LOCUS       sequence-212298-        6290 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pNIFV is a vector plasmid with mutations in the cis-acting region, 
            to prevent correction of the integrase by recombination when used 
            with pCiPS-NIFV to produce Non-Integrating Foamy Virus vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6290)
  AUTHORS   Deyle DR, Li Y, Olson EM, Russell DW
  TITLE     Nonintegrating foamy virus vectors.
  JOURNAL   J Virol. 2010 Sep;84(18):9341-9. doi: 10.1128/JVI.00394-10. Epub 
            2010 Jun 30.
  PUBMED    20592072
REFERENCE   2  (bases 1 to 6290)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JVI.00394-10"; journalName: "J Virol"; date: "2010-09"; 
            volume: "84"; issue: "18"; pages: "9341-9"
COMMENT     Sequence Label: pNIFV
FEATURES             Location/Qualifiers
     source          1..6290
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        79..458
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        537..588
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     539..559
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(4286..4303)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4457..5045)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4537..4556)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5216..6007,6008..6076))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5839..5858
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6077..6181)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6249..6267
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ttggcccatt gcatacgttg tatccatatc ataatatgta catttatatt ggctcatgtc
       61 caacattacc gccatgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
      121 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
      181 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
      241 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
      301 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
      361 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
      421 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
      481 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
      541 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagcttc tagattgtac
      601 gggagctctc ttcactactc gctgcgtcga gagtgtacga gactctccag gtttggtaag
      661 aaatatttta tattgttata atgttactat gatccattaa cactctgctt atagattgta
      721 agggtgattg caatgctttc tgcataaaac tttggttttc ttgttaatca ataaaccgac
      781 ttgattcgag aacctactca tatattattg tctcttttat actttattaa gtaaaaggat
      841 ttgtatatta gccttgctaa gggagacatc tagtgatata agtgtgaact acacttatct
      901 taaatgatgt aactccttag gataatcaat atacaaaatt ccatgacaat tggcgcccaa
      961 cgtggggctc gaatataagt cgggtttatt tgtaaattat ccctagggac ctccgagcat
     1021 agcgggaggc atataaaagc caatagacaa tggctagcag gaagtaatgt tgaagaatat
     1081 gaacttgatg ttgaagctct ggttgtaatt ttaagagata gaaatatacc aagaaatcct
     1141 ttacatggag aagttatagg tcttcgcctt actgaaggat ggtggggaca aattgagaga
     1201 tttcagatgg tacgttgatc taaggctatg gatttggcca tgggacaaga aatattagtt
     1261 tatagtccca ttgtatctat gactaaaata caaaaaactc cactaccaga aagaaaagct
     1321 ttacccatta gatggataac atggatgact tatttagaag atccaagaat ccaatttcat
     1381 tatgataaaa ccttaccaga acttaagcat attccagatg tatatacatc tagtcagtct
     1441 cctgttaaac atccttctca atatgaagga gtgttttata ctgatggctc ggccatcaaa
     1501 agtcctgatc ctacaaaaag caataatgct ggcatgggaa tagtacatgc cacatacaaa
     1561 cctgaatatc aagttttgaa tcaatggtca ataccactag gtaatcatac tgctcagatg
     1621 gctgaaatag ctgcagttga atttgcctgt aaaaaagctt taaaaatacc tggtcctgta
     1681 ttagttataa ctgatagttt ctatgtagca gaaagtgcta ataaagaatt accatactgg
     1741 aaatctaatg ggtttgttaa taataagaaa aagcctctta aacatatctc caaatggaag
     1801 tctattgctg agtgtttatc tatgaaacca gacattacta ttcaacatga aaaagggcat
     1861 cagcctacaa ataccagtat tcatactgaa ggcaatgccc tagcagataa gcttgccacc
     1921 caaggaagtt atgtagttaa ttgtaatacc aaaaaaccaa acctggatgc agagttggat
     1981 caattattac agggtcatta tataaaagga tatcccaaac aatatacata ttttttagaa
     2041 gatggcaaag taaaagtttc cagacctgaa ggggttaaaa ttattccccc tcagtcagac
     2101 agacaaaaaa ttgtgcttca agcccacaat ttggctcaca ccggacgtga agccactctt
     2161 ttaaaaattg ccaaccttta ttggtggcca aatatgagaa aggatgtggt taaacaacta
     2221 ggacgctgtc aacagtgttt aatcacaaat gcttccaaca aagcctctgg tcctattcta
     2281 agaccagata ggcctcaaaa accttttgat aaattcttta ttgtctatat tggacctttg
     2341 ccaccttcac agggatacct atatgtatta gtagttgttg atggaatgac aggattcact
     2401 tggttatacc ccactaaggc tccttctact agcgcaactg ttaaatctct caatgtactc
     2461 actagtattg caattccaaa ggtgattcac tctgctcaag gtgcagcatt cacttcttca
     2521 acctttgctg aatgggcaaa ggaaagaggt atacatttgg aattcagtac tccttatcac
     2581 ccccaaagta gtggtaaggt ggaaaggaaa aatagtgata taaaacgact tttaactaaa
     2641 ctgctagtag gaagacccac aaagtggtat gacctattgc ctgttgtaca acttgcttta
     2701 aacaacacct atagccctgt attaaaatat actccacatc aactcttatt tggtatagat
     2761 tcaaatactc catttgcaaa tcaagataca cttgacttga ccagagaaga agaactttct
     2821 cttttacagg aaattcgtac ttctttatac catccatcca cccctccaac ctcctctcgt
     2881 tcctggtctc ctgttgttgg ccaattggtc caggagaggg tggctaggcc tgcttctttg
     2941 agacctcgtt ggcataaacc gtctactgta cttaaggtgt tgaatccaag gactgttgtt
     3001 attttggacc atcttggcaa caacagaact gtaagtatag ataatttaaa acctacttct
     3061 catcagaatg gcaccaccaa tgacactgca acaatggatc atttggaaca aaatgaataa
     3121 agcgcatgag gcacttcaga atacaacaac tgtgactgaa cagcagaagg aacaaattat
     3181 actggacatt caaaatgaag aagtacaacc aactaggaga gataaattta gatatctgct
     3241 ttatacttgt tgtgctacta gctcaagagt attggcctgg atatttttag tttgtatatt
     3301 gttaatcatt gttttggttt catgctttgt gactatatcc agaatacaat ggaataagga
     3361 tattcaggta ttaggacctg taatagactg gaatgttact caaagagctg tttatcaacc
     3421 cttacagact agaaggattg cacgttccct tagaatgcag catcctgttc caaaatatgt
     3481 ggaggtaaat atgactagta ttccacaagg tgtatactat gaaccccatc cggcctcgac
     3541 ggtatcgatg gtaccggtcc gcggatccgc ggccgcgact ctagatcagg cgcgccgtta
     3601 ccaagcagct atggaagctt atggacctca gagaggaagt aacgaggaga gggtgtggtg
     3661 gaatgccact agaaaccagg gaaaacaagg aggagagtat tacagggaag gaggtgaaga
     3721 acctcattac ccaaatactc ctgctcctca tagacgtacc tgggatgaga gacacaaggt
     3781 tcttaaattg tcctcattcg ctactccctc tgacatccaa cgctgggcta ctaactctag
     3841 attgtacggg agctctcttc actactcgct gcgtcgagag tgtacgagac tctccaggtt
     3901 tggtaagaaa tattttatat tgttataatg ttactatgat ccattaacac tctgcttata
     3961 gattgtaagg gtgattgcaa tgctttctgc ataaaacttt ggttttcttg ttaatcaata
     4021 aaccgacttg attcgagaac ctactcatat attattgtct cttttatact ttattaagta
     4081 aaaggatttg tatattagcc ttgctaaggg agacatctag tgatataagt gtgaactaca
     4141 cttatcttaa atgatgtaac tccttaggat aatcaatata caaaattcca tgacaattgg
     4201 cgataccgtc gaccgttcag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     4261 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     4321 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     4381 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     4441 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     4501 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     4561 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     4621 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     4681 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     4741 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     4801 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     4861 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     4921 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     4981 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     5041 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     5101 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     5161 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     5221 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     5281 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     5341 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     5401 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     5461 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     5521 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     5581 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     5641 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     5701 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     5761 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     5821 gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     5881 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     5941 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     6001 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     6061 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     6121 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     6181 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     6241 cctataaaaa taggcgtatc acgaggccct ttcgtcttca ctcgaggagc
//
