LOCUS       sequence-212182-       10192 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  blue light inducible cryptochrome based optogenetic expression 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10192)
  AUTHORS   Harrigan P, Madhani HD, El-Samad H
  TITLE     Real-Time Genetic Compensation Defines the Dynamic Demands of 
            Feedback Control.
  JOURNAL   Cell. 2018 Oct 18;175(3):877-886.e10. doi: 
            10.1016/j.cell.2018.09.044.
  PUBMED    30340045
REFERENCE   2  (bases 1 to 10192)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.09"; journalName: "Cell"; date: "2018-10-18- 
            18"; volume: "175"; issue: "3"; pages: "877-886.e10"
COMMENT     Sequence Label: pHES945
FEATURES             Location/Qualifiers
     source          1..10192
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        276..683
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     promoter        2839..3482
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     3375..3398
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     CDS             3495..3935
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             3951..5444
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     primer_bind     complement(6739..6758)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6740..6758)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6765..6782)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6765..6781)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6774..6796)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6922..7377
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7009..7028)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7219..7240
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7733..7752)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7852..7874
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7912..7930)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8015..8119
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8120..8188,8189..8980)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8338..8357)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9151..9739
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9640..9659
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9893..9910
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10027..10048
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10063..10068,10069..10086,10087..10093)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10101..10117
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10106..10128
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10125..10141
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10125..10141
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        10162..10180
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ggccggccat ttaaatgttt aaacggccac gctggccgag gagaacttct agtatatcta
       61 catacctaat attattgcct tattaaaaat ggaatcccaa caattacatc aaaatccaca
      121 ttctcttcaa aatcaattgt cctgtacttc cttgttcatg tgtgttcaaa aacgttatat
      181 ttataggata attatactct atttctcaac aagtaattgg ttgtttggcc gagcggtcta
      241 aggcgcctga ttcaagaaat atcttgaccg cagttaactg tgggaatact caggtatcgt
      301 aagatgcaag agttcgaatc tcttagcaac cattattttt ttcctcaaca taacgagaac
      361 acacaggggc gctatcgcac agaatcaaat tcgatgactg gaaatttttt gttaatttca
      421 gaggtcgcct gacgcatata cctttttcaa ctgaaaaatt gggagaaaaa ggaaaggtga
      481 gagcgccgga accggctttt catatagaat agagaagcgt tcatgactaa atgcttgcat
      541 cacaatactt gaagttgaca atattattta aggacctatt gttttttcca ataggtggtt
      601 agcaatcgtc ttactttcta acttttctta ccttttacat ttcagcaata tatatatata
      661 tatttcaagg atataccatt ctaatgtctg cccctaagtt accaatagat agcggtttaa
      721 tataaggttt caacaagaaa tattttgtga aaatcaaaag ttaatagttt cactgtcgaa
      781 aatatgaaag tttaagtttt cagttcatcc cattgtagtt atcaaatttc tgtgtttccc
      841 gtatatgtgt gctctgtatt catcgctatc ttgaaactgg ttatgatcgt tgcgcatgtg
      901 acctgtatcg ctcaaaagag cccggcaccg gtaccgggca aaagccccta gcaagctaaa
      961 atgattcaac tttcgtagta atttgttcaa ccggtcacag taaagtcaat gactaacttt
     1021 tgaatcgagg atgtatttcc aattgaaagc aatgtatata taaaccattg tgttgaagct
     1081 tgtcttacag ttttcttgta tcctcccatt gttcatatag tacttaaagt accataatcc
     1141 ttaaagaaca aacttgaaag agaatacact atacacaact aataaataaa gaataatcat
     1201 ggctgtgacc aagacaattg tagttctacc aggtgaccat gttggtcaag aaatcactga
     1261 agaggccatt aaagttttaa atgctattca ggaatgtcgt ccagacaagg tcaatttcaa
     1321 gtttgatcat catttgatcg gtggtgctgc aattgatgcc actggtgttc cattaccaga
     1381 cgaagctttg gaggcctcta agaaagctga tgccgtgctt ctgggtgctg ttggtggtcc
     1441 aaaatggggt actggcgctg tcagaccaga acaaggtctt ttgaaaatcc gtaaggagtt
     1501 gcaattgtat gctaatctaa gaccatgtaa ttttgcatct gattccttac tagatctatc
     1561 gccattgaag cctgaaattg caagaggtac agatttcgtc gttgttagag aactagtggg
     1621 tggtatttat ttcggggaga gaaaagaaga tgaaggtgat ggtgtcgcct gggatagcga
     1681 aaagtattct gtgcccgaag ttcaaagaat cactagaatg gctgctttca tggctctgca
     1741 acacaaccca cctttaccta tttggtctct agataaagcc aacgttctag catcatcaag
     1801 attatggaga aaaacagtgg aagagacgat taagaatgaa ttcccacaat tgactgttca
     1861 acatcaattg attgattccg ctgctatgat cttggttaag aacccaactc atctaaatgg
     1921 tattattatc acaaacaata tgtttggtga tattatctcc gatgaagcct ccgtgatccc
     1981 aggttcgtta ggattacttc catctgcctc cttggcatct cttccagata agaatacggc
     2041 atttggttta tatgagccat gtcatggctc tgctccagac ttaccaaagg gaaaggttaa
     2101 ccccgtagca acaatcctat ctgctgctat gatgttgaag ttgtctctgg acctgttcga
     2161 agaaggtgaa attatagaac aagcagtcaa gaaagtgttg gattctggta tcagaacagc
     2221 ggatctaaag ggtaccaact ctactactga ggtcggtgat gcagtagcga aagctgtcag
     2281 ggaactatta gcttagactt agtagatgta cagttgcata cataagtctg aacagaaaga
     2341 agtaaatatc atatctagtt cggtttagaa atcagtgact atattggttt tgaccagatc
     2401 gcaatccttg caagcattta gtaatgacta tcaaaccggt gaatcatctg ataaggctat
     2461 attagcttgt gcattcgcat gtatcgggaa acgaacggta ccgggccctt ttcgaggacc
     2521 ttgtcacctt gagcccaaga gagccaagat ttaaattttc ctatgacttg atgcaaattc
     2581 ccaaagctaa taacatgcaa gacacgtacg gtcaagaaga catatttgac ctcttaacag
     2641 gttcagacgc gactgcctca tcagtaagac ccgttgaaaa gaacttacct gaaaaaaacg
     2701 aatatatact agcgttgaat gttagcgtca acaacaagaa gtttaatgac gcggaggcca
     2761 aggcaaaaag attccttgat tacgtaaggg agttagaatc attttgaata aaaaacacgc
     2821 tttttcagtt cgagtttatc attatcaata ctgccatttc aaagaatacg taaataatta
     2881 atagtagtga ttttcctaac tttatttagt caaaaaatta gccttttaat tctgctgtaa
     2941 cccgtacatg cccaaaatag ggggcgggtt acacagaata tataacatcg taggtgtctg
     3001 ggtgaacagt ttattcctgg catccactaa atataatgga gcccgctttt taagctggca
     3061 tccagaaaaa aaaagaatcc cagcaccaaa atattgtttt cttcaccaac catcagttca
     3121 taggtccatt ctcttagcgc aactacagag aacaggggca caaacaggca aaaaacgggc
     3181 acaacctcaa tggagtgatg caacctgcct ggagtaaatg atgacacaag gcaattgacc
     3241 cacgcatgta tctatctcat tttcttacac cttctattac cttctgctct ctctgatttg
     3301 gaaaaagctg aaaaaaaagg ttgaaaccag ttccctgaaa ttattcccct acttgactaa
     3361 taagtatata aagacggtag gtattgattg taattctgta aatctatttc ttaaacttct
     3421 taaattctac ttttatagtt agtctttttt ttagttttaa aacaccaaga acttagtttc
     3481 gactcgacaa taaaatgaag ctactgtctt ctatcgaaca agcatgcgat atttgccgac
     3541 ttaaaaagct caagtgctcc aaagaaaaac cgaagtgcgc caagtgtctg aagaacaact
     3601 gggagtgtcg ctactctccc aaaaccaaaa ggtctccgct gactagggca catctgacag
     3661 aagtggaatc aaggctagaa agactggaac agctatttct actgattttt cctcgagaag
     3721 accttgacat gattttgaaa atggattctt tacaggatat aaaagcattg ttaacaggat
     3781 tatttgtaca agataatgtg aataaagatg ccgtcacaga tagattggct tcagtggaga
     3841 ctgatatgcc tctaacattg agacagcata gaataagtgc gacatcatca tcggaagaga
     3901 gtagtaacaa aggtcaaaga cagttgactg tatcgccgct cgaggccacc atgaagatgg
     3961 acaaaaagac tatagtttgg tttagaagag acctaaggat tgaggataat cctgcattag
     4021 cagcagctgc tcacgaagga tctgtttttc ctgtcttcat ttggtgtcct gaagaagaag
     4081 gacagtttta tcctggaaga gcttcaagat ggtggatgaa acaatcactt gctcacttat
     4141 ctcaatcctt gaaggctctt ggatctgacc tcactttaat caaaacccac aacacgattt
     4201 cagcgatctt ggattgtatc cgcgttaccg gtgctacaaa agtcgtcttt aaccacctct
     4261 atgatcctgt ttcgttagtt cgggaccata ccgtaaagga gaagctggtg gaacgtggga
     4321 tctctgtgca aagctacaat ggagatctat tgtatgaacc gtgggagata tactgcgaaa
     4381 agggcaaacc ttttacgagt ttcaattctt actggaagaa atgcttagat atgtcgattg
     4441 aatccgttat gcttcctcct ccttggcggt tgatgccaat aactgcagcg gctgaagcga
     4501 tttgggcgtg ttcgattgaa gaactagggc tggagaatga ggccgagaaa ccgagcaatg
     4561 cgttgttaac tagagcttgg tctccaggat ggagcaatgc tgataagtta ctaaatgagt
     4621 tcatcgagaa gcagttgata gattatgcaa agaacagcaa gaaagttgtt gggaattcta
     4681 cttcactact ttctccgtat ctccatttcg gggaaataag cgtcagacac gttttccagt
     4741 gtgcccggat gaaacaaatt atatgggcaa gagataagaa cagtgaagga gaagaaagtg
     4801 cagatctttt tcttagggga atcggtttaa gagagtattc tcggtatata tgtttcaact
     4861 tcccgtttac tcacgagcaa tcgttgttga gtcatcttcg gtttttccct tgggatgctg
     4921 atgttgataa gttcaaggcc tggagacaag gcaggaccgg ttatccgttg gtggatgccg
     4981 gaatgagaga gctttgggct accggatgga tgcataacag aataagagtg attgtttcaa
     5041 gctttgctgt gaagtttctt ctccttccat ggaaatgggg aatgaagtat ttctgggata
     5101 cacttttgga tgctgatttg gaatgtgaca tccttggctg gcagtatatc tctgggagta
     5161 tccccgatgg ccacgagctt gatcgcttgg acaatcccgc gttacaaggc gccaaatatg
     5221 acccagaagg tgagtacata aggcaatggc ttcccgagct tgcgagattg ccaactgaat
     5281 ggatccatca tccatgggac gctcctttaa ccgtactcaa agcttctggt gtggaactcg
     5341 gaacaaacta tgcgaaaccc attgtagaca tcgacacagc tcgtgagcta ctagctaaag
     5401 ctatttcaag aacccgtgaa gcacagatca tgatcggagc agcataataa gcggccgcca
     5461 ccgcggtgga gctctaagca aatagctaaa ttatatacga attaatatta tgattaagtg
     5521 tttacgtgag tgcgatattt ttattactat cttatacagt tgtatatact ctataaaatg
     5581 agttgtctat taattaacgc gatgaatgct ttctgggttt acctctccaa caactctagt
     5641 ttacttctca atacattcaa ttgtatttga tttgtcaata cttcatcatt aatcaattct
     5701 atagttttgt ttttctcgtt tatttccaaa tttaatgcat caattttatt attcaatttg
     5761 tcgttgattt tggttaatga ttttatggtt tgatctctgg cattgattgt ttgtgttagt
     5821 ttttcattat tgataattaa attatttaag ttagttatca actcggtgtt ttcaagtttc
     5881 aagttttcaa tttctttaga gtttattaga tttgtcaaag tttctgaatt gcttgattgg
     5941 tcctgtagaa gagtatttgt tgttgtggat aattgattca atttttgaga caattgctgg
     6001 aaggcgttga aatatctagc atcaatctca tggttttttt cccgagagtc tcgtagattc
     6061 aattgtttta atatatcttg ggaccactct tgatttgaac tcatggaaat taaactgggt
     6121 gttgtgttgt ggtgtaatga ttgtaccccc tttgcttata attgtgtggc caccgaagtc
     6181 ggtgatgctg tcgccgaaga agttaagaaa atccttgctt aaaaagattc tcttttttta
     6241 tgatatttgt acataaactt tataaatgaa attcataata gaaacgacac gaaattacaa
     6301 aatggaatat gttcataggg tagacgaaac tatatacgca atctacatac atttatcaag
     6361 aaggagaaaa aggaggatgt aaaggaatac aggtaagcaa attgatacta atggctcaac
     6421 gtgataagga aaaagaattg cactttaaca ttaatattga caaggaggag ggcaccacac
     6481 aaaaagttag gtgtaacaga aaatcatgaa actatgattc ctaatttata tattggagga
     6541 ttttctctaa aaaaaaaaaa atacaacaaa taaaaaacac tcaatgacct gaccatttga
     6601 tggagtttaa gtcaatacct tcttgaacca tttcccataa tggtgaaagt tccctcaaga
     6661 attttactct gtcagaaacg gccttaacga cgtaggccgg ccatttaaat gtttaaacgg
     6721 ccacgctggc ccaattcgcc ctatagtgag tcgtattaca attcactggc cgtcgtttta
     6781 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     6841 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     6901 cgcagcctga atggcgaatg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     6961 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     7021 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     7081 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     7141 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     7201 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     7261 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     7321 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttcct
     7381 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatag ggtaataact
     7441 gatataatta aattgaagct ctaatttgtg agtttagtat taggatctct acccttggcg
     7501 aaaagtcctc tgccaacaat gatgatatct gatccaccac ttacaacttc gtcgacggtt
     7561 ctgtactgct gacccaatat gcgtatatat accaatctaa gtctgtgctc cttccttcgt
     7621 tcttccttct gttcggagat taccgaatca aaaaaatttc aaggaaaccg aaatcaaaaa
     7681 aaagaataaa aaaaaaatga tgaattgaaa aggtggtatg gtgcactctc agtacaatct
     7741 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
     7801 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     7861 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
     7921 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcggcgcgcc
     7981 gaggacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     8041 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     8101 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     8161 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     8221 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     8281 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     8341 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     8401 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     8461 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     8521 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     8581 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     8641 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     8701 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     8761 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     8821 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     8881 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     8941 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     9001 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     9061 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     9121 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     9181 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     9241 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     9301 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     9361 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     9421 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     9481 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     9541 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     9601 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     9661 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     9721 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     9781 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     9841 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     9901 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     9961 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
    10021 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
    10081 ggctcctatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
    10141 ccatgattac gccaagctcg gaattaaccc tcactaaagg gaacaaaagc tg
//
