LOCUS       sequence-212264-       12301 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus for constitutive expression of ABE7.10 (not codon 
            optimized).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12301)
  TITLE     Dow Lab Unpublished Base Editors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12301)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti-ABE7.10-P2A-Puro
FEATURES             Location/Qualifiers
     source          1..12301
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1723..1840
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1909..2120
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             join(2146..2646,2647..2742,2743..3240,3241..3336)
                     /codon_start=1
                     /product="adenine base editor comprising wild-type E. coli 
                     TadA fused to the evolved TadA*(7.10) mutant (Gaudelli et 
                     al., 2017)"
                     /label=ABE(7.10)
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEGW
                     NRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRVVF
                     GARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQKKAQ
                     SSTDSGGSSGGSSGSETPGTSESATPESSGGSSGGSSEVEFSHEYWMRHALTLAKRARD
                     EREVPVGAVLVLNNRVIGEGWNRAIGLHDPTAHAEIMALRQGGLVMQNYRLIDATLYVT
                     FEPCVMCAGAMIHSRIGRVVFGVRNAKTGAAGSLMDVLHYPGMNHRVEITEGILADECA
                     ALLCYFFRMPRQVFNAQKKAQSSTDSGGSSGGSSGSETPGTSESATPESSGGSSGGS"
     CDS             join(3337..6735,6736..6738,6739..7335,7336,7337..7339,
                     7340..7341,7342..7344,7345..7437)
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 endonuclease with the
                     D1135V/R1335Q/T1337R mutations"
                     /label=Cas9 VQR
                     /note="has an altered recognition specificity for the 
                     protospacer adjacent motif (PAM) (Kleinstiver et al., 
                     2015)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(3337..3360,3361..3363,3364..7437)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7450..7470
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7486..7542
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7543..8142
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7543..7562)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8039..8059
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8158..8746
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8211..8231)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8629..8640)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8818..9051
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9129..9250
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9166..9185)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9220..9239
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9290..9425
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9445..9464)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9446..9464)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9474..9491)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9474..9490)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9483..9505)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9632..10087
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9719..9738)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9929..9950
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10113..10217
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10218..10286,10287..11078)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10436..10455)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11249..11837
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11738..11757
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11991..12008
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12125..12146
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12161..12166,12167..12184,12185..12191)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12199..12215
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12204..12226
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12223..12239
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12223..12239
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12258..12278
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12260..12278
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccagaccac aattttaaaa gaaaaggggg
     1741 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     1801 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     1861 cagagatcca ctttggcgcc ggctcgagtg gctccggtgc ccgtcagtgg gcagagcgca
     1921 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     1981 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     2041 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     2101 ggtttgccgc cagaacacag gtgtcgtgac gcgggatccg ccaccatgtc cgaagtcgag
     2161 ttttcccatg agtactggat gagacacgca ttgactctcg caaagagggc ttgggatgaa
     2221 cgcgaggtgc ccgtgggggc agtactcgtg cataacaatc gcgtaatcgg cgaaggttgg
     2281 aataggccga tcggacgcca cgaccccact gcacatgcgg aaatcatggc ccttcgacag
     2341 ggagggcttg tgatgcagaa ttatcgactt atcgatgcga cgctgtacgt cacgcttgaa
     2401 ccttgcgtaa tgtgcgcggg agctatgatt cactcccgca ttggacgagt tgtattcggt
     2461 gcccgcgacg ccaagacggg tgccgcaggt tcactgatgg acgtgctgca tcacccaggc
     2521 atgaaccacc gggtagaaat cacagaaggc atattggcgg acgaatgtgc ggcgctgttg
     2581 tccgactttt ttcgcatgcg gaggcaggag atcaaggccc agaaaaaagc acaatcctct
     2641 actgactctg gtggttcttc tggtggttct agcggcagcg agactcccgg gacctcagag
     2701 tccgccacac ccgaaagttc tggtggttct tctggtggtt cttccgaagt cgagttttcc
     2761 catgagtact ggatgagaca cgcattgact ctcgcaaaga gggctcgaga tgaacgcgag
     2821 gtgcccgtgg gggcagtact cgtgctcaac aatcgcgtaa tcggcgaagg ttggaatagg
     2881 gcaatcggac tccacgaccc cactgcacat gcggaaatca tggcccttcg acagggaggg
     2941 cttgtgatgc agaattatcg acttatcgat gcgacgctgt acgtcacgtt tgaaccttgc
     3001 gtaatgtgcg cgggagctat gattcactcc cgcattggac gagttgtatt cggtgttcgc
     3061 aacgccaaga cgggtgccgc aggttcactg atggacgtgc tgcattaccc aggcatgaac
     3121 caccgggtag aaatcacaga aggcatattg gcggacgaat gtgcggcgct gttgtgttac
     3181 ttttttcgca tgcccaggca ggtctttaac gcccagaaaa aagcacaatc ctctactgac
     3241 tctggtggtt cttctggtgg ttctagcggc agcgagactc ccgggacctc agagtccgcc
     3301 acacccgaaa gttctggtgg ttcttctggt ggttctgata aaaagtattc tattggttta
     3361 gccatcggca ctaattccgt tggatgggct gtcataaccg atgaatacaa agtaccttca
     3421 aagaaattta aggtgttggg gaacacagac cgtcattcga ttaaaaagaa tcttatcggt
     3481 gccctcctat tcgatagtgg cgaaacggca gaggcgactc gcctgaaacg aaccgctcgg
     3541 agaaggtata cacgtcgcaa gaaccgaata tgttacttac aagaaatttt tagcaatgag
     3601 atggccaaag ttgacgattc tttctttcac cgtttggaag agtccttcct tgtcgaagag
     3661 gacaagaaac atgaacggca ccccatcttt ggaaacatag tagatgaggt ggcatatcat
     3721 gaaaagtacc caacgattta tcacctcaga aaaaagctag ttgactcaac tgataaagcg
     3781 gacctgaggt taatctactt ggctcttgcc catatgataa agttccgtgg gcactttctc
     3841 attgagggtg atctaaatcc ggacaactcg gatgtcgaca aactgttcat ccagttagta
     3901 caaacctata atcagttgtt tgaagagaac cctataaatg caagtggcgt ggatgcgaag
     3961 gctattctta gcgcccgcct ctctaaatcc cgacggctag aaaacctgat cgcacaatta
     4021 cccggagaga agaaaaatgg gttgttcggt aaccttatag cgctctcact aggcctgaca
     4081 ccaaatttta agtcgaactt cgacttagct gaagatgcca aattgcagct tagtaaggac
     4141 acgtacgatg acgatctcga caatctactg gcacaaattg gagatcagta tgcggactta
     4201 tttttggctg ccaaaaacct tagcgatgca atcctcctat ctgacatact gagagttaat
     4261 actgagatta ccaaggcgcc gttatccgct tcaatgatca aaaggtacga tgaacatcac
     4321 caagacttga cacttctcaa ggccctagtc cgtcagcaac tgcctgagaa atataaggaa
     4381 atattctttg atcagtcgaa aaacgggtac gcaggttata ttgacggcgg agcgagtcaa
     4441 gaggaattct acaagtttat caaacccata ttagagaaga tggatgggac ggaagagttg
     4501 cttgtaaaac tcaatcgcga agatctactg cgaaagcagc ggactttcga caacggtagc
     4561 attccacatc aaatccactt aggcgaattg catgctatac ttagaaggca ggaggatttt
     4621 tatccgttcc tcaaagacaa tcgtgaaaag attgagaaaa tcctaacctt tcgcatacct
     4681 tactatgtgg gacccctggc ccgagggaac tctcggttcg catggatgac aagaaagtcc
     4741 gaagaaacga ttactccatg gaattttgag gaagttgtcg ataaaggtgc gtcagctcaa
     4801 tcgttcatcg agaggatgac caactttgac aagaatttac cgaacgaaaa agtattgcct
     4861 aagcacagtt tactttacga gtatttcaca gtgtacaatg aactcacgaa agttaagtat
     4921 gtcactgagg gcatgcgtaa acccgccttt ctaagcggag aacagaagaa agcaatagta
     4981 gatctgttat tcaagaccaa ccgcaaagtg acagttaagc aattgaaaga ggactacttt
     5041 aagaaaattg aatgcttcga ttctgtcgag atctccgggg tagaagatcg atttaatgcg
     5101 tcacttggta cgtatcatga cctcctaaag ataattaaag ataaggactt cctggataac
     5161 gaagagaatg aagatatctt agaagatata gtgttgactc ttaccctctt tgaagatcgg
     5221 gaaatgattg aggaaagact aaaaacatac gctcacctgt tcgacgataa ggttatgaaa
     5281 cagttaaaga ggcgtcgcta tacgggctgg ggacgattgt cgcggaaact tatcaacggg
     5341 ataagagaca agcaaagtgg taaaactatt ctcgattttc taaagagcga cggcttcgcc
     5401 aataggaact ttatgcagct gatccatgat gactctttaa ccttcaaaga ggatatacaa
     5461 aaggcacagg tttccggaca aggggactca ttgcacgaac atattgcgaa tcttgctggt
     5521 tcgccagcca tcaaaaaggg catactccag acagtcaaag tagtggatga gctagttaag
     5581 gtcatgggac gtcacaaacc ggaaaacatt gtaatcgaga tggcacgcga aaatcaaacg
     5641 actcagaagg ggcaaaaaaa cagtcgagag cggatgaaga gaatagaaga gggtattaaa
     5701 gaactgggca gccagatctt aaaggagcat cctgtggaaa atacccaatt gcagaacgag
     5761 aaactttacc tctattacct acaaaatgga agggacatgt atgttgatca ggaactggac
     5821 ataaaccgtt tatctgatta cgacgtcgat cacattgtac cccaatcctt tttgaaggac
     5881 gattcaatcg acaataaagt gcttacacgc tcggataaga accgagggaa aagtgacaat
     5941 gttccaagcg aggaagtcgt aaagaaaatg aagaactatt ggcggcagct cctaaatgcg
     6001 aaactgataa cgcaaagaaa gttcgataac ttaactaaag ctgagagggg tggcttgtct
     6061 gaacttgaca aggccggatt tattaaacgt cagctcgtgg aaacccgcca aatcacaaag
     6121 catgttgcac agatactaga ttcccgaatg aatacgaaat acgacgagaa cgataagctg
     6181 attcgggaag tcaaagtaat cactttaaag tcaaaattgg tgtcggactt cagaaaggat
     6241 tttcaattct ataaagttag ggagataaat aactaccacc atgcgcacga cgcttatctt
     6301 aatgccgtcg tagggaccgc actcattaag aaatacccga agctagaaag tgagtttgtg
     6361 tatggtgatt acaaagttta tgacgtccgt aagatgatcg cgaaaagcga acaggagata
     6421 ggcaaggcta cagccaaata cttcttttat tctaacatta tgaatttctt taagacggaa
     6481 atcactctgg caaacggaga gatacgcaaa cgacctttaa ttgaaaccaa tggggagaca
     6541 ggtgaaatcg tatgggataa gggccgggac ttcgcgacgg tgagaaaagt tttgtccatg
     6601 ccccaagtca acatagtaaa gaaaactgag gtgcagaccg gagggttttc aaaggaatcg
     6661 attcttccaa aaaggaatag tgataagctc atcgctcgta aaaaggactg ggacccgaaa
     6721 aagtacggtg gcttcgatag ccctacagtt gcctattctg tcctagtagt ggcaaaagtt
     6781 gagaagggaa aatccaagaa actgaagtca gtcaaagaat tattggggat aacgattatg
     6841 gagcgctcgt cttttgaaaa gaaccccatc gacttccttg aggcgaaagg ttacaaggaa
     6901 gtaaaaaagg atctcataat taaactacca aagtatagtc tgtttgagtt agaaaatggc
     6961 cgaaaacgga tgttggctag cgccggagag cttcaaaagg ggaacgaact cgcactaccg
     7021 tctaaatacg tgaatttcct gtatttagcg tcccattacg agaagttgaa aggttcacct
     7081 gaagataacg aacagaagca actttttgtt gagcagcaca aacattatct cgacgaaatc
     7141 atagagcaaa tttcggaatt cagtaagaga gtcatcctag ctgatgccaa tctggacaaa
     7201 gtattaagcg catacaacaa gcacagggat aaacccatac gtgagcaggc ggaaaatatt
     7261 atccatttgt ttactcttac caacctcggc gctccagccg cattcaagta ttttgacaca
     7321 acgatagatc gcaaacgata cacttctacc aaggaggtgc tagacgcgac actgattcac
     7381 caatccatca cgggattata tgaaactcgg atagatttgt cacagcttgg gggtgactct
     7441 ggtggttctc ccaagaagaa gaggaaagtc atgcatggca gcggcgccac caacttcagc
     7501 ctgctgaagc aggccggcga cgtggaggag aaccccggcc ccatgaccga gtacaagccc
     7561 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     7621 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     7681 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     7741 gcggacgacg gcgcggccgt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     7801 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     7861 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     7921 accgtcggag tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     7981 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     8041 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     8101 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaacgcgtta agtcgacaat
     8161 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     8221 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     8281 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     8341 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     8401 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     8461 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     8521 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     8581 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     8641 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     8701 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcgtcg actttaagac
     8761 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     8821 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     8881 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     8941 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     9001 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtacgtata
     9061 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     9121 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     9181 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     9241 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     9301 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     9361 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     9421 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     9481 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     9541 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     9601 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     9661 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     9721 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     9781 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     9841 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     9901 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     9961 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
    10021 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
    10081 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
    10141 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
    10201 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
    10261 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
    10321 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
    10381 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
    10441 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
    10501 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
    10561 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
    10621 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
    10681 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
    10741 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
    10801 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
    10861 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
    10921 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
    10981 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
    11041 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
    11101 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
    11161 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
    11221 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
    11281 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
    11341 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
    11401 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
    11461 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
    11521 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
    11581 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
    11641 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
    11701 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
    11761 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
    11821 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
    11881 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
    11941 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
    12001 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
    12061 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
    12121 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
    12181 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
    12241 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag
    12301 c
//
