LOCUS       sequence-212382-       13585 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13585)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 13585)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHSU62.1-2.2
FEATURES             Location/Qualifiers
     source          1..13585
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             511..534
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(6431..6442)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8016..8036)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8016..8034)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(8373..8961)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8453..8472)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9132..9923,9924..9992))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9755..9774
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9993..10097)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      10124..11466
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(11087..11134)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     11507..11525
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11820..12227
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             12228..13322
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(13569..13585)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13569..13585)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 ttaattaata tctaaaaatt gccttatgat ccgtctctcc ggttacagcc tgtgtaactg
       61 attaatcctg cctttctaat caccattcta atgttttaat taagggattt tgtcttcatt
      121 aacggctttc gctcataaaa atgttatgac gttttgcccg caggcgggaa accatccact
      181 tcacgagact gatctcctct gccggaacac cgggcatctc caacttataa gttggagaaa
      241 taagagaatt tcagattgag agaatgaaaa aaaaaaaaaa aaaaaggcag aggagagcat
      301 agaaatgggg ttcacttttt ggtaaagcta tagcatgcct atcacatata aatagagtgc
      361 cagtagcgac ttttttcaca ctcgaaatac tcttactact gctctcttgt tgtttttatc
      421 acttcttgtt tcttcttggt aaatagaata tcaagctaca aaaagcatac aatcaactat
      481 caactattaa ctatatcgta atacacaatg gattataagg atgatgatga taagactagt
      541 atggagaagc cgatgcctat tgccatcatt ggcatggcat gccgcacttc gggcggcgtt
      601 gcttcattag atgaattctg gacgatgctc tctcgctgca gagacggctc tggtccgatt
      661 ccaaaggaca gattctcctc tgctgcatac tatcacccca atccgcaaaa gcgaggcaca
      721 ttcaaccagc ttggaggcta tttcatggac agagatgtgt cctacttcga cgccccgttc
      781 tttaatatca caaggcaaga agcagcttca ctagatccgc tgcaaagaca actcctcgaa
      841 atctcatacg aagctctaga atgcgccggg cttcctaaga acaagatatc gggagagagc
      901 atggccgtct tcgttggtac taagagatca gactaccgat gcaactcaca ggacgtgaag
      961 cgaattgaac tgtttaacgc aacaagcgtt cacccggccg ttcaggcagg ccggctatcc
     1021 tactacttca acctcaacgg gccctgctgc gccgtcgaca cagcctgctc ttctgggctg
     1081 catgctctcc acctaggcgt gaatgccatt cgtaatggag agtgcagttc tgcgcttgtg
     1141 gcggctgcta atttgtatct caacccggat gacctggtat ccatgtcgat gctgggccta
     1201 tttactcctg cgggcaagac atacgccttt gaccaccgcg gaacgtccgg ctttgcctgc
     1261 ggcgaaggcg cggcagccct cgtcatcaag ccgctggatc gggccattaa tgataacgac
     1321 aacatctact ctgtaatcgt caacacagga atcaaccagg atggcaagac ggctggcctc
     1381 acaacgccga gcagtgaaat gcaagagaag cttatgcgtg acgtatacag gcgcgcaaac
     1441 atcagcccta atgacacagc attcgtcgag gctcacggca caggcacaaa agtaggagat
     1501 cccctagaag ttgctgccat ccagcgagtg tttggcaacg ggcgtacaaa gcgcagcccg
     1561 ctatatattg gctcagtaaa gaccaatgtc ggccacctgg aaaatgccag cgggctcatt
     1621 tccgtcatca aggctagtct tatgctgcac cgaggcttca tcttgccaaa caccaatttt
     1681 gagaaggcga atcctgatat cccactggaa gcgtggaatt tgaaggtccc cgtcaatatt
     1741 cgtccctggc ccaggggcag gcgctatatc agcatcaaca actttggttt cggcggctct
     1801 aacgcccatg tcgtccttga taaagcgcct cccgcggtta tgctcagagc tattcctgtc
     1861 gagccgccgg gcagagaagc tatacctaaa ctctttgtca tttccggtca cgacgagggt
     1921 gcagcaagaa gaaatgccag ccaaattgga atctacgtcg agcagcaccc ggaagtcttt
     1981 gagaagcgca tcgctcgcga cgtcgcgtac accctcggcg agcgtcgaac tcaccatgct
     2041 ttccgcgttg ctctcacagc gagcaatttc ggtgaactga cagaagattt gaacgatgag
     2101 gcactactcc cacagcgtgc gtcagatgaa ccacttaagc tagccttcat attcactggc
     2161 caaggtgctc agtggccgca gatgggcaga cagttactca attctcaccc gatcttcggc
     2221 ctgacggtca aggagtgctc cgattttcta gagaacagta ttggcgctga cttctctctt
     2281 atgcaagaac tcttgcggcc aaagggcgag tccatcgtta atgaagctca catttcacag
     2341 ccaatttgca cagcaatcca gctgggtctt gttgagctgc tcaagtcctg gaaaattgag
     2401 ccctccatgg tttgtggcca ttcgtctggt gagatagccg ccgcctatgc tgccggtgcc
     2461 attacgctca gagatgctat ggccgctgct tattaccgcg gtcactttgc cgctcagatg
     2521 aagaaacgca atcctaaagt ccgtggtgcc atgttagctg ttggcgccgg acctgcggaa
     2581 gtaaagagaa tcatcaagtc tctgagttta gccggcgtca ccgtttcttg tgaaaacgct
     2641 cccaattctg taactgcttc tggtgacgaa gccgacattg acagacttgc attagaactt
     2701 gaaggacgca gcttgttcaa ccgtaagctc cgtgtcgatg ttgcatatca ctctgcgcat
     2761 atgaagctcg ttgcgaacga ctacatggca gccattgaga acatgcactt caaggccatg
     2821 gatgatggta tcaagtttta ctcgtctctt cttgggaaaa agcttgatag cactgaagca
     2881 ctgggtgctc agtactgggt taacaacctt atcaagcctg ttctcttttc atctgctctt
     2941 gaggagctct acaatgacgg aaaaccagac atcatcattg aactcggtcc tcactctgca
     3001 ctcgagggcc ctatcaagca agttttaaag ggaattagca agcaggctgc tacaagcgtc
     3061 aggtatcatt ctgctttgct ccgcaaccaa gatgctgcgg ttacagccgt caagcttgcc
     3121 ggtaatcttt ggaccaaagg ccttgatatc gacttcagcc aggtcaatat aacgttggaa
     3181 gggcagttgg agcccggcct ggtttcagac ctaccttcat accagtggtc aaaacatcag
     3241 tgctggacag agtctcgcgc cggcaaatct catcgcctta aggattttgg tcggcacgat
     3301 ctacttggac tcctacagga cgactgcaac gaaactgagc ctgtctggac caatgttctt
     3361 gattctgatg aagtgccatg gctcaaggat cataagatgc agtctcttgt cacattcccc
     3421 tgcgctgggt acctgactat ggcaaccgaa gctgcttctc aaacagcgcg gatgcgtggc
     3481 gtgctgttgg aagaaattgc ctcataccat atgcgaaaca tccaggttag caagcctttc
     3541 atcctcgatg atggcgagtc ttatgacaca cgctttactc tcaagcgtta tgccgaaggt
     3601 ctggcagcgt attctaacga ctgggatgaa tttcaatact cctcttgggc cccttcccga
     3661 ggctgggttg agcactgccg tggcctcgtt ggcatcaaga agcgcagcaa caccaatatt
     3721 gttcgaatga acaaccaaaa agctgctatt gctcgcaggg ccaatatgca gccacagcct
     3781 ggtactgaga tcgattgtga atcgttctac tcagaacttt tgggtaaggg tgctggatac
     3841 accagagact tcactttcaa ggatggtgca aatctgagag tcgatggtga ttgttcagct
     3901 ggcatagcag ttctccagga tgttgctgcc aatatgcctt tcaaccatga gacacaaagc
     3961 actctgccct ctgcattaac agacttgata tttcaaacat gtttcttgat cctcggtgca
     4021 gggcgcggaa atctgaagag cttggtcgtg ccatcggcta tcggaagtgt cgagatttct
     4081 accacatgcc caaatactcc cggcgagacg cttgaaatcg tgacccacca ccaaaacttt
     4141 gcttcaaagg accgattggt atttgacagt gatgcatggc acacaaatgc gagtgagccg
     4201 gtcgttatga tgcgaagcac catcttgacg cccattcatg gtgatgcttt tgaaagtcaa
     4261 gagccacggt ctgcgtgcta tagagtgatc tgggaacctt tggaagccca agttgcagcc
     4321 aaacttgaag acaatgccgc atcagatggg gcaaataacc attccaactg tcacactaat
     4381 gggcacagag atggacacgc caatggttat ataaacggcc atgccaacgg cagcaccaat
     4441 ggacatacaa atggccatat tagtgggtat acaaacggac acaccaatgg acacaccaac
     4501 ggcaacgcca gcaacatcaa cggcaacacc aacggccata caaatggtca taccagtagg
     4561 tataccaatg gacacacaaa cggcaacacc aacggccata caaatggtca tacaaatgga
     4621 cacaccaacg ggcgtaccaa cggttataca aacggccgta ccaatggaca ttcgaatggt
     4681 ggctctaatg ggtataccaa tggtgtgccg acttctggcg ttgacttgag cgccactccc
     4741 atcaccgtca tttgcgatgc tggtccctca aaccctctct gcgctgccct tgcggatctg
     4801 attgagattc agacgtgtgg atgcaggcca accattatat cactagcaaa agcagagata
     4861 tgttcatccg tcagatatgt tgtcttgaat gaggtcgata gcccggtcct cgatgacatg
     4921 aatgccgata catttgctct gatccagaag ctcctgcttg atggcttgtc tatcctctgg
     4981 gtaagctctg gtgcttatct aaatgcagag agcccacata acaacatagc ccagggaatg
     5041 ttccgaacca ttcgttccga gctgggtaaa gtagcggcca ctcttgacct cgataccaaa
     5101 tctaagttgc ggcctgacga tcaggcgcag cttatcatca ctgcaatgaa gacatccatg
     5161 gaaacattca aagatgaatc catcgattat gaatttgcgg agaaagatgg gaaacttctc
     5221 gttccccgtg tcgtagaaga aaatgagatg aatcttgacc tcttccgcca aacccagtct
     5281 cctccccctt atctccaaaa cttcaacgag cccaaccgcc gcctcaagat cgcggttggc
     5341 acatacggtg cgctcgactc cctattctgg gacgacgagg agacatacgg gcttgggcca
     5401 aacgagattg aagttaaagt tgctgctact ggcatgaact tcaaagacgt tgtcattgcc
     5461 atgggacaag tgcctagccc gtacctcgga atcgagtgtt ccggtaccgt ctcacgcatt
     5521 ggatctggtg tcacctcgct caagattgga aatcgagttt gtgctatgac acacggtgct
     5581 tatagtactt ttgctcgctg tccagctact agcgcagctg tcatcccaga ggatattgca
     5641 ttctccacag ccgcttctat ccccgttatc ttctgcacag cctactatgg cctgatagag
     5701 cttgctcacc ttgaacgtgg ggagaaaata ttaattcacg cagcgtctgg cggcgtggga
     5761 caggccgcca ttcagctctc ccagatggta ggcgctgaga tttttgccac taccagcagt
     5821 gttgagaaga aaaaccatct tattgatacc tatggtattc cggaggacca tatcttttat
     5881 agtagagata ccagttttgg gcctgccgtc agagaatcta ccgacgggaa aggtgtagat
     5941 gttgtaatca actcccttgc tggtgatctg ctgcgagaaa cctgggcctg cattgcccca
     6001 tttggccgct ttattgaaat tggaaaacgt gatatcacct ccaacactcg actggaaatg
     6061 gacaaatttg aatacaattg ctctttccac tcggttgatt tgtccaaggt tgctgactgg
     6121 cgaccaaaga taatgagtcg agttttcaag gaagtcatgg ctctcatctc caaaaggatt
     6181 atccagccca ttagccccat cactgagatt ggcatctcta aggtcgaagc tgcgttgcgc
     6241 aaacttcaga gcggcaagac taagggcaag gtcattgtca accatctcgt caatgagcag
     6301 gtcaatgctg cccacagaca gcctaccgga gacttagtga atggaaatgc cacttatatc
     6361 atcattggcg gtacaggtgg ccttggtcgt tccatggcaa agaaaatggc tcagcgcggt
     6421 ggaaagcacg tcgtccttct atcccgcagc ggcaagatga ctcctgagct agatcatctc
     6481 agcaaagaat gcgagatata tggagccaag atctatgtaa aatgctgtga cgcggccaac
     6541 caggcaaaag tcaacgaact tatctcagag ctgcaatcgt cactgcctcc gatccgtggt
     6601 gtcatccatg cagccatggt gctgcgggat accctctttg aaaagatgtc attcgaggac
     6661 tgggatgagg ttgtgcgctg taaggttgcg ggaggctgga acttccacat tgccttagca
     6721 aaggagcccc tcgacttctt catcgtccta tcctcggttg ctggcattgt tggcaaccgc
     6781 ggccaggcag cctacgccgc tgccaacacc ttcttagatg ccttgacagt ccaccgccgt
     6841 aggttgggtc ttaaagctac atcgctcaac ctaactgcag tcgaggatat cggttatctg
     6901 gcggagaatt ccggcaagca aggcgaagtt cttcgcaatt tatctggtaa cggaattggc
     6961 ggggccgaag tcctagcact catcgagagt tctataaacg gcaaggttgg caccgcctgc
     7021 gacgaccagt gtatcaccgg acttgaattc ggggactcga tgccctactt tgccgcggat
     7081 ggcaagttca agtatcttcg tcaggctgct cttgcgctga gcgctgctgc gggtgatgac
     7141 ggtggcgcga atattcctat tggcaagaga ctagctaggg cgatgacagt gggcgaagcc
     7201 gtcgagcttg ttaccgatgg cttgcgtgac aaactgggcg ccattctgat gcttgacccc
     7261 gatgtcattc tagctaaaca ggcgactgtc accatggctg cccttggctt ggactcactc
     7321 aacgctatcg agctgcgtaa ttggatcgca agggacctgc agacacactt gcagatcctg
     7381 gagctgctgc gagctggaac gttggctgat cttgcaagtc agattctgaa gaagactcgc
     7441 atccaaggtg cttggacaat gcaggaggga gccagataag gagattgata agacttttct
     7501 agttgcatat cttttatatt taaatcttat ctattagtta attttttgta atttatcctt
     7561 atatatagtc tggttattct aaaatatcat ttcagtatct aaaaattccc ctcttttttc
     7621 agttatatct taacaggcga cagtccaaat gttgatttat cccagtccga ttcatcaggg
     7681 ttgtgaagca ttttgtcaat ggtcgaaatc acatcagtaa tagtgcctct tacttgcctc
     7741 atagaatttc tttctcttaa cgtcaccgtt tggtctttta tagtttcgaa atctatggtg
     7801 ataccaaatg gtgttcccaa ttcatcgtta cgggcgtatt ttttaccaat tgaagtattg
     7861 gaatcgtcaa ttttaaagta tatctctctt ttacgtaaag cctgcgagat cctcttaagt
     7921 atagcgggga agccatcgtt attcgatatt gtcgtaacaa atactttgat cggcgctatg
     7981 cggccgccac cgcggtggag ctccagcttt tgttcccttt agtgagggtt aattgcgcgc
     8041 ttggcgagac tcaaacaaga tatattttgc ccgccttaca gacgaaacta gttggaggtt
     8101 atggagcata ctatcacgtg ggcggccact ggtgagttac tacaccccag gggcaacgtt
     8161 gatgctccta aaaaactctg gctggacgca agccgtaaca cccgtgtcac ttcataatcg
     8221 tttgcaattc agggcttgat ctacacatta ccctgttatc cctattaatt aatacggtta
     8281 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     8341 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     8401 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     8461 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     8521 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     8581 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     8641 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     8701 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     8761 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     8821 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     8881 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     8941 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     9001 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     9061 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     9121 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     9181 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     9241 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     9301 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     9361 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     9421 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     9481 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     9541 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     9601 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     9661 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     9721 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     9781 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     9841 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     9901 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     9961 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
    10021 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    10081 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgaacgaag catctgtgct
    10141 tcattttgta gaacaaaaat gcaacgcgag agcgctaatt tttcaaacaa agaatctgag
    10201 ctgcattttt acagaacaga aatgcaacgc gaaagcgcta ttttaccaac gaagaatctg
    10261 tgcttcattt ttgtaaaaca aaaatgcaac gcgagagcgc taatttttca aacaaagaat
    10321 ctgagctgca tttttacaga acagaaatgc aacgcgagag cgctatttta ccaacaaaga
    10381 atctatactt cttttttgtt ctacaaaaat gcatcccgag agcgctattt ttctaacaaa
    10441 gcatcttaga ttactttttt tctcctttgt gcgctctata atgcagtctc ttgataactt
    10501 tttgcactgt aggtccgtta aggttagaag aaggctactt tggtgtctat tttctcttcc
    10561 ataaaaaaag cctgactcca cttcccgcgt ttactgatta ctagcgaagc tgcgggtgca
    10621 ttttttcaag ataaaggcat ccccgattat attctatacc gatgtggatt gcgcatactt
    10681 tgtgaacaga aagtgatagc gttgatgatt cttcattggt cagaaaatta tgaacggttt
    10741 cttctatttt gtctctatat actacgtata ggaaatgttt acattttcgt attgttttcg
    10801 attcactcta tgaatagttc ttactacaat ttttttgtct aaagagtaat actagagata
    10861 aacataaaaa atgtagaggt cgagtttaga tgcaagttca aggagcgaaa ggtggatggg
    10921 taggttatat agggatatag cacagagata tatagcaaag agatactttt gagcaatgtt
    10981 tgtggaagcg gtattcgcaa tattttagta gctcgttaca gtccggtgcg tttttggttt
    11041 tttgaaagtg cgtcttcaga gcgcttttgg ttttcaaaag cgctctgaag ttcctatact
    11101 ttctagagaa taggaacttc ggaataggaa cttcaaagcg tttccgaaaa cgagcgcttc
    11161 cgaaaatgca acgcgagctg cgcacataca gctcactgtt cacgtcgcac ctatatctgc
    11221 gtgttgcctg tatatatata tacatgagaa gaacggcata gtgcgtgttt atgcttaaat
    11281 gcgtacttat atgcgtctat ttatgtagga tgaaaggtag tctagtacct cctgtgatat
    11341 tatcccattc catgcggggt atcgtatgct tccttcagca ctacccttta gctgttctat
    11401 atgctgccac tcctcaattg gattagtctc atccttcaat gctatcattt cctttgatat
    11461 tggatcatac taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
    11521 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgag cgcgtttcgg tgatgacggt
    11581 gaaaacctcg gcgcgccatt accctgttat ccctaatata caacgcaaag aagctggaaa
    11641 attggcgagg gaatcctgtt tctgtctatc caagaatggg catgaggtgg caaccgtcgt
    11701 gctagcgtac agggtgcact ttgtaaccat ttgggacacc ggacactcgc tgttttcgaa
    11761 attacccttt aagcgcgggt attgaaccag gcttatgccc aagatcgtag caagcattta
    11821 actgtgggaa tactcaggta tcgtaagatg caagagttcg aatctcttag caaccattat
    11881 ttttttcctc aacataacga gaacacacag gggcgctatc gcacagaatc aaattcgatg
    11941 actggaaatt ttttgttaat ttcagaggtc gcctgacgca tatacctttt tcaactgaaa
    12001 aattgggaga aaaaggaaag gtgagagcgc cggaaccggc ttttcatata gaatagagaa
    12061 gcgttcatga ctaaatgctt gcatcacaat acttgaagtt gacaatatta tttaaggacc
    12121 tattgttttt tccaataggt ggttagcaat cgtcttactt tctaactttt cttacctttt
    12181 acatttcagc aatatatata tatatatttc aaggatatac cattctaatg tctgccccta
    12241 agaagatcgt cgttttgcca ggtgaccacg ttggtcaaga aatcacagcc gaagccatta
    12301 aggttcttaa agctatttct gatgttcgtt ccaatgtcaa gttcgatttc gaaaatcatt
    12361 taattggtgg tgctgctatc gatgctacag gtgttccact tccagatgag gcgctggaag
    12421 cctccaagaa ggctgatgcc gttttgttag gtgctgtggg tggtcctaaa tggggtaccg
    12481 gtagtgttag acctgaacaa ggtttactaa aaatccgtaa agaacttcaa ttgtacgcca
    12541 acttaagacc atgtaacttt gcatccgact ctcttttaga cttatctcca atcaagccac
    12601 aatttgctaa aggtactgac ttcgttgttg tcagagaatt agtgggaggt atttactttg
    12661 gtaagagaaa ggaagacgat ggtgatggtg tcgcttggga tagtgaacaa tacaccgttc
    12721 cagaagtgca aagaatcaca agaatggccg ctttcatggc cctacaacat gagccaccat
    12781 tgcctatttg gtccttggat aaagctaatg ttttggcctc ttcaagatta tggagaaaaa
    12841 ctgtggagga aaccatcaag aacgaattcc ctacattgaa ggttcaacat caattgattg
    12901 attctgccgc catgatccta gttaagaacc caacccacct aaatggtatt ataatcacca
    12961 gcaacatgtt tggtgatatc atctccgatg aagcctccgt tatcccaggt tccttgggtt
    13021 tgttgccatc tgcgtccttg gcctctttgc cagacaagaa caccgcattt ggtttgtacg
    13081 aaccatgcca cggttctgct ccagatttgc caaagaataa ggtcaaccct atcgccacta
    13141 tcttgtctgc tgcaatgatg ttgaaattgt cattgaactt gcctgaagaa ggtaaggcca
    13201 ttgaagatgc agttaaaaag gttttggatg caggtatcag aactggtgat ttaggtggtt
    13261 ccaacagtac caccgaagtc ggtgatgctg tcgccgaaga agttaagaaa atccttgctt
    13321 aaaaagattc tcttttttta tgatatttgt acataaactt tataaatgaa attcataata
    13381 gaaacgacac gaaattacaa aatggaatat gttcataggg tagacgaaac tatatacgca
    13441 atctacatac atttatcaag aaggagaaaa aggaggatgt aaaggaatac aggtaagcaa
    13501 attgatacta atggctcaac gaaatgacgg tatcgataag cttgatatcg aattcctgca
    13561 gcccggggga tccactagtt ctaga
//
