LOCUS       sequence-21226-e         761 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Sharma N, Low SH, Misra S, Pallavi B, Weimbs T
  TITLE     Apical targeting of syntaxin 3 is essential for epithelial cell 
            polarity.
  JOURNAL   J Cell Biol. 2006 Jun 19. 173(6):937-48.
  PUBMED    16785322
REFERENCE   2  (bases 1 to 761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2006 Jun 19. 173(6):937-48."
COMMENT     Sequence Label: Syn3-27-146+TM
FEATURES             Location/Qualifiers
     source          1..761
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    40..58
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     68..92
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             272..283
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             487..516
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             526..555
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             571..588
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(611..628)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    617..728
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ctggggcttt agcgagctct cctatcagtg atagagatct ccctatcagt gatagagatc
       61 gtcgacgagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
      121 acctccatag aagacaccgg gaccgatcca gcctccggac tctagcgttt aaacttaagc
      181 ttggtaccga gctcggatcc actagtccag tgtggtggaa ttcgatatgg ggatcattga
      241 ctcacagatt tccaagcaag ccctcagtga gattgaggga cgacacaagg acattgtgag
      301 gctggagagc agcatcaagg agcttcacga catgtttatg gacatcgcca tgctggtgga
      361 gaatcagggt gagatgttag ataacataga gttgaatgtc atgcacacag tggaccacgt
      421 ggagaaggca cgagatgaaa cgaaaaaagc tgtgaaatac cagagtcagg cccggaagaa
      481 actcgagaac aaaaactcat ctcagaagag gatctggggc ccttcgaaca aaaactcatc
      541 tcagaagagg atctgaatat gcataccggt catcatcacc atcaccattg agtttaaacc
      601 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
      661 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
      721 attgcatcgc attgtctgag taggtgtcat tctattctgg g
//
