LOCUS       sequence-212213-       12358 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses UBE3B fused to BirA in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12358)
  AUTHORS   Braganza A, Li J, Zeng X, Yates NA, Dey NB, Andrews J, Clark J, 
            Zamani L, Wang XH, St Croix C, O'Sullivan R, Garcia-Exposito L, 
            Brodsky JL, Sobol RW
  TITLE     UBE3B is a Calmodulin-Regulated, Mitochondria-Associated E3 
            Ubiquitin Ligase.
  JOURNAL   J Biol Chem. 2016 Dec 21. pii: jbc.M116.766824. doi: 
            10.1074/jbc.M116.766824.
  PUBMED    28003368
REFERENCE   2  (bases 1 to 12358)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2016 Dec 21. pii: jbc.M116.766824. doi: 
            10.1074/jbc.M116.766824."
COMMENT     Sequence Label: pLVX-BirA-UBE3B-Puro
FEATURES             Location/Qualifiers
     source          1..12358
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    680..805
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1302..1535
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1720..1764
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2027..2142
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2200..2503
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2504..2707
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2658..2678
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2704..2728
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2823..2847
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(2868..3218,3219..3221,3222..3830)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     protein_bind    complement(7051..7075)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(7107..7681,7682..7684,7685..7693)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7274..7291)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7501..7520
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7713..8312
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7713..7732)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8209..8229
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8326..8914
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8379..8399)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8797..8808)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9121..9754
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9882..9898)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9882..9898)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9895..9917)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9906..9922
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9930..9936,9937..9954,9955..9960))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9975..9996
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10113..10130)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10284..10872)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10364..10383)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11043..11834,11835..11903))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11666..11685
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11904..12008)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    12056..12190
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12093..12112)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12147..12166
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(12358,1..633)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
       61 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
      121 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
      181 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
      241 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
      301 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
      361 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
      421 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
      481 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      541 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
      601 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
      661 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
      721 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
      781 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
      841 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
      901 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
      961 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     1021 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     1081 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     1141 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     1201 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1261 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1321 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1381 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1441 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1501 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1561 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1621 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1681 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1741 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1801 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1861 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1921 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1981 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccttta aaagaaaagg
     2041 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2101 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2161 cagcagagat ccagtttatc gataagcttg ggagttccgc gttacataac ttacggtaaa
     2221 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     2281 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     2341 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     2401 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     2461 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
     2521 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
     2581 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
     2641 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
     2701 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     2761 ccatagaaga caccgactct actagaggat ctatttccgg tgaattcctc gagactagat
     2821 caacaagttt gtacaaaaaa gcaggctccg cggccgcccc cttcaccatg aaggacaaca
     2881 ccgtgcccct gaagctgatc gccctgctgg ccaatggcga gttccactct ggcgagcagc
     2941 tgggcgagac actgggcatg tctagagccg ccatcaacaa gcacatccag accctgagag
     3001 actggggcgt ggacgtgttc accgtgcctg gcaagggcta cagcctgccc gagcctatcc
     3061 agctgctgaa cgccaagcag atcctgggcc agctggatgg cggatctgtg gctgtgctgc
     3121 ccgtgatcga cagcaccaac cagtacctgc tggaccggat cggcgagctg aaaagcggcg
     3181 acgcctgtat cgccgagtat cagcaggctg gcagaggcgg caggggcaga aagtggttct
     3241 ctcccttcgg cgccaatctg tacctgagca tgttctggcg gctggaacag ggacctgccg
     3301 ccgctattgg actgagcctc gtgatcggca tcgtgatggc cgaggtgctg agaaagctgg
     3361 gcgccgacaa agtgcgcgtg aagtggccca acgacctgta tctgcaggac cggaagctgg
     3421 ccggcatcct ggtggaactg acaggcaaaa caggcgacgc tgcccagatc gtgattggcg
     3481 ctggcatcaa tatggccatg cggcgggtgg aagagtccgt cgtgaatcag ggctggatca
     3541 ccctgcagga agccggcatc aacctggaca gaaacaccct ggccgccatg ctgatcagag
     3601 agctgagagc cgccctggaa ctgttcgagc aggaaggcct ggccccctac ctgtccagat
     3661 gggagaagct ggacaacttc atcaacagac ccgtgaaact gatcatcggc gacaaagaga
     3721 tcttcggcat cagccggggc atcgacaagc agggcgctct gctgctggaa caggacggca
     3781 tcatcaagcc ctggatgggc ggcgagatca gcctgagatc tgccgagaaa ttcaccctgt
     3841 ctcagacctc gagagcatgg ttcatcgata gagcccgtca ggcacgagaa gaaaggcttg
     3901 tgcagaagga acgggagcgg gcagctgttg tgatccaggc ccatgtccgg agttttctct
     3961 gtcggagtcg actgcagaga gatatcagga gagagattga tgactttttt aaagcagatg
     4021 accctgagtc cactaaaaga agtgcacttt gtattttcaa gattgccagg aaactgctgt
     4081 tcctattcag aatcaaagag gataatgaga gatttgagaa gttgtgtcgc agcatcctga
     4141 gcagcatgga tgctgagaat gagcctaagg tgtggtatgt gtccctggct tgttctaagg
     4201 acctcaccct cctttggatt caacagatca agaacatttt gtggtactgc tgtgattttc
     4261 tcaagcagct caagcctgaa atcctgcagg actcccgact catcaccctg tacctcacga
     4321 tgcttgtcac cttcacagac acttcaacgt ggaaaattct tcggggaaaa ggtgaaagtc
     4381 ttcgaccagc gatgaaccac atttgtgcaa atataatggg acatctcaac cagcatggat
     4441 tttattctgt gctgcagata ttgttaaccc gtggcctggc aagaccccgt ccttgtctat
     4501 ccaaaggcac tttaacagca gctttttctc tagcgttacg ccctgtgatt gctgcacagt
     4561 tctcagacaa tctgattcgg ccgttcctca tccacatcat gtctgtgcct gctctggtga
     4621 ctcatctcag cacagtgacc cctgagcgcc tcactgtttt agaatcccat gacatgcttc
     4681 gtaaattcat catattttta agagaccaag atcgatgccg tgatgtatgt gaaagtttag
     4741 aaggatgcca tacgctttgt ctaatgggca acctcctaca cttgggctcc ctcagcccca
     4801 gagtgttaga ggaggagaca gatgggttcg tgagtttgct cacccagacg ctgtgctact
     4861 gtcggaagta tgtgtctcag aagaagtcca acctgaccca ctggcatcct gtccttggct
     4921 ggttctccca atctgtggat tatggcctta acgagtcaat gcacttgatc accaaacagc
     4981 tgcagttctt gtggggggtg cctctgatcc ggatcttctt ctgtgacatc ctgagcaaga
     5041 agctactgga gagccaggag ccagcccacg cacagccagc atcccctcag aatgtgctcc
     5101 cagtgaagag tctcctaaag cgtgcttttc aaaagtcggc atcagtccgg aatattctca
     5161 ggcctgtcgg gggtaaacgg gtcgactctg cagaagtcca gaaggtttgc aacatctgtg
     5221 tcctctacca gacctcgctg acaactctca cacagattcg gctgcagata ctcacaggtc
     5281 tcacttacct tgatgacctg cttcccaaac tgtgggcatt tatctgtgag ctcgggcccc
     5341 acggagggtt aaagctcttc ttggaatgcc tgaacaatga cactgaagag tccaagcaac
     5401 tcttggccat gctgatgctg ttctgtgact gttcgcggca cctcatcaca atccttgatg
     5461 acattgaagt ttatgaagaa cagatttcat tcaaactgga agagctggtc actatctcct
     5521 ctttcctgaa ttcttttgtg tttaagatga tctgggatgg aattgtagag aacgccaagg
     5581 gtgagacctt ggagctgttc cagtctgtcc acgggtggct tatggtgctg tacgagcggg
     5641 actgccggcg gcgcttcacc cccgaggacc actggctgcg aaaggatctc aaacctagcg
     5701 tgctcttcca agaactcgac agggacagaa aacgggcaca gttgatcctg cagtacatcc
     5761 cacatgtcat ccctcacaaa aacagagttc tactgtttcg aaccatggtt accaaggaga
     5821 aggagaaact ggggctggtg gaaaccagct ctgcctcccc gcatgtcact cacatcacca
     5881 tccgccggtc caggatgctg gaggacggct acgagcagct taggcagctc tcccagcacg
     5941 ccatgaaggg ggtcatccgt gtgaagtttg tcaatgacct cggggtggac gaagcaggga
     6001 ttgatcaaga cggtgttttt aaggagttct tggaagagat catcaagaga gtttttgacc
     6061 cagcactcaa tctgttcaag acaaccagtg gggatgagag gctgtacccc tcacccacat
     6121 cctacatcca tgagaattac ctgcagctct tcgagtttgt ggggaagatg ctggggaagg
     6181 ctgtgtatga gggaattgtg gtggacgtgc catttgcatc cttcttcctg agccaactgc
     6241 ttgggcacca ccacagcgtc ttctatagct cggtggatga actgccttct ctggactccg
     6301 agttctataa aaacctcacc tccatcaagc gctatgatgg ggacatcact gacctgggcc
     6361 tgacgctgtc ttacgacgag gacgtcatgg gtcagcttgt ttgccatgaa ctgattcctg
     6421 gagggaagac cattcctgtt acaaatgaaa ataaaattag ctacatccat ctgatggcac
     6481 attttcgaat gcacactcaa ataaaaaacc aaacagctgc cctcattagc ggattccgtt
     6541 ccattatcaa acccgagtgg atccgaatgt tctcaactcc tgaactgcag cgtctcatct
     6601 ctggcgacaa tgctgagatt gatctggaag atttaaagaa gcacacagtc tactacggtg
     6661 gtttccatgg aagtcacaga gtcatcatct ggctctggga tattctggcc tccgacttca
     6721 caccggatga gagagctatg tttctgaagt tcgtgaccag ctgctccaga cccccgctcc
     6781 tgggattcgc ctacctcaag cctccattct ccatccgctg cgtggaggtg tcggacgatc
     6841 aggacaccgg ggacactctg ggcagcgtcc tccggggctt cttcaccatc cgcaagcggg
     6901 agccaggcgg ccgcctgccc acctcctcca cctgcttcaa cctgctcaag ctgcccaact
     6961 acagcaagaa gagcgtcctc cgcgagaagc tgcgctacgc catcagcatg aacacgggct
     7021 ttgaactctc ctagaagggt gggcgcgccg acccagcttt cttgtacaaa gtggttgatc
     7081 tagttctaga gcggccgcgg atcccgcccc tctccctccc ccccccctaa cgttactggc
     7141 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg
     7201 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct
     7261 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca
     7321 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg
     7381 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct
     7441 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa
     7501 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt
     7561 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa
     7621 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa
     7681 gcttgccaca acccacaagg agacgacctt ccatgaccga gtacaagccc acggtgcgcc
     7741 tcgccacccg cgacgacgtc ccccgggccg tacgcaccct cgccgccgcg ttcgccgact
     7801 accccgccac gcgccacacc gtcgacccgg accgccacat cgagcgggtc accgagctgc
     7861 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
     7921 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
     7981 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
     8041 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
     8101 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
     8161 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
     8221 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
     8281 cctggtgcat gacccgcaag cccggtgcct agacgcgtct ggaacaatca acctctggat
     8341 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     8401 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     8461 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     8521 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     8581 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     8641 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     8701 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc
     8761 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     8821 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     8881 acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttaattctgc agtcgagacc
     8941 tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct
     9001 ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa
     9061 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aagaggggac
     9121 tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca
     9181 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
     9241 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
     9301 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
     9361 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
     9421 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
     9481 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
     9541 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
     9601 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     9661 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     9721 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     9781 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggccttg acattgctag
     9841 cgtttaccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     9901 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     9961 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
    10021 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
    10081 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
    10141 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
    10201 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
    10261 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
    10321 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
    10381 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
    10441 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
    10501 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
    10561 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
    10621 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
    10681 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
    10741 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
    10801 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
    10861 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
    10921 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
    10981 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
    11041 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
    11101 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
    11161 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
    11221 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
    11281 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
    11341 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
    11401 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
    11461 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
    11521 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
    11581 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
    11641 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
    11701 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
    11761 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
    11821 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
    11881 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
    11941 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
    12001 gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg agatcaactt
    12061 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
    12121 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
    12181 tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata
    12241 ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga
    12301 attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagtt
//
