LOCUS       sequence-212313-       10497 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor construct for introduction of NGN2 into AAVS1 safe harbor site
            and iPSC differentiation to cortical neuron, TALEN cut site outside 
            of homology arm.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10497)
  TITLE     A comprehensive guide for transcription-factor mediated 
            differentiation of human iPSC into neurons
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10497)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: H52-pUCM-hNGN2-inv
FEATURES             Location/Qualifiers
     source          1..10497
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     927..949
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1011..1030
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1062..1808
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(1899..1918)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1964..2019
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2018..2037)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     2329..2346
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2463..2484
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2499..2504,2505..2522,2523..2529)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2537..2553
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2542..2564
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2561..2577
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2561..2577
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    2626..2747
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2637..2656)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2691..2710
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3686..3710)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3755..3773
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3791..3809
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3827..3845
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3863..3881
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3899..3917
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3935..3953
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3971..3989
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    4022..4858
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     rep_origin      complement(4906..5494)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4986..5005)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5665..6456,6457..6525))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6288..6307
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     misc_feature    6670..7473
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     protein_bind    complement(7475..7508)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    7515..7540
                     /label=SA
                     /note="splice acceptor site"
     CDS             7564..7617
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7627..8226
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7627..7646)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8123..8143
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    8247..8471
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        8507..8718
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             8731..8999
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      9037..9046
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(9188..9206)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     9456..9475
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(9751..9784)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(9800..9817)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9806..10030
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        10036..10415
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact
       61 gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta
      121 gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct
      181 ccgggagggc cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt
      241 ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc
      301 ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg
      361 gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg
      421 agcagggggt gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc ctccccgagt
      481 tgctgagcac ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg cggggctcgc
      541 cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc
      601 cggggagggc tcgggggagg ggcgcggcgg cccccggagc gccggcggct gtcgaggcgc
      661 ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg gacttccttt
      721 gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg
      781 cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc
      841 gccgcgccgc cgtccccttc tccctctcca gcctcggggc tgtccgcggg gggacggctg
      901 ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg gcggctctag
      961 agcctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct
     1021 ggttattgtg ctgtctcatc attttggcaa agaatgtcga catgagccgg ctggacaaga
     1081 gcaaagtgat caacagcgcc ctggaactgc tgaacggcgt gggcatcgag ggcctgacca
     1141 cccggaagct ggcccagaaa ctgggcgtgg aacagcccac cctgtactgg cacgtgaaga
     1201 acaagcgggc cctgctggac gccctgccca tcgagatgct ggaccggcac cacacccaca
     1261 gctgccctct ggaaggcgag agctggcagg acttcctgcg gaacaacgcc aagagctaca
     1321 gatgcgccct gctgagccac cgggacggcg ccaaagtgca cctgggcacc agacccaccg
     1381 agaagcagta cgagacactg gaaaaccagc tggccttcct gtgccagcag ggcttcagcc
     1441 tggaaaacgc cctgtacgcc ctgagcgccg tgggccactt caccctgggc tgcgtgctgg
     1501 aagaacagga acaccaggtc gccaaagagg aaagagagac acccaccacc gacagcatgc
     1561 cccccctgct gaagcaggcc atcgagctgt tcgacagaca gggcgccgag cccgccttcc
     1621 tgttcggcct ggaactgatc atctgcggcc tcgagaagca gctgaagtgc gagagcggcg
     1681 gacccaccga cgccctggac gacttcgacc tggacatgct gcccgccgat gccctggatg
     1741 attttgatct ggatatgctc cctgctgacg ctctcgatga cttcgatctc gatatgctgc
     1801 caggctaaaa cgcgtgaatt cactcctcag gtgcaggctg cctatcagaa ggtggtggct
     1861 ggtgtggcca atgccctggc tcacaaatac cactgagatc tttttccctc tgccaaaaat
     1921 tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt
     1981 ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg
     2041 gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgcccat
     2101 atgctggctg ccatgaacaa aggttggcta taaagaggtc atcagtatat gaaacagccc
     2161 cctgctgtcc attccttatt ccatagaaaa gccttgactt gaggttagat tttttttata
     2221 ttttgttttg tgttattttt ttctttaaca tccctaaaat tttccttaca tgtgcggccg
     2281 tcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     2341 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     2401 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     2461 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     2521 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     2581 gattacgcca agctctagct agaggtcgac ggtatacaga catgataaga tacattgatg
     2641 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     2701 atgctattgc tttatttgta accattataa gctgcaataa acaagttggg gtgggcgatc
     2761 gattgcggcc gcttacaaga tacaatccct ggctatgggg aggtgaggtg catagcggtg
     2821 cttgtcggga ggtgggggct gccaatagtc catgtctgac ccggccgggc tggcgggcga
     2881 taaagtgcag ctgtaggggg aggtggaatt ggaggacacg gaggaggacg gcgcggggct
     2941 gttggtgcaa ctccacgtgg aggcgggcga ggggctgtct ccgctgctgc tcagggcggc
     3001 gctggcgcct cccgggctca gcaacactgc ctcggagaag agcgcccccg gcaggccccc
     3061 gccgccgccc ccgcagtgat ccgccaggcg cagggtctcg gtgagtgccc agatgtagtt
     3121 gtgggcgaag cgcagggtct cgatcttggt gagcttggcg tcctcgggga acgtggggag
     3181 cacctcgcgc agcgcgtcca gtgccgcgtt gaggttgtgc atgcggtttc gctcgcggtt
     3241 gttggccttc agtctacggg tcttcttgat gcgctgcacc gtctcggccg tcttggcgcc
     3301 tcgggagacg gcccgcgccc gggaagggcg ccgtttgcaa tcgtgtacca gacccagcag
     3361 ccgtgcgggc cggcagccct ccgcacccgc agccacgccg ccccgcgccc cctgcccggc
     3421 ctcagccccg cgctgccgac gcgccccgcc tgacgcgccc ggctcctcct cctcttcttc
     3481 gtcggcgctg gatgacagcg gggtcagggc cgccaaggcg ggggaggccg atccgagcag
     3541 cactaacacg tcctcttcct ccttcaactc caaggtctcg gatttgacga acatggtggc
     3601 ggcttaatta accttaagtt tacgagggta ggaagtggta cggaaagttg gtataagaca
     3661 aaagtgttgt ggaattgctc caggcgatct gacggttcac taaacgagct ctgcttttat
     3721 aggcgcccac cgtacacgcc taaagcttat acgttctcta tcactgatag ggagtaaact
     3781 ggatatacgt tctctatcac tgatagggag taaactgtag atacgttctc tatcactgat
     3841 agggagtaaa ctggtcatac gttctctatc actgataggg agtaaactcc ttatacgttc
     3901 tctatcactg atagggagta aagtctgcat acgttctcta tcactgatag ggagtaaact
     3961 cttcatacgt tctctatcac tgatagggag taaaccactg tggggtggag atatcagctt
     4021 tactagggac aggattggtg acagaaaagc cccatcctta ggcctcctcc ttcctagtct
     4081 cctgatattg ggtctaaccc ccacctcctg ttaggcagat tccttatctg gtgacacacc
     4141 cccatttcct ggagccatct ctctccttgc cagaacctct aaggtttgct tacgatggag
     4201 ccagagagga tcctgggagg gagagcttgg cagggggtgg gagggaaggg ggggatgcgt
     4261 gacctgcccg gttctcagtg gccaccctgc gctaccctct cccagaacct gagctgctct
     4321 gacgcggctg tctggtgcgt ttcactgatc ctggtgctgc agcttcctta cacttcccaa
     4381 gaggagaagc agtttggaaa aacaaaatca gaataagttg gtcctgagtt ctaactttgg
     4441 ctcttcacct ttctagtccc caatttatat tgttcctccg tgcgtcagtt ttacctgtga
     4501 gataaggcca gtagccagcc ccgtcctggc agggctgtgg tgaggagggg ggtgtccgtg
     4561 tggaaaactc cctttgtgag aatggtgcgt cctaggtgtt caccaggtcg tggccgcctc
     4621 tactcccttt ctctttctcc atccttcttt ccttaaagag tccccagtgc tatctgggac
     4681 atattcctcc gcccagagca gggtcccgct tccctaaggc cctgctctgg gcttctgggt
     4741 ttgagtcctt ggcaagccca ggagaggcgc tcaggcttcc ctgtccccct tcctcgtcca
     4801 ccatctcatg cccctggctc tcctgcccct tccctacagg ggttcctggc tctgctctaa
     4861 gggcgaattc gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     4921 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     4981 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     5041 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     5101 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     5161 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     5221 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     5281 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     5341 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     5401 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     5461 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     5521 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     5581 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     5641 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     5701 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     5761 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     5821 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     5881 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     5941 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     6001 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     6061 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     6121 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     6181 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     6241 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     6301 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     6361 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     6421 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     6481 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     6541 atattggcct aaggatgggg cttttctgtc accaatcctg gggccactag ggacactgtg
     6601 gggtggaggg gacagataaa agtacccggt accattattg aagcatttat cagggttgaa
     6661 ttcgcccttt gctttctctg accagcattc tctcccctgg gcctgtgccg ctttctgtct
     6721 gcagcttgtg gcctgggtca cctctacggc tggcccagat ccttccctgc cgcctccttc
     6781 aggttccgtc ttcctccact ccctcttccc cttgctctct gctgtgttgc tgcccaagga
     6841 tgctctttcc ggagcacttc cttctcggcg ctgcaccacg tgatgtcctc tgagcggatc
     6901 ctccccgtgt ctgggtcctc tccgggcatc tctcctccct cacccaaccc catgccgtct
     6961 tcactcgctg ggttcccttt tccttctcct tctggggcct gtgccatctc tcgtttctta
     7021 ggatggcctt ctccgacgga tgtctccctt gcgtcccgcc tccccttctt gtaggcctgc
     7081 atcatcaccg tttttctgga caaccccaaa gtaccccgtc tccctggctt tagccacctc
     7141 tccatcctct tgctttcttt gcctggacac cccgttctcc tgtggattcg ggtcacctct
     7201 cactcctttc atttgggcag ctcccctacc ccccttacct ctctagtctg tgctagctct
     7261 tccagccccc tgtcatggca tcttccaggg gtccgagagc tcagctagtc ttcttcctcc
     7321 aacccgggcc cctatgtcca cttcaggaca gcatgtttgc tgcctccagg gatcctgtgt
     7381 ccccgagctg ggaccacctt atattcccag ggccggttaa tgtggctctg gttctgggta
     7441 cttttatctg tcccctccac cccacagtgg ggcaataact tcgtatagca tacattatac
     7501 gaagttataa gcttctgacc tcttctcttc ctcccacagg gcctcgagag atctggcagc
     7561 ggagagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctagg
     7621 ctcgagatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
     7681 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     7741 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     7801 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
     7861 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     7921 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     7981 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     8041 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     8101 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     8161 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     8221 gcctgatcta gagggcccgt ttaaacctgt gccttctagt tgccagccat ctgttgtttg
     8281 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     8341 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     8401 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     8461 gggctctatg gatgcatgat ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca
     8521 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga
     8581 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag
     8641 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg
     8701 tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc acgcgcccgc
     8761 cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt
     8821 ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc gagaccgggc
     8881 ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac gctttgcctg
     8941 accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc
     9001 aagctgtgac cggcgcctac gctagcgcta ccggtcgcca ccatggtgag caagggcgag
     9061 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc
     9121 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
     9181 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg
     9241 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac
     9301 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     9361 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag
     9421 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg
     9481 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc
     9541 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac
     9601 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc
     9661 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac
     9721 tccaccggcg gcatggacga gtaaggtacc ataacttcgt atagcataca ttatacgaag
     9781 ttataaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     9841 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     9901 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     9961 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
    10021 ggctctatgg gtctcgacat tgattattga ctagttatta atagtaatca attacggggt
    10081 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
    10141 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
    10201 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
    10261 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
    10321 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
    10381 agtacatcta cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct
    10441 tcactctccc catctccccc ccctccccac ccccaatttt gtatttattt atttttt
//
