861 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     4921 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     4981 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     5041 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     5101 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     5161 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     5221 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     5281 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     5341 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     5401 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     5461 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     5521 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     5581 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     5641 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     5701 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     5761 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     5821 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     5881 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     5941 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     6001 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     6061 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     6121 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     6181 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     6241 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     6301 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     6361 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     6421 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     6481 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     6541 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     6601 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     6661 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     6721 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     6781 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     6841 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     6901 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     6961 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     7021 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     7081 atttcacaca ggaaacagct atgaccatga ttacgccaag ctttgctctt aggagtttcc
     7141 taatacatcc caaactcaaa tatataaagc atttgacttg ttctatgccc tagggggcgg
     7201 ggggaagcta agccagcttt ttttaacatt taaaatgtta attccatttt aaatgcacag
     7261 atgtttttat ttcataaggg tttcaatgtg catgaatgct gcaatattcc tgttaccaaa
     7321 gctagtataa ataaaaatag ataaacgtgg aaattactta gagtttctgt cattaacgtt
     7381 tccttcctca gttgacaaca taaatgcgct gctgagaagc cagtttgcat ctgtcaggat
     7441 caatttccca ttatgccagt catattaatt actagtcaat tagttgattt ttatttttga
     7501 catatacatg tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt
     7561 tgcaaggcat ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca
     7621 gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc
     7681 tcagggccaa gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc
     7741 agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag
     7801 cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt
     7861 gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc
     7921 cgagctcaat aaaagagccc acaacccctc actcggcgcg ccagtcctcc gattgactga
     7981 gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct
     8041 cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca
     8101 tttgggggct cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg
     8161 aggtaagctg gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt
     8221 ttatgcgcct gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg
     8281 gaactgacga gttcggaaca cccggccgca accctgggag acgtcccagg gacttcgggg
     8341 gccgtttttg tggcccgacc tgagtccaaa aatcccgatc gttttggact ctttggtgca
     8401 ccccccttag aggagggata tgtggttctg gtaggagacg agaacctaaa acagttcccg
     8461 cctccgtctg aatttttgct ttcggtttgg gaccgaagcc gcgccgcgcg tcttgtctgc
     8521 tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc tgaaaatatg
     8581 ggcccgccgc ggcgggccag actgttacca ctcccttaag tttgacctta ggtcactgga
     8641 aagatgtcga gcggatcgct cacaaccagt cggtagatgt caagaagaga cgttgggtta
     8701 ccttctgctc tgcagaatgg ccaaccttta acgtcggatg gccgcgagac ggcaccttta
     8761 accgagacct catcacccag gttaagatca aggtcttttc acctggcccg catggacacc
     8821 cagaccaggt cccctacatc gtgacctggg aagccttggc ttttgacccc cctccctggg
     8881 tcaagccctt tgtacaccct aagcctccgc ctcctcttcc tccatccgcc ccgtctctcc
     8941 cccttgaacc tcctcgttcg accccgcctc gatcctccct ttatccagcc ctcactcctt
     9001 ctctaggcgc ccccatatga gatcttatat ggggcacccc cgccccttgt aaacttccct
     9061 gaccctgaca tgacaagagt tactaacagc ccctctctcc aagctcactt acaggctctc
     9121 tacttagtcc agcacgaagt ctggagacct ctggcggcag cctaccaaga acaactggac
     9181 cgaccggtgg tacctcaccc ttaccgagtc ggcgacacag tgtgggtccg ccgacaccag
     9241 actaagaacc tagaacctcg ctggaaagga ccttacacag tcctgctgac cacccccacc
     9301 gccctcaaag tagacggcat cgcagcttgg atacacgccg cccacgtaaa acaattgaag
     9361 atctagtcga aattaaccct cactaaaggg aacaaaagct ggagctccac cgcggtggcg
     9421 gccgtgacgg agcgtgaccg cgcgccgagc gcgcgccagg gtcgggcagg aagagggcct
     9481 atttcccatg attccttcat atttgcatat acgatacaag gctgttagag agataattag
     9541 aattaatttg actgtaaaca caaagatatt agtacaaaat acgtgacgta gaaagtaata
     9601 atttcttggg tagtttgcag ttttaaaatt atgttttaaa atggactatc atatgcttac
     9661 cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag gac
//
