LOCUS       sequence-212346-       11964 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of dAtg1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11964)
  AUTHORS   Kim M, Park HL, Park HW, Ro SH, Nam SG, Reed JM, Guan JL, Lee JH
  TITLE     Drosophila Fip200 is an essential regulator of autophagy that 
            attenuates both growth and aging.
  JOURNAL   Autophagy. 2013 Aug;9(8):1201-13. doi: 10.4161/auto.24811. Epub 2013
            May 14.
  PUBMED    23819996
REFERENCE   2  (bases 1 to 11964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.4161/auto.24811"; journalName: "Autophagy"; date: "2013-08"; 
            volume: "9"; issue: "8"; pages: "1201-13"
COMMENT     Sequence Label: pUAST-Myc-dATG1
FEATURES             Location/Qualifiers
     source          1..11964
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(634..653)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     753..775
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(813..831)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        899..1003
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1004..1072,1073..1864)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1222..1241)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2035..2623
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2524..2543
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    join(2858..2888,2889..3443)
                     /label=P element 5' end
     misc_feature    join(4299..4329,4330..4531)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    4593..4687
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        4706..4944
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     4891..4912
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     regulatory      4963..4972
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4972..5001
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(6150..6161)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     intron          7671..7736
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7866..7886
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8158..8292
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8195..8214)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8249..8268
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8319..8337)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(8789..8860,9262..9535,9610..10264,10326..10641,
                     10845..10976,11047..11661)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
ORIGIN
        1 cactcagaat actattcctt tcactcgcac ttattgcaag catacgttaa gtggatgtct
       61 cttgccgacg ggaccacctt atgttatttc atcatggtct ggccattctc atcgtgagct
      121 tccgggtgct cgcatatctg gctctaagac ttcgggcccg acgcaaggag tagccgacat
      181 atatccgaaa taactgcttg tttttttttt ttaccattat taccatcgtg tttactgttt
      241 attgccccct caaaaagcta atgtaattat atttgtgcca ataaaaacaa gatatgacct
      301 atagaataca agtatttccc cttcgaacat ccccacaagt agactttgga tttgtcttct
      361 aaccaaaaga cttacacacc tgcatacctt acatcaaaaa ctcgtttatc gctacataaa
      421 acaccgggat atatttttta tatacatact tttcaaatcg cgcgccctct tcataattca
      481 cctccaccac accacgtttc gtagttgctc tttcgctgtc tcccacccgc tctccgcaac
      541 acattcacct tttgttcgac gaccttggag cgactgtcgt tagttccgcg cgattcggtg
      601 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
      661 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
      721 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
      781 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
      841 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
      901 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
      961 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     1021 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     1081 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     1141 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     1201 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     1261 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     1321 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     1381 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     1441 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     1501 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     1561 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     1621 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     1681 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     1741 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     1801 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     1861 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     1921 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     1981 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     2041 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     2101 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     2161 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     2221 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     2281 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     2341 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     2401 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     2461 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     2521 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     2581 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcct tcttcttgaa
     2641 ctcgggctcg gtgccagtat acctcaaatg gttgtcgtac ctctcatggt tccgttacgc
     2701 caacgagggt ctgctgatta accaatgggc ggacgtggag ccgggcgaaa ttagctgcac
     2761 atcgtcgaac accacgtgcc ccagttcggg caaggtcatc ctggagacgc ttaacttctc
     2821 cgccgccgat ctgccgctgg actacgtggg tctggcccat gatgaaataa cataaggtgg
     2881 tcccgtcgat agccgaagct taccgaagta tacacttaaa ttcagtgcac gtttgcttgt
     2941 tgagaggaaa ggttgtgtgc ggacgaattt ttttttgaaa acattaaccc ttacgtggaa
     3001 taaaaaaaaa tgaaatattg caaattttgc tgcaaagctg tgactggagt aaaattaatt
     3061 cacgtgccga agtgtgctat taagagaaaa ttgtgggagc agagccttgg gtgcagcctt
     3121 ggtgaaaact cccaaatttg tgatacccac tttaatgatt cgcagtggaa ggctgcacct
     3181 gcaaaaggtc agacatttaa aaggaggcga ctcaacgcag atgccgtacc tagtaaagtg
     3241 atagagcctg aaccagaaaa gataaaagaa ggctatacca gtgggagtac acaaacagag
     3301 taagtttgaa tagtaaaaaa aatcatttat gtaaacaata acgtgactgt gcgttaggtc
     3361 ctgttcattg tttaatgaaa ataagagctt gagggaaaaa attcgtactt tggagtacga
     3421 aatgcgtcgt ttagagcagc agccgaatta attctagttc cagtgaaatc caagcatttt
     3481 ctaaattaaa tgtattctta ttattatagt tgttattttt gatatatata aacaacacta
     3541 ttatgcccac catttttttg agatgcatct acacaaggaa caaacactgg atgtcacttt
     3601 cagttcaaat tgtaacgcta atcactccga acaggtcaca aaaaattacc ttaaaaagtc
     3661 ataatattaa attagaataa atatagctgt gagggaaata tatacaaata tattggagca
     3721 aataaattgt acatacaaat atttattact aatttctatt gagacgaaat gaaccactcg
     3781 gaaccatttg agcgaaccga atcgcgcgga actaacgaca gtcgctccaa ggtcgtcgaa
     3841 caaaaggtga atgtgttgcg gagagcgggt gggagacagc gaaagagcaa ctacgaaacg
     3901 tggtgtggtg gaggtgaatt atgaagaggg cgcgcgattt gaaaagtatg tatataaaaa
     3961 atatatcccg gtgttttatg tagcgataaa cgagtttttg atgtaaggta tgcaggtgtg
     4021 taagtctttt ggttagaaga caaatccaaa gtctacttgt ggggatgttc gaaggggaaa
     4081 tacttgtatt ctataggtca tatcttgttt ttattggcac aaatataatt acattagctt
     4141 tttgaggggg caataaacag taaacacgat ggtaataatg gtaaaaaaaa aaaacaagca
     4201 gttatttcgg atatatgtcg gctactcctt gcgtcgggcc cgaagtctta gagccagata
     4261 tgcgagcacc cggaagctca cgatgagaat ggccagacca tgatgaaata acataaggtg
     4321 gtcccgtcgg caagagacat ccacttaacg tatgcttgca ataagtgcga gtgaaaggaa
     4381 tagtattctg agtgtcgtat tgagtctgag tgagacagcg atatgattgt tgattaaccc
     4441 ttagcatgtc cgtggggttt gaattaactc ataatattaa ttagacgaaa ttatttttaa
     4501 agttttattt ttaataattt gcgagtacgc aaagcttggc tgcatccaac gcgttgggag
     4561 ctctccggat ccaagcttgc atgcctgcag gtcggagtac tgtcctccga gcggagtact
     4621 gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg gagtactgtc
     4681 ctccgagcgg agactctagc gagcgccgga gtataaatag aggcgcttcg tctacggagc
     4741 gacaattcaa ttcaaacaag caaagtgaac acgtcgctaa gcgaaagcta agcaaataaa
     4801 caagcgcagc tgaacaagct aaacaatctg cagtaaagtg caagttaaag tgaatcaatt
     4861 aaaagtaacc agcaaccaag taaatcaact gcaactactg aaatctgcca agaagtaatt
     4921 attgaataca agaagagaac tctgaatagg gaattgggaa ttgccaccat ggaacagaaa
     4981 ctcatctctg aagaggatct ggaattcatg aatattgtcg gggaatatga atacagctcc
     5041 aaggatatgc tgggccatgg cgcctttgcc gtcgtctaca aaggacgtca tcgcaagaaa
     5101 cacatgccgg tggccatcaa gtgcatcacg aagaagggac aactgaagac gcagaatctg
     5161 ctcggcaagg agatcaagat cctgaaggag ctcaccgagc tgcaccatga gaatgtggtg
     5221 gctctgctgg actgcaagga gtcccaggat tgcgtcagcc tggtcatgga gtattgcaat
     5281 ggcggcgact tggcggatta tctgagtgtc aaggggacgc tcagcgagga taccgttaga
     5341 ctcttcctcg tgcaactagc tggtgctatg aaagcacttt ataccaaagg aattgtgcat
     5401 cgtgatctca agccacaaaa cattttgcta tcgcacaatt atggcaaaac attgccagct
     5461 ccatcgaaaa taaccctgaa aattgcggat tttgggtttg cgcgattcct gaacgagggc
     5521 gccatggcag ccactctgtg cggctctccc atgtatatgg cgcccgaggt gatcatgtcg
     5581 ctgcagtacg actccaaggc ggatctctgg tcgctgggaa cgattgtcta ccagtgtttg
     5641 actggcaagg cgccattcta tgcacaaacg cccaacgaac tgaagtccta ctacgagcag
     5701 aatgctaacc tggcaccaaa aattccaagt ggcgtgtcac cggatctgag ggatctgctg
     5761 ctgtgcctgc tgcgccgcaa ctccaaggat cgcatctcgt acgagagctt ctttgttcac
     5821 cgcttccttc agggcaagaa ggcggccgtg tcgccaggtg agcctagtca agcagccaac
     5881 ctgcgacgac gagtctctaa gagtggtgtt cttccagttg acatgccacc gcttggcgga
     5941 acaccacctg ccaaggccaa gagccccttg cagcagcagc tggagcagga actgaagctg
     6001 gtcaagctgg ccgagcaaca gcagaaggag cgcgaggagc aggaggccca ggaggacgag
     6061 aacacggtgt ccgtggtggc caatccggcc atttgtgcca ccatcaccaa cgtaggtgtc
     6121 ctgtgcgaca gtgagaacaa cagcggttct tgcagctccc acgaggactc cgatgacttt
     6181 gtgctggtgc caaagaacct tccggaggac cagcgacagg gtctggccca agtccaagcc
     6241 cagcccgctt ccggtggcca acgtccacag cagcaacaaa atcaatccag tccgccacgt
     6301 cccagcagtc tgcccatctc tgaacccaaa ccagtaccgg ctcccgctcg gcggcaggtg
     6361 gccaggcccg ggccgttaac ggtagccaca ctgggtggcc agcagatacc ccgatcgcag
     6421 ccaattagcg taaagcaacc gcgaccggat cagcgcaaga gcagtgtgag cagtgacatt
     6481 aactcgatct caccgccggc ggtgcagttt gccattggaa cgccgcctac acgtatgcgt
     6541 tctgcctctg gtggatcgct gtcggagaca ccgccgccgc atgcaccgag cacctggcag
     6601 gtgtcgccag gacactcgca atcgccgttg cgtcgaagcg gcaatagctc gccagttctg
     6661 ccatccgctg cgcttaccaa attgcccact ttgggcagtc caacaatgct ggtggcgccg
     6721 ggatcattgg gctcgattgg ttcggcgggc agtggatcgg agaacaacaa tcagcatcac
     6781 atgctgggac cgcgagcttt tacgctgccc gaactggggg ccactggtgg cctacatagt
     6841 ctgctggaca caggagccgg cggcggtggg gaaccacatg cattccaggc cccagaactg
     6901 tccgaagaga cgctgatgga tcgcgagcac aacgagacat tgtccaagct gaactttgtg
     6961 ctggcgctca ccgattgtat ccaggaagtt gccgattcgc gatgcgcacc gctctccacg
     7021 ttcatggtgg ccggcagtca gtcggcggcg caagcggcgt cagcggatgc acagcagata
     7081 ccgccgcacg cacctgagca ctgcaaacgg gccgagcgcc tggtgctgct cgtccgggga
     7141 ctgcagctcc tatcgtccgg gatgaatctg gcttcccagc agctaagcaa tggccagcta
     7201 aagccgtcgt ccaatgtgaa gaatgctttg ctcaccatga acgccaaata ccggagcatg
     7261 ctgttcgaat cgaaacgcct caatggcagc ggactattgc agaaggcgaa tgcatttaac
     7321 ataacggcgg acaagattct ctatgactac gcactggata tgtgccaggc cgctgctctg
     7381 gatgagctac tgaagaacac gaagaattgc ttcgaacgtt acaacacggc acacattctg
     7441 cttcattcgt tggtgcaaaa gtgcaatcat ccgcaggaca aaatgatgct gaacaaatat
     7501 cgcgatgccg ttgagaagcg tttgagcata ttgcagcagc atggctatat atacatgacc
     7561 gatgagaatg cttagctcga gggtacctct agaggatctt tgtgaaggaa ccttacttct
     7621 gtggtgtgac ataattggac aaactaccta cagagattta aagctctaag gtaaatataa
     7681 aatttttaag tgtataatgt gttaaactac tgattctaat tgtttgtgta ttttagattc
     7741 caacctatgg aactgatgaa tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt
     7801 tttgctcaga agaaatgcca tctagtgatg atgaggctac tgctgactct caacattcta
     7861 ctcctccaaa aaagaagaga aaggtagaag accccaagga ctttccttca gaattgctaa
     7921 gttttttgag tcatgctgtg tttagtaata gaactcttgc ttgctttgct atttacacca
     7981 caaaggaaaa agctgcactg ctatacaaga aaattatgga aaaatatttg atgtatagtg
     8041 ccttgactag agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     8101 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     8161 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     8221 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     8281 catgtctgga tccactagaa ggcctaattc tagtatgtat gtaagttaat aaaacccatt
     8341 tttgcggaaa gtagataaaa aaaacatttt ttttttttac tgcactggat atcattgaac
     8401 ttatctgatc agttttaaat ttacttcgat ccaagggtat ttgatgtacc aggttctttc
     8461 gattacctct cactcaaaat gacattccac tcaaagtcag cgctgtttgc ctccttctct
     8521 gtccacagaa atatcgccgt ctctttcgcc gctgcgtccg ctatctcttt cgccaccgtt
     8581 tgtagcgtta cgtagcgtca atgtccgcct tcagttgcat tttgtcagcg gtttcgtgac
     8641 gaagctccaa gcggtttacg ccatcaatta aacacaaagt gctgtgccaa aactcctctc
     8701 gcttcttatt tttgtttgtt ttttgagtga ttggggtggt gattggtttt gggtgggtaa
     8761 gcaggggaaa gtgtgaaaaa tcccggcaat gggccaagag gatcaggagc tattaattcg
     8821 cggaggcagc aaacacccat ctgccgagca tctgaacaat gtgagtagta catgtgcata
     8881 catcttaagt tcacttgatc tataggaact gcgattgcaa catcaaattg tctgcggcgt
     8941 gagaactgcg acccacaaaa atcccaaacc gcaattgcac aaacaaatag tgacacgaaa
     9001 cagattattc tggtagctgt tctcgctata taagacaatt tttgagatca tatcatgatc
     9061 aagacatcta aaggcattca ttttcgacta tattcttttt tacaaaaaat ataacaacca
     9121 gatattttaa gctgatccta gatgcacaaa aaataaataa aagtataaac ctacttcgta
     9181 ggatacttcg gggtactttt tgttcggggt tagatgagca taacgcttgt agttgatatt
     9241 tgagatcccc tatcattgca gggtgacagc ggagcggctt cgcagagctg cattaaccag
     9301 ggcttcgggc aggccaaaaa ctacggcacg ctccggccac ccagtccgcc ggaggactcc
     9361 ggttcaggga gcggccaact agccgagaac ctcacctatg cctggcacaa tatggacatc
     9421 tttggggcgg tcaatcagcc gggctccgga tggcggcagc tggtcaaccg gacacgcgga
     9481 ctattctgca acgagcgaca cataccggcg cccaggaaac atttgctcaa gaacggtgag
     9541 tttctattcg cagtcggctg atctgtgtga aatcttaata aagggtccaa ttaccaattt
     9601 gaaactcagt ttgcggcgtg gcctatccgg gcgaactttt ggccgtgatg ggcagttccg
     9661 gtgccggaaa gacgaccctg ctgaatgccc ttgcctttcg atcgccgcag ggcatccaag
     9721 tatcgccatc cgggatgcga ctgctcaatg gccaacctgt ggacgccaag gagatgcagg
     9781 ccaggtgcgc ctatgtccag caggatgacc tctttatcgg ctccctaacg gccagggaac
     9841 acctgatttt ccaagccatg gtgcggatgc cacgacatct gacctatcgg cagcgagtgg
     9901 cccgcgtgga tcaggtgatc caggagcttt cgctcagcaa atgtcagcac acgatcatcg
     9961 gtgtgcccgg cagggtgaaa ggtctgtccg gcggagaaag gaagcgtctg gcattcgcct
    10021 ccgaggctct aaccgatccg ccgcttctga tctgcgatga gcccacctcc ggactggact
    10081 cctttaccgc ccacagcgtc gtccaggtgc tgaagaagct gtcgcagaag ggcaagaccg
    10141 tcatcctgac cattcatcag ccgtcttccg agctgtttga gctctttgac aagatccttc
    10201 tgatggccga gggcagggta gctttcttgg gcactcccag cgaagccgtc gacttctttt
    10261 cctagtgagt tcgatgtgtt tattaagggt atctagtatt acataacatc tcaactccta
    10321 tccagcgtgg gtgcccagtg tcctaccaac tacaatccgg cggactttta cgtacaggtg
    10381 ttggccgttg tgcccggacg ggagatcgag tcccgtgatc ggatcgccaa gatatgcgac
    10441 aattttgcca ttagcaaagt agcccgggat atggagcagt tgttggccac caaaaatctg
    10501 gagaagccac tggagcagcc ggagaatggg tacacctaca aggccacctg gttcatgcag
    10561 ttccgggcgg tcctgtggcg atcctggctg tcggtgctca aggaaccact cctcgtaaaa
    10621 gtgcgactta ttcagacaac ggtgagtggt tccagtggaa acaaatgata taacgcttac
    10681 aattcttgga aacaaattcg ctagatttta gatagaattg cctgattcca cacccttctt
    10741 agtttttttc aatgagatgt atagtttata gttttgcaga agataaataa atttcattta
    10801 actcgcgaat attaatgaga tgcgagtaac attttaattt gcagatggtt gccatcttga
    10861 ttggcctcat ctttttgggc caacaactca cgcaagtggg tgtgatgaat atcaacggag
    10921 ccatcttcct cttcctgacc aacatgacct ttcaaaacgt ctttgccacg ataaatgtaa
    10981 gtcatgttta gaatacattt gcatttcaat aatttactaa ctttctaatg aatcgattcg
    11041 atttaggtgt tcacctcaga gctgccagtt tttatgaggg aggcccgaag tcgactttat
    11101 cgctgtgaca catactttct gggcaaaacg attgccgaat tgccgctttt tctcacagtg
    11161 ccactggtct tcacggcgat tgcctatccg atgatcggac tgcgggccgg agtgctgcac
    11221 ttcttcaact gcctggcgct ggtcactctg gtggccaatg tgtcaacgtc cttcggatat
    11281 ctaatatcct gcgccagctc ctcgacctcg atggcgctgt ctgtgggtcc gccggttatc
    11341 ataccattcc tgctctttgg cggcttcttc ttgaactcgg gctcggtgcc agtatacctc
    11401 aaatggttgt cgtacctctc atggttccgt tacgccaacg agggtctgct gattaaccaa
    11461 tgggcggacg tggagccggg cgaaattagc tgcacatcgt cgaacaccac gtgccccagt
    11521 tcgggcaagg tcatcctgga gacgcttaac ttctccgccg ccgatctgcc gctggactac
    11581 gtgggtctgg ccattctcat cgtgagcttc cgggtgctcg catatctggc tctaagactt
    11641 cgggcccgac gcaaggagta gccgacatat atccgaaata actgcttgtt tttttttttt
    11701 accattatta ccatcgtgtt tactgtttat tgccccctca aaaagctaat gtaattatat
    11761 ttgtgccaat aaaaacaaga tatgacctat agaatacaag tatttcccct tcgaacatcc
    11821 ccacaagtag actttggatt tgtcttctaa ccaaaagact tacacacctg cataccttac
    11881 atcaaaaact cgtttatcgc tacataaaac accgggatat attttttata tacatacttt
    11941 tcaaatcgcg cgccctcttc ataa
//
