LOCUS       sequence-212190-        1842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Two SpyCatchers linked by an elastin-like polypeptide with an RGDSP 
            integrin-binding site, an MMP-cleavable sequence and a C-terminal 
            SnoopCatcher.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Wieduwild R, Howarth M
  TITLE     Assembling and decorating hyaluronan hydrogels with twin protein 
            superglues to mimic cell-cell interactions.
  JOURNAL   Biomaterials. 2018 Oct;180:253-264. doi: 
            10.1016/j.biomaterials.2018.07.020. Epub 2018 Jul 17.
  PUBMED    30053659
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biomaterials."; date: "2018-10"; volume: "180:253-264. doi"; pages:
            " 10.1016/j.biomaterials.2018.07.020. Epub 2018 Jul 17"
COMMENT     Sequence Label: pQE80L TriCatcher-RGDSP-MMP
FEATURES             Location/Qualifiers
     source          1..1842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..33
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             52..72
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             79..417
                     /codon_start=1
                     /product="truncated version of the CnaB2 adhesin domain of 
                     Streptococcus pyogenes FbaB"
                     /label=SpyCatcher
                     /note="forms a covalent isopeptide bond with the SpyTag 
                     peptide (Zakeri et al., 2012)"
                     /translation="VDTLSGLSSEQGQSGDMTIEEDSATHIKFSKRDEDGKELAGATME
                     LRDSSGKTISTWISDGQVKDFYLYPGKYTFVETAAPDGYEVATAITFTVNEQGQVTVNG
                     KATKGDAHI"
     CDS             1018..1038
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1045..1383
                     /codon_start=1
                     /product="truncated version of the CnaB2 adhesin domain of 
                     Streptococcus pyogenes FbaB"
                     /label=SpyCatcher
                     /note="forms a covalent isopeptide bond with the SpyTag 
                     peptide (Zakeri et al., 2012)"
                     /translation="VDTLSGLSSEQGQSGDMTIEEDSATHIKFSKRDEDGKELAGATME
                     LRDSSGKTISTWISDGQVKDFYLYPGKYTFVETAAPDGYEVATAITFTVNEQGQVTVNG
                     KATKGDAHI"
     CDS             1504..1839
                     /label=SnoopCatcher
ORIGIN
        1 atgaaaggca gcagccatca tcatcatcat cacgtcgaca tcccaacgac cgaaaacctg
       61 tattttcagg gcgccatggt tgatacctta tcaggtttat caagtgagca aggtcagtcc
      121 ggtgatatga caattgaaga agatagtgct acccatatta aattctcaaa acgtgatgag
      181 gacggcaaag agttagctgg tgcaactatg gagttgcgtg attcatctgg taaaactatt
      241 agtacatgga tttcagatgg acaagtgaaa gatttctacc tgtatccagg aaaatataca
      301 tttgtcgaaa ccgcagcacc agacggttat gaggtagcaa ctgctattac ctttacagtt
      361 aatgagcaag gtcaggttac tgtaaatggc aaagcaacta aaggtgacgc tcatattgac
      421 ggtagcggtg gctctggcgg tcagctcgac ggccacggcg tgggtgttcc gggcgtcggt
      481 gtgccgggtg tgggtgtgcc gggcgaaggt gtgccgggcg tcggtgtgcc gggtgttggt
      541 gttccgggcg ttggtgtgcc gggcgttggc gtgccgggcg agggtgtgcc gggcgttggt
      601 gttccgggcg tgggtgtgcc gggcgtgggc gtgccgggcg tcggtgttcc gggcgagggt
      661 gtgccgggcg taggtgtgcc gggcgttggt gagctctatg cgcgtggtga tagtccggcc
      721 gttccgctca gtctctatag tggtatccgt gccactagtg tgccgggcgt cggcgtgccg
      781 ggcgtaggtg ttccgggcga gggtgttccg ggcgttggtg tgccgggcgt cggcgtgccg
      841 ggcgtgggtg ttccgggcgt aggtgtgccg ggcgagggtg tgccgggcgt gggcgtgccg
      901 ggcgtaggtg ttccgggcgt aggtgttccg ggcgtaggtg ttccgggtga aggcgtgccg
      961 ggcgttggtg tgccgggtgt gggcgtgccg ggcgggctgc tcgacatccc aacgaccgaa
     1021 aacctgtatt ttcagggcgc catggttgat accttatcag gtttatcaag tgagcaaggt
     1081 cagtccggtg atatgacaat tgaagaagat agtgctaccc atattaaatt ctcaaaacgt
     1141 gatgaggacg gcaaagagtt agctggtgca actatggagt tgcgtgattc atctggtaaa
     1201 actattagta catggatttc agatggacaa gtgaaagatt tctacctgta tccaggaaaa
     1261 tatacatttg tcgaaaccgc agcaccagac ggttatgagg tagcaactgc tattaccttt
     1321 acagttaatg agcaaggtca ggttactgta aatggcaaag caactaaagg tgacgctcat
     1381 attggatccc ccgccaacct gaaggccctg gaggcccaga agcagaagga gcagagacag
     1441 gccgccgagg agctggccaa cgccaagaag ctgaaggagc agctggagaa gggatcccat
     1501 atgaagccgc tgcgtggtgc cgtgtttagc ctgcagaaac agcatcccga ctatcccgat
     1561 atctatggcg cgattgatca gaatgggacc tatcaaaatg tgcgtaccgg cgaagatggt
     1621 aaactgacct ttaagaatct gagcgatggc aaatatcgcc tgtttgaaaa tagcgaaccc
     1681 gctggctata aaccggtgca gaataagccg attgtggcgt ttcagattgt gaatggcgaa
     1741 gtgcgtgatg tgaccagcat tgtgccgcag gatattccgg ctacatatga atttaccaac
     1801 ggtaaacatt atatcaccaa tgaaccgata ccgccgaaat aa
//
