LOCUS       sequence-212339-        5921 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5921)
  TITLE     SGC Oxford April 2018 plasmids
REFERENCE   2  (bases 1 to 5921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: WNK3 (WNK3B-c001)
FEATURES             Location/Qualifiers
     source          1..5921
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..92
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     17..34
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             154..171
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             196..216
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     polyA_signal    1527..1661
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1564..1583)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1618..1637
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  1690..1855
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      2039..2493
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2126..2145)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2335..2356
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2519..2623
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2624..2692,2693..3484)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2842..2861)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3655..4243
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4144..4163
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4397..4414
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  4548..4772
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(4839..5372)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(5542..5547,5548..5564,5565..5570))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 atcatggaga taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc
       61 gtaacagttt tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca
      121 tcgggcgcgg atctcggtcc gaaaaccatg ggccaccatc atcatcatca ttcttctggt
      181 gtagatctgg gtaccgagaa cctgtacttc caatccatgg agaaaaatga aaaggaaatg
      241 gaagaagaag cagaaatgaa ggctgtagct acttctccta gtggcagatt cctgaaattt
      301 gacatagaac taggaagagg agcatttaaa acagtatata aaggactgga cactgaaaca
      361 tgggttgagg ttgcttggtg tgagctgcag gaccgaaagt taaccaaagc tgagcagcaa
      421 agattcaagg aagaagcaga gatgttgaag ggtctccagc accccaatat agttcgattt
      481 tatgattcct gggaatctat attaaaagga aagaaatgta ttgtattagt gactgaacta
      541 atgacatctg ggaccttaaa gacgtactta aaacgattta aagtcatgaa accaaaggtc
      601 ttaaggagct ggtgcaggca aattttaaag gggttgcagt tcttgcacac taggactcct
      661 cctattattc accgggatct gaagtgtgac aatattttca tcacgggacc cactggatct
      721 gtgaagattg gtgatctagg attagccacc ttaatgcgta cctcatttgc taagagtgtc
      781 attggaactc ctgagtttat ggctccagag atgtatgaag aacactatga tgaatccgta
      841 gatgtttatg cttttggaat gtgtatgctg gaaatggcca catcggagta tccttattct
      901 gagtgtcaga atgcagctca aatataccgg aaagtaacta gtggcataaa accagccagc
      961 ttcaataaag tcactgatcc tgaagtcaaa gaaatcattg aaggatgtat tcgtcaaaac
     1021 aaatctgaaa ggttgtctat cagggaccta ttaaaccatg cattttttgc tgaggataca
     1081 ggactgaggg tggagttagc agaagaagat gattgctcaa attcatccct tgctttaaga
     1141 ctctgggttg aagaccctaa aaaattgaaa ggcaaacaca aagacaatga agctattgaa
     1201 tttagtttca acttagaaac agatacacct gaggaagtag catatgaaat ggtcaagtct
     1261 gggttcttcc atgaaagtga ttccaaagct gttgctaaat ccattagaga ccgggtgacg
     1321 ccaataaaga agacaagaga gaagaagcct gcttgacagt aaaggtggat acggatccga
     1381 attcgagctc cgtcgacaag cttgtcgaga agtactagag gatcataatc agccatacca
     1441 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     1501 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     1561 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     1621 gtttgtccaa actcatcaat gtatcttatc atgtctggat ctgatcactg atatcgccta
     1681 ggagatccga accagataag tgaaatctag ttccaaacta ttttgtcatt tttaattttc
     1741 gtattagctt acgacgctac acccagttcc catctatttt gtcactcttc cctaaataat
     1801 ccttaaaaac tccatttcca cccctcccag ttcccaacta ttttgtccgc ccacagcggg
     1861 gcatttttct tcctgttatg tttttaatca aacatcctgc caactccatg tgacaaaccg
     1921 tcatcttcgg ctactttttc tctgtcacag aatgaaaatt tttctgtcat ctcttcgtta
     1981 ttaatgtttg taattgactg aatatcaacg cttatttgca gcctgaatgg cgaatgggac
     2041 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     2101 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     2161 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     2221 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     2281 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt aatagtggac
     2341 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     2401 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     2461 cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg
     2521 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     2581 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     2641 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     2701 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     2761 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     2821 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     2881 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     2941 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     3001 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     3061 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     3121 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     3181 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     3241 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     3301 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     3361 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     3421 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     3481 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     3541 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     3601 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     3661 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     3721 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     3781 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     3841 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     3901 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     3961 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     4021 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     4081 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     4141 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     4201 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     4261 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     4321 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     4381 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta
     4441 ttttctcctt acgcatctgt gcggtatttc acaccgcata gaccagccgc gtaacctggc
     4501 aaaatcggtt acggttgagt aataaatgga tgccctgcgt aagcgggtgt gggcggacaa
     4561 taaagtctta aactgaacaa aatagatcta aactatgaca ataaagtctt aaactagaca
     4621 gaatagttgt aaactgaaat cagtccagtt atgctgtgaa aaagcatact ggacttttgt
     4681 tatggctaaa gcaaactctt cattttctga agtgcaaatt gcccgtcgta ttaaagaggg
     4741 gcgtggccaa gggcatggta aagactatat tcgcggcgtt gtgacaattt accgaacaac
     4801 tccgcggccg ggaagccgat ctcggcttga acgaattgtt aggtggcggt acttgggtcg
     4861 atatcaaagt gcatcacttc ttcccgtatg cccaactttg tatagagagc cactgcggga
     4921 tcgtcaccgt aatctgcttg cacgtagatc acataagcac caagcgcgtt ggcctcatgc
     4981 ttgagcagat tgatgagcgc ggtggcaatg ccctgcctcc ggtgctcgcc ggagactgcg
     5041 agatcataga tatagatctc actacgcggc tgctcaaact tgggcagaac gtaagccgcg
     5101 agagcgccaa caaccgcttc ttggtcgaag gcagcaagcg cgatgaatgt cttactacgg
     5161 agcaagttcc cgaggtaatc ggagtccggc tgatgttggg agtaggtggc tacgtctccg
     5221 aactcacgac cgaaaagatc aagagcagcc cgcatggatt tgacttggtc agggccgagc
     5281 ctacatgtgc gaatgatgcc catacttgag ccacctaact ttgttttagg gcgactgccc
     5341 tgctgcgtaa catcgttgct gctccataac atcaaacatc gacccacggc gtaacgcgct
     5401 tgctgcttgg atgcccgagg catagactgt acaaaaaaac agtcataaca agccatgaaa
     5461 accgccactg cgccgttacc accgctgcgt tcggtcaagg ttctggacca gttgcgtgag
     5521 cgcatacgct acttgcatta cagtttacga accgaacagg cttatgtcaa ctgggttcgt
     5581 gccttcatcc gtttccacgg tgtgcgtcac ccggcaacct tgggcagcag cgaagtcgag
     5641 gcatttctgt cctggctggc gaacgagcgc aaggtttcgg tctccacgca tcgtcaggca
     5701 ttggcggcct tgctgttctt ctacggcaag gtgctgtgca cggatctgcc ctggcttcag
     5761 gagatcggaa gacctcggcc gtcgcggcgc ttgccggtgg tgctgacccc ggatgaagtg
     5821 gttcgcatcc tcggttttct ggaaggcgag catcgtttgt tcgcccagga ctctagctat
     5881 agttctagtg gttggctacg tatactccgg aatattaata g
//
