LOCUS       sequence-212300-        6290 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Deleted foamy virus vector plasmid containing a backbone with a 
            polylinker region for insertion of a transgene cassette. Ampicillin 
            resistant..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6290)
  AUTHORS   Trobridge G, Josephson N, Vassilopoulos G, Mac J, Russell DW
  TITLE     Improved foamy virus vectors with minimal viral sequences.
  JOURNAL   Mol Ther. 2002 Sep;6(3):321-8.
  PUBMED    12231167
REFERENCE   2  (bases 1 to 6290)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther.";
            date: "2002-09"; volume: "6(3)"; pages: "321-8"
COMMENT     Sequence Label: p-Delta-Phi
FEATURES             Location/Qualifiers
     source          1..6290
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        89..468
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        547..598
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     549..569
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(4296..4313)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4467..5055)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4547..4566)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5226..6017,6018..6086))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5849..5868
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6087..6191)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6259..6277
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctcgaggagc ttggcccatt gcatacgttg tatccatatc ataatatgta catttatatt
       61 ggctcatgtc caacattacc gccatgttga cattgattat tgactagtta ttaatagtaa
      121 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      181 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      241 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      301 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
      361 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
      421 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      481 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      541 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagcttc
      601 tagattgtac gggagctctc ttcactactc gctgcgtcga gagtgtacga gactctccag
      661 gtttggtaag aaatatttta tattgttata atgttactat gatccattaa cactctgctt
      721 atagattgta agggtgattg caatgctttc tgcataaaac tttggttttc ttgttaatca
      781 ataaaccgac ttgattcgag aacctactca tatattattg tctcttttat actttattaa
      841 gtaaaaggat ttgtatatta gccttgctaa gggagacatc tagtgatata agtgtgaact
      901 acacttatct taaatgatgt aactccttag gataatcaat atacaaaatt ccatgacaat
      961 tggcgcccaa cgtggggctc gaatataagt cgggtttatt tgtaaattat ccctagggac
     1021 ctccgagcat agcgggaggc atataaaagc caatagacaa tggctagcag gaagtaatgt
     1081 tgaagaatat gaacttgatg ttgaagctct ggttgtaatt ttaagagata gaaatatacc
     1141 aagaaatcct ttacatggag aagttatagg tcttcgcctt actgaaggat ggtggggaca
     1201 aattgagaga tttcagatgg tacgttgatc taaggctatg gatttggcca tgggacaaga
     1261 aatattagtt tatagtccca ttgtatctat gactaaaata caaaaaactc cactaccaga
     1321 aagaaaagct ttacccatta gatggataac atggatgact tatttagaag atccaagaat
     1381 ccaatttcat tatgataaaa ccttaccaga acttaagcat attccagatg tatatacatc
     1441 tagtcagtct cctgttaaac atccttctca atatgaagga gtgttttata ctgatggctc
     1501 ggccatcaaa agtcctgatc ctacaaaaag caataatgct ggcatgggaa tagtacatgc
     1561 cacatacaaa cctgaatatc aagttttgaa tcaatggtca ataccactag gtaatcatac
     1621 tgctcagatg gctgaaatag ctgcagttga atttgcctgt aaaaaagctt taaaaatacc
     1681 tggtcctgta ttagttataa ctgatagttt ctatgtagca gaaagtgcta ataaagaatt
     1741 accatactgg aaatctaatg ggtttgttaa taataagaaa aagcctctta aacatatctc
     1801 caaatggaag tctattgctg agtgtttatc tatgaaacca gacattacta ttcaacatga
     1861 aaaagggcat cagcctacaa ataccagtat tcatactgaa ggcaatgccc tagcagataa
     1921 gcttgccacc caaggaagtt atgtagttaa ttgtaatacc aaaaaaccaa acctggatgc
     1981 agagttggat caattattac agggtcatta tataaaagga tatcccaaac aatatacata
     2041 ttttttagaa gatggcaaag taaaagtttc cagacctgaa ggggttaaaa ttattccccc
     2101 tcagtcagac agacaaaaaa ttgtgcttca agcccacaat ttggctcaca ccggacgtga
     2161 agccactctt ttaaaaattg ccaaccttta ttggtggcca aatatgagaa aggatgtggt
     2221 taaacaacta ggacgctgtc aacagtgttt aatcacaaat gcttccaaca aagcctctgg
     2281 tcctattcta agaccagata ggcctcaaaa accttttgat aaattcttta ttgactatat
     2341 tggacctttg ccaccttcac agggatacct atatgtatta gtagttgttg atggaatgac
     2401 aggattcact tggttatacc ccactaaggc tccttctact agcgcaactg ttaaatctct
     2461 caatgtactc actagtattg caattccaaa ggtgattcac tctgatcaag gtgcagcatt
     2521 cacttcttca acctttgctg aatgggcaaa ggaaagaggt atacatttgg aattcagtac
     2581 tccttatcac ccccaaagta gtggtaaggt ggaaaggaaa aatagtgata taaaacgact
     2641 tttaactaaa ctgctagtag gaagacccac aaagtggtat gacctattgc ctgttgtaca
     2701 acttgcttta aacaacacct atagccctgt attaaaatat actccacatc aactcttatt
     2761 tggtatagat tcaaatactc catttgcaaa tcaagataca cttgacttga ccagagaaga
     2821 agaactttct cttttacagg aaattcgtac ttctttatac catccatcca cccctccaac
     2881 ctcctctcgt tcctggtctc ctgttgttgg ccaattggtc caggagaggg tggctaggcc
     2941 tgcttctttg agacctcgtt ggcataaacc gtctactgta cttaaggtgt tgaatccaag
     3001 gactgttgtt attttggacc atcttggcaa caacagaact gtaagtatag ataatttaaa
     3061 acctacttct catcagaatg gcaccaccaa tgacactgca acaatggatc atttggaaca
     3121 aaatgaataa agcgcatgag gcacttcaga atacaacaac tgtgactgaa cagcagaagg
     3181 aacaaattat actggacatt caaaatgaag aagtacaacc aactaggaga gataaattta
     3241 gatatctgct ttatacttgt tgtgctacta gctcaagagt attggcctgg atatttttag
     3301 tttgtatatt gttaatcatt gttttggttt catgctttgt gactatatcc agaatacaat
     3361 ggaataagga tattcaggta ttaggacctg taatagactg gaatgttact caaagagctg
     3421 tttatcaacc cttacagact agaaggattg cacgttccct tagaatgcag catcctgttc
     3481 caaaatatgt ggaggtaaat atgactagta ttccacaagg tgtatactat gaaccccatc
     3541 cggcctcgac ggtatcgatg gtaccggtcc gcggatccgc ggccgcgact ctagatcagg
     3601 cgcgccgtta ccaagcagct atggaagctt atggacctca gagaggaagt aacgaggaga
     3661 gggtgtggtg gaatgccact agaaaccagg gaaaacaagg aggagagtat tacagggaag
     3721 gaggtgaaga acctcattac ccaaatactc ctgctcctca tagacgtacc tgggatgaga
     3781 gacacaaggt tcttaaattg tcctcattcg ctactccctc tgacatccaa cgctgggcta
     3841 ctaactctag attgtacggg agctctcttc actactcgct gcgtcgagag tgtacgagac
     3901 tctccaggtt tggtaagaaa tattttatat tgttataatg ttactatgat ccattaacac
     3961 tctgcttata gattgtaagg gtgattgcaa tgctttctgc ataaaacttt ggttttcttg
     4021 ttaatcaata aaccgacttg attcgagaac ctactcatat attattgtct cttttatact
     4081 ttattaagta aaaggatttg tatattagcc ttgctaaggg agacatctag tgatataagt
     4141 gtgaactaca cttatcttaa atgatgtaac tccttaggat aatcaatata caaaattcca
     4201 tgacaattgg cgataccgtc gaccgttcag ctgcattaat gaatcggcca acgcgcgggg
     4261 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     4321 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     4381 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     4441 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     4501 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     4561 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     4621 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     4681 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     4741 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     4801 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     4861 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     4921 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     4981 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     5041 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     5101 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     5161 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     5221 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     5281 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     5341 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     5401 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     5461 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     5521 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     5581 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     5641 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     5701 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     5761 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     5821 agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa
     5881 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     5941 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     6001 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     6061 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     6121 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     6181 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     6241 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca
//
