LOCUS       sequence-212181-        9333 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  express SST2 from the GAL1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9333)
  AUTHORS   Harrigan P, Madhani HD, El-Samad H
  TITLE     Real-Time Genetic Compensation Defines the Dynamic Demands of 
            Feedback Control.
  JOURNAL   Cell. 2018 Oct 18;175(3):877-886.e10. doi: 
            10.1016/j.cell.2018.09.044.
  PUBMED    30340045
REFERENCE   2  (bases 1 to 9333)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.09"; journalName: "Cell"; date: "2018-10-18- 
            18"; volume: "175"; issue: "3"; pages: "877-886.e10"
COMMENT     Sequence Label: pHES944
FEATURES             Location/Qualifiers
     source          1..9333
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2162..2603
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    2162..2279
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     2574..2597
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     complement(4722..4738)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4722..4738)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(5880..5899)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5881..5899)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5906..5923)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5906..5922)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5915..5937)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6063..6518
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6150..6169)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6360..6381
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6874..6893)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6993..7015
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7053..7071)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7156..7260
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7261..7329,7330..8121)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7479..7498)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8292..8880
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8781..8800
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9034..9051
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9168..9189
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9204..9209,9210..9227,9228..9234)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9242..9258
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9247..9269
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9266..9282
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9266..9282
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        9303..9321
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ggccggccat ttaaatgttt aaacggccac gctggccata tttctaccaa tctctcaaca
       61 ctgagtaatg gtagttataa gaaagagacc gagttaggga cagttagagg cggtggagat
      121 attccttatg gcatgtctgg cgatgataaa acttttcaaa cggcagcccc gatctaaaag
      181 agctgacagg gaaatggtca gaaaaagaaa cgtgcacccg cccgtctgga cgcgccgctc
      241 acccgcacgg cagagaccaa tcagtaaaaa tcaacggtta acgacattac tatatatata
      301 atataggaag catttaatag aacagcatcg taatatatgt gtactttgca gttatgacgc
      361 cagatggcag tagtggaaga tattctttat tgaaaaatag cttgtcacct tacgtacaat
      421 cttgatccgg agcccgtcgc tttgagagaa atcagaagat tccaaaagtc tactgaactt
      481 ttgatcagaa agttgccttt ccaaagacta gtcagagaaa tcgcccaaga tttcaagacc
      541 gatctaagat tccaatcctc tgccatcggt gctctacagg aatccgttga agcttacttg
      601 gtctctttgt tcgaagacac caacttggcc gctatccacg ctaagcgtgt taccatccaa
      661 aagaaggata tcaagttggc tagaagattg agaggtgaaa gatcctaagc acacagggat
      721 tgttgtacaa ttacttttta tgattttaga tacacacaaa tcttttctac gattaccttt
      781 catttatttt cgtttcatgt tttattaatc attctttttg tatatttcac atcgggtggg
      841 gtttttatat taaattttaa taacataatc ttttaaagtt tctactttct atcctgtata
      901 ttatttccat atctatatct atattttcaa gcttgttcac atccttatcg gaattgttcc
      961 agcgtttttg tacagaaaat gatgttatgg taacgagcta tatcagacct actagtctta
     1021 tagagctgta cagagtcttg gaaacataac caacacacca acaagatgtc atttgattcg
     1081 ttactcgaca agaatgataa gctggtaaaa gtttgcggga ttcaaaccgt cgaggctgcc
     1141 gaaactgcgc ttcaagcagg cgctgatttg atagggatca tatgtgtccc caacaggaag
     1201 cggactatcg agagcgctgt ggctcgtgaa atatccaaat tgattcacaa atcaggtact
     1261 acaaagctgg tgggggtgtt caggaatcaa tctgttgagg acgtacatcg gctttctgag
     1321 gaatatgacc ttgacataat ccaattacat ggtgatgaat catggccaga gtactataac
     1381 gtcattaaga aaccaataat caaaagagtc atattcccta gagatgtcga tgttgtaaca
     1441 caagtgtgtc aaagaaaacc cttggtatgt ctaccattgt tcgactccga ggcaggcggt
     1501 acaggtgaaa agctggactg gtccagtatt tcctcctggg caagcgaaca gaataacgta
     1561 ttttatatac tagcgggtgg actaactgca gaaaacgtca tggaggcggt ggggttgcct
     1621 ggtgtaattg gtgttgacgt ctcaggtggt gtagagactg acggcgtcaa agacaatgac
     1681 aagataatta aatatgtaca aaatgcaaag aaacaatgaa ttacgaagag aagtctcaat
     1741 gaggttttac aaacaatcct ataatacata tatgccactt agatatactt tgaatactga
     1801 atatactgac tcattatatt ctttaattgt agtttctcaa aaatattcat acaggttagc
     1861 tatgaattca tgctataatt tccattttgt tcatgtaaga tacacatagg gataaaatgt
     1921 gataactaat cagcggtacc gggcccttat attgaatttt caaaaattct tacttttttt
     1981 ttggatggac gcaaagaagt ttaataatca tattacatgg cattaccacc atatacatat
     2041 ccatatctaa tcttacttat atgttgtgga aatgtaaaga gccccattat cttagcctaa
     2101 aaaaaccttc tctttggaac tttcagtaat acgcttaact gctcattgct atattgaagt
     2161 acggattaga agccgccgag cgggcgacag ccctccgacg gaagactctc ctccgtgcgt
     2221 cctcgtcttc accggtcgcg ttcctgaaac gcagatgtgc ctcgcgccgc actgctccga
     2281 acaataaaga ttctacaata ctagctttta tggttatgaa gaggaaaaat tggcagtaac
     2341 ctggccccac aaaccttcaa attaacgaat caaattaaca accataggat gataatgcga
     2401 ttagtttttt agccttattt ctggggtaat taatcagcga agcgatgatt tttgatctat
     2461 taacagatat ataaatggaa aagctgcata accactttaa ctaatacttt caacattttc
     2521 agtttgtatt acttcttatt caaatgtcat aaaagtatca acaaaaaatt gttaatatac
     2581 ctctatactt taacgtcaag gagaaaaaac tatactcgag atggtggata aaaataggac
     2641 gttgcatgaa ttatcttcca aaaatttcag cagaacgccg aatggactga tctttaccaa
     2701 tgatttaaag acagtatatt ccatttttct aatttgtttg gatttgaaag agaaaaaaca
     2761 tagtagtgac actaagtctt tcttattaac tgcattcact aaacatttcc acttcacttt
     2821 tacatatcag gaagctatta aagcaatggg acaattggaa ctaaaagtgg atatgaacac
     2881 aacttgtatt aacgtttcat ataatattaa acctagtttg gcgcgccacc ttcttacatt
     2941 atttatgtct tctaagctac tacatacacc gcaggacaga actcgtggcg agccaaaaga
     3001 gaaagttctt ttccaaccaa cacctaaggg agttgctgtt ttacaaaagt atgttagaga
     3061 tattgggttg aaaacaatgc ctgatattct tttgtcatcg tttaactcca tgaaactttt
     3121 cacttttgag agaagctctg tcacggattc aatcatccat agtgattatt tgatccatat
     3181 tcttttcatt aaaatgatgg gcgctaagcc aaatgtttgg tctccaacaa acgcagatga
     3241 cccattacct tgcctatctt cgttgttgga atacactaat aatgatgata catttacttt
     3301 tgagaaatct aagccagaac aaggatggca agctcagatt ggaaacatcg atataaatga
     3361 cttagaaagg gtatcgccat tagctcatcg attctttaca aatccagatt ctgagtcgca
     3421 cacgcaatac tacgtttcga atgcaggtat taggttgttt gaaaataaga cgtttggcac
     3481 ttccaaaaaa atcgtgatta agtacacctt tacaacaaag gctatatggc agtggataat
     3541 ggactgtact gatattatgc atgttaaaga agcggtctcg ttggcagcgt tatttttaaa
     3601 aacggggttg attgtaccag ttcttttaca accatcacgc actgacaaga aaaagtttca
     3661 aattagcagg tcctcttttt ttaccttgtc caagcgtggt tgggatttgg tttcttggac
     3721 aggttgtaag agcaataata ttagagcacc aaatggtagc accatagacc tagatttcac
     3781 attaaggggt catatgactg ttcgtgatga aaaaaagaca ttggatgata gtgaaggttt
     3841 ttctcaagac atgcttatct cttcgagtaa tttaaataaa ctggactatg tgctaactga
     3901 tccaggtatg agatatttat tcaggcgcca tttagaaaag gaactttgtg tagaaaattt
     3961 ggatgtcttc attgagatta agaggttttt gaagaaaatg acaattctga aaaaattgat
     4021 agattctaag cattgtgata aaaaatctaa taccagtact tcaaaaaaca atatcgtaaa
     4081 aacgattgat tcagctttaa tgaaacaggc caatgagtgc cttgaaatgg catatcacat
     4141 ttattcgtcc tatatcatga taggatcacc atatcaatta aatatccatc ataacttgcg
     4201 tcagaatatt tctgacatca tgttgcaccc tcattctcca ttatcggaac actttcctac
     4261 taatctatac gatccatcac ctgcatccgc ggaatcagca gcttcatcaa ttagctcaac
     4321 ggaggctgac acgttaggtg aaccacctga agtgagtcta aaaccttcaa aaaatctttc
     4381 aaatgaaaac tgttcattta agaagcaagg attcaaacat cagttaaagg aatataaacc
     4441 tgccccgtta actctggcag aaacgcattc gcctaatgca tcagtagaaa attcacatac
     4501 aattgttcgt tatggcatgg acaacaccca aaatgatacc aaatcggttg attcatttcc
     4561 agcaactttg aaggtcctca ggaaattata tcccctcttc gagatcgtta gcaatgaaat
     4621 gtatagactg atgaataacg attcctttca aaaatttacc cagtcagacg tatacaaaga
     4681 tgctagcgct ttaatagaaa tccaagaaaa gtgctaataa ggatccacta gttctagaca
     4741 cctgcaacat gcgtgagtaa ataagcggcc gccaccgcgg tggagctcta agcaaatagc
     4801 taaattatat acgaattaat attatgatta agtgtttacg tgagtgcgat atttttatta
     4861 ctatcttata cagttgtata tactctataa aatgagttgt ctattaatta acgcgatgaa
     4921 tgctttctgg gtttacctct ccaacaactc tagtttactt ctcaatacat tcaattgtat
     4981 ttgatttgtc aatacttcat cattaatcaa ttctatagtt ttgtttttct cgtttatttc
     5041 caaatttaat gcatcaattt tattattcaa tttgtcgttg attttggtta atgattttat
     5101 ggtttgatct ctggcattga ttgtttgtgt tagtttttca ttattgataa ttaaattatt
     5161 taagttagtt atcaactcgg tgttttcaag tttcaagttt tcaatttctt tagagtttat
     5221 tagatttgtc aaagtttctg aattgcttga ttggtcctgt agaagagtat ttgttgttgt
     5281 ggataattga ttcaattttt gagacaattg ctggaaggcg ttgaaatatc tagcatcaat
     5341 ctcatggttt ttttcccgag agtctcgtag attcaattgt tttaatatat cttgggacca
     5401 ctcttgattt gaactcatgg aaattaaact gggtgttgtg ttgtggtgta atgattgtac
     5461 cccctttgct tataattgtg tggttccgat gctgacttac tgggtattat atgtgtgccc
     5521 aatagaaaga gaacaattga cccggttatt gcaaggaaaa tttcaagtct tgtaaaagca
     5581 tataaaaata gttcaggcac tccgaaatac ttggttggcg tgtttcgtaa tcaacctaag
     5641 gaggatgttt tggctctggt caatgattac ggcattgata tcgtccaact gcatggagat
     5701 gagtcgtggc gagaatacca agagttcctc ggtttgccag ttattaaaag actcgtattt
     5761 ccaaaagact gcaacatact actcagtgca gcttcacaga aacctcattc gtttattccc
     5821 ttgtttgatt cagaaggccg gccatttaaa tgtttaaacg gccacgctgg cccaattcgc
     5881 cctatagtga gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     5941 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     6001 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     6061 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     6121 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     6181 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     6241 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     6301 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     6361 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     6421 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     6481 taacgcgaat tttaacaaaa tattaacgct tacaatttcc tgatgcggta ttttctcctt
     6541 acgcatctgt gcggtatttc acaccgcata gggtaataac tgatataatt aaattgaagc
     6601 tctaatttgt gagtttagta ttaggatctc tacccttggc gaaaagtcct ctgccaacaa
     6661 tgatgatatc tgatccacca cttacaactt cgtcgacggt tctgtactgc tgacccaata
     6721 tgcgtatata taccaatcta agtctgtgct ccttccttcg ttcttccttc tgttcggaga
     6781 ttaccgaatc aaaaaaattt caaggaaacc gaaatcaaaa aaaagaataa aaaaaaaatg
     6841 atgaattgaa aaggtggtat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     6901 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     6961 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     7021 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     7081 taatgtcatg ataataatgg tttcttagac gtcggcgcgc cgaggacgtc aggtggcact
     7141 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     7201 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     7261 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     7321 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     7381 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     7441 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     7501 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     7561 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     7621 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     7681 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     7741 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     7801 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     7861 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     7921 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     7981 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     8041 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     8101 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     8161 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     8221 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     8281 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     8341 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     8401 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     8461 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     8521 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     8581 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     8641 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     8701 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     8761 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     8821 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     8881 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     8941 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     9001 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     9061 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     9121 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     9181 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcctat gttgtgtgga
     9241 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctc
     9301 ggaattaacc ctcactaaag ggaacaaaag ctg
//
