LOCUS       sequence-212351-       21224 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 21224)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 21224)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHKU38.2-2.2
FEATURES             Location/Qualifiers
     source          1..21224
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1868..2167
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
     promoter        4899..5119
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             5120..5923
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     terminator      12426..12613
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(12481..12505)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     terminator      15363..15612
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(15379..15397)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(17357..17377)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(17357..17375)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(17714..18302)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(17794..17813)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(18473..19264,19265..19333))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     19096..19115
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(19334..19438)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      19465..20807
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(20428..20475)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     20848..20866
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(21208..21224)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(21208..21224)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 ttaattaata tctaaaaatt gccttatgat ccgtctctcc ggttacagcc tgtgtaactg
       61 attaatcctg cctttctaat caccattcta atgttttaat taagggattt tgtcttcatt
      121 aacggctttc gctcataaaa atgttatgac gttttgcccg caggcgggaa accatccact
      181 tcacgagact gatctcctct gccggaacac cgggcatctc caacttataa gttggagaaa
      241 taagagaatt tcagattgag agaatgaaaa aaaaaaaaaa aaaaaaggca gaggagagca
      301 tagaaatggg gttcactttt tggtaaagct atagcatgcc tatcacatat aaatagagtg
      361 ccagtagcga cttttttcac actcgaaata ctcttactac tgctctcttg ttgtttttat
      421 cacttcttgt ttcttcttgg taaatagaat atcaagctac aaaaagcata caatcaacta
      481 tcaactatta actatatcgt aatacacaat ggaggattcc tttatcctga agaacgtttc
      541 aatatttaca ggtgatgact ggatagattc tggttgcata gcagttagga atggcataat
      601 caactacgtg ggccgcgggg atcctccagc tcgaatagta cccgagggca ctgcagttct
      661 ctcattgccg cggagaagtg tgattcctgg tctcatcgat tcacgagttc attgtcagga
      721 aggtaacatt gaatgtctcg agcagtcgct gagattcggc gtgacgactg tctgcgacat
      781 gtatggcgac cctcagtatc ttaatcttct aacacagcaa gcacaagacc ctgtgaacaa
      841 agacaagtat gccgacttta aatgtgcagg catcgcagct agtatcgaga gcgagtggcc
      901 tggaacggag cttcaattat cccctgagaa caaacctgta agcctagagt tcagagcaga
      961 gagtttaaaa gcagtcactc accaagaaat gaccgcatgg cagtctagtg tcccggagga
     1021 tatcttgccc aaactgaaat ctgctgagga tgcagagcat tacgtccgag aacaagtcgt
     1081 caaattcaac gcatcatata tcaaaatcat gcacgggatc ggcgacgatg tcgacgtgga
     1141 atttacggag ccaagatgcg aagtgcaaaa cgcagtggta gatgcggcgc acaggcgtgg
     1201 tttgatcgtt ttaggacatg cgttgagcta ttccgcggcg atggatctgc tctcaaacgg
     1261 tgttgatggg ttgaccaaca tcttcattga tagatcgaca tctcgcgact ggattgagct
     1321 tctaaagcgc aacaagggac acttgtgccc aactttgtcg ttttatatct ttcgcactct
     1381 ccaaggcgac gatattgaag agctgttcgc aagcgatccg ttctcagagc gtttgctctt
     1441 cgacaaaagt tctcagagga acgtcagtct cggttccaag cctgaagatg cagctttcgc
     1501 caacgctgtg cgcaatgtac aagaggcata caaagccggg gtgccatttg tcatcggatc
     1561 ggccagcaca ggcagagaga ttggcccggc atatgggttg ggtgtccata tcgagatgta
     1621 tctgctttca aaagcagttg gaatgactcc aatggaggtc ttgaagagtg caacaagtga
     1681 agtcgcggat cgttttggat tccatgatcg tggccgtgtt gcagtaggca agaaggcgga
     1741 tctggttctg gttgagggtg acgtccgaga agccattgca gatccaaact gccggtgtct
     1801 cccagtctca tgcgtgtgga gagaaggtgt tagctcgata atttaccaaa gactggctgg
     1861 gccataggcg gatctcttat gtctttacga tttatagttt tcattatcaa gtatgcctat
     1921 attagtatat agcatcttta gatgacagtg ttcgaagttt cacgaataaa agataatatt
     1981 ctactttttg ctcccaccgc gtttgctagc acgagtgaac accatccctc gcctgtgagt
     2041 tgtacccatt cctctaaact gtagacatgg tagcttcagc agtgttcgtt atgtacggca
     2101 tcctccaaca aacagtcggt tatagtttgt cctgctcctc tgaatcgtct ccctcgatat
     2161 ttctcatttt ccttcgcatg ccagcattga aatgatcgaa gttcaatgat gaaacggtaa
     2221 ttcttctgtc atttactcat ctcatctcat caagttatat aattctatac ggatgtaatt
     2281 tttcactttt cgtcttgacg tccaccctat aatttcaatt attgaaccct cacgatccag
     2341 ttctccagtg acacagcctt tatctggtca aacctttctt tctaatcacc tatgctgatg
     2401 cttaattaag ggatttttgt ctccatcaac ggcatgcgcc caaaaatgac gtttttttta
     2461 acccatagac acgaaactac ccattttcca ccggcctgac ctaccaccgg aacaacggcc
     2521 atctccaact tgcaagttgg ggaaattaag agcatcgcag gtttaatgga agaaaaaaaa
     2581 aaggtacagc acagcgcaaa tggagttagt tcccttatgt cacacactca cacacagtcg
     2641 gtcagatcaa gcatactggg tgcgtataaa tagagtggcc attgccaccc tgtttatctc
     2701 aaaatctgtc ttgttagtgg tcttctccct ttttcaggtt acaattctct tgtttctact
     2761 tagtatataa gtatatcaag ctatattaag catactatca actgtcaact ctatcctcaa
     2821 aatacaatac aaaatgacta ctattccaga gaaggcaact gttcttgtga ttggaggcgg
     2881 gccgggaggt tcttacgccg ctacggcact tgcaagagag ggcgttgatg tggttttgct
     2941 tgaggcagac aaatttcctc gttaccacat cggcgagagc atgttggcgt ccatgcgcca
     3001 ttttcttcgc ttcatcgatc ttgactccac attcgacaat catggtttca tcaaaaagcg
     3061 cggggcagcc ttcaagctga atgacaaacc cgaaggcttt accgatttca ttgctgcggg
     3121 aggaccggga aactatgcct ggaatgtgat tcgctcagaa gcggactacc taatgtttca
     3181 gcatgctgga aagagcggcg taaagacatt cgatcaagta gccgtcagat cattggaatt
     3241 cactccatgt gacctcgaac taccggatcc gaaaacacca agccctggca gagcggtcgc
     3301 agcttgctgg tctagcaagg ttgacggtac atctggcaaa atcagtttcg attatttagt
     3361 tgatgcgagt ggtcgtttcg gcataacgag cacgaagtat cttaaaaacc gcaagttcaa
     3421 tcagggattg aagaacatcg ccaactggtc gtattggaag aacgcgggaa gatacgcggt
     3481 tggaacccag agagagggtc agcccttttt tgaggcccta agtgatgcaa gcggctggtg
     3541 ctggttgatt ccactccatg atggtactgc ctctgtcggt attgttcaga atcaggatgc
     3601 ggccgctgcc aagaaaaaag ccatggattc gccatcacaa catgacttct acattgaaag
     3661 cttaaagcta gcgccgagga ttcaggcact gcttgcccaa ggagagatga cggctcctgt
     3721 taaacaagcc tctgactggt cgtatagtgc atcatgctac gcttcgccta acgttcgaat
     3781 tgttggtgat gctgggtgct ttatcgatcc ctatttctcc tctggggtgc accttgcgct
     3841 ttccggtgcc atgtcggcag cttgcacaat tcgtgctgtg cagcgtgaag aatgcgacga
     3901 aaaagctgca atggactggc actcgaagaa ggtggccgaa ggctatacac gcttcctgct
     3961 cgtcgtcctc agtgccatga gacagatccg caagtcagat caaccaatcc tgagcgactg
     4021 ggacgaggaa ggtttcgacc gcgcttttgc attcttccgt ccaatcattc agggaaccgc
     4081 cgacgtcagt aacaagctga gccaagaaga gttggccaag acgattgact tttgtctcaa
     4141 tgctttccaa catcacgacg ccgatgcgag agacgctatg atcaaaaagc tgcaggagca
     4201 cgagcaggaa gctagaatgc gtggagatgt tcaagccaag gcagatattg atccagattc
     4261 actcactcca gaagaattgc aggttttgaa gactattcgc gctcgacaaa tgctgagaac
     4321 cgaagatgtt atcaatatcg attccttcgg aactgacgca attgatgggt tcgctccacg
     4381 ggtgaagcac ggagagctgg gacttatgaa gggtaagaac tccggcccag ctgctcccaa
     4441 gatggatctc tttgcaaagg cgaaagagga gcctgcaatg cagacgcaag tgggtactgc
     4501 agtcacagcg cattgaacaa atcgctctta aatatatacc taaagaacat taaagctata
     4561 ttataagcaa agatacgtaa attttgctta tattattata cacatatcat atttctatat
     4621 ttttaagatt tggttatata atgtacgtaa tgcaaaggaa ataaatttta tacattattg
     4681 aacagcgtcc aagtaactac attatgtgca ctaatagttt agcgtcgtga agactttatt
     4741 gtgtcgcgaa aagtaaaaat tttaaaaatt agagcacctt gaacttgcga aaaaggttct
     4801 catcaactgt ttaaaaggag gatatcaggt cctatttctg acaaacaata tacaaattta
     4861 gtttcaaaga tgaatcagtg cgcgaaggac ataactcatt caattcaatt catcattttt
     4921 tttttattct tttttttgat ttcggtttcc ttgaaatttt tttgattcgg taatctccga
     4981 acagaaggaa gaacgaagga aggagcacag acttagattg gtatatatac gcatatgtag
     5041 tgttgaagaa acatgaaatt gcccagtatt cttaacccaa ctgcacagaa caaaaacctg
     5101 caggaaacga agataaatca tgtcgaaagc tacatataag gaacgtgctg ctactcatcc
     5161 tagtcctgtt gctgccaagc tatttaatat catgcacgaa aagcaaacaa acttgtgtgc
     5221 ttcattggat gttcgtacca ccaaggaatt actggagtta gttgaagcat taggtcccaa
     5281 aatttgttta ctaaaaacac atgtggatat cttgactgat ttttccatgg agggcacagt
     5341 taagccgcta aaggcattat ccgccaagta caatttttta ctcttcgaag acagaaaatt
     5401 tgctgacatt ggtaatacag tcaaattgca gtactctgcg ggtgtataca gaatagcaga
     5461 atgggcagac attacgaatg cacacggtgt ggtgggccca ggtattgtta gcggtttgaa
     5521 gcaggcggca gaagaagtaa caaaggaacc tagaggcctt ttgatgttag cagaattgtc
     5581 atgcaagggc tccctatcta ctggagaata tactaagggt actgttgaca ttgcgaagag
     5641 cgacaaagat tttgttatcg gctttattgc tcaaagagac atgggtggaa gagatgaagg
     5701 ttacgattgg ttgattatga cacccggtgt gggtttagat gacaagggag acgcattggg
     5761 tcaacagtat agaaccgtgg atgatgtggt ctctacagga tctgacatta ttattgttgg
     5821 aagaggacta tttgcaaagg gaagggatgc taaggtagag ggtgaacgtt acagaaaagc
     5881 aggctgggaa gcatatttga gaagatgcgg ccagcaaaac taaaaaactg tattataagt
     5941 aaatgcatgt atactaaact cacaaattag agcttcaatt taattatatc agttattacc
     6001 ctatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggataggaa
     6061 aaaaccgagc ttcctttcat ccggcgcggc tgtgttctac atatcactga agctccgggt
     6121 attttaagtt atacaaggga aagatgccgg ctagactagc aagttttagg ctgcttaaca
     6181 ttatggatag gcggataaag ggcccaaaca ggattgtaaa gcttagacgc ttctggttgg
     6241 acaatggtac gtttgtgtat taagtaaggc ttggctgggg atagcaacat tgggcagagt
     6301 atagaagacc acaaaaaaaa ggtatataag ggcagagaag tctttgtaat gtgtgtaact
     6361 tctcttccat gtgtaatcag tatttctact tacttcttaa atatacagaa gtaagacaga
     6421 taaccaacag cctttcccag atatacatat atatctttat ttcagcttaa acaataatta
     6481 tatttgttta actcaaaaat aaaaaaaaaa aaccaaactc acgcaactaa ttattccata
     6541 ataaaataac aacatgggca atcaaatctc agcattgatg tctgatagca ttgtcttcag
     6601 tcttttcttg cgaatatcat atctaggcct aattctgtcg ataatctatg ttttggcagc
     6661 tttgacctac aacgtttatt ttcacccact caaaaactac cccggaccat tgtcgtgggc
     6721 agcgacgcgt attccctggt gctggtacca atatcacggt gtcaattggc gcaaaattct
     6781 tgagcttcac gcaagatatg gacatgttgt gcgcattggt ccgaacgagc ttagttacac
     6841 ctcctctgag gcttggagga ttatctatgg gcatcgtgtt atcgaaatgg gaaaagatcc
     6901 ggtgttctcg ctgcacactc caactggcgt tcagaacatc ctagtagctg accgtccgac
     6961 gcatacaaga cagcgacgac tacttgctca tgcgttctca gaaaaggcgt tgcgtgagca
     7021 ggaagagatc cttcaatcct atactgatca attgatccgt ggcttagaag cccgccggca
     7081 agatgctgtc gacattggtg catggttcat gtttaccacg ttcgatctga tagccgatct
     7141 gtcatttggc gacaatgtgg gctgcctcga aaagggcgag tatgatccat acgtggcagc
     7201 aacaccagca atttccaagg agttgcagta ctaccagatg ctaaagtact ggggcatcgc
     7261 ctgggctcga aaatggttta tgccaacctc tgttgctgga gcccggatgg ccaatatgaa
     7321 gcgcgccatg agtacggtca acaatcgcat cgaaaaaaat ggttcagtgg atcgaaagga
     7381 ttttttgcac tacattcttg cagcgaatga tgaaaagggg atgtcacgac aagaaattga
     7441 cgttaatgcg ttctcgatca gcactgccgg ttccgaatca acggccacag ccctttcagg
     7501 tgcaatattt tacctcttgc aacacccggc ttcttatgcg agcgccacgc gtgaagtccg
     7561 ctcagcgttc catgcacctg aggagatcac catgagcgaa gtcaagaagt tgaagtactt
     7621 gaaagcagtt ttacaagaaa ctctgcgcct gtacccaccc gttgctgtta ctttgccccg
     7681 tcgcgttcca gttgaagagg acggagtatt cattgcaggt catcctgttc cagccggaac
     7741 aacagttggt gtcaactatt atgctgcatt ccattcacct tacaactttc accttgccga
     7801 gaagtttgct cctgaacgat ggcttccaga gcactcagat catgcgccct acgcaaatga
     7861 caatagagat gtagtccagc cattcagtta cggaccgcgc aattgtttag ggaagaatct
     7921 cgcgtgggcc gagatgcagc taattcttgc aagggtgtta tggcattatg atattacatg
     7981 gcccaaagac aagcttggaa ggccagaagt tgatgaaggc tggcagttaa accaaaaact
     8041 gtacggcttc tgggtcaaac caccactaag ggtttgcttg aagccggccg atggaatcag
     8101 gaaatgaagt gcttttaact aagaattatt agtcttttct gcttattttt tcatcatagt
     8161 ttagaacact ttatattaac gaatagttta tgaatctatt taggtttaaa aattgataca
     8221 gttttataag ttactttttc aaagactcgt gctgtctatt gcataatgca ctggaaggag
     8281 aaaaaaaagg tgcacacgcg tggctttttc ttgaatttgc agtttgaaaa ataactacat
     8341 ggatgataag aaaacatgga gtacagtcac tttgagaacc ttcaatcagc tggtaacgtc
     8401 ttcgttaatt ggatactcaa aaaagatgga tagcatgaat cacaagatgg aaggaaatgc
     8461 gggccacgac cacagtgata tgcatatggg agatggagat gatacctcca ttgggccgat
     8521 gaagttagtc gacggataga agcggttgtc ccctttcccg gcgagccggc agtcgggccg
     8581 aggttcggat aaattttgta ttgtgttttg attctgtcat gagtattact tatgttctct
     8641 ttaggtaacc ccaggttaat caatcacagt ttcataccgg ctagtattca aattatgact
     8701 tttcttctgc agtgtcagcc ttacgacgat tatctatgag ctttgaatat agtttgccgt
     8761 gattcgtatc tttaattgga taataaaatg cgaaggatcg atgaccctta ttattatttt
     8821 tctacactgg ctaccgattt aactcatctt cttgaaagta tataagtaac agtaaaatat
     8881 accgtacttc tgctaatgtt atttgtccct tatttttctt ttcttgtctt atgctatagt
     8941 acctaagaat aacgactatt gttttgaact aaacaaagta gtaaaagcac ataaaagaat
     9001 taagaaaatg gcacattctc aggattttga agttcttatc attggagccg gcatcaccgg
     9061 cctcgtgctt gcacaagctc tgaagaaggt tggaataaag tacaaaatct tcgaacgaga
     9121 ggtacagatg aactatcgga gcaacgagtg gacgatggca atccattggt cgcttgagcg
     9181 cctggaaaat ctccttccaa aggagatctg ggagaaactg cccacgatat cctgcaatcc
     9241 agctattcct atggatgctg gaggcaagta tcccatcatt catggtgaaa ccggcaagct
     9301 cctggccggt gtgccatatg ccaggggctt gcgtgtccca cgcagcaaaa tgagagctct
     9361 gtgtgctgaa ggaatcgatg tgcagtacgg catgaagctt actggagtcg atttcgatgc
     9421 ctctggagtt ggcgtaacgg ctacctttaa tgactatgag aaagtcaaag cgtccctcat
     9481 cattggcact gacggcccaa ggtctacgat gcgcattttt gccatgggaa gcgaggcaga
     9541 ggcgtctact agcaaatttc caatctttca tacgaacatg acagtttgct acggcgacgc
     9601 tgaaaaggca aagtatttgc gccaggaatt tcccacgagt tatctcgctc tcagcgaaaa
     9661 tagctaccac gctttccagt caatttccag tatgcctgat ggacctgagc atcctgaaac
     9721 ctgggtgttc catctggcca tggcttggct cggggactct gatcagtcca tgagctatcc
     9781 tgaaagactc gctttgatca aaagcaaggc tcagaccctt ggtgagcctg caagatctgc
     9841 ctttacatgg ataccagaga acacgcttgt tcacaaggcg gacatcagtt actggattag
     9901 caaaccgtgg aataatcacg acggtagaat gaccttggct ggcgatgcag cccacgccat
     9961 gccaccttac cgcgggcaag gtctaaacca ttgcatctgc gatgtgtctc accttcttga
    10021 aaagatccaa agcatcttga ataatgagat gggtcaaaag gaagccatct ctgcctatga
    10081 tgacgaggtc gttaaaaggg gtgctgagga ggtaaaatgc tcgctggaga atgctctcat
    10141 gctgcacgac tgggagaaag tgcagagatc acctgtgttt acaaccggct tcaagccaat
    10201 gactgggcat gacggggaga actctcatca aagaatgctt cccgaagcct tgaagactct
    10261 tcaagaagtc aagcaacaga ttgctagtca ttagatttaa ctccttaagt tactttaatg
    10321 atttagtttt tattattaat aattcatgct catgacatct catatacacg tttataaaac
    10381 ttaaatagat tgaaaatgta ttaaagattc ctcagggatt cgattttttt ggaagttttt
    10441 gttttttttt ccttgagatg ctgtagtatt tgggaacaat tatacaatcg aaagatatat
    10501 gcttacattc gaccgtttta gccgtgatca ttatcctata gtaacataac ctgaagcata
    10561 actgacacta ctatcatcaa tacttgtcac atgagaactc tgtgaataat taggccactg
    10621 aaatttgatg cctgaaggac cggcatcacg gattttcgat aaagcactta gtatcacact
    10681 aattggcttt tcgctagtct tatctaaaaa ttgcctttat agtccgtctc tccagtcacg
    10741 gcctgtgtaa ctgattaatc ctgcctttct aatcaccatt ctactgttta attaagggat
    10801 tttgtcttca tcaacggctt ccgcccaaaa aaaagtatga cgttttgccc gcaggcgtga
    10861 agctgcccat cttcacgggc ctgacctcct ctgccggaac accggccatc tccaactcat
    10921 aaattggaga aataagagaa tttcagattt tcagaggatg aaaaaaaaaa ggtagagagc
    10981 ataaaaatgg ggttcacttt ttggcaaagt tacagtatgc ttattacata taaatagagt
    11041 gccgataatg gctttttttc atcttcgaaa tacgcttgct actgctcttc cagcgttttt
    11101 attacttctt tcttgtttct ccttagtata taaaatatca agctacaaca agcatcaatc
    11161 actgtcaact gtcaattata ttataataca ctatgcccgc ctccaaattg gcacaagagt
    11221 ctactcaaaa gccagaattc aggccgaccg tttatggact gatttctgag ctttgcgtgt
    11281 tgctgaataa gctagagagt gatcgtgcgg aggtgccctc ccttgcagcg ggcgcgcatt
    11341 ccaagctgtg gactgcccaa ggcgatagct tagcggcgtc tcgcgatcga gctttggagc
    11401 ttttgaggaa cctatgccag ctattgcaag gaccgcatga atactttcat tccttggtgt
    11461 cttcaaattg ggatcttgga gctctctaca caatcatcga gcttggaata ttggagaaaa
    11521 tccctttgac ctcttgcgca aacgtgattg atctttctga agagcttggc ttagatgagc
    11581 ggaaattgct tacactcctc cgacttgtca catgctcaaa cattctttac gaggccgaag
    11641 acgggttctt tgggcatact gccctgtctc aagagctttt gcaagacgag caattccgag
    11701 ccttcatagg attccagctg tttgaggcca ggatcggcag tgtgcatttg gcagactccc
    11761 tgaaggggag caaaaacacc tttgagcatg ggtgttcagc tttcaaacac gcaatgggta
    11821 taccgctata ccagtggcat ggtgagaaca cggagaaagg tacgaggttc cgtatggcta
    11881 tgcagggggt ctcaaaaagt ctagaccctg gtgacgattt gttccgtcag tggtttaagc
    11941 agcattctga atctggcaca tgtctaaccg tcgtcgacgt tcttggaggc aacaaaccaa
    12001 gccagcaaaa gttcattggc gagtttccag agctcaattt cgtttccgtt cacgacattg
    12061 gcgaaatagc ctttgttgac ggacctattc tggcatatgt cgtccgcaat gtgttctgga
    12121 acaagagaga caacgaggtg atctccgtga ttcggagcat ccaccaagct atggagaaaa
    12181 acgcaaaagc cacgctgttg attaatgaaa tgctgtcgcc agctagaggc gaattcccag
    12241 gagatctcga gcacgcctat cgtcgacgcg acgttacaac gatgactatg cacaacgcga
    12301 agcaacggac tgtcgctgaa tggcatgggc tatttcgtga agtagatcta aagtggaaga
    12361 tccgactggt cacgggtaat tcgtcacata gctacagagg gctttgggag atacaaatga
    12421 attaggcgaa tttcttatga tttatgattt ttattattaa ataagttata aaaaaaataa
    12481 gtgtatacaa attttaaagt gactcttagg ttttaaaacg aaaattctta ttcttgagta
    12541 actctttcct gtaggtcagg ttgctttctc aggtatagca tgaggtcgct cttattgacc
    12601 acacctctac cggcatgcct ctcaaatctt ttagcgccaa ggactccaac taattgtatc
    12661 ttgaatttgc ctttacgatc cgtttgtcca gtcacggcat gtatatctta ttaatcctgc
    12721 ctttctaatc acgtattcta atgttcaatt aagggatttt atcttcatca acggctccca
    12781 cgcaaaaaat gacgttttgc acacagacac gaaatacacc ttccaccgga acaacggcca
    12841 tctccaactt ataagttggg gaaataagac aatttcagac ttcagagaat gaaaaaaaaa
    12901 aaaggtacat cacagatggg gttcaggttt gctacaattg cagggagcct gtcacatata
    12961 aatagacctc cagtgatgat atctttcagt cttcaaacgt ctcttgtcac agttctggtc
    13021 gttctatatc acatctctct tggttctact tattgtctat aatatcaagc tacagcaagc
    13081 atacaatcaa ctatctacca taccataata cacaatgata attctggggc ttccgaccgc
    13141 gcttgtgcac tttgtcggga gaaaaaaatc cgttgtggcc gagagaaacc caaatgtggc
    13201 aattgtaaac gtcagaaatg gcgctgtgac tactctgcgc cagaaaagcg taagtgaaaa
    13261 tgtcaactct atcgatgctc gattgggcaa cattgagaac gaaatctttc gtcttataag
    13321 atcccaaaac gcgcccaggt ctcgacatgg ctcggagggc acgcctacat cgtatgagag
    13381 tccgttcaat ccaagtgaca ccgaagatga gtcgcggggc agcctgttga caacagaaag
    13441 acaactcttg cactcgccag tcacggattc atactacgga ccggatacgt tttatgcatg
    13501 ggccactgcc ttcaaagggc tagtgacatc tcggcgacaa aaagtaggaa aggatgtctc
    13561 tcaacaaggg ctgaattttg cggttgatga tgagctgggc tccatcgacc atgatttatc
    13621 ggaattagta cgatatgccg agagacagga cacgctgtac gactcgagaa tcaccgaaac
    13681 ctcaatcatg ttgccaccca agcagttcct gccgatgttt catacaagat tctttcacca
    13741 caacgattgg tcaacagatc tatttgttca agaaaggttt tggcagaacg ttgatcgagt
    13801 gtatgccaat tacaactcta ctctagacga accatgggca attgttttca atacaattat
    13861 actactttct atgagcgcag aaaactctct ccagactcat gctcccttgg caacttcgca
    13921 atttgctgcc cccttctttt cagcgctcaa agttgccctc aataaagtcc atttattaat
    13981 ggcgccgcga ctcataaacg tacaagcctt ggcccttcta agcttagctg ccgagcaatt
    14041 tcatccgttc ggagttgcag aagcagtgtt tgtgcaagcc tgtaatctgg caagacgaat
    14101 gggtcttaat cagattgcga tgactggaac atcggatgca attgaagagg aagagcgtct
    14161 caaagtctca agggctttat acattcgtga caaaagcctg ggtatttcac gaggcaccat
    14221 atgctggctg ccagggtttg aagtgcaaaa tatgaaactg gacgactttg aatcagccga
    14281 aggaagatac catgctgcac gagtcacgat tactgctttt caggagcgcg tgtatcgcct
    14341 ccattcttca tctggggctc aagatgactt tgctaacaaa gcgggcttcc atccctctgg
    14401 tatacaacac ggtttggccg atcttctgga aattggcacg atgtgcggtc cccaccttga
    14461 actttggtta gaattcctcg ctacccgtat tgttgctcta cgaaggaaca ctaaagcgca
    14521 ttacgtcaag cgcactttaa aagattgtag agcatgttgc atcctgctac tattggcatt
    14581 cggaaagggt gacaaagcac tgagggaacg actcgagcac cattgcgacc tccatacaac
    14641 ttctcaacaa ggttccaaat catcctctct agactctgaa atcagcctca atgagcacgc
    14701 aatatcgacg ccaagaactc tcctctcact cttcccggtg tctagcttat ttctgttggc
    14761 tgcaaacgtg atcaaccctg caactccaga ggaggaagcg aacactgatg atgactacga
    14821 gcttctcgtc agagtttgcg attgctacga ggaagctgat gcaagcctac aagccatcaa
    14881 cagggcccat caagttggta aggcgctcca aagaattctg tccatcattc aacacgtgcg
    14941 tgggatgaat ggcacttccc ctagcaaaag cacgagtggt tcgggtagca gccgatgtca
    15001 ctccgcgtcg ttgtcatcca gtcagctgga acaaggcggt tcttttccag atcagtcgag
    15061 tttgcagcat ctgccaagtt ctggtttcat aaactcgcag catttcacag atggggcgcc
    15121 gctatcttca ctctgtgtga acgatattga gacattcgta atgaacacgc catcaggaca
    15181 tgagataagc tcggcgttct ccgtggaaaa tgatatgatg agcttcatga ggcaagatgc
    15241 tatgacttcg aatcatttca gatcggacaa ttacggaatg gaaatttgtt atccagtgga
    15301 gggtgcggac ccaaatggcg ggttgccggg aatatgaaca ggcccctttt cctttgtcga
    15361 tatcatgtaa ttagttatgt cacgcttaca ttcacgccct cctcccacat ccgctctaac
    15421 cgaaaaggaa ggagttagac aacctgaagt ctaggtccct atttattttt tttaatagtt
    15481 atgttagtat taagaacgtt atttatattt caaatttttc ttttttttct gtacaaacgc
    15541 gtgtacgcat gtaacattat actgaaaacc ttgcttgaga aggttttggg acgctcgaag
    15601 gctttaattt gcatttattg aaaagtaaat atctcgtaac ccggatgctt tgggcggtcg
    15661 ggttttgcta ctcgtcatcc gatgagaaaa actgttccct tttgccccag gtttccattc
    15721 atccgagcga tcacttatct gacttcgtca ctttttcatt tcatccgaaa caatcaaaac
    15781 tgaagccaat caccacaaaa ttaacactca acgtcatctt tcactaccct ttacagaaga
    15841 aaatatccat agtccggact agcatcccag tatgtgactc aatattggtg caaaagagaa
    15901 aagcataagt cagtccaaag tccgccctta accaggcaca tcggaattca caaaacgttt
    15961 ctttattata taaaggagct gcttcactgg caaaattctt attatttgtc ttggcttgct
    16021 aatttcatct tatccttttt ttcttttcac acccaaatac ctaacaattg agagaaaact
    16081 cttagcataa cataacaaaa agtcaacgaa aaatggacgc ttaccatctg gatcctccga
    16141 aagttgcatc agcgcaaacg gcggcatctc ctgaagagaa ggccatacca cagcccaggc
    16201 cgggacctac tacgactccc ccagttcttg atacggcaac actcaagtac atcaaaacac
    16261 taagtatcaa acatttccgt tctgcagatg tcccatggcg ctccatgggt cagtacaagt
    16321 accgcattat cgaggatggc actcttaccc aagaccgcat atccgtggta gagtcgctta
    16381 tcccgccaaa atcacacggt cctatttttc atttccatga gatgcacgac gaatgcttct
    16441 acgtcacaca aggcactgtg cgttttcatt cgccaggaag cccgccagtg gacgctcatg
    16501 tgggggacat ggtaacagtg ccaacgagga tgcctcatcg attcagcaac ccatttgatg
    16561 aggaggccaa atttgtatca acaaacacac cgagtcacta tgcacgttac ttctactacc
    16621 tggaatacct gattggtgag ggaaatgagc tgacaccaga agtgaataaa gaagcaatgc
    16681 gtcggttcgc tacggtgcca ttatcgcccg cggacattga attgttggaa agagaattgg
    16741 gcgccggcac agttggaaat gagaacgagt acaattcaga cgagtcagta gatacggcgg
    16801 acttggtcag tcttgattag ggagattgat aagacttttc tagttgcata tcttttatat
    16861 ttaaatctta tctattagtt aattttttgt aatttatcct tatatatagt ctggttattc
    16921 taaaatatca tttcagtatc taaaaattcc cctctttttt cagttatatc ttaacaggcg
    16981 acagtccaaa tgttgattta tcccagtccg attcatcagg gttgtgaagc attttgtcaa
    17041 tggtcgaaat cacatcagta atagtgcctc ttacttgcct catagaattt ctttctctta
    17101 acgtcaccgt ttggtctttt atagtttcga aatctatggt gataccaaat ggtgttccca
    17161 attcatcgtt acgggcgtat tttttaccaa ttgaagtatt ggaatcgtca attttaaagt
    17221 atatctctct tttacgtaaa gcctgcgaga tcctcttaag tatagcgggg aagccatcgt
    17281 tattcgatat tgtcgtaaca aatactttga tcggcgctat gcggccgcca ccgcggtgga
    17341 gctccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgaga ctcaaacaag
    17401 atatattttg cccgccttac agacgaaact agttggaggt tatggagcat actatcacgt
    17461 gggcggccac tggtgagtta ctacacccca ggggcaacgt tgatgctcct aaaaaactct
    17521 ggctggacgc aagccgtaac acccgtgtca cttcataatc gtttgcaatt cagggcttga
    17581 tctacacatt accctgttat ccctattaat taatacggtt atccacagaa tcaggggata
    17641 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
    17701 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
    17761 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
    17821 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
    17881 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
    17941 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
    18001 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
    18061 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
    18121 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
    18181 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
    18241 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
    18301 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
    18361 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
    18421 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
    18481 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
    18541 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
    18601 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
    18661 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
    18721 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
    18781 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
    18841 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
    18901 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
    18961 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
    19021 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
    19081 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
    19141 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
    19201 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
    19261 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
    19321 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
    19381 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    19441 catttccccg aaaagtgcca cctgaacgaa gcatctgtgc ttcattttgt agaacaaaaa
    19501 tgcaacgcga gagcgctaat ttttcaaaca aagaatctga gctgcatttt tacagaacag
    19561 aaatgcaacg cgaaagcgct attttaccaa cgaagaatct gtgcttcatt tttgtaaaac
    19621 aaaaatgcaa cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc atttttacag
    19681 aacagaaatg caacgcgaga gcgctatttt accaacaaag aatctatact tcttttttgt
    19741 tctacaaaaa tgcatcccga gagcgctatt tttctaacaa agcatcttag attacttttt
    19801 ttctcctttg tgcgctctat aatgcagtct cttgataact ttttgcactg taggtccgtt
    19861 aaggttagaa gaaggctact ttggtgtcta ttttctcttc cataaaaaaa gcctgactcc
    19921 acttcccgcg tttactgatt actagcgaag ctgcgggtgc attttttcaa gataaaggca
    19981 tccccgatta tattctatac cgatgtggat tgcgcatact ttgtgaacag aaagtgatag
    20041 cgttgatgat tcttcattgg tcagaaaatt atgaacggtt tcttctattt tgtctctata
    20101 tactacgtat aggaaatgtt tacattttcg tattgttttc gattcactct atgaatagtt
    20161 cttactacaa tttttttgtc taaagagtaa tactagagat aaacataaaa aatgtagagg
    20221 tcgagtttag atgcaagttc aaggagcgaa aggtggatgg gtaggttata tagggatata
    20281 gcacagagat atatagcaaa gagatacttt tgagcaatgt ttgtggaagc ggtattcgca
    20341 atattttagt agctcgttac agtccggtgc gtttttggtt ttttgaaagt gcgtcttcag
    20401 agcgcttttg gttttcaaaa gcgctctgaa gttcctatac tttctagaga ataggaactt
    20461 cggaatagga acttcaaagc gtttccgaaa acgagcgctt ccgaaaatgc aacgcgagct
    20521 gcgcacatac agctcactgt tcacgtcgca cctatatctg cgtgttgcct gtatatatat
    20581 atacatgaga agaacggcat agtgcgtgtt tatgcttaaa tgcgtactta tatgcgtcta
    20641 tttatgtagg atgaaaggta gtctagtacc tcctgtgata ttatcccatt ccatgcgggg
    20701 tatcgtatgc ttccttcagc actacccttt agctgttcta tatgctgcca ctcctcaatt
    20761 ggattagtct catccttcaa tgctatcatt tcctttgata ttggatcata ctaagaaacc
    20821 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg
    20881 cgtttcggtg atgacggtga gcgcgtttcg gtgatgacgg tgaaaacctc ggcgcgccat
    20941 taccctgtta tccctaatat acaacgcaaa gaagctggaa aattggcgag ggaatcctgt
    21001 ttctgtctat ccaagaatgg gcatgaggtg gcaaccgtcg tgctagcgta cagggtgcac
    21061 tttgtaacca tttgggacac cggacactcg ctgttttcga aattaccctt taagcgcggg
    21121 tattgaacca ggcttatgcc caagatcgta gcaagcattt aaatgacggt atcgataagc
    21181 ttgatatcga attcctgcag cccgggggat ccactagttc taga
//
