LOCUS       sequence-21227-e         617 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Oberdoerffer P, Kanellopoulou C, Heissmeyer V, Paeper C, Borowski C,
            Aifantis I, Rao A, Rajewsky K
  TITLE     Efficiency of RNA interference in the mouse hematopoietic system 
            varies between cell types and developmental stages.
  JOURNAL   Mol Cell Biol. 2005 May . 25(10):3896-905.
  PUBMED    15870264
REFERENCE   2  (bases 1 to 617)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2005 May . 25(10):3896-905."
COMMENT     Sequence Label: pU6-STOP-shA1
FEATURES             Location/Qualifiers
     source          1..617
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     257..277
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     protein_bind    complement(310..343)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tctgcgaagc ttgcggccgc tctagatccg acgccgccat ctctaggccc gcgccggccc
       61 cctcgcacag acttgtggga gaagctcggc tactcccctg ccccggttaa tttgcatata
      121 atatttccta gtaactatag aggcttaatg tgcgataaaa gacagataat ctgttctttt
      181 taatactagc tacattttac atgataggct tggatttcta taagagatac aaatactaaa
      241 ttattatttt aaaaaacagc acaaaaggaa actcacccta actgtaaagt aattgtgtgt
      301 tttgagacta taacttcgta tagcatacat tatacgaagt tattacgttt ttgcgatttt
      361 tgaattcgtt cctcagagga actgacaagc accctaacat cctattggag gctcactcac
      421 gttttttcta ttttgtttct tgacagcaga gctcgttgct cactgtatag ctcaggttgg
      481 cctgacactg atgaggttct ccagtgactg cctctaccta cctactggga tgacagaggt
      541 gtaccaccaa gccacgcccg ggggatccat aacttcgtat agcatacatt ataccgaagg
      601 aaatgctctt tctcctc
//
