LOCUS       sequence-212278-        5801 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5801)
  TITLE     SGC Oxford April 2018 plasmids
REFERENCE   2  (bases 1 to 5801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ACVR1 (ACVR1Z-c041)
FEATURES             Location/Qualifiers
     source          1..5801
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..92
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     17..34
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             154..171
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             196..216
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     polyA_signal    1407..1541
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1444..1463)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1498..1517
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  1570..1735
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      1919..2373
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2006..2025)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2215..2236
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2399..2503
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2504..2572,2573..3364)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2722..2741)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3535..4123
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4024..4043
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4277..4294
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  4428..4652
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(4719..5252)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(5422..5427,5428..5444,5445..5450))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 atcatggaga taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc
       61 gtaacagttt tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca
      121 tcgggcgcgg atctcggtcc gaaaaccatg ggccaccatc atcatcatca ttcttctggt
      181 gtagatctgg gtaccgagaa cctgtacttc caatccatga ccaccaatgt tggagacagc
      241 actttagcag atttattgga tcattcgtgt acatcaggaa gtggctctgg tcttcctttt
      301 ctggtacaaa gaacagtggc tcgcgagatt acactgttgg agtgtgtcgg gaaaggcagg
      361 tatggtgagg tgtggagggg cagctggcaa ggggaaaatg ttgccgtgaa gatcttctcc
      421 tcccgtgatg agaagtcatg gttcagggaa acggaattgt acaacactgt gatgctgagg
      481 catgaaaata tcttaggttt cattgcttca gacatgacat caagacactc cagtacccag
      541 ctgtggttaa ttacacatta tcatgaaatg ggatcgttgt acgactatct tcagcttact
      601 actctggata cagttagctg ccttcgaata gtgctgtcca tagctagtgg tcttgcacat
      661 ttgcacatag agatatttgg gacccaaggg aaaccagcca ttgcccatcg agatttaaag
      721 agcaaaaata ttctggttaa gaagaatgga cagtgttgca tagcagattt gggcctggca
      781 gtcatgcatt cccagagcac caatcagctt gatgtgggga acaatccccg tgtgggcacc
      841 aagcgctaca tggcccccga agttctagat gaaaccatcc aggtggattg tttcgattct
      901 tataaaaggg tcgatatttg ggcctttgga cttgttttgt gggaagtggc caggcggatg
      961 gtgagcaatg gtatagtgga ggattacaag ccaccgttct acgatgtggt tcccaatgac
     1021 ccaagttttg aagatatgag gaaggtagtc tgtgtggatc aacaaaggcc aaacataccc
     1081 aacagatggt tctcagaccc gacattaacc tctctggcca agctaatgaa agaatgctgg
     1141 tatcaaaatc catccgcaag actcacagca ctgcgtatca aaaagacttt gaccaaaatt
     1201 gataattccc tcgacaaatt gaaaactgac tgttgacagt aaaggtggat acggatccga
     1261 attcgagctc cgtcgacaag cttgtcgaga agtactagag gatcataatc agccatacca
     1321 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     1381 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     1441 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     1501 gtttgtccaa actcatcaat gtatcttatc atgtctggat ctgatcactg atatcgccta
     1561 ggagatccga accagataag tgaaatctag ttccaaacta ttttgtcatt tttaattttc
     1621 gtattagctt acgacgctac acccagttcc catctatttt gtcactcttc cctaaataat
     1681 ccttaaaaac tccatttcca cccctcccag ttcccaacta ttttgtccgc ccacagcggg
     1741 gcatttttct tcctgttatg tttttaatca aacatcctgc caactccatg tgacaaaccg
     1801 tcatcttcgg ctactttttc tctgtcacag aatgaaaatt tttctgtcat ctcttcgtta
     1861 ttaatgtttg taattgactg aatatcaacg cttatttgca gcctgaatgg cgaatgggac
     1921 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     1981 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     2041 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     2101 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     2161 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt aatagtggac
     2221 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     2281 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     2341 cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg
     2401 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     2461 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     2521 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     2581 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     2641 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     2701 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     2761 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     2821 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     2881 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     2941 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     3001 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     3061 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     3121 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     3181 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     3241 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     3301 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     3361 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     3421 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     3481 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     3541 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     3601 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     3661 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     3721 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     3781 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     3841 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     3901 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     3961 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     4021 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     4081 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     4141 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     4201 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     4261 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta
     4321 ttttctcctt acgcatctgt gcggtatttc acaccgcata gaccagccgc gtaacctggc
     4381 aaaatcggtt acggttgagt aataaatgga tgccctgcgt aagcgggtgt gggcggacaa
     4441 taaagtctta aactgaacaa aatagatcta aactatgaca ataaagtctt aaactagaca
     4501 gaatagttgt aaactgaaat cagtccagtt atgctgtgaa aaagcatact ggacttttgt
     4561 tatggctaaa gcaaactctt cattttctga agtgcaaatt gcccgtcgta ttaaagaggg
     4621 gcgtggccaa gggcatggta aagactatat tcgcggcgtt gtgacaattt accgaacaac
     4681 tccgcggccg ggaagccgat ctcggcttga acgaattgtt aggtggcggt acttgggtcg
     4741 atatcaaagt gcatcacttc ttcccgtatg cccaactttg tatagagagc cactgcggga
     4801 tcgtcaccgt aatctgcttg cacgtagatc acataagcac caagcgcgtt ggcctcatgc
     4861 ttgagcagat tgatgagcgc ggtggcaatg ccctgcctcc ggtgctcgcc ggagactgcg
     4921 agatcataga tatagatctc actacgcggc tgctcaaact tgggcagaac gtaagccgcg
     4981 agagcgccaa caaccgcttc ttggtcgaag gcagcaagcg cgatgaatgt cttactacgg
     5041 agcaagttcc cgaggtaatc ggagtccggc tgatgttggg agtaggtggc tacgtctccg
     5101 aactcacgac cgaaaagatc aagagcagcc cgcatggatt tgacttggtc agggccgagc
     5161 ctacatgtgc gaatgatgcc catacttgag ccacctaact ttgttttagg gcgactgccc
     5221 tgctgcgtaa catcgttgct gctccataac atcaaacatc gacccacggc gtaacgcgct
     5281 tgctgcttgg atgcccgagg catagactgt acaaaaaaac agtcataaca agccatgaaa
     5341 accgccactg cgccgttacc accgctgcgt tcggtcaagg ttctggacca gttgcgtgag
     5401 cgcatacgct acttgcatta cagtttacga accgaacagg cttatgtcaa ctgggttcgt
     5461 gccttcatcc gtttccacgg tgtgcgtcac ccggcaacct tgggcagcag cgaagtcgag
     5521 gcatttctgt cctggctggc gaacgagcgc aaggtttcgg tctccacgca tcgtcaggca
     5581 ttggcggcct tgctgttctt ctacggcaag gtgctgtgca cggatctgcc ctggcttcag
     5641 gagatcggaa gacctcggcc gtcgcggcgc ttgccggtgg tgctgacccc ggatgaagtg
     5701 gttcgcatcc tcggttttct ggaaggcgag catcgtttgt tcgcccagga ctctagctat
     5761 agttctagtg gttggctacg tatactccgg aatattaata g
//
