LOCUS       sequence-212259-       14222 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14222)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 14222)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHKU33.1-2.2
FEATURES             Location/Qualifiers
     source          1..14222
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             4..27
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      2186..2195
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(8145..8165)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8145..8163)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(8502..9090)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8582..8601)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9261..10052,10053..10121))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9884..9903
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10122..10226)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      10253..11595
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(11216..11263)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     11636..11654
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11949..12356
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             12357..13451
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(13698..13714)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13698..13714)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 atggattata aggatgatga tgataagact agtatgggtt ctttagctgc taccgtcggg
       61 aatggggaaa ccaacgaatc atcccttcaa caccctgcaa ctcatggtag ccataccaac
      121 aacggccata cgatcaatgg ccatacaacc aacggtcata gcctcaatgg ccatttcata
      181 aacggccaca ccgcaaacga taacggtact atcaatggct cgctaaacgg gctcggctac
      241 ccgggtcaaa ctcccatggc catctgtggc atggcttgcc gcctcccagg aggaatagca
      301 tcggctgagg agttatggga attccttttg gcaaaaaaga atgcccggac tcgtgtgcct
      361 gaatcccgct acaacgtctc cgccttccac tcccccgttt ccaaaccagg aacggtggct
      421 acagaatacg ggcattttct tgatgatagc gtcgaccttg gggcactgga cacatctgtc
      481 ttccccttga atcgcactga gctagaaaga tcggacccgc aacagcggct gatgttggag
      541 gttgccagag aggcctttga agatgccggg gtgaccgact ggagggggaa gacggtggga
      601 tgctacatcg gaaactatgg agaggactgg tctgagatgt ttgccaagga accgcagcaa
      661 tacggcttgc atcgaatcac tggtactggg gactttgtca tcgcgaatcg gctatcatac
      721 gaattcgata tcaggggtcc cagcgtcaca atccgtacgg cttgttcttc tgccctcatc
      781 ggcatcaacg aggcctgcgc ggccatcgcc cgtggagatt gcgaagccgc catcgtgggc
      841 ggccttaatc tgatcctggc gccggccatg accatttctg aaacagagca aggcgtttta
      901 tccaaggatg gctcttgcaa gaccttctct gcagatgcta acggctatgc ccgtggagaa
      961 gctgttactg ctgtcttcat taaaccattg accgacgcaa ttcgtgacgg caacccggtc
     1021 cgggctgtca tccgggccgt aacgaataac tcggacggca agacgcccgg gatatcacat
     1081 ccaagcacta gctcgcagga aaccctcatg agaagagcgt acgccatggc aggaatcacc
     1141 gactttgggg aaacagctat ggttgagtgc cacggcaccg gcacgcccat cggcgatcct
     1201 attgaggcca atgccgtggc tcgtgttttt ggagagcggg gagtttacat tggttctgtc
     1261 aagccaaatt taggccatac agagggcgcc tcgggtcttg tgtccctgat caagatggtt
     1321 aaagcccttg agcaccgcac aataccaccc cagatccggt tcacggctcc caatcccaac
     1381 atccccttta aggaggccaa actcacggtt cccctggagc caactccctg gcctcaagat
     1441 cgcctcgagc gtgtaagtct gaactccttc ggcgtgggtg gctccaacgc ccacattatc
     1501 ctcgagtctg ccgccatgca caacgccgca accgtcattc acgccacgcc ggaagaacct
     1561 caactccttt tatacacggc cagttctgcc aagtccctca ccagggtgat tgacaactac
     1621 cggcactggg ttgagaggaa cccctccaag gtcgccgacc tggcctacac tctggctagg
     1681 aagcgagatc atgggccgca cagggccttc gctattgcca acggcggcgc aattgaaacc
     1741 gtctcgccgc ccgcaagcat caagcccggc acgaagccgc ccaatatcgt catggtcttt
     1801 acaggtcagg gcgcgcagtg gccccagatg ggtcgacagc tatggcagtc caacgagacg
     1861 ttcaaggcca gcatccagca cctggatcag tgtctccaag ccatttctgg agaaaggcca
     1921 cactactcaa tccaggagga gctcaagaag ccggccaaga agagccgcgt atcttcggcc
     1981 gagatctccc agcccctgtg cacggcaatt cagatcgcct tggtcgactc cctcaaggcc
     2041 ctgggcatcg tgccgcgtgc cgttgtcggc cattccagcg gcgagattgc ggccgcctac
     2101 gcctccgggg cgctcacagc gagtgaagcc atcgtggcag ctcatcaccg tggcgccgtg
     2161 acgaataggc agaagagaaa agggtccatg gcggccattg gaatgagttg gcgagatacg
     2221 gagaagcacc tcgtgcccaa cgtcgtcatt gcctgtgaca actctcccaa aagtgtgaca
     2281 atttcgggtg acaccgacaa ggtcaaggct gtggtggccg atattcaaat gacacgtcca
     2341 gaagtgttgg ctcggctgct tcaggtagac aaagcatacc actcgtatca catggctgag
     2401 attggagacg actacgcctc gttgattagc ggccaagtga ctggaaagag tccttcgaat
     2461 gccctcttct tctctagtgt gacgggaaag ctgctggatg gagagcacaa tctcgggtcg
     2521 gcatattggc gggcaaacct cgaatccccc gtgcggttcc gtgaggccgt cacaaacctc
     2581 ctgaatcgcg aggttggcaa gcatgccatc tttatcgaga ttggccctca ctcggccctc
     2641 gcgggaccgc taagacaaat attcgccgaa acctcttcac cttgtcctta cctgtcagtc
     2701 atgatccgca accaggattg tcttggatcc tatctggctg ccgtcggcaa actccattca
     2761 ctcaatgtaa acatcaacct cgacgtcttg ttccccacgg gttcttgtct accagatctc
     2821 ccaaggtacc cgtggaaaca cgagaacaca tgctggtacg agtctcgtct gtccaaagat
     2881 tggcgcttcc gaaagttccc ctatcatgat ctccttggcc tccgagtcgt cgagagcacc
     2941 gagctcgagc cggcctggcg caacctgttt cacctatcca acacgccttg ggtccacgac
     3001 cacaagatcg gggacgatat cgttttcccc tttgccggct acattgccat ggctggcgag
     3061 gcggtgaggc agacgacggg catcaacgac gggttcagca ttcaaaatgt tcttgtcagc
     3121 acagcactgg tcctccccaa gggcaggccc acggagatga tgaccacgtt ccggccaaag
     3181 agcctcacaa cgtccctgaa cagctcctgg tgggaattca ccatggcctc gtacaacggt
     3241 gacatctgga caaaacactg cacgggcgaa gtcatggcgc tcatgtccga cccaagaccg
     3301 ggacaggatc ccgaacccct tcctcgcaag gtgaacacgg acaagtggta cagcaacatg
     3361 agcaagggcg gtcttgacct tggcccctgt ttccaaacac ttgccaccat tgagacttca
     3421 acgagtccag aggacaacag ggccattgga aatgttgtca acggcaggca gggcgatgag
     3481 gccaactacc acatccatcc caccgtgctc gacgcgacca tccagatcct aggtgccgcc
     3541 gccaccaacg gccagatgcg caagaccaag acttggcttc ctaccagcat ggacaggctc
     3601 agcgtctacc gttgctcgtc ggacatggtc accagtgtca gcgcgaggct gtcaagcaac
     3661 cattccgtag tgggaggagg ccgttgcacg gcgggcggcg tcgtcgtcgt cgaggctgag
     3721 aatatcaaaa tgtcacttgc ggccaactcc ctttcaaatg aggtccccga cacacacgcg
     3781 gccgcaaggt acgagtggag cgccgatatt gactttatga atgttggcga gctcctcctc
     3841 gcaccagcag gtcgctctga atacttgcat ctcctcgagg agctcgggca actctgcttg
     3901 ctttcctcga agcgccgact ttactcgacg tctaagccgg tggtcctcgc tcctcatatg
     3961 ctcaactatg cgtcctggat tacgtccgct tccaaggtgg tcgtcgtcaa cctagcgtct
     4021 gtcatgagca atcttgacaa cgagaacatc tcggcccgca tccacgctct ctcgaaccgt
     4081 cttgctgata ctccaatgtc gccggtcgct aatgccatcc gcgagatatg gacgaagatg
     4141 gatctcttgc tgtccggaca gacgctggaa gatattctcc cggaggagac actgtctagt
     4201 gtcttgcagt tcgtgcacaa atccattgac tatggcccat ttattcggca tctggcacac
     4261 tccaaaccca acctgcgcat cctcgagatt ggcggcgaca gaagctcatc ggtagccagt
     4321 ggcattgccg aggacctcac acgccccgat ggccaggtgc tctgttccaa gtataccttg
     4381 agctcgccag gctttgtctc cagcaaggac cagccgatgc tgctccccaa tatggaatac
     4441 accactctgg atatcggcca agatcccgcg gatcagggct ttgagggaca ccaatacgac
     4501 ctcatcatcg catccaacgt cattggggcc accaggaacc cgcagcagag cctggccaac
     4561 atcaagagac tcttgagtcc taacggccga ctgcttatgc aggagacttg cccgtcgtcc
     4621 aaatgggcca cctatgtttt cggagtcctc ccgacgtggt ggcaaggcac cgctgacggg
     4681 tctgacgaag cagccgtcct cagtcccgaa atatggcagt cgaagcttgc ggcggcaggg
     4741 ctcggcagca tcgaagcaac ggttctggat gccgagcagc ctcaccaact cactgcgacc
     4801 atagtagcaa gacatgactt gccccaggag actgccccta gacgagtcac tgttcttgtc
     4861 gaagacgaga atggagctgc cacccggcgg atgctcagcc gactagaaga tgacggttac
     4921 gccgtgacaa ggtgcaagtt gaatggctct ccgccagtcg gacaggatgt cctctcctgc
     4981 ctggacctcg acaaaccctt ctttgagaat ctcagcgagc cacgatatca gctcctcaaa
     5041 gacttcctcc tcggcctcgg cggcgccggc gttctctgga tcacggctct cagccagcgg
     5101 ggatgccgtg atccccggta cgcccaagtc ttgggactcg ctcgatgtgt ccgctccgag
     5161 atgattgccg actttgccac gtgcgaggtg gacagcttcg acaatgccca gtcgatggac
     5221 atgatccccc gggtcctggc caaattcgag aagcgcgacg acgacgccga cgacgccgta
     5281 aagtccgact ttgaatgggc cattatcgac ggccaggtac aggttgggcg cttccacaaa
     5341 ttcgtgctgc gagacgaact ccttctgtcg gaacccgctg gaatggcctc ccttgatatc
     5401 gggacgcctg gcctagtcaa cagtctgcgt tgggtgcaac agccgcgcga ggccgagctg
     5461 aagccgggcg atgtagaggt ccaggtgcac tctgtaggct tgaactttcg ggacatcctc
     5521 gtggcccttg gcatcgtgga acttccggtg cgccagtttg gattcgaagc agcaggcatc
     5581 attacacggg tcggaaagga cgtggacccc aaccagctca aggtcggcga tcgcatctgc
     5641 tgcttgaaga agcaggcctt tgggacttat attgtcactc cggactatag ttgtactcat
     5701 gtccccgaca acatcacatt tgatgaggcc gcgtccatga tcatgccata tattacggct
     5761 atccacggct tgttcactac tgcacatctt tcaaagggtc agaccgtctt gattcacagc
     5821 gcatgcggtg gcgttggtct ggccgctgtg caaatttccc aaatggtcgg cgctgaagtt
     5881 tactgttccg tcggcagaga agacaaggtc gagttcctga tgaacaactt caacatcccg
     5941 cggaatcgca ttttctactc ccgtgacaag tcctttgttg gtgacgttat gcgtgagact
     6001 ggtggccaag gcatggacgt catcctcaac tccctttccg gcgagctctt gcacgcaacg
     6061 tggacatgcg tcgccgagtt tggcaccttg gttgaaattg gaaagcgaga tctgatcggc
     6121 cgtgggaaac tcgacatgga gccctttttg aagaaccgca gctactgctg tattgacatt
     6181 gaccgcgtct ggtcgaagcc gtggattctg agaaaacaca tgctggacgc cttcgactgg
     6241 tgtgccaagg gtcttgtcac ccccgttcga cccgtcaaat ctttcccagc cagtcaaacc
     6301 caggacgcgt tccggtacat gcaaaagggg tttcacattg gacgaattgg catttccatc
     6361 tctcacaacc cagaacaccc ggatacccag tttgagactg tcaagatggc ccgacccatc
     6421 acatttgaca gatccggttc ctaccttctt gttggtgggc tcgggggtct aggccgcgcc
     6481 atttccgtct ggatggccga gcacggcgcc cgcgaattga tctacctatc acgaagcgct
     6541 ggaggggaca ccaagcacga cgtcttcgtg gccgagctca agagtatggg ctgcaacgtc
     6601 aagctcatcc agggagacgt gaccaagctc gaggacgtcg agaaggctgt caaggcggcg
     6661 acacatgccc ttcgaggcat tatccaaatg tccatggtgc tgcgtgacca aaactttacc
     6721 aaaatgacct ttgaggagtg gacgggtgcg acagccccca aggtcgaggg cacatggaac
     6781 ctacaccgtg cctccctgtc cgctggtaca gacctggact tttttgtgct cttcagctcc
     6841 gtttcgggaa tcatcggcca accaggccag gccaactatg ccagcgccaa cacctttctt
     6901 gatgcgtttg cccagtaccg caagggcttg ggcctggcag cctcggtcgt cgacgtcggg
     6961 gccgttgaag acattggcgt cattaccgag cgccaaggat taatgaacac catgaaagca
     7021 accggcttca agggcgtcac agagcaggaa ctcttggacg ccatggtctt ggcaatgttt
     7081 gcccatgctc ggccgacggg gcagggaaat agtccaggct tccactcggc ctttgtcgag
     7141 gccaacacgt ttgtcctcgg cctggactcg tcgataccgc tgagtagtcc caacaaccgc
     7201 gccgtgtgga aaagggaccg tcgcatggcg gcgtaccaca acgccgctgc caccggtgct
     7261 gaagctgccg cgtccaacga tggtcttaag tcgttcctgg ctggtgccag ggccgaccct
     7321 gctgtcctca gctcgccgga gacggctaac ctgttcgccg ttgagattgg caagaaactc
     7381 tttcacctac tgctcaagcc acaggaggac ctcaacatag ccttgccctt ggtcgacctg
     7441 ggcctcgact cgctcgtggc actcgagctc aaggcctggt ggaagcaggt cttctccttt
     7501 gacatcagtg tgctggagat gcttgcgatg ggatcccttg cggcgcttgg gaaacatgcc
     7561 gccgatcaga tgatcaagat taccgccatt gataaggacg cgtcctgagg agattgataa
     7621 gacttttcta gttgcatatc ttttatattt aaatcttatc tattagttaa ttttttgtaa
     7681 tttatcctta tatatagtct ggttattcta aaatatcatt tcagtatcta aaaattcccc
     7741 tcttttttca gttatatctt aacaggcgac agtccaaatg ttgatttatc ccagtccgat
     7801 tcatcagggt tgtgaagcat tttgtcaatg gtcgaaatca catcagtaat agtgcctctt
     7861 acttgcctca tagaatttct ttctcttaac gtcaccgttt ggtcttttat agtttcgaaa
     7921 tctatggtga taccaaatgg tgttcccaat tcatcgttac gggcgtattt tttaccaatt
     7981 gaagtattgg aatcgtcaat tttaaagtat atctctcttt tacgtaaagc ctgcgagatc
     8041 ctcttaagta tagcggggaa gccatcgtta ttcgatattg tcgtaacaaa tactttgatc
     8101 ggcgctatgc ggccgccacc gcggtggagc tccagctttt gttcccttta gtgagggtta
     8161 attgcgcgct tggcgagact caaacaagat atattttgcc cgccttacag acgaaactag
     8221 ttggaggtta tggagcatac tatcacgtgg gcggccactg gtgagttact acaccccagg
     8281 ggcaacgttg atgctcctaa aaaactctgg ctggacgcaa gccgtaacac ccgtgtcact
     8341 tcataatcgt ttgcaattca gggcttgatc tacacattac cctgttatcc ctattaatta
     8401 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     8461 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     8521 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     8581 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     8641 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     8701 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     8761 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     8821 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     8881 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     8941 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     9001 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     9061 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     9121 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     9181 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     9241 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     9301 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     9361 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     9421 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     9481 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     9541 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     9601 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     9661 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     9721 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     9781 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     9841 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     9901 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     9961 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
    10021 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
    10081 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
    10141 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
    10201 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgaacgaagc
    10261 atctgtgctt cattttgtag aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa
    10321 gaatctgagc tgcattttta cagaacagaa atgcaacgcg aaagcgctat tttaccaacg
    10381 aagaatctgt gcttcatttt tgtaaaacaa aaatgcaacg cgagagcgct aatttttcaa
    10441 acaaagaatc tgagctgcat ttttacagaa cagaaatgca acgcgagagc gctattttac
    10501 caacaaagaa tctatacttc ttttttgttc tacaaaaatg catcccgaga gcgctatttt
    10561 tctaacaaag catcttagat tacttttttt ctcctttgtg cgctctataa tgcagtctct
    10621 tgataacttt ttgcactgta ggtccgttaa ggttagaaga aggctacttt ggtgtctatt
    10681 ttctcttcca taaaaaaagc ctgactccac ttcccgcgtt tactgattac tagcgaagct
    10741 gcgggtgcat tttttcaaga taaaggcatc cccgattata ttctataccg atgtggattg
    10801 cgcatacttt gtgaacagaa agtgatagcg ttgatgattc ttcattggtc agaaaattat
    10861 gaacggtttc ttctattttg tctctatata ctacgtatag gaaatgttta cattttcgta
    10921 ttgttttcga ttcactctat gaatagttct tactacaatt tttttgtcta aagagtaata
    10981 ctagagataa acataaaaaa tgtagaggtc gagtttagat gcaagttcaa ggagcgaaag
    11041 gtggatgggt aggttatata gggatatagc acagagatat atagcaaaga gatacttttg
    11101 agcaatgttt gtggaagcgg tattcgcaat attttagtag ctcgttacag tccggtgcgt
    11161 ttttggtttt ttgaaagtgc gtcttcagag cgcttttggt tttcaaaagc gctctgaagt
    11221 tcctatactt tctagagaat aggaacttcg gaataggaac ttcaaagcgt ttccgaaaac
    11281 gagcgcttcc gaaaatgcaa cgcgagctgc gcacatacag ctcactgttc acgtcgcacc
    11341 tatatctgcg tgttgcctgt atatatatat acatgagaag aacggcatag tgcgtgttta
    11401 tgcttaaatg cgtacttata tgcgtctatt tatgtaggat gaaaggtagt ctagtacctc
    11461 ctgtgatatt atcccattcc atgcggggta tcgtatgctt ccttcagcac taccctttag
    11521 ctgttctata tgctgccact cctcaattgg attagtctca tccttcaatg ctatcatttc
    11581 ctttgatatt ggatcatact aagaaaccat tattatcatg acattaacct ataaaaatag
    11641 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgagc gcgtttcggt
    11701 gatgacggtg aaaacctcgg cgcgccatta ccctgttatc cctaatatac aacgcaaaga
    11761 agctggaaaa ttggcgaggg aatcctgttt ctgtctatcc aagaatgggc atgaggtggc
    11821 aaccgtcgtg ctagcgtaca gggtgcactt tgtaaccatt tgggacaccg gacactcgct
    11881 gttttcgaaa ttacccttta agcgcgggta ttgaaccagg cttatgccca agatcgtagc
    11941 aagcatttaa ctgtgggaat actcaggtat cgtaagatgc aagagttcga atctcttagc
    12001 aaccattatt tttttcctca acataacgag aacacacagg ggcgctatcg cacagaatca
    12061 aattcgatga ctggaaattt tttgttaatt tcagaggtcg cctgacgcat ataccttttt
    12121 caactgaaaa attgggagaa aaaggaaagg tgagagcgcc ggaaccggct tttcatatag
    12181 aatagagaag cgttcatgac taaatgcttg catcacaata cttgaagttg acaatattat
    12241 ttaaggacct attgtttttt ccaataggtg gttagcaatc gtcttacttt ctaacttttc
    12301 ttacctttta catttcagca atatatatat atatatttca aggatatacc attctaatgt
    12361 ctgcccctaa gaagatcgtc gttttgccag gtgaccacgt tggtcaagaa atcacagccg
    12421 aagccattaa ggttcttaaa gctatttctg atgttcgttc caatgtcaag ttcgatttcg
    12481 aaaatcattt aattggtggt gctgctatcg atgctacagg tgttccactt ccagatgagg
    12541 cgctggaagc ctccaagaag gctgatgccg ttttgttagg tgctgtgggt ggtcctaaat
    12601 ggggtaccgg tagtgttaga cctgaacaag gtttactaaa aatccgtaaa gaacttcaat
    12661 tgtacgccaa cttaagacca tgtaactttg catccgactc tcttttagac ttatctccaa
    12721 tcaagccaca atttgctaaa ggtactgact tcgttgttgt cagagaatta gtgggaggta
    12781 tttactttgg taagagaaag gaagacgatg gtgatggtgt cgcttgggat agtgaacaat
    12841 acaccgttcc agaagtgcaa agaatcacaa gaatggccgc tttcatggcc ctacaacatg
    12901 agccaccatt gcctatttgg tccttggata aagctaatgt tttggcctct tcaagattat
    12961 ggagaaaaac tgtggaggaa accatcaaga acgaattccc tacattgaag gttcaacatc
    13021 aattgattga ttctgccgcc atgatcctag ttaagaaccc aacccaccta aatggtatta
    13081 taatcaccag caacatgttt ggtgatatca tctccgatga agcctccgtt atcccaggtt
    13141 ccttgggttt gttgccatct gcgtccttgg cctctttgcc agacaagaac accgcatttg
    13201 gtttgtacga accatgccac ggttctgctc cagatttgcc aaagaataag gtcaacccta
    13261 tcgccactat cttgtctgct gcaatgatgt tgaaattgtc attgaacttg cctgaagaag
    13321 gtaaggccat tgaagatgca gttaaaaagg ttttggatgc aggtatcaga actggtgatt
    13381 taggtggttc caacagtacc accgaagtcg gtgatgctgt cgccgaagaa gttaagaaaa
    13441 tccttgctta aaaagattct ctttttttat gatatttgta cataaacttt ataaatgaaa
    13501 ttcataatag aaacgacacg aaattacaaa atggaatatg ttcatagggt agacgaaact
    13561 atatacgcaa tctacataca tttatcaaga aggagaaaaa ggaggatgta aaggaataca
    13621 ggtaagcaaa ttgatactaa tggctcaacg aaatgacggt atcgataagc ttgatatcga
    13681 attcctgcag cccgggggat ccactagttc tagattaatt aatatctaaa aattgcctta
    13741 tgatccgtct ctccggttac agcctgtgta actgattaat cctgcctttc taatcaccat
    13801 tctaatgttt taattaaggg attttgtctt cattaacggc tttcgctcat aaaaatgtta
    13861 tgacgttttg cccgcaggcg ggaaaccatc cacttcacga gactgatctc ctctgccgga
    13921 acaccgggca tctccaactt ataagttgga gaaataagag aatttcagat tgagagaatg
    13981 aaaaaaaaaa aaaaaaaaaa ggcagaggag agcatagaaa tggggttcac tttttggtaa
    14041 agctatagca tgcctatcac atataaatag agtgccagta gcgacttttt tcacactcga
    14101 aatactctta ctactgctct cttgttgttt ttatcacttc ttgtttcttc ttggtaaata
    14161 gaatatcaag ctacaaaaag catacaatca actatcaact attaactata tcgtaataca
    14221 ca
//
