LOCUS       sequence-212366-       14605 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14605)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 14605)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHKU43.1-2.2
FEATURES             Location/Qualifiers
     source          1..14605
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             511..534
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(9036..9056)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9036..9054)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(9393..9981)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9473..9492)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10152..10943,10944..11012))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10775..10794
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11013..11117)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      11144..12486
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(12107..12154)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     12527..12545
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        12840..13247
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             13248..14342
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(14589..14605)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14589..14605)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 ttaattaata tctaaaaatt gccttatgat ccgtctctcc ggttacagcc tgtgtaactg
       61 attaatcctg cctttctaat caccattcta atgttttaat taagggattt tgtcttcatt
      121 aacggctttc gctcataaaa atgttatgac gttttgcccg caggcgggaa accatccact
      181 tcacgagact gatctcctct gccggaacac cgggcatctc caacttataa gttggagaaa
      241 taagagaatt tcagattgag agaatgaaaa aaaaaaaaaa aaaaaggcag aggagagcat
      301 agaaatgggg ttcacttttt ggtaaagcta tagcatgcct atcacatata aatagagtgc
      361 cagtagcgac ttttttcaca ctcgaaatac tcttactact gctctcttgt tgtttttatc
      421 acttcttgtt tcttcttggt aaatagaata tcaagctaca aaaagcatac aatcaactat
      481 caactattaa ctatatcgta atacacaatg gattataagg atgatgatga taagactagt
      541 atggcacaca ggcaacctct agacaatgtt gctcgggatg cccccagcta cacaaatcca
      601 gaccctcgtg ccgtagctat cgtcggcatg tctatcagcg cgcccggcgg tgttgactat
      661 ggtcttgata ccgaagaatt ctatgctttc ctccaaaata gaggatctgc catcattacc
      721 gttcctacta accgctggaa cgcggaagcc tttcatggca ctgggccggg caagatctct
      781 actacaaagg gagggtttat tcctgacatt agatatgccg acctccaaga gttcggtatt
      841 actcctgtgg aaggccaaca gatatctact tgccaaatgc ttctgctcca tcaagcgttc
      901 acggcgttgc agcgcagtgg agttgattat cgtggtacaa acactggggt ttacgttggc
      961 tccgcatccg gcgaaggaac tttgtttgaa atggacatag cacaggcagg cccgtattac
     1021 atgactggaa caaccaactc gatcgttgcg aacagactga actacgtatt tgatctactc
     1081 ggtccttcca tgcccataga tactgcgtgt tcgtcctcgc tggcagcgat gcacgtcgcc
     1141 gttcaagccg tccgcaacgg tgaatgtgac caggctgtag tggccggtgc caacgtcgtt
     1201 cgaaacgcac tcgaatgcgt agcgttcagt cagttaggtg tgctcagccc tgacggcata
     1261 tccaagtcct ttgatgaaga tgggaatgga tacgcacgtg gtgacgtcgc cagcgcggtt
     1321 gttattaagc gccatgatct cgccgtcaaa gatcacaatc gcattcacgc gacgcttgtt
     1381 ggtaccgccc tgacatcatg cggatccatc atgggttccc tcacaacccc gagtcccgaa
     1441 gcgcaggctt cggcgatcca gcttgcatat cgagacgccg gtcttgagcc tcatcaagca
     1501 gatttcgtcg agctccatgg cacgggcacg gttgtcggtg acgcgacaga agcgaataat
     1561 gccggcgccg tctttgcggg agggagagat gcaagggaga tcctcattgg atcggtgaag
     1621 agcaacgttg gacatggtga actaggcgcg tatatgacat cattagtgaa ggtcgcaatg
     1681 atgttggaaa ggaaggaagt cctacctaat ggctacttca aaaagccatc ctcccgaatt
     1741 gatttcgaaa agttcaagct gcgtgttccc gtgtccgttg agaatttcgc acctggcaat
     1801 ccccaactcg gtcggattgc gtctatatct agttacggat ttggaggcgc tctgtcgctg
     1861 aagggatgcg ttactctcag cgagaaatac aaagaagagt gcgctgaagt tgagcctctc
     1921 gccctgtgcg agcacttggc aaaccgtaca cgccaactcc catggaggtc cttcgcaatc
     1981 gctggatcca tcgagacagc gacgttcccc gaaccagtcc tcgtacctaa acgcccccat
     2041 cctctcgtct tttgtttctc aggccagggc ccccaacact ggcagcaagg ccgggagcta
     2101 tatcatactt tcaaggtgtt ccgcgatagt atcaatgact gcgacagggt gcatgtcgat
     2161 tacacagggg aatccttcat cgagacgaca ggcctgttca agaccgatgc tcctaaggac
     2221 tcggacctgg ctaccagctt aacatggcct tccgatgtca cttccatttc tctcaccttc
     2281 ttccagattg cgctcttcga cctcttgagt tctctgaatc ttaagccaga tacaattgtt
     2341 ggacactcag ttggagagac agcagtctta tacgcttcag gcgcggtccc acgtagcatg
     2401 gccgttaaaa tcgcgatcgc tcgtggaaaa gctcttgcca tggttgacaa cactggcggc
     2461 ggtatggtgg ctgtttctgg atgcgacgca gacaaagtac gcgactacgc ggaaacggca
     2521 attacactag ctgtgcagga agtcgactcg gcgggtggtc cccagctgtt ccttgcgtca
     2581 ttcaacagcc ccacagatat aggcgtatca ggctccaaga aacatcttga ggtatttgca
     2641 agcttcatcg aaagatgggt gagcgatgtc gtcgcgcgca aacttcgcat caacaccgct
     2701 gtccactctc ccttcgtcca tccctgcgag tcgcaatacc gccgcgacct agctaccatt
     2761 ttcgcggagt atccaggtcc tcacatccca aagatcccga cgatgtccac ggtcactgct
     2821 gagttcaaaa cagactcgta tacgcccgat tatttgtgga acaacctccg ccaacctgtg
     2881 cttttctctt cggctatccc caaagtagtt gagagactcg gagaattgac cacgtttatt
     2941 gaggtcgccc ctcatccagt cctgtcccag tacctcaagc agatgggtgc ccttgatgct
     3001 ctcgcctgca gcagacgtcc accgtctgcc cgccatgtaa aacctgggtc gagcgctttg
     3061 gtcgaagtac gcacattgct ggaagttttg ggacgtttgc tgttagtcgg cgtcaataag
     3121 atcaacttct cggagtttgc cggatgccca gatgcaaaca tcgagggtcc cgcatatccg
     3181 ttccagccaa agttatggcg ctatggcatg gatgaaccat catacgtccg taagctatta
     3241 cccgcggaaa ggtctttgaa ttcgattcgg ctacgcgtca gcccaattat gcccgagccg
     3301 tggatgggtg atcatgtcat tgatcatacg aatctggttc ccgcggctgc ctacctagag
     3361 atggcccttg agttccccga tgttactcag ctgtatgatt gccgcttcga ggctatgtat
     3421 gtcctggaag agtcaggacc acctgggaca ttggaagttt cccgggaagg gaattcctgg
     3481 tgggtgaagt catcatctca gatgcgcact ccgcaggggg acaaggagtg gacgaggact
     3541 ggtccttctt tcgacactct ccactcttgc ggcaagttgg gttatggtcc ccctgaaatc
     3601 gagccggatg cgatcacacg cgtcgatctt gaggaagtgc tgtctcgctg cccctacacg
     3661 ttcgatcgcg acgcctttaa cgatcggatg gagggtgtgg tacaatttgg tcccgagttc
     3721 agacgaatta tgaaagcggt gtgcaatgaa aaggaagtcc tgacatatat caagggtcat
     3781 gtggatggcc tgaatcagac agattatgcc ttccatcccg ccctcttaga tgccgtcttc
     3841 cagtcgcctc tttcatgggg cctgatcggt gataagctgc atctcggtag gaagaatacc
     3901 cagctactgc tgccacattc tgttcgccgc atattccgca atgatggcag tactgctcca
     3961 ctggtgctgc ctgatgaatt tgtagcttat accgtcctcc acgagtggtc gcctcaccac
     4021 tgggtgacga actgttatat cttaggtgaa gaccagtcgg tcatatatac cgtggaagga
     4081 cttcgttttg cgttggtcca gcatgacaat gagtggccta gcgaacactt caagatgttc
     4141 tggcaaccta gggacttgcc acagtcgcat ctggaaagct cagtcattct tcctggtcca
     4201 ccaccgcatt ccgatgatat agagttattt ggtctacctc acgacttcgc tcctacctcg
     4261 cctgaccgcc agcgctactt ggcttgggca aatgcggtgg ccaccagact gaccgcgaac
     4321 aacgctgatg tgccagcagt ccctgctcat ttgcaagaaa agtataaagc tttgttcgac
     4381 ctgactcaga gggtcggtgc tggacaaaaa gctgccgccg ttgagctgct tttctatgac
     4441 gacatcatga gcaagatcta cgagcatcca cctttcatcg gctctgtatt cgatgcgacc
     4501 acgtcgcgtt tcatcgacct tgttaccgac gcactcaatg ctggcaaaca ggtggtccga
     4561 gtccttgagg ttggtgcggg aactgggagg ttcactgcac tgctcgggcg agctcttttg
     4621 gaagctgctt tgcctgaagg ctgctacgtc gactacgtct gcaccgacgt cagtatttcg
     4681 ctcgcacaag aggcgacagc gaagtcccct tggccaacgg taaccgccaa agcccttgat
     4741 ctgaatgtcg cgatcagcga acagaaccta gatccccgca gtttcgatat tgtcgttgcc
     4801 tttgatgttc tgcacgctat ccctgacatt cacgcgagtt tgacgacgct ccagcagctc
     4861 ctctgtcccg gcggtcacat tgccatcttg gagttggacg gtggttccaa acctgtactg
     4921 ctccgacact tcaccgctgg aggggaagtt gcccttgtcg acttcatatc atcgctggat
     4981 tcaacatacc cctacctctt ctggctatac accactactt cccccggcga tgcagctgcg
     5041 gttggactag tgagaactct ccgccaggaa tacccaacct ggaagattca cctcgctctc
     5101 ttccccgact cctggacggt gacagcccaa gaggcttatg tgcactcaca gctcatactg
     5161 ttgccgtggg tggactctga agtgatgatt gaccaggacg ggaagatgca cgtgccgcgt
     5221 ctggtgaagg cctctcccgc cgctcgccag gaacttcggg gctctcatcc agtctcattc
     5281 cggggttccg atgtctggcg agcgtaccca gcggagcttg gtccgaatga tgttgaagtg
     5341 tccgttcact atgttggatt gactccggct atgtctggat ggaccgaatt tgctggagcg
     5401 gtgacagccg tcggctctgg tgtacttgaa gaggacctcg tcgggcgcag ggtatttggt
     5461 gttactgttt ctgtccccgg aagtaccatc gtctgcgatc gttccaagct tgcgttcgtt
     5521 cctcaagaca tggaaactgc cctggccacc gctttggtgg gaaggcttat gttcttatca
     5581 ttggtcgtgg tcgataccgt cggcacctct tcgaagaaca caaaggtcct cctccacgct
     5641 ggccattgtt caccggccgc gctcgcgaca tacgcttacc taagtgccaa ctccttcaac
     5701 gttactgtca ccgtcagcga tgcaccaccg tccaccaaca ccttcagcac gctgtccccg
     5761 atttcatctt ccaaatacta tgcctggtca tctcgtgtcc gcgcttgggc tccacgaggc
     5821 gtagacgtcg tcttcaattt tgacgaggat atcaacgtgg cgaagcaaac gctttccttg
     5881 ctttcttctc gggggagatt tgttcaaata gggggagagc tgcccagctc tcttccttcc
     5941 ggacgtcgct acgtatcaat cgaagagcat gctgttctag aagctggcaa tgatctcgat
     6001 cgcaccgacg ctgttttttc acccgctgtc cttgctgccc tacctcctgc cgtggacatt
     6061 tacaacctcc cacagctcat gaccgccgac gtccaagcac gcaactcgat gccaggccgt
     6121 gttactctgg tcgacctgca agcgattccc caggagcttc ccgttttgaa agccggaatg
     6181 ttgaaaggga cggctgtttt caatcctcga gcatcctatg tgattgtggg tggtattggc
     6241 ggactgggtg tcaacattgc acggtgcttg gtggaaaatg gtgccaagca cctgattctg
     6301 acttcgagat ctggggagaa gggaatcgcg actatgcaac tagcacgcga gaaaaagatt
     6361 gtcaattatc ttcgaggaat tcctggagta accgtcgatc ttcgggcggt ggacgttctt
     6421 gacgctgaga agaccagaga cttgttcatg aacctggagc atcctgttgc cggggtgttc
     6481 tatttggctg tcaggctcaa cgaccaggtc tttgcgaatt tgaagacgga ggaagactgg
     6541 aaaattggca agtcggctat cttgcgcttt atttacgacg tcaagatcaa aggcgttgaa
     6601 gttctcctgc aagcggtcaa ccctcagtca ctggatttcc ttgtcctcac gagttccatg
     6661 gctactgtgt gcggctcgcc aggccaggcg aactattctg ctgcccagac ctacatggag
     6721 actatggcgg cagcgcttcc caataccgtt gcaatcaccg tgccccctat caccgatgga
     6781 ggtgtgtttg tccgaagcct gcctccaggt aacgcacgca atgcagccct ggacaaatac
     6841 aaatccctcg gaatgactgg ctaccttgtt gcgcaacact gcgtgaatgc catctggacg
     6901 ctgaactgcg atgcctatag cccagtctat attcctccca ccgactggaa agagatcatg
     6961 caacttggca tcccggaata ccacttgtcg tccctgcgtc atttgttgac caagcaaaac
     7021 gttgagatcg cgctggcaac cgaggagtgg tccattcggg cggcgtgcgc atcggtgttg
     7081 gctcttgata ccaacgaggt tgcagacaat gtcccgcttt caagctatgg tcttgactcg
     7141 ctaacatccg ttcgattgag tgggattctc aaaaccaact tcaatgtttc cgtgactcag
     7201 cttcagcttc tcggaaacag tatgactgtc caacgactga tggctctgca agaggagctg
     7261 aaggccgatt catcttctgt ggctgtgggg actgccaaca aggactcgct cgacatactg
     7321 ggagccgaag gtgtccatca tgctgacatg gacaagacga tcgttcgatt gaacaacgtc
     7381 acggatggac aacctgtatt catcgtacac ggcgctggcg gcggcgtcct cgtcatgctg
     7441 aaaaccgcgg aaatgatgtc ttgtcctgtt tatggtgtcc aagacactcc agatgcgccc
     7501 attaccggaa ccctcgacaa actttgcagg ttctacctgg agaaaatccg agagaagcaa
     7561 cccaaaggtc cataccgcct tgggggtttc tcattcggca cctatgccgc ttttgtcatc
     7621 gcacagctgc tgcaagaaga aggggagaca gttgagctgc tcgtcatgat cgatgggtcg
     7681 ccagcgattt tcaccaactt cgccaagagg atcaatgtga ccaatatgcg agacgaaatc
     7741 atgaccgtta tccgtgatct tgccgcaagc gggacgctgg aaagtggcga ggaacttacc
     7801 gccatgtttg aggaccactt caagcaagtt gcgcagggcg gttctagcgg caaatttgtg
     7861 tcccagttcg gtcgtgcatt cgtcgcacac gtgctcatgg cggtccgcgc ttgtatccca
     7921 gcgatcttac gggagcaagc tggggagatc gataactctg tctggccgac cgcgagaacg
     7981 gtcgttatcc gtgcggaaga tggcattggt cgtcaatccg tgtcggaagg actttcccct
     8041 gcttttgatg tagaattacg cgccccagat gcggagtttt acatcctacc gggaacgcac
     8101 ttcgggattc tccaccccca gtctgggctc cctcagcttc tcgaacaggt ggagaagtgt
     8161 gatcacccaa aagtgatacc accgacgatg acgatgatga ccggaactac gatttctgtg
     8221 ggaaaacaca gaagtcagtc atccatcgac ttaggctgct ctgacacggc gagggcacaa
     8281 gctgcgaaag aagtttccgg gatcatggtg tcatcaatgt ggaggagctg gacagcgaag
     8341 agcaccaagg gtgtgacagc ttcggcattc tcagacagca tcagggtttc cgcctacaat
     8401 gcgtccccca gtaggctcct gctgccttca ttgggggcta atgcaggccc tcaatgcgac
     8461 ggcggacgaa aatggtacca ggccccaaaa atgacctagg gagattgata agacttttct
     8521 agttgcatat cttttatatt taaatcttat ctattagtta attttttgta atttatcctt
     8581 atatatagtc tggttattct aaaatatcat ttcagtatct aaaaattccc ctcttttttc
     8641 agttatatct taacaggcga cagtccaaat gttgatttat cccagtccga ttcatcaggg
     8701 ttgtgaagca ttttgtcaat ggtcgaaatc acatcagtaa tagtgcctct tacttgcctc
     8761 atagaatttc tttctcttaa cgtcaccgtt tggtctttta tagtttcgaa atctatggtg
     8821 ataccaaatg gtgttcccaa ttcatcgtta cgggcgtatt ttttaccaat tgaagtattg
     8881 gaatcgtcaa ttttaaagta tatctctctt ttacgtaaag cctgcgagat cctcttaagt
     8941 atagcgggga agccatcgtt attcgatatt gtcgtaacaa atactttgat cggcgctatg
     9001 cggccgccac cgcggtggag ctccagcttt tgttcccttt agtgagggtt aattgcgcgc
     9061 ttggcgagac tcaaacaaga tatattttgc ccgccttaca gacgaaacta gttggaggtt
     9121 atggagcata ctatcacgtg ggcggccact ggtgagttac tacaccccag gggcaacgtt
     9181 gatgctccta aaaaactctg gctggacgca agccgtaaca cccgtgtcac ttcataatcg
     9241 tttgcaattc agggcttgat ctacacatta ccctgttatc cctattaatt aatacggtta
     9301 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     9361 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     9421 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     9481 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     9541 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     9601 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     9661 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     9721 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     9781 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     9841 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     9901 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     9961 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
    10021 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
    10081 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
    10141 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
    10201 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
    10261 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
    10321 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
    10381 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
    10441 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
    10501 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
    10561 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
    10621 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
    10681 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
    10741 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
    10801 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
    10861 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
    10921 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    10981 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
    11041 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    11101 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgaacgaag catctgtgct
    11161 tcattttgta gaacaaaaat gcaacgcgag agcgctaatt tttcaaacaa agaatctgag
    11221 ctgcattttt acagaacaga aatgcaacgc gaaagcgcta ttttaccaac gaagaatctg
    11281 tgcttcattt ttgtaaaaca aaaatgcaac gcgagagcgc taatttttca aacaaagaat
    11341 ctgagctgca tttttacaga acagaaatgc aacgcgagag cgctatttta ccaacaaaga
    11401 atctatactt cttttttgtt ctacaaaaat gcatcccgag agcgctattt ttctaacaaa
    11461 gcatcttaga ttactttttt tctcctttgt gcgctctata atgcagtctc ttgataactt
    11521 tttgcactgt aggtccgtta aggttagaag aaggctactt tggtgtctat tttctcttcc
    11581 ataaaaaaag cctgactcca cttcccgcgt ttactgatta ctagcgaagc tgcgggtgca
    11641 ttttttcaag ataaaggcat ccccgattat attctatacc gatgtggatt gcgcatactt
    11701 tgtgaacaga aagtgatagc gttgatgatt cttcattggt cagaaaatta tgaacggttt
    11761 cttctatttt gtctctatat actacgtata ggaaatgttt acattttcgt attgttttcg
    11821 attcactcta tgaatagttc ttactacaat ttttttgtct aaagagtaat actagagata
    11881 aacataaaaa atgtagaggt cgagtttaga tgcaagttca aggagcgaaa ggtggatggg
    11941 taggttatat agggatatag cacagagata tatagcaaag agatactttt gagcaatgtt
    12001 tgtggaagcg gtattcgcaa tattttagta gctcgttaca gtccggtgcg tttttggttt
    12061 tttgaaagtg cgtcttcaga gcgcttttgg ttttcaaaag cgctctgaag ttcctatact
    12121 ttctagagaa taggaacttc ggaataggaa cttcaaagcg tttccgaaaa cgagcgcttc
    12181 cgaaaatgca acgcgagctg cgcacataca gctcactgtt cacgtcgcac ctatatctgc
    12241 gtgttgcctg tatatatata tacatgagaa gaacggcata gtgcgtgttt atgcttaaat
    12301 gcgtacttat atgcgtctat ttatgtagga tgaaaggtag tctagtacct cctgtgatat
    12361 tatcccattc catgcggggt atcgtatgct tccttcagca ctacccttta gctgttctat
    12421 atgctgccac tcctcaattg gattagtctc atccttcaat gctatcattt cctttgatat
    12481 tggatcatac taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
    12541 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgag cgcgtttcgg tgatgacggt
    12601 gaaaacctcg gcgcgccatt accctgttat ccctaatata caacgcaaag aagctggaaa
    12661 attggcgagg gaatcctgtt tctgtctatc caagaatggg catgaggtgg caaccgtcgt
    12721 gctagcgtac agggtgcact ttgtaaccat ttgggacacc ggacactcgc tgttttcgaa
    12781 attacccttt aagcgcgggt attgaaccag gcttatgccc aagatcgtag caagcattta
    12841 actgtgggaa tactcaggta tcgtaagatg caagagttcg aatctcttag caaccattat
    12901 ttttttcctc aacataacga gaacacacag gggcgctatc gcacagaatc aaattcgatg
    12961 actggaaatt ttttgttaat ttcagaggtc gcctgacgca tatacctttt tcaactgaaa
    13021 aattgggaga aaaaggaaag gtgagagcgc cggaaccggc ttttcatata gaatagagaa
    13081 gcgttcatga ctaaatgctt gcatcacaat acttgaagtt gacaatatta tttaaggacc
    13141 tattgttttt tccaataggt ggttagcaat cgtcttactt tctaactttt cttacctttt
    13201 acatttcagc aatatatata tatatatttc aaggatatac cattctaatg tctgccccta
    13261 agaagatcgt cgttttgcca ggtgaccacg ttggtcaaga aatcacagcc gaagccatta
    13321 aggttcttaa agctatttct gatgttcgtt ccaatgtcaa gttcgatttc gaaaatcatt
    13381 taattggtgg tgctgctatc gatgctacag gtgttccact tccagatgag gcgctggaag
    13441 cctccaagaa ggctgatgcc gttttgttag gtgctgtggg tggtcctaaa tggggtaccg
    13501 gtagtgttag acctgaacaa ggtttactaa aaatccgtaa agaacttcaa ttgtacgcca
    13561 acttaagacc atgtaacttt gcatccgact ctcttttaga cttatctcca atcaagccac
    13621 aatttgctaa aggtactgac ttcgttgttg tcagagaatt agtgggaggt atttactttg
    13681 gtaagagaaa ggaagacgat ggtgatggtg tcgcttggga tagtgaacaa tacaccgttc
    13741 cagaagtgca aagaatcaca agaatggccg ctttcatggc cctacaacat gagccaccat
    13801 tgcctatttg gtccttggat aaagctaatg ttttggcctc ttcaagatta tggagaaaaa
    13861 ctgtggagga aaccatcaag aacgaattcc ctacattgaa ggttcaacat caattgattg
    13921 attctgccgc catgatccta gttaagaacc caacccacct aaatggtatt ataatcacca
    13981 gcaacatgtt tggtgatatc atctccgatg aagcctccgt tatcccaggt tccttgggtt
    14041 tgttgccatc tgcgtccttg gcctctttgc cagacaagaa caccgcattt ggtttgtacg
    14101 aaccatgcca cggttctgct ccagatttgc caaagaataa ggtcaaccct atcgccacta
    14161 tcttgtctgc tgcaatgatg ttgaaattgt cattgaactt gcctgaagaa ggtaaggcca
    14221 ttgaagatgc agttaaaaag gttttggatg caggtatcag aactggtgat ttaggtggtt
    14281 ccaacagtac caccgaagtc ggtgatgctg tcgccgaaga agttaagaaa atccttgctt
    14341 aaaaagattc tcttttttta tgatatttgt acataaactt tataaatgaa attcataata
    14401 gaaacgacac gaaattacaa aatggaatat gttcataggg tagacgaaac tatatacgca
    14461 atctacatac atttatcaag aaggagaaaa aggaggatgt aaaggaatac aggtaagcaa
    14521 attgatacta atggctcaac gaaatgacgg tatcgataag cttgatatcg aattcctgca
    14581 gcccggggga tccactagtt ctaga
//
