LOCUS       sequence-212177-        9708 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expressed miR-128.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9708)
  TITLE     van Oers mIR plasmids
REFERENCE   2  (bases 1 to 9708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGC-U6-miR-128
FEATURES             Location/Qualifiers
     source          1..9708
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        84..332
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     84..104
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     255..274
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     ncRNA           387..480
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           1191..1303
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        1373..2584
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     2421..2440
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     primer_bind     complement(2632..2648)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2633..2649)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             join(2651..2653,2654..2656,2657..3370)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2696..2717)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2957..2976)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3304..3325
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             3639..4108
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4808..4825)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4808..4824)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4817..4839)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5033..5052)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5152..5174
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5212..5230)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5298..5402
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5403..5471,5472..6263)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5621..5640)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6434..7022
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6923..6942
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7176..7193
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7310..7331
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7346..7351,7352..7369,7370..7376)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7384..7400
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7389..7411
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7408..7424
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7408..7424
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             7950..8419
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    8484..8841
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             8914..9330
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     9260..9282
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     9298..9314
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     9644..9660
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     promoter        9690..9708
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gaacaaaagc tggagctcca ccgcggtggc ggccgtgacg gagcgtgacc gcgcgccgag
       61 cgcgcgccag ggtcgggcag gaagagggcc tatttcccat gattccttca tatttgcata
      121 tacgatacaa ggctgttaga gagataatta gaattaattt gactgtaaac acaaagatat
      181 tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat
      241 tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg
      301 ctttatatat cttgtggaaa ggacgaaaca ccgtgctcgc ttcggcagca catatactag
      361 tcgactaggg ataacagggt aattgtttga atgaggcttc agtactttac agaatcgttg
      421 cctgcacatc ttggaaacac ttgctgggat tacttcttca ggttaaccca acagaaggct
      481 cgataattga attctgtctt actgttaata actgcagtca tgcaagcagc tatttcaaaa
      541 aatctgctag ttcttaattg caatactagt aactaattat ttcaactctt agcatacgta
      601 taggatagta tatacagtat aatacataca ctataaaaac ttgaaagaaa tggaccaaga
      661 aatagtgacg ttagcaatga caggtttata ggccctgact ctttgatacg tagctgcttt
      721 cattcttgga ctcttttgaa aagtttgcag cttctcctta tgtgcttata ttttacaata
      781 attggccttc ttcctgagct gttggattcg gggccgtagc actgtctgag aggtttacat
      841 ttctcacagt gaaccggtct ctttttcagc tgcttcctga cttcttttta cttctttttg
      901 cttttttgct ttttttttct atgctatatt aagatactaa tatttgttta tattttccat
      961 tgcctataat taccttttaa ttttgcatca tactcagaaa taaggatttg aatttaagta
     1021 acaaccttgg cttcatacaa tttatcctta aaaattcaaa ttatctgttg ctttcatttc
     1081 ttttccaaag catatttgga tgctttttta agattcaatt tggtttgcat cttatctaac
     1141 agagtttaac atcttaagca tgccaaactc attaccccgg gaagggcgaa ttcaaggggc
     1201 tactttagga gcaattatct tgtttactaa aactgaatac cttgctatct ctttgataca
     1261 tttttacaaa gctgaattaa aatggtataa attaaatcac ttttttcaat tggaagacta
     1321 atgcggcctc gacattgatt attgactagt aacccgtgtc ggctccagat ctggcctccg
     1381 cgccgggttt tggcgcctcc cgcgggcgcc cccctcctca cggcgagcgc tgccacgtca
     1441 gacgaagggc gcagcgagcg tcctgatcct tccgcccgga cgctcaggac agcggcccgc
     1501 tgctcataag actcggcctt agaaccccag tatcagcaga aggacatttt aggacgggac
     1561 ttgggtgact ctagggcact ggttttcttt ccagagagcg gaacaggcga ggaaaagtag
     1621 tcccttctcg gcgattctgc ggagggatct ccgtggggcg gtgaacgccg atgattatat
     1681 aaggacgcgc cgggtgtggc acagctagtt ccgtcgcagc cgggatttgg gtcgcggttc
     1741 ttgtttgtgg atcgctgtga tcgtcacttg gtgagtagcg ggctgctggg ctggccgggg
     1801 ctttcgtggc cgccgggccg ctcggtggga cggaagcgtg tggagagacc gccaagggct
     1861 gtagtctggg tccgcgagca aggttgccct gaactggggg ttggggggag cgcagcaaaa
     1921 tggcggctgt tcccgagtct tgaatggaag acgcttgtga ggcgggctgt gaggtcgttg
     1981 aaacaaggtg gggggcatgg tgggcggcaa gaacccaagg tcttgaggcc ttcgctaatg
     2041 cgggaaagct cttattcggg tgagatgggc tggggcacca tctggggacc ctgacgtgaa
     2101 gtttgtcact gactggagaa ctcggtttgt cgtctgttgc gggggcggca gttatggcgg
     2161 tgccgttggg cagtgcaccc gtacctttgg gagcgcgcgc cctcgtcgtg tcgtgacgtc
     2221 acccgttctg ttggcttata atgcagggtg gggccacctg ccggtaggtg tgcggtaggc
     2281 ttttctccgt cgcaggacgc agggttcggg cctagggtag gctctcctga atcgacaggc
     2341 gccggacctc tggtgagggg agggataagt gaggcgtcag tttctttggt cggttttatg
     2401 tacctatctt cttaagtagc tgaagctccg gttttgaact atgcgctcgg ggttggcgag
     2461 tgtgttttgt gaagtttttt aggcaccttt tgaaatgtaa tcatttgggt caatatgtaa
     2521 ttttcagtgt tagactagta aattgtccgc taaattctgg ccgtttttgg cttttttgtt
     2581 agacgaagct tgggctgcag gtcgagctca agcttcggat cctgcagtcg acgataccgt
     2641 cgacctcgac atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     2701 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     2761 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     2821 ctggcccacc ctcgtgacca ccttcaccta cggcgtgcag tgcttcagcc gctaccccga
     2881 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     2941 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     3001 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     3061 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
     3121 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt
     3181 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     3241 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga
     3301 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     3361 gtacaagtaa ggatccggat tagtccaatt tgttaaagac aggatatcag tggtccaggc
     3421 tctagttttg actcaacaat atcaccagct gaagcctata gagtacgagc catagataaa
     3481 ataaaagatt ttatttagtc tccagaaaaa ggggggaatg aaagacccca cctgtaggtt
     3541 tggcaagcta gcttaagtaa cgccattttg caaggcatgg aaaaatacat aactgagaat
     3601 agagaagttc agatcaaggt caggaacaga tggaacagct gaatatgggc caaacaggat
     3661 atctgtggta agcagttcct gccccggctc agggccaaga acagatggaa cagctgaata
     3721 tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga
     3781 tggtccccag atgcggtcca gccctcagca gtttctagag aaccatcaga tgtttccagg
     3841 gtgccccaag gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc
     3901 tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa aagagcccac aacccctcac
     3961 tcggggcgcc agtcctccga ttgactgagt cgcccgggta cccgtgtatc caataaaccc
     4021 tcttgcagtt gcatccgact tgtggtctcg ctgttccttg ggagggtctc ctctgagtga
     4081 ttgactaccc gtcagcgggg gtctttcaca tgcagcatgt atcaaaatta atttggtttt
     4141 ttttcttaag tatttacatt aaatggccat agtacttaaa gttacattgg cttccttgaa
     4201 ataaacatgg agtattcaga atgtgtcata aatatttcta attttaagat agtatctcca
     4261 ttggctttct actttttctt ttattttttt ttgtcctctg tcttccattt gttgttgttg
     4321 ttgtttgttt gtttgtttgt tggttggttg gttaattttt ttttaaagat cctacactat
     4381 agttcaagct agactattag ctactctgta acccagggtg accttgaagt catgggtagc
     4441 ctgctgtttt agccttccca catctaagat tacaggtatg agctatcatt tttggtatat
     4501 tgattgattg attgattgat gtgtgtgtgt gtgattgtgt ttgtgtgtgt gattgtgtat
     4561 atgtgtgtat ggttgtgtgt gattgtgtgt atgtatgttt gtgtgtgatt gtgtgtgtgt
     4621 gattgtgcat gtgtgtgtgt gtgattgtgt ttatgtgtat gattgtgtgt gtgtgtgtgt
     4681 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt tgtgtatata tatttatggt agtgagaggc
     4741 aacgctccgg ctcaggtgtc aggttggttt ttgagacaga gtctttcact tagcttggaa
     4801 ttaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     4861 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     4921 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt
     4981 ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct
     5041 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
     5101 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     5161 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
     5221 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     5281 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     5341 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     5401 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     5461 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     5521 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     5581 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     5641 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     5701 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     5761 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     5821 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     5881 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     5941 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     6001 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     6061 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     6121 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     6181 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     6241 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     6301 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     6361 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     6421 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     6481 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     6541 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     6601 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     6661 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     6721 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     6781 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     6841 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     6901 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     6961 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     7021 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     7081 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     7141 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     7201 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     7261 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     7321 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     7381 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     7441 tttgctctta ggagtttcct aatacatccc aaactcaaat atataaagca tttgacttgt
     7501 tctatgccct agggggcggg gggaagctaa gccagctttt tttaacattt aaaatgttaa
     7561 ttccatttta aatgcacaga tgtttttatt tcataagggt ttcaatgtgc atgaatgctg
     7621 caatattcct gttaccaaag ctagtataaa taaaaataga taaacgtgga aattacttag
     7681 agtttctgtc attaacgttt ccttcctcag ttgacaacat aaatgcgctg ctgagaagcc
     7741 agtttgcatc tgtcaggatc aatttcccat tatgccagtc atattaatta ctagtcaatt
     7801 agttgatttt tatttttgac atatacatgt gaaagacccc acctgtaggt ttggcaagct
     7861 agcttaagta acgccatttt gcaaggcatg gaaaaataca taactgagaa tagaaaagtt
     7921 cagatcaagg tcaggaacag atggaacagc tgaatatggg ccaaacagga tatctgtggt
     7981 aagcagttcc tgccccggct cagggccaag aacagatgga acagctgaat atgggccaaa
     8041 caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggtcccca
     8101 gatgcggtcc agccctcagc agtttctaga gaaccatcag atgtttccag ggtgccccaa
     8161 ggacctgaaa tgaccctgtg ccttatttga actaaccaat cagttcgctt ctcgcttctg
     8221 ttcgcgcgct tctgctcccc gagctcaata aaagagccca caacccctca ctcggcgcgc
     8281 cagtcctccg attgactgag tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt
     8341 tgcatccgac ttgtggtctc gctgttcctt gggagggtct cctctgagtg attgactacc
     8401 cgtcagcggg ggtctttcat ttgggggctc gtccgggatc gggagacccc tgcccaggga
     8461 ccaccgaccc accaccggga ggtaagctgg ccagcaactt atctgtgtct gtccgattgt
     8521 ctagtgtcta tgactgattt tatgcgcctg cgtcggtact agttagctaa ctagctctgt
     8581 atctggcgga cccgtggtgg aactgacgag ttcggaacac ccggccgcaa ccctgggaga
     8641 cgtcccaggg acttcggggg ccgtttttgt ggcccgacct gagtccaaaa atcccgatcg
     8701 ttttggactc tttggtgcac cccccttaga ggagggatat gtggttctgg taggagacga
     8761 gaacctaaaa cagttcccgc ctccgtctga atttttgctt tcggtttggg accgaagccg
     8821 cgccgcgcgt cttgtctgct gcagcatcgt tctgtgttgt ctctgtctga ctgtgtttct
     8881 gtatttgtct gaaaatatgg gcccgccgcg gcgggccaga ctgttaccac tcccttaagt
     8941 ttgaccttag gtcactggaa agatgtcgag cggatcgctc acaaccagtc ggtagatgtc
     9001 aagaagagac gttgggttac cttctgctct gcagaatggc caacctttaa cgtcggatgg
     9061 ccgcgagacg gcacctttaa ccgagacctc atcacccagg ttaagatcaa ggtcttttca
     9121 cctggcccgc atggacaccc agaccaggtc ccctacatcg tgacctggga agccttggct
     9181 tttgaccccc ctccctgggt caagcccttt gtacacccta agcctccgcc tcctcttcct
     9241 ccatccgccc cgtctctccc ccttgaacct cctcgttcga ccccgcctcg atcctccctt
     9301 tatccagccc tcactccttc tctaggcgcc cccatatgag atcttatatg gggcaccccc
     9361 gccccttgta aacttccctg accctgacat gacaagagtt actaacagcc cctctctcca
     9421 agctcactta caggctctct acttagtcca gcacgaagtc tggagacctc tggcggcagc
     9481 ctaccaagaa caactggacc gaccggtggt acctcaccct taccgagtcg gcgacacagt
     9541 gtgggtccgc cgacaccaga ctaagaacct agaacctcgc tggaaaggac cttacacagt
     9601 cctgctgacc acccccaccg ccctcaaagt agacggcatc gcagcttgga tacacgccgc
     9661 ccacgtaaaa caattgaaga tctagtcgaa attaaccctc actaaagg
//
