LOCUS       sequence-21225-e         761 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Sharma N, Low SH, Misra S, Pallavi B, Weimbs T
  TITLE     Apical targeting of syntaxin 3 is essential for epithelial cell 
            polarity.
  JOURNAL   J Cell Biol. 2006 Jun 19. 173(6):937-48.
  PUBMED    16785322
REFERENCE   2  (bases 1 to 761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2006 Jun 19. 173(6):937-48."
COMMENT     Sequence Label: Syn3D27-146
FEATURES             Location/Qualifiers
     source          1..761
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    22..40
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    43..61
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     71..95
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             464..475
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cgtggggcnt tagcagagct ctccctatca gtgatagaga tctccctatc agtgatagag
       61 atcgtcgacg agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt
      121 ttgacctcca tagaagacac cgggaccgat ccagcctccg gactctagcg tttaaactta
      181 agcttggtac cgagctcgga tccactagtc cagtgtggtg gaattcagga tgcgggaccg
      241 tctggagcag ctgaaggcca agcagctgac acaggatgat gatactgatg cggttgagat
      301 tgctatcgac aacacgctcg acgggcgaat ccagcggcag ctcgaaatta ctggcaaaaa
      361 gacaaccgat gaggagctgg aggagatgtt ggagagtggc aacccggcca tcttcacttc
      421 tgggatcatt gactcacaga tttccaagca agccctcagt gagattgagg gacgacacaa
      481 ggacattgtg aggctggaga gcagcatcaa ggagcttcac gacatgttta tggacatcgc
      541 catgctggtg gagaatcagg gtgagatgtt agataacata gagttgaatg tcatgcacac
      601 agtggaccac gtggagaagg cacgagatga aacgaaaaaa gctgtgaaat accagagtca
      661 ggcccggaag aaattgataa ttatcattgt gctagtagtt gtgttgctgg gcattttagc
      721 attgattatt ggactttccg ttggntaaaa ggcgccccct c
//
