LOCUS       sequence-212347-       12255 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pSIN - EF-1alpha - PIF3 - MTAD - IRES - PhyB - GBD, dual vector of 
            PIF3-MTAD under EF-1 alpha promoter and PhyB-DBD under IRES 
            promoter; See growth conditions below..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12255)
  AUTHORS   Kyriakakis P, Catanho M, Hoffner N, Thavarajah W, Hu VJ, Chao SS, 
            Hsu A, Pham V, Naghavian L, Dozier LE, Patrick GN, Coleman TP
  TITLE     Biosynthesis of Orthogonal Molecules Using Ferredoxin and 
            Ferredoxin-NADP(+) Reductase Systems Enables Genetically Encoded 
            PhyB Optogenetics.
  JOURNAL   ACS Synth Biol. 2018 Feb 16;7(2):706-717. doi: 
            10.1021/acssynbio.7b00413. Epub 2018 Jan 24.
  PUBMED    29301067
REFERENCE   2  (bases 1 to 12255)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.7b00413"; journalName: "ACS Synth Biol"; date: 
            "2018-02-16- 16"; volume: "7"; issue: "2"; pages: "706-717"
COMMENT     Sequence Label: pPKm-230
FEATURES             Location/Qualifiers
     source          1..12255
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    493..726
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             911..955
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1242..1359
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1488..2666
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1719..2657
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2614..2634
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4509..4520
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             4536..4556
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4569..4595
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    join(4608..5183,5184..5186,5187..5195)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4776..4793)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5003..5022
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7083..7103
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7113..7553
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     primer_bind     complement(7621..7641)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7626..7955
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7806..7941
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7965..8564
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7965..7984)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8461..8481
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             8857..9090
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9241..9258)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9247..9471
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     regulatory      9514..9523
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        9567..9671
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9672..9740,9741..10532)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9890..9909)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10703..11291
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11192..11211
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             11450..12083
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    12130..12255
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
       61 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
      121 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
      181 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
      241 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
      301 gaagagcaaa acaaaagtaa gaccaccgca cagcaaccgg ccgctgatct tcagacctgg
      361 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
      421 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
      481 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
      541 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
      601 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
      661 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
      721 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
      781 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
      841 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
      901 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
      961 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     1021 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     1081 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
     1141 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
     1201 atctcgacgg tatcgccaca aatggcagta ttcatccaca attttaaaag aaaggggggg
     1261 attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact
     1321 aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc
     1381 agagatccac tttggatcga taagctttgc aaagatggat aaagttttaa acagagagga
     1441 atctttgcag ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc
     1501 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
     1561 attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
     1621 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
     1681 acgttctttt tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc
     1741 gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc
     1801 tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc
     1861 cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg
     1921 gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc
     1981 tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg
     2041 taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg
     2101 gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga
     2161 gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc
     2221 cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg
     2281 aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg
     2341 gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg
     2401 cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct
     2461 tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca
     2521 ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat
     2581 ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt
     2641 tttttcttcc atttcaggtg tcgtgaggaa ttgatccttc gaactagaat tcgaagcttg
     2701 agctcgagat ctgagtccgg tagcgctagt tcgaaggatc cgccgccacc atgcctctgt
     2761 ttgagctttt caggctcacc aaagctaagc ttgaatctgc tcaagacagg aacccttctc
     2821 cacctgtaga tgaagttgtg gagctggtgt gggaaaatgg tcagatatca actcaaagtc
     2881 agtcaagtag atcgaggaac attcctccac cacaagcaaa ctcttctaga gctagagaga
     2941 ttggaaatgg ctcaaagacg actatggtgg acgagatccc tatgtcagtg ccatcactaa
     3001 tgacgggttt gagtcaagac gatgactttg ttccatggtt gaatcatcat ccctcccttg
     3061 atggatattg ctctgatttc ttgcgtgatg tgtcgtctcc tgttactgtc aacgagcaag
     3121 agagtgatat ggcggtaaac caaactgctt tcccgttgtt tcagagaaga aaggatggca
     3181 atgaatcagc tcctgctgct tcttcgtcgc agtataacgg tttccaatcg cattctctgt
     3241 atggaagtga tagagctaga gatcttccta gccaacaaac caatccggat cggtttactc
     3301 agacgcagga accactaatt actagtaaca agcctagttt ggtcaacttt tcacatttct
     3361 tacgccctgc aacttttgcg aagactacta ataataacct tcatgacact aaagaaaaga
     3421 gtcctcaaag cccgccaaat gtgtttcaga ccagagttct tggagctaaa gactctgaag
     3481 ataaggttct taacgagtct gttgcttctg ctacgcctaa agataaccaa aaggcttgcc
     3541 taatatcaga ggactcatgt agaaaagacc aagagagtga aaaagcagtt gtatgttctt
     3601 ctgttggctc gggtaatagt ctcgatggcc catccgaaag tccttcactt tctttaaaga
     3661 gaaagcattc gaatattcaa gacattgact gtcatagtga agatgtggaa gaagaatcag
     3721 gagatggaag aaaggaagca ggtccatctc gaacgggttt gggttcaaag agaagccgct
     3781 ctgcagaagt gcataatctg tctgaaagga gacggcgtga taggatcaac gagaagatgc
     3841 gtgccctgca agaactcatt ccaaactgta acaaggtgga caaagcttcg atgctagatg
     3901 aagccatcga gtatctcaag tcactccaac ttcaagtgca gatcatgtca atggcgtctg
     3961 gttactatct gccaccggcg gttatgttcc caccgggtat ggggcattac ccggcagcag
     4021 ctgctgcaat ggcaatgggt atgggaatgc cttatgcaat gggcttgcct gatttgagcc
     4081 gtggtggttc atcggttaac cacggaccac agttccaagt ctcggggatg caacaacaac
     4141 cagtggcgat gggtattcca cgtgtctctg gtggtggtat ctttgccggt tcttcgacga
     4201 ttggcaatgg ctcgactaga gatttatctg gttctaaaga tcaaacaacg acgaataaca
     4261 acagtaactt gaaaccaata aagagaaaac aggggtcttc tgatcagttt tgtggatcgt
     4321 ggcgcgccat gggatctcct aggtttgata ctgacctgtt cgataccgac ttgtttgata
     4381 ctgacctttt tgatactgac ctgttcgata ccgacttgtt tgatactgac ctttttgata
     4441 ctgacctgtt cgataccgac ttgtttgata ctgacctttt tgatactgac ctgttcgata
     4501 ccgacttgat cgagggaagg ggtggcgaat tcatgcctaa gaaaaagaga aaggtgggcg
     4561 gaatggctta cccatacgat gttccagatt acgcttgata acatatgccc ctctccctcc
     4621 ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta
     4681 tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc
     4741 tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct
     4801 gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt
     4861 agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa
     4921 gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg
     4981 gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga
     5041 tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtaca catgctttac
     5101 atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt
     5161 cctttgaaaa acacgatgat aatatggcca caaccactag ttcgcgaatg gtttccggag
     5221 tcgggggtag tggcggtggc cgtggcggtg gccgtggcgg agaagaagaa ccgtcgtcaa
     5281 gtcacactcc taataaccga agaggaggag aacaagctca atcgtcggga acgaaatctc
     5341 tcagaccaag aagcaacact gaatcaatga gcaaagcaat tcaacagtac accgtcgacg
     5401 caagactcca cgccgttttc gaacaatccg gcgaatcagg gaaatcattc gactactcac
     5461 aatcactcaa aacgacgacg tacggttcct ctgtacctga gcaacagatc acagcttatc
     5521 tctctcgaat ccagcgaggt ggttacattc agcctttcgg atgtatgatc gccgtcgatg
     5581 aatccagttt ccggatcatc ggttacagtg aaaacgccag agaaatgtta gggattatgc
     5641 ctcaatctgt tcctactctt gagaaacctg agattctagc tatgggaact gatgtgagat
     5701 ctttgttcac ttcttcgagc tcgattctac tcgagcgtgc tttcgttgct cgagagatta
     5761 ccttgttaaa tccggtttgg atacattcca agaatactgg taaaccgttt tacgccattc
     5821 ttcataggat tgatgttggt gttgttattg atttagagcc agctagaact gaagatcctg
     5881 cgctttctat tgctggtgct gttcaatcgc agaaactcgc ggttcgtgcg atttctcagt
     5941 tacaggctct tcctggtgga gatattaagc ttttgtgtga cactgtcgtg gaaagtgtga
     6001 gggacttgac tggttatgat cgtgttatgg tttataagtt tcatgaagat gagcatggag
     6061 aagttgtagc tgagagtaaa cgagacgatt tagagcctta tattggactg cattatcctg
     6121 ctactgatat tcctcaagcg tcaaggttct tgtttaagca gaaccgtgtc cgaatgatag
     6181 tagattgcaa tgccacacct gttcttgtgg tccaggacga taggctaact cagtctatgt
     6241 gcttggttgg ttctactctt agggctcctc atggttgtca ctctcagtat atggctaaca
     6301 tgggatctat tgcgtcttta gcaatggcgg ttataatcaa tggaaatgaa gatgatggga
     6361 gcaatgtagc tagtggaaga agctcgatga ggctttgggg tttggttgtt tgccatcaca
     6421 cttcttctcg ctgcataccg tttccgctaa ggtatgcttg tgagtttttg atgcaggctt
     6481 tcggtttaca gttaaacatg gaattgcagt tagctttgca aatgtcagag aaacgcgttt
     6541 tgagaacgca gacactgtta tgtgatatgc ttctgcgtga ctcgcctgct ggaattgtta
     6601 cacagagtcc cagtatcatg gacttagtga aatgtgacgg tgcagcattt ctttaccacg
     6661 ggaagtatta cccgttgggt gttgctccta gtgaagttca gataaaagat gttgtggagt
     6721 ggttgcttgc gaatcatgcg gattcaaccg gattaagcac tgatagttta ggcgatgcgg
     6781 ggtatcccgg tgcagctgcg ttaggggatg ctgtgtgcgg tatggcagtt gcatatatca
     6841 caaaaagaga ctttcttttt tggtttcgat ctcacactgc gaaagaaatc aaatggggag
     6901 gcgctaagca tcatccggag gataaagatg atgggcaacg aatgcatcct cgttcgtcct
     6961 ttcaggcttt tcttgaagtt gttaagagcc ggagtcagcc atgggaaact gcggaaatgg
     7021 atgcgattca ctcgctccag cttattctga gagactcttt taaagaatct ttgcggccgc
     7081 aacctaagaa aaagagaaag gtgggcggag gcatgaagct actgtcttct atcgaacaag
     7141 catgcgatat ttgccgactt aaaaagctca agtgctccaa agaaaaaccg aagtgcgcca
     7201 agtgtctgaa gaacaactgg gagtgtcgct actctcccaa aaccaaaagg tctccgctga
     7261 ctagggcaca tctgacagaa gtggaatcaa ggctagaaag actggaacag ctatttctac
     7321 tgatttttcc tcgagaagac cttgacatga ttttgaaaat ggattcttta caggatataa
     7381 aagcattgtt aacaggatta tttgtacaag ataatgtgaa taaagatgcc gtcacagata
     7441 gattggcttc agtggagact gatatgcctc taacattgag acagcataga ataagtgcga
     7501 catcatcatc ggaagagagt agtaacaaag gtcaaagaca gttgactgta tcgccggaat
     7561 tctgataatt aattaacgat cgagcggccg cgaattcgcc agcacagtgg tcgaccctgt
     7621 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     7681 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     7741 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     7801 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     7861 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     7921 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct taccatgacc gagtacaagc
     7981 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg
     8041 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg
     8101 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
     8161 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     8221 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     8281 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
     8341 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     8401 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
     8461 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
     8521 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgacgcccg ccccacgacc
     8581 cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc gataaaataa aagattttat
     8641 ttagtctcca gaaaaagggg gcggccgctc gagacctaga aaaacatgga gcaatcacaa
     8701 gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg
     8761 aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg
     8821 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     8881 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     8941 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     9001 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     9061 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     9121 atttataact tgcaaagaaa tgaatatcag agagtgagag gccttgacat tataatagat
     9181 ttagcaggaa ttgaactagg agtggagcac acaggcaaag ttctagagct cgctgatcag
     9241 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     9301 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     9361 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     9421 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     9481 cggaaagaac cagctggggg cgcgcccctc gaggccgcca tggtcatagc tgtttgacgt
     9541 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     9601 attcaaatat gtatccgctc acgagacaat aaccctgata aatgcttcaa taatattgaa
     9661 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     9721 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     9781 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     9841 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     9901 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     9961 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
    10021 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
    10081 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
    10141 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
    10201 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
    10261 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
    10321 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
    10381 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
    10441 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
    10501 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
    10561 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
    10621 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
    10681 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
    10741 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
    10801 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
    10861 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
    10921 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
    10981 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
    11041 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
    11101 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
    11161 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
    11221 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
    11281 gcctatggaa aaacgccagc aacgcggcct ttttacggtt ctggcctttt gcacggaccg
    11341 ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt
    11401 cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt ggaagggcta
    11461 attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac acaaggctac
    11521 ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga
    11581 tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag
    11641 aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg
    11701 ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg
    11761 gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt
    11821 ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa
    11881 gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct
    11941 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
    12001 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
    12061 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
    12121 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
    12181 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
    12241 gggtgcgaga gcgtc
//
