LOCUS       sequence-212376-       13384 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13384)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 13384)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHKU28.1-2.2
FEATURES             Location/Qualifiers
     source          1..13384
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             511..534
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(7815..7835)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7815..7833)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(8172..8760)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8252..8271)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8931..9722,9723..9791))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9554..9573
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9792..9896)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9923..11265
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(10886..10933)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     11306..11324
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11619..12026
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             12027..13121
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(13368..13384)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13368..13384)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 ttaattaata tctaaaaatt gccttatgat ccgtctctcc ggttacagcc tgtgtaactg
       61 attaatcctg cctttctaat caccattcta atgttttaat taagggattt tgtcttcatt
      121 aacggctttc gctcataaaa atgttatgac gttttgcccg caggcgggaa accatccact
      181 tcacgagact gatctcctct gccggaacac cgggcatctc caacttataa gttggagaaa
      241 taagagaatt tcagattgag agaatgaaaa aaaaaaaaaa aaaaaggcag aggagagcat
      301 agaaatgggg ttcacttttt ggtaaagcta tagcatgcct atcacatata aatagagtgc
      361 cagtagcgac ttttttcaca ctcgaaatac tcttactact gctctcttgt tgtttttatc
      421 acttcttgtt tcttcttggt aaatagaata tcaagctaca aaaagcatac aatcaactat
      481 caactattaa ctatatcgta atacacaatg gattataagg atgatgatga taagactagt
      541 atggcgccca ttgccatcac tccggacaac agctccaccg gcagcccgtc aggggagagc
      601 gtcaacggct acttccatgt cagcaccgac aaccgccagg ccaatgggca taccaacggg
      661 cacaccaatg gctacgccag cagacaccta gatggacacc cgaacgggca ttcaaacgga
      721 gccggtaatg gtgttttcaa tggcgtcaat ggcgtcaacg gcgtccacgg gccctcggga
      781 ggtttctcta cttcccataa gccgatgccc atcgccattg tgggaatggc ttgtcgcatg
      841 ccgggcaatg tctctactcc tgccgagttc tgggagctct gcacacgggc tcgctcgggg
      901 tggaccgaga tccccaagga acgattcgac agctcccggt tttaccaccc aaaccccggc
      961 aaggggggcg ccctgaatcc agtcggcggg aacttcctca atgttgatct cgccgccttc
     1021 gacgcccctt tcttcgggct cacagagaag gaagccatat ctatggatcc ccagcagcgc
     1081 cttctcctgg agtgtacgtt cgaggccctc gagaacgcgg gaatccccaa gcacaccatc
     1141 gtcgggaagg acgtcggcgt tttcatcggc ggctcgttct ccgagtacga gtcacacctg
     1201 ttccgggatt ccgatacgat tcccatgcat caggcaaccg gctgcgcatt tgcgatgcag
     1261 tccaaccgta tctcgcattt ctttgacctg agaggcccga gtttcacgtc cgacacagcc
     1321 tgttcgtcca gcatggtcgc tgtgcatctg gcttgccaga gtctgcgggc cggcgagatt
     1381 agctgtgccc tggtcggcgc ctgccatctc aacatgctac cggagttttg gatctccttt
     1441 tcgacttgca ggtacgtccc cgttccagag cccggcacat ccgcaatcag ggacaatgac
     1501 accatcaggg cagtcattgt gggaaccggc ttgaaccagg acggaaagac gcccggcatc
     1561 accatgccaa acggagccgc ccaagaggcc ctgatgagac aggtgtacaa gaacgcggga
     1621 atcgacccac gcgactgcgg cttcgttgaa gctcacggaa cgggtacccg ggtgggggat
     1681 cccatcgagg ccaccgccat ccacaacgtg ctgggccaga acaggacagc acgcgatccc
     1741 ctgtggatag ggtctgtcaa gtccaacatt ggccatctcg aaggcgcttc agggattgct
     1801 ggcatcataa aagctgccct gatgctcgag cgcggtttca tcctccccaa ctacgacttc
     1861 aagcaaccaa accccaagat tccttggaag gagtggaatc tcaaggtccc gattgcccag
     1921 cggccatggc cgcgcggtaa gaaatacatc agcgtcaata actttgggtt tggcgggaca
     1981 aacggtcacg tcgtgctcga ggctccgcca ttccgaggac caaagggttc tgccgccggc
     2041 ggtgacgaca catctggaaa ggcaggacaa ggacggaagc tctacgtctt cacggccaac
     2101 gacaagacgg cgctctcgca ggttatgaag aacatcgtca tttatcttga acagcgccca
     2161 gaaatcttcc aaaaggacct gacaggacat ctagcctaca ccttggggca gcgccgatct
     2221 ctcctgcagt ggagagcggc cattccggcc gtcaattctt tcgagctcat tgaagcgatt
     2281 aacggcgaaa agtacactcc cggaaaggag actgggccgc taaggatcgg cttcattctc
     2341 accgggcagg gcgcgcagtg gcatgccatg ggacgagagc tctatgagca gtatcccacc
     2401 ttcagcagca gcctcgagct ggccgacagg tgtctggctg agaacggtgc tgactggtcc
     2461 ctgatcgagg aactgggaag agacgcaaaa accagcaaga ttaacgaggc ccatatcagt
     2521 caaccgtcgt gtactgccat ccaactggcg ctgattgact tgctgcggtc ctggggcatt
     2581 cgtccaaccg ccgttgctgg tcattcaagc ggcgaaatcg cggccgccta cgccgcggag
     2641 atcatcgact tcgactccgc catgcggatt gcgtaccatc gcggcaggct tattcccatc
     2701 ctcaaacgat ctcatccgga gctcagaggc cgcatgatgg ccgttggcgg cagcaaagag
     2761 cagttcctgc ccattctcga cggcctcaag gagaaggaag cccgcatcgc ctgctacaac
     2821 agcccctcga gcctgaccat ctcgggcgat gagccggcca ttgccgagct cgagaagatc
     2881 tgcgaggaga agcagctctt taacaggcgt ctcgtggtgg acgtggcata ccattcccac
     2941 cacatgaacc tggtggcgaa ggagtaccga gcgtcgctgg ccgacctgcg gccccccgtt
     3001 cccaccgaca tccggttcca ctcatccctt tacgggcgcc tggtcgacgg tacggagctg
     3061 cagccggact actgggtgga gaacctgacc tgcgcagtgc gcttttcgga agcgttgcag
     3121 agcatgctgg aacccgccgg cgagcacaag cacggcgtca acatgctcgt cgagcttggt
     3181 ccgcattccg ggctccaggg gccgatcaag caaatcctga aggagatcgg cggcagcgcc
     3241 cttaagatcc cgtacgcgtc cgctctcgta cggaagcgtg atgcggtaga gacggcgctg
     3301 gaactcgctg cggatctgtt caccaggggc gccagcctca acttcggggc aatcaacttc
     3361 cccaagccga ccaagccgcc cgtgctgctg accgacctgc cgcggtaccc ctggaattac
     3421 tcgtccaggt attggcagga gtctcgcatg acaagagcac acaagcacgc tcctggcggc
     3481 cggagcgaca ttctaggcac cctggccaac tactccaacg acttggagcc cacctggcgc
     3541 aacattgtcc ggctggatga cctcccctgg ctgcggcagc acaagatcca gtccttgact
     3601 gtctttccca tggccgggtt cattgccatg gccctcgagg cagcggctca aagagcatcc
     3661 gcgaggggct gcccgttcga cagtttcgag ctgaaggacg tctcgattac caagcctctc
     3721 gtcattccag acaaggacat cgaggtcacg ctcaccctca gaccacgaaa ggagagcggt
     3781 ccctcctccc ctggcagttg ggacgagttc cgcatctgct cctggtcgag cgaacagggc
     3841 tggaccgagc actgcgttgg cctcattgcg gctctggaag cggatgtcaa cgaggtcgac
     3901 agtgccaggc gcgctcgaga cgcggaggct cgtctcaaga ccatcctgtc tcggactagc
     3961 cagccggaag cggtgcctgt gactccacaa gccatgtatg acagcctctc gaagctgggc
     4021 gtggcctacg gggctctttt ccagggagta accagctgct acgcatccga ctcccacgcg
     4081 gtggggaacc ttgtggtgcc agacgtcgca aaggagatgc ccagcggtta tctaaccgat
     4141 gcggttctcc agccggcgtt cctcgagtct ctcattgaga tgtattggcc cattgtcgga
     4201 gctggtcgac gtcccgtcgg caccatctat ctgccctcgt ctgtcgaccg ggttactgtc
     4261 tctctcggcg tccagcggtt taccaaggac cctggcagcg cggtccgcgc cgtttgcgaa
     4321 ggcgagatcg ccgctggttc gagccccaag ccagccaagg tcaacgtggc cgccgtcacg
     4381 ggcgacgaca agcttgagac gctcatctcc ctggatgggc tcaccatttc ccccatcctc
     4441 gacggcgaga cgcaggcgga ctccgccgca gcgagggaac tctgctataa gttggagtgg
     4501 gatccgatcc tcgaaaccga cgacgagagc gtccaagacc ttccaacgga cgcccaggtc
     4561 gtcatcatcc acgaggactc cagtttccaa agtctcgtgg caatgggcct ggcaaactct
     4621 ctggagcagg ccactgggag gctggctgac tttggcactc tggaaaccgt cgacgctgcc
     4681 ggcaagatct gcgtctttct gagcgagctg caccagccgt tccttgcgac actcacaccc
     4741 gcccagttcg acggcctgca gaggctgttg acgtccgtcg agggcgtact gtgggtcgtg
     4801 cgcggagcgt acgacaactc ggcaaaccca gatgccaaca tggtgaccgg cttgagccga
     4861 accatccgat cagagactgc gctgaagttt gctaccctcg atctggacgc cgagtcaccg
     4921 ctctcggagc ttgagacggc cgacgcgatc atgaaggtct tcaagttcgt ctttcactcg
     4981 ggttcctcca tgtctggaga actggagttc atggagagag gcgggcgttt ctacacgcct
     5041 cgaattgttc acgatcccga gatggacgag tacgtccaca agcagacaaa cccatccgct
     5101 ctcgagccta cgctgtttgg ggaggaccac cgagctttga agatggcact gtcagcgccg
     5161 ggcgcgttgg agacgctcca cttcgttgac gacccggctg ctgagcagcc gctggaggcc
     5221 gacgaggttg agattgaggt taaggccatc ggactgaact tccgggacgg tatggctgcc
     5281 aaaggccaaa tccctctggg cagttgcgga atcgaagcaa gcggagtcgt caccgcggtg
     5341 ggaagcaaag tcacggccta ccgggctggc gatcgtgtcg cggcgcttac cacaggtgcg
     5401 tttgcgacgc gaacgaggac aaaggcggcc ttcgccttca agatctcggc ggacctcaca
     5461 ttcgaggctg ctgccagcct gccgctcgcg tatgcgacag catactacag cttggtagag
     5521 cttgggcggc tcggtgaagg cgagactgtc ctcatccact ccgcggccgg cgctgttgga
     5581 caagcagccg tctgcatcgc acaaatgatc ggtgctgagg tctttgtgac tgttggaagt
     5641 gcggagaaga aagagctcct gttgaaggag ttcggactcc cggacgagcg tgtcttctac
     5701 agccgcaaca gctcctttgc agacgcaatt tgccgagata cgcaggggca tggcgtcgac
     5761 gtcatcctca acaccctcac cggggacggg ctccgcgagt cttggagatg tctgaacaag
     5821 ttcgggcgct tcattgagct tggccgggga gagaatgtct ctaaggcgcg gctggaagtc
     5881 gacagaatcg acagcaacgc cagcttcatc tgccttgaca tgctggccct gatctctgag
     5941 cggccaaaga ccatcaagcg gctacttgcg gacgttgcgc agctcttgaa gtacggcaag
     6001 atcaggcccg cagttcccat cacaagattc cccatctcgg acgtcgaaac ggcgttgaag
     6061 acgcagcaaa ctgggaagtc aatgggcaag ctcgtggtgg ttcccagtgc gactgacgtc
     6121 gtcaaggctg tacaggctgc tcgttctagg aaggcgaagc ccctcctccg ctcggatgcc
     6181 acctatatcc tggtcggcgg cactggtggc ctcggccgca gttttgctag gtggatggcg
     6241 gcgaagggag caaggaacat cgtgctggtt tcgagaagcg gctccgtaac gggcagggta
     6301 aaggagctcg tcgacgaact tggcgcaatt ggcaccaacg tcgttgttcg ccgctgcaac
     6361 gttgtcaaca agagcgaagt ggacgagctt attagctcca tcctcggcga cctgccccct
     6421 atccgcggtg tggtgcatgg caccatggta ctcagggatg tgcttttcga aaagatggcg
     6481 tgggaggatt acacgcaggt catcgagggc aaggtgcaag gcggatggaa cttccaccac
     6541 gcactcgcag actcgccgct cgacttcttc gtggctatct cctcggccgc aggcgccgtc
     6601 ggcaaccgcg ggcaggccgc ctacagcgcg gcgaactgct tcctcaacgc gctggtgcag
     6661 caccgtctcg cccgcggcct gcccgcggcc tcgctcgacc tgaccgccgt ttccgactcg
     6721 ggctacctgg ccgaggacct cgagaaggcg gccgaggtgg cgaggaacct cggcagcgac
     6781 agtatctgcg agagcgaggt gctcgcgctg ctccaggccg ccatcgacgg cacgatggcc
     6841 tcgacctgca acagccacgc catcaccggc atgcgcatca ccgcgaccat gcggccgttc
     6901 tggaccgagg atgccaagtt caaggcgatg cgcatcgcgg ccgaggaggc ggcggccaag
     6961 gacgcttcgc tcaacgccgt ggtgtccttc aatgctgccc tcaagcaggc aaccacgcgc
     7021 gccgaggcgg aggatgtcat ctgccgcggc ctggtcgaca agatctcctc ggtcctgatg
     7081 atggaagccg aggagttgga catcaccagg tcgctgtccc attacccgct ggattcgctg
     7141 gtggcgattg agatccgcaa ctttatcacg cgcgagttcg aggcgaatct gcaggttttg
     7201 gagctgctgt cgagcgggtc gatccagacg cttgcgaagg gggtctgcgc gaagagcaag
     7261 cttgttactt tctcttaggg agattgataa gacttttcta gttgcatatc ttttatattt
     7321 aaatcttatc tattagttaa ttttttgtaa tttatcctta tatatagtct ggttattcta
     7381 aaatatcatt tcagtatcta aaaattcccc tcttttttca gttatatctt aacaggcgac
     7441 agtccaaatg ttgatttatc ccagtccgat tcatcagggt tgtgaagcat tttgtcaatg
     7501 gtcgaaatca catcagtaat agtgcctctt acttgcctca tagaatttct ttctcttaac
     7561 gtcaccgttt ggtcttttat agtttcgaaa tctatggtga taccaaatgg tgttcccaat
     7621 tcatcgttac gggcgtattt tttaccaatt gaagtattgg aatcgtcaat tttaaagtat
     7681 atctctcttt tacgtaaagc ctgcgagatc ctcttaagta tagcggggaa gccatcgtta
     7741 ttcgatattg tcgtaacaaa tactttgatc ggcgctatgc ggccgccacc gcggtggagc
     7801 tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgagact caaacaagat
     7861 atattttgcc cgccttacag acgaaactag ttggaggtta tggagcatac tatcacgtgg
     7921 gcggccactg gtgagttact acaccccagg ggcaacgttg atgctcctaa aaaactctgg
     7981 ctggacgcaa gccgtaacac ccgtgtcact tcataatcgt ttgcaattca gggcttgatc
     8041 tacacattac cctgttatcc ctattaatta atacggttat ccacagaatc aggggataac
     8101 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     8161 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     8221 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     8281 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     8341 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     8401 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     8461 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     8521 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     8581 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     8641 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     8701 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     8761 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     8821 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     8881 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     8941 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     9001 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     9061 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     9121 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     9181 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     9241 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     9301 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     9361 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     9421 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     9481 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     9541 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     9601 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     9661 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     9721 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     9781 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     9841 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     9901 tttccccgaa aagtgccacc tgaacgaagc atctgtgctt cattttgtag aacaaaaatg
     9961 caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc tgcattttta cagaacagaa
    10021 atgcaacgcg aaagcgctat tttaccaacg aagaatctgt gcttcatttt tgtaaaacaa
    10081 aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa
    10141 cagaaatgca acgcgagagc gctattttac caacaaagaa tctatacttc ttttttgttc
    10201 tacaaaaatg catcccgaga gcgctatttt tctaacaaag catcttagat tacttttttt
    10261 ctcctttgtg cgctctataa tgcagtctct tgataacttt ttgcactgta ggtccgttaa
    10321 ggttagaaga aggctacttt ggtgtctatt ttctcttcca taaaaaaagc ctgactccac
    10381 ttcccgcgtt tactgattac tagcgaagct gcgggtgcat tttttcaaga taaaggcatc
    10441 cccgattata ttctataccg atgtggattg cgcatacttt gtgaacagaa agtgatagcg
    10501 ttgatgattc ttcattggtc agaaaattat gaacggtttc ttctattttg tctctatata
    10561 ctacgtatag gaaatgttta cattttcgta ttgttttcga ttcactctat gaatagttct
    10621 tactacaatt tttttgtcta aagagtaata ctagagataa acataaaaaa tgtagaggtc
    10681 gagtttagat gcaagttcaa ggagcgaaag gtggatgggt aggttatata gggatatagc
    10741 acagagatat atagcaaaga gatacttttg agcaatgttt gtggaagcgg tattcgcaat
    10801 attttagtag ctcgttacag tccggtgcgt ttttggtttt ttgaaagtgc gtcttcagag
    10861 cgcttttggt tttcaaaagc gctctgaagt tcctatactt tctagagaat aggaacttcg
    10921 gaataggaac ttcaaagcgt ttccgaaaac gagcgcttcc gaaaatgcaa cgcgagctgc
    10981 gcacatacag ctcactgttc acgtcgcacc tatatctgcg tgttgcctgt atatatatat
    11041 acatgagaag aacggcatag tgcgtgttta tgcttaaatg cgtacttata tgcgtctatt
    11101 tatgtaggat gaaaggtagt ctagtacctc ctgtgatatt atcccattcc atgcggggta
    11161 tcgtatgctt ccttcagcac taccctttag ctgttctata tgctgccact cctcaattgg
    11221 attagtctca tccttcaatg ctatcatttc ctttgatatt ggatcatact aagaaaccat
    11281 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg
    11341 tttcggtgat gacggtgagc gcgtttcggt gatgacggtg aaaacctcgg cgcgccatta
    11401 ccctgttatc cctaatatac aacgcaaaga agctggaaaa ttggcgaggg aatcctgttt
    11461 ctgtctatcc aagaatgggc atgaggtggc aaccgtcgtg ctagcgtaca gggtgcactt
    11521 tgtaaccatt tgggacaccg gacactcgct gttttcgaaa ttacccttta agcgcgggta
    11581 ttgaaccagg cttatgccca agatcgtagc aagcatttaa ctgtgggaat actcaggtat
    11641 cgtaagatgc aagagttcga atctcttagc aaccattatt tttttcctca acataacgag
    11701 aacacacagg ggcgctatcg cacagaatca aattcgatga ctggaaattt tttgttaatt
    11761 tcagaggtcg cctgacgcat ataccttttt caactgaaaa attgggagaa aaaggaaagg
    11821 tgagagcgcc ggaaccggct tttcatatag aatagagaag cgttcatgac taaatgcttg
    11881 catcacaata cttgaagttg acaatattat ttaaggacct attgtttttt ccaataggtg
    11941 gttagcaatc gtcttacttt ctaacttttc ttacctttta catttcagca atatatatat
    12001 atatatttca aggatatacc attctaatgt ctgcccctaa gaagatcgtc gttttgccag
    12061 gtgaccacgt tggtcaagaa atcacagccg aagccattaa ggttcttaaa gctatttctg
    12121 atgttcgttc caatgtcaag ttcgatttcg aaaatcattt aattggtggt gctgctatcg
    12181 atgctacagg tgttccactt ccagatgagg cgctggaagc ctccaagaag gctgatgccg
    12241 ttttgttagg tgctgtgggt ggtcctaaat ggggtaccgg tagtgttaga cctgaacaag
    12301 gtttactaaa aatccgtaaa gaacttcaat tgtacgccaa cttaagacca tgtaactttg
    12361 catccgactc tcttttagac ttatctccaa tcaagccaca atttgctaaa ggtactgact
    12421 tcgttgttgt cagagaatta gtgggaggta tttactttgg taagagaaag gaagacgatg
    12481 gtgatggtgt cgcttgggat agtgaacaat acaccgttcc agaagtgcaa agaatcacaa
    12541 gaatggccgc tttcatggcc ctacaacatg agccaccatt gcctatttgg tccttggata
    12601 aagctaatgt tttggcctct tcaagattat ggagaaaaac tgtggaggaa accatcaaga
    12661 acgaattccc tacattgaag gttcaacatc aattgattga ttctgccgcc atgatcctag
    12721 ttaagaaccc aacccaccta aatggtatta taatcaccag caacatgttt ggtgatatca
    12781 tctccgatga agcctccgtt atcccaggtt ccttgggttt gttgccatct gcgtccttgg
    12841 cctctttgcc agacaagaac accgcatttg gtttgtacga accatgccac ggttctgctc
    12901 cagatttgcc aaagaataag gtcaacccta tcgccactat cttgtctgct gcaatgatgt
    12961 tgaaattgtc attgaacttg cctgaagaag gtaaggccat tgaagatgca gttaaaaagg
    13021 ttttggatgc aggtatcaga actggtgatt taggtggttc caacagtacc accgaagtcg
    13081 gtgatgctgt cgccgaagaa gttaagaaaa tccttgctta aaaagattct ctttttttat
    13141 gatatttgta cataaacttt ataaatgaaa ttcataatag aaacgacacg aaattacaaa
    13201 atggaatatg ttcatagggt agacgaaact atatacgcaa tctacataca tttatcaaga
    13261 aggagaaaaa ggaggatgta aaggaataca ggtaagcaaa ttgatactaa tggctcaacg
    13321 aaatgacggt atcgataagc ttgatatcga attcctgcag cccgggggat ccactagttc
    13381 taga
//
