LOCUS       sequence-212236-        9169 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9169)
  AUTHORS   Tsuruzoe K, Emkey R, Kriauciunas KM, Ueki K, Kahn CR
  TITLE     Insulin receptor substrate 3 (IRS-3) and IRS-4 impair IRS-1- and 
            IRS-2-mediated signaling.
  JOURNAL   Mol Cell Biol. 2001 Jan . 21(1):26-38.
  PUBMED    11113178
REFERENCE   2  (bases 1 to 9169)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2001 Jan . 21(1):26-38."
COMMENT     Sequence Label: pBABE puro mouse IRS-2 myc
FEATURES             Location/Qualifiers
     source          1..9169
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..470
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    535..734
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             935..1351
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1281..1303
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1319..1335
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             5448..5477
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(5492..5512)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5497..5826
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5677..5812
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5836..6435
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5836..5855)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6332..6352
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6656..7125
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     rep_origin      complement(7426..8014)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7506..7525)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8174..8965,8966..9034))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8797..8816
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9035..9139)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
       61 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
      121 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
      181 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
      241 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
      301 aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga gtcgcccggg
      361 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
      421 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct
      481 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggcaagctg
      541 gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct
      601 gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga
      661 gttctgaaca cccggccgca accctgggag acgtcccagg gactttgggg gccgtttttg
      721 tggcccgacc tgaggaaggg agtcgatgtg gaatccgacc ccgtcaggat atgtggttct
      781 ggtaggagac gagaacctaa aacagttccc gcctccgtct gaatttttgc tttcggtttg
      841 gaaccgaagc cgcgcgtctt gtctgctgca gcgctgcagc atcgttctgt gttgtctctg
      901 tctgactgtg tttctgtatt tgtctgaaaa ttagggccag actgttacca ctcccttaag
      961 tttgacctta ggtcactgga aagatgtcga gcggatcgct cacaaccagt cggtagatgt
     1021 caagaagaga cgttgggtta ccttctgctc tgcagaatgg ccaaccttta acgtcggatg
     1081 gccgcgagac ggcaccttta accgagacct catcacccag gttaagatca aggtcttttc
     1141 acctggcccg catggacacc cagaccaggt cccctacatc gtgacctggg aagccttggc
     1201 ttttgacccc cctccctggg tcaagccctt tgtacaccct aagcctccgc ctcctcttcc
     1261 tccatccgcc ccgtctctcc cccttgaacc tcctcgttcg accccgcctc gatcctccct
     1321 ttatccagcc ctcactcctt ctctaggcgc cggccggatc ccccgggctg caggaatttt
     1381 aagttagcgc ttatgggagc cgcccccgcc cgcgcccctc ggccctcgcc atcccctgtt
     1441 cgcccagccg ggcagagaga cctgaagcgg cggcgatggc tagcgcgccc ctgcctgggc
     1501 cccccgcgtc ggcgggcggg gacggcccga acctcaataa caacaacaac aacaacaacc
     1561 acagcgtgcg caagtgcggc tacctgcgca agcagaagca cggccacaag cgctttttcg
     1621 tgttgcgcgg ccccggcacg ggcggcgacg aggcatccgc ggctgggggg tcgccgccgc
     1681 agcctccgcg gctggagtac tacgagagcg agaagaagtg gaggagcaag gcgggcgcgc
     1741 cgaagcgagt gatcgcgctc gactgctgtc tgaacatcaa caagcgcgcg gacgccaagc
     1801 acaagtacct gatcgccctc tacaccaagg acgagtactt cgctgtagcg gcggagaacg
     1861 agcaggagca ggagggctgg taccgcgcac tcaccgactt ggtcagcgaa ggccgctctg
     1921 gcgagggggg ctcgggcacc accggaggct cttgcagcgc ctctctcccg ggcgtcctgg
     1981 gcggctcatc agcgggcgcc gctggctgcg atgacaacta cgggctcgtg acacccgcca
     2041 cggccgtcta ccgcgaggtg tggcaggtga acctgaaacc taagggactg ggccagagca
     2101 agaacctgac tggtgtatac cgcctatgcc tgtctgcgcg caccatcggc ttcgtgaagc
     2161 tcaattgcga acagccgtcg gtgacgctgc agcttatgaa cattcgccgc tgcggccact
     2221 cggacagctt cttcttcatc gaggtgggcc gttcggccgt caccggtccc ggggagctgt
     2281 ggatgcaagc cgacgactcg gtggtggcgc agaacatcca tgagaccatc ctagaagcta
     2341 tgaaggcact caaagagctc ttcgagttcc ggcctcgcag caagagtcag tcgtccgggt
     2401 cgtcagccac gcatcccatc agcgtgccgg gcgcgcgccg ccaccaccac ctagtcaacc
     2461 taccccctag ccagaccggc ctggtgcgcc gctcgcgcac tgacagcctg gcggccaccc
     2521 ccccagcagc caagtgcact tcgtgccggg ttcgtacggc cagcgagggc gacggcggcg
     2581 cggcaggcgg ggccgggacg gcaggaggca ggccgatgtc ggtggcagag agccccctga
     2641 gtcccgggcc ggtgcgcgcg ccccttagcc gctcgcacac cctgagcgcc ggctgcggag
     2701 gccgcccgag caaagtgact ctggcgccgg cagggggagc cctgcaacac agccgctcca
     2761 tgtccatgcc cgtggcgcac tcacctcctg cagccaccag cccaggcagc ctgtcctcca
     2821 gcagtgggca cggctcgggc tcctacccgc tgccacctgg ctcccacccg cacctgcctc
     2881 atccactgca tcacccccaa ggccagcgtc cgtccagcgg tagtgcctcc gcctcgggct
     2941 cccccagcga cccgggtttc atgtcccttg acgagtatgg ctccagccct ggcgacctga
     3001 gagccttcag tagccacagg agcaacacac ccgagtcaat agcggagacc ccgccagcca
     3061 gagatggcag tgggggcgaa ctctatgggt acatgagcat ggatagaccc ctgagccact
     3121 gtggccgccc ttaccgtagg gtctcagggg atggggccca ggacctggat agaggactga
     3181 ggaagaggac ttattcccta accacgcctg ccaggcagcg gcaggtacct cagccttcct
     3241 ctgcctctct agatgaatac actctcatga gggccacctt ctctggtagt tcaggtcgcc
     3301 tctgcccatc cttccctgcg tcctctccca aagtggccta caacccttac ccagaggact
     3361 atggagacat tgagattggt tctcacaaga gttccagcag taacctgggg gcagatgatg
     3421 gctacatgcc catgacccct ggggcagccc ttaggagtgg tggtcccaat agctgcaaga
     3481 gcgatgacta catgcccatg agccccacaa gcgtgtctgc tcccaagcag atcctgcagc
     3541 cacgcttggc agcggccttg cccccttccg gagcagccgt gccagcaccc ccttcagggg
     3601 tgggcaggac cttcccagta aacggaggtg gctacaaagc cagctcccca gcggagagct
     3661 ccccagaaga cagtgggtac atgcgaatgt ggtgtggctc caagctgtct atggagaacc
     3721 cagaccctaa gctactcccc aacggggact acctcaacat gtcccccagc gaggcaggca
     3781 ctgcagggac cccacctgac ttctcagcag ctttgcgtgg aggcagtgaa ggcctcaaag
     3841 gcatcccggg ccactgctac agctctttgc cccgctctta taaggctccc tgttcctgca
     3901 gcggagacaa tgaccagtat gtgctcatga gctcccctgt gggccggatc ttggaagagg
     3961 agagactgga gccccaggcc accccagggg ctggcacctt tggggcagct ggtggtagtc
     4021 atacccagcc tcatcactca gcagtgcctt cctccatgag gccgagtgcc atcggtggcc
     4081 gccctgaggg cttcctgggc cagcgatgtc gggcagtgcg gcctacacgc ctatcgctag
     4141 agggactgca gacccttccc agcatgcaag agtaccctct acccacagag cccaagagcc
     4201 ctggcgagta catcaacatt gactttggtg aggcaggtac ccgtctgtct ccgcctgccc
     4261 ccccactact ggcatccgcg gcctcatctt cttcactgct ctcagctagt agtcctgctt
     4321 catccctggg ttcaggaacc ccaggcacca gcagcgacag ccggcagcgc tctccactct
     4381 ctgactatat gaacctggac ttcagttctc ccaagtcccc caagcctagc acccgcagtg
     4441 gggacacagt aggctccatg gatggccttc tctctccaga ggcttcatcc ccatacccac
     4501 cactgccccc acgtccttcc acttcccctt cctccttaca gcagcctctg ccacctgccc
     4561 cgggagacct ataccgcctg cctccagcat cagctgccac ttcccagggt cccactgctg
     4621 gctcctcaat gtcctccgag cctggggata atggtgacta taccgagatg gcctttggtg
     4681 tggctgcaac cccgccacaa cctatcgtgg cacctccaaa gccagaaggt gcccgagtgg
     4741 ccagtcccac atcgggcttg aagcggctaa gtctcatgga tcaggtatct ggggtggagg
     4801 ctttccttca agtcagccag ccccctgacc cccaccgggg tgctaaggtc atccgtgcag
     4861 acccacaggg gggacgtcgt cgccacagtt cagagacctt ttcctctacc accaccgtca
     4921 ccccagtgtc cccatccttt gcccacaatt ccaagcgcca caattcggcc tctgtggaaa
     4981 atgtctcact caggaaaagc agtgaaggca gcagtaccct gggaggaggt gatgagccgc
     5041 ccacatcccc aggacaggca cagcccttgg tggctgtgcc cccagtgcca caggctaggc
     5101 cgtggaaccc cggtcagccc ggagctttga ttggctgtcc tggaggcagc agttctccca
     5161 tgcgcagaga gacctccgtg ggtttccaga acggcctcaa ctatatcgcc atcgatgtga
     5221 gaggcgagca ggggtccttg gcgcagtctc agccgcagcc aggagacaag aactcctgga
     5281 gccggacccg tagccttggg gggctcctcg gcaccgtcgg aggctctggc gccagcggag
     5341 tgtgtggggg tccaggcact ggagctttgc cctctgccag cacctatgca agcatcgact
     5401 tcctgtccca tcacttgaag gaagccacag tcgtgaaaga ggttaacgag caaaagctaa
     5461 ttagcgagga ggacctttag gtcgaccctg tggaatgtgt gtcagttagg gtgtggaaag
     5521 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     5581 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     5641 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     5701 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     5761 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     5821 tgcaaaaagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     5881 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     5941 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     6001 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     6061 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     6121 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     6181 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     6241 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     6301 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     6361 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     6421 aagcccggtg cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga
     6481 ccccatgcat cgataaaata aaagatttta tttagtctcc agaaaaaggg gggaatgaaa
     6541 gaccccacct gtaggtttgg caagctagct taagtaacgc cattttgcaa ggcatggaaa
     6601 aatacataac tgagaataga gaagttcaga tcaaggtcag gaacagatgg aacagctgaa
     6661 tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca
     6721 gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc
     6781 tcagggccaa gaacagatgg tccccagatg cggtccagcc ctcagcagtt tctagagaac
     6841 catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta
     6901 accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag
     6961 agcccacaac ccctcactcg gggcgccagt cctccgattg actgagtcgc ccgggtaccc
     7021 gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg ttccttggga
     7081 gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcacatgc agcatgtatc
     7141 aaaattaatt tggttttttt tcttaagtat ttacattaaa tggccatagt tgcattaatg
     7201 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     7261 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     7321 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     7381 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     7441 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     7501 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     7561 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     7621 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     7681 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     7741 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     7801 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     7861 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     7921 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     7981 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     8041 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     8101 aaggatcttc acctagatcc ttttgcggcc gcaaatcaat ctaaagtata tatgagtaaa
     8161 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     8221 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     8281 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     8341 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     8401 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     8461 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     8521 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     8581 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     8641 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     8701 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     8761 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     8821 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     8881 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     8941 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     9001 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     9061 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     9121 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgcagc
//
