LOCUS       sequence-212269-        5696 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5696)
  TITLE     SGC Oxford April 2018 plasmids
REFERENCE   2  (bases 1 to 5696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: LIMK2 (LIMK2A-c052)
FEATURES             Location/Qualifiers
     source          1..5696
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..92
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     17..34
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             154..171
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             196..216
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     polyA_signal    1302..1436
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1339..1358)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1393..1412
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  1465..1630
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      1814..2268
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1901..1920)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2110..2131
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2294..2398
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2399..2467,2468..3259)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2617..2636)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3430..4018
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3919..3938
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4172..4189
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  4323..4547
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(4614..5147)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(5317..5322,5323..5339,5340..5345))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 atcatggaga taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc
       61 gtaacagttt tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca
      121 tcgggcgcgg atctcggtcc gaaaaccatg ggccaccatc atcatcatca ttcttctggt
      181 gtagatctgg gtaccgagaa cctgtacttc caatccatgg acctaatcca tggggaggtc
      241 ctggggaagg gcttctttgg gcaggctatc aaggtgacac acaaagccac gggcaaagtg
      301 atggtcatga aagagttaat tcgatgtgat gaggagaccc agaaaacttt tctgactgag
      361 gtgaaagtga tgcgcagcct ggaccacccc aatgtgctca agttcattgg tgtgctgtac
      421 aaggataaga agctgaacct gctgacagag tacattgagg ggggcacact gaaggacttt
      481 ctgcgcagta tggatccgtt cccctggcag cagaaggtca ggtttgccaa aggaatcgcc
      541 tccggaatgg cctatttgca ctctatgtgc atcatccacc gggatctgaa ctcgcacaac
      601 tgcctcatca agttggacaa gactgtggtg gtggcagact ttgggctgtc acggctcata
      661 gtggaagaga ggaaaagggc ccccatggag aaggccacca ccaagaaacg caccttgcgc
      721 aagaacgacc gcaagaagcg ctacacggtg gtgggaaacc cctactggat ggcccctgag
      781 atgctgaacg gaaagagcta tgatgagacg gtggatatct tctcctttgg gatcgttctc
      841 tgtgagatca ttgggcaggt gtatgcagat cctgactgcc ttccccgaac actggacttt
      901 ggcctcaacg tgaagctttt ctgggagaag tttgttccca cagattgtcc cccggccttc
      961 ttcccgctgg ccgccatctg ctgcagactg gagcctgaga gcagaccagc attctcgaaa
     1021 ttggaggact cctttgaggc cctctccctg tacctggggg agctgggcat cccgctgcct
     1081 gcagagctgg aggagttgga ccacactgtg agcatgcagt acggcctgtg acagtaaagg
     1141 tggatacgga tccgaattcg agctccgtcg acaagcttgt cgagaagtac tagaggatca
     1201 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
     1261 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
     1321 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     1381 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatctgat
     1441 cactgatatc gcctaggaga tccgaaccag ataagtgaaa tctagttcca aactattttg
     1501 tcatttttaa ttttcgtatt agcttacgac gctacaccca gttcccatct attttgtcac
     1561 tcttccctaa ataatcctta aaaactccat ttccacccct cccagttccc aactattttg
     1621 tccgcccaca gcggggcatt tttcttcctg ttatgttttt aatcaaacat cctgccaact
     1681 ccatgtgaca aaccgtcatc ttcggctact ttttctctgt cacagaatga aaatttttct
     1741 gtcatctctt cgttattaat gtttgtaatt gactgaatat caacgcttat ttgcagcctg
     1801 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     1861 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     1921 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     1981 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     2041 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     2101 ttcttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     2161 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     2221 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
     2281 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     2341 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     2401 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     2461 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     2521 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     2581 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     2641 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     2701 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     2761 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     2821 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     2881 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     2941 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     3001 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     3061 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     3121 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     3181 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     3241 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     3301 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     3361 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     3421 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     3481 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     3541 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     3601 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     3661 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     3721 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     3781 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     3841 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     3901 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     3961 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     4021 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     4081 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     4141 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     4201 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatagacca
     4261 gccgcgtaac ctggcaaaat cggttacggt tgagtaataa atggatgccc tgcgtaagcg
     4321 ggtgtgggcg gacaataaag tcttaaactg aacaaaatag atctaaacta tgacaataaa
     4381 gtcttaaact agacagaata gttgtaaact gaaatcagtc cagttatgct gtgaaaaagc
     4441 atactggact tttgttatgg ctaaagcaaa ctcttcattt tctgaagtgc aaattgcccg
     4501 tcgtattaaa gaggggcgtg gccaagggca tggtaaagac tatattcgcg gcgttgtgac
     4561 aatttaccga acaactccgc ggccgggaag ccgatctcgg cttgaacgaa ttgttaggtg
     4621 gcggtacttg ggtcgatatc aaagtgcatc acttcttccc gtatgcccaa ctttgtatag
     4681 agagccactg cgggatcgtc accgtaatct gcttgcacgt agatcacata agcaccaagc
     4741 gcgttggcct catgcttgag cagattgatg agcgcggtgg caatgccctg cctccggtgc
     4801 tcgccggaga ctgcgagatc atagatatag atctcactac gcggctgctc aaacttgggc
     4861 agaacgtaag ccgcgagagc gccaacaacc gcttcttggt cgaaggcagc aagcgcgatg
     4921 aatgtcttac tacggagcaa gttcccgagg taatcggagt ccggctgatg ttgggagtag
     4981 gtggctacgt ctccgaactc acgaccgaaa agatcaagag cagcccgcat ggatttgact
     5041 tggtcagggc cgagcctaca tgtgcgaatg atgcccatac ttgagccacc taactttgtt
     5101 ttagggcgac tgccctgctg cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc
     5161 acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtacaaa aaaacagtca
     5221 taacaagcca tgaaaaccgc cactgcgccg ttaccaccgc tgcgttcggt caaggttctg
     5281 gaccagttgc gtgagcgcat acgctacttg cattacagtt tacgaaccga acaggcttat
     5341 gtcaactggg ttcgtgcctt catccgtttc cacggtgtgc gtcacccggc aaccttgggc
     5401 agcagcgaag tcgaggcatt tctgtcctgg ctggcgaacg agcgcaaggt ttcggtctcc
     5461 acgcatcgtc aggcattggc ggccttgctg ttcttctacg gcaaggtgct gtgcacggat
     5521 ctgccctggc ttcaggagat cggaagacct cggccgtcgc ggcgcttgcc ggtggtgctg
     5581 accccggatg aagtggttcg catcctcggt tttctggaag gcgagcatcg tttgttcgcc
     5641 caggactcta gctatagttc tagtggttgg ctacgtatac tccggaatat taatag
//
