LOCUS       sequence-212383-       12088 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12088)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 12088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHSU62.2-2.2
FEATURES             Location/Qualifiers
     source          1..12088
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1271..1570
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
     promoter        4479..4699
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             4700..5503
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     primer_bind     complement(8221..8241)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8221..8239)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(8578..9166)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8658..8677)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9337..10128,10129..10197))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9960..9979
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10198..10302)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      10329..11671
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(11292..11339)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     11712..11730
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(12072..12088)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12072..12088)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 ttaattaata tctaaaaatt gccttatgat ccgtctctcc ggttacagcc tgtgtaactg
       61 attaatcctg cctttctaat caccattcta atgttttaat taagggattt tgtcttcatt
      121 aacggctttc gctcataaaa atgttatgac gttttgcccg caggcgggaa accatccact
      181 tcacgagact gatctcctct gccggaacac cgggcatctc caacttataa gttggagaaa
      241 taagagaatt tcagattgag agaatgaaaa aaaaaaaaaa aaaaaaggca gaggagagca
      301 tagaaatggg gttcactttt tggtaaagct atagcatgcc tatcacatat aaatagagtg
      361 ccagtagcga cttttttcac actcgaaata ctcttactac tgctctcttg ttgtttttat
      421 cacttcttgt ttcttcttgg taaatagaat atcaagctac aaaaagcata caatcaacta
      481 tcaactatta actatatcgt aatacacaat gaagatcctc tgtatccatg gctcgtatgg
      541 cagcgcatct gcctttcgca cccagctcga accctttctc gcccagatag agaagcctgg
      601 ggaggtggat ttcaaattca tcgacggcct ctatcctgcc gtgcctccag atggctttca
      661 ggactacttt ggcccgccac ctcacttcct caacgttcaa tacgacggta ttgctggctt
      721 agagaacatg caattgaacc tgcgtacact caaaccagga gaaagcttcg aagacacaat
      781 aaagaggctt atcaaaaatg atgccacagt cgtgtctaaa gaagaatata tgaagaccct
      841 cgactacatt gagcagtgcc tggtcgagga tccagaaatt cagggaattc ttggttactc
      901 cgaaggtgct acgactgctg caacatattt gttacagcag gcacaccggc accgagacga
      961 aggcatccca cacagggtca agtttggcat cttcttcggg ggctggccgc ctgcaaagtt
     1021 cgaaggtgac cgccttgcgc ccgtcctacc cgaggacgat gatatgattg atatcccaac
     1081 atgccacatt gtcggctgta gcgaccccta tctttacggc gccatgaccc tgtttcaggt
     1141 ctgcgatgaa gagacagcca ccctattcga tcatggcaag ggtcatttgc tgcctcgaga
     1201 tcccatgacc attgccgagt tatcagaatg catcaacaca actctcaagc aggcaggttt
     1261 ccttaactag gcggatctct tatgtcttta cgatttatag ttttcattat caagtatgcc
     1321 tatattagta tatagcatct ttagatgaca gtgttcgaag tttcacgaat aaaagataat
     1381 attctacttt ttgctcccac cgcgtttgct agcacgagtg aacaccatcc ctcgcctgtg
     1441 agttgtaccc attcctctaa actgtagaca tggtagcttc agcagtgttc gttatgtacg
     1501 gcatcctcca acaaacagtc ggttatagtt tgtcctgctc ctctgaatcg tctccctcga
     1561 tatttctcat tttccttcgc atgccagcat tgaaatgatc gaagttcaat gatgaaacgg
     1621 taattcttct gtcatttact catctcatct catcaagtta tataattcta tacggatgta
     1681 atttttcact tttcgtcttg acgtccaccc tataatttca attattgaac cctcacgatc
     1741 cagttctcca gtgacacagc ctttatctgg tcaaaccttt ctttctaatc acctatgctg
     1801 atgcttaatt aagggatttt tgtctccatc aacggcatgc gcccaaaaat gacgtttttt
     1861 ttaacccata gacacgaaac tacccatttt ccaccggcct gacctaccac cggaacaacg
     1921 gccatctcca acttgcaagt tggggaaatt aagagcatcg caggtttaat ggaagaaaaa
     1981 aaaaaggtac agcacagcgc aaatggagtt agttccctta tgtcacacac tcacacacag
     2041 tcggtcagat caagcatact gggtgcgtat aaatagagtg gccattgcca ccctgtttat
     2101 ctcaaaatct gtcttgttag tggtcttctc cctttttcag gttacaattc tcttgtttct
     2161 acttagtata taagtatatc aagctatatt aagcatacta tcaactgtca actctatcct
     2221 caaaatacaa tacaaaatgg acgacttgct ggctctcgtc gagggtggcc ctgcgctcaa
     2281 gccgacgatc aactcactct ggacagccct gcaccagcgt gccagcatac agccagacag
     2341 actggccctg ctgagccccc ggcagcctgg aaacttgctc gaaggactcg tccggcctag
     2401 aggaaggaga gatgagagtg gcagccgcga tgccctcatg cgtagcctca agcgagcagc
     2461 cacaccggcg tacatgctca gctacctgct atcgtgggtt ctgccggcgc ctgatacaga
     2521 gacagactac ctcagatggt cctttgcaga gctccaacgt gcaacaattc gtctagcttc
     2581 ttttctgcgt cgtcgtggtg tgaagccagg ggcgacaggc gccttcttca ccccggtctg
     2641 cgcagaatgg gcgttgatgc tgtctgtgtc tgcgtacagt cgctacacgg gtgtgacgct
     2701 cgaccggaag acgctcgcgc ctggtaatga gcacgacctg cgagtacata tgaacacact
     2761 aagcccgtct ttcattatcg ttactacggt agaggaagcg ctgcatcttg ctggacagac
     2821 agatgtagca ctcgggatct gcttggagaa cttcgcgacg cctgctccgg agggctggct
     2881 gagcatggca gatattgcag catcctcata tgaagaggaa gaggaagaag aagtagaaga
     2941 agcagaagaa gtggaagaaa agagagaaag tgacaggaca gccttcgttg tgtttactag
     3001 tggctcgtcc ggcataccca agggctgccc agtgtccgcc agccaaattc tatctcttct
     3061 cggagctgtg tccaagcaga tgcctctcct accaactcct atgactcttg tctcgggcac
     3121 atgcttccgg tcgctctgca cagccatcac actgatgtcc tgggcctcag gcaacgccgc
     3181 cgtcatggac gccagcaacg aggcgacagc tgagacgaag ctcgctgggc tgcaaacatg
     3241 ccgacctgcc tttgcctccg taaccgtcac ggcgctctcc tctattgccc gtagcccaaa
     3301 ctacaccgcc gacgccatca ggtcggtccg cttcgtccat ctcatcggct caactatgac
     3361 cgcgtcggtc ctccgaaggg ccaagaagac gttccctcac gcaacgatcc tgcccagctt
     3421 tggcatgtca gaggccgcaa agacagtgac ctgggaagct gccggaggtg tgccatccgt
     3481 tgacaagata cctacatggc acggcatcgc cgcctcaggc aaggtggctc ccggcgaaag
     3541 gctcaaggtc gttgatatag acggtaacgt tgcacgccgt aatcatgttg gtgcgttaca
     3601 cctgagcggc gactcagtta tcggtggtta cttgggtggt gccaattctg ctgcctttta
     3661 cgtaggagac gacggccacc actggttcgt ctctggggac gatgccgtga ttgatgacca
     3721 aggccttctc tatgtcctcg gtcgaagtga gtatactctc aggcgcgaca gcaagctcat
     3781 cctcccctgt atcgttgaaa attttctcga gacgcaattc gaagggtcct cggctgccgt
     3841 agtgaatatc accaccagca acggtgatag gggtttattt gctgtcttag agcacgctgt
     3901 ccctgcccct gcatccgctg ctgttcaaga acttgtcgct caaaagcttg gcttggaata
     3961 ccgtgtcgaa ggtgctgtga gcctcaatga gctaggattc gagaagtggc cgctgacctc
     4021 gggagaaaaa gtcgcctacc gtgatttacg gctcgccatt gaggaatttc tcacttccaa
     4081 gacggcaaat ttatgaacaa atcgctctta aatatatacc taaagaacat taaagctata
     4141 ttataagcaa agatacgtaa attttgctta tattattata cacatatcat atttctatat
     4201 ttttaagatt tggttatata atgtacgtaa tgcaaaggaa ataaatttta tacattattg
     4261 aacagcgtcc aagtaactac attatgtgca ctaatagttt agcgtcgtga agactttatt
     4321 gtgtcgcgaa aagtaaaaat tttaaaaatt agagcacctt gaacttgcga aaaaggttct
     4381 catcaactgt ttaaaaggag gatatcaggt cctatttctg acaaacaata tacaaattta
     4441 gtttcaaaga tgaatcagtg cgcgaaggac ataactcatt caattcaatt catcattttt
     4501 tttttattct tttttttgat ttcggtttcc ttgaaatttt tttgattcgg taatctccga
     4561 acagaaggaa gaacgaagga aggagcacag acttagattg gtatatatac gcatatgtag
     4621 tgttgaagaa acatgaaatt gcccagtatt cttaacccaa ctgcacagaa caaaaacctg
     4681 caggaaacga agataaatca tgtcgaaagc tacatataag gaacgtgctg ctactcatcc
     4741 tagtcctgtt gctgccaagc tatttaatat catgcacgaa aagcaaacaa acttgtgtgc
     4801 ttcattggat gttcgtacca ccaaggaatt actggagtta gttgaagcat taggtcccaa
     4861 aatttgttta ctaaaaacac atgtggatat cttgactgat ttttccatgg agggcacagt
     4921 taagccgcta aaggcattat ccgccaagta caatttttta ctcttcgaag acagaaaatt
     4981 tgctgacatt ggtaatacag tcaaattgca gtactctgcg ggtgtataca gaatagcaga
     5041 atgggcagac attacgaatg cacacggtgt ggtgggccca ggtattgtta gcggtttgaa
     5101 gcaggcggca gaagaagtaa caaaggaacc tagaggcctt ttgatgttag cagaattgtc
     5161 atgcaagggc tccctatcta ctggagaata tactaagggt actgttgaca ttgcgaagag
     5221 cgacaaagat tttgttatcg gctttattgc tcaaagagac atgggtggaa gagatgaagg
     5281 ttacgattgg ttgattatga cacccggtgt gggtttagat gacaagggag acgcattggg
     5341 tcaacagtat agaaccgtgg atgatgtggt ctctacagga tctgacatta ttattgttgg
     5401 aagaggacta tttgcaaagg gaagggatgc taaggtagag ggtgaacgtt acagaaaagc
     5461 aggctgggaa gcatatttga gaagatgcgg ccagcaaaac taaaaaactg tattataagt
     5521 aaatgcatgt atactaaact cacaaattag agcttcaatt taattatatc agttattacc
     5581 ctatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggataggaa
     5641 aaaaccgagc ttcctttcat ccggcgcggc tgtgttctac atatcactga agctccgggt
     5701 attttaagtt atacaaggga aagatgccgg ctagactagc aagttttagg ctgcttaaca
     5761 ttatggatag gcggataaag ggcccaaaca ggattgtaaa gcttagacgc ttctggttgg
     5821 acaatggtac gtttgtgtat taagtaaggc ttggctgggg atagcaacat tgggcagagt
     5881 atagaagacc acaaaaaaaa ggtatataag ggcagagaag tctttgtaat gtgtgtaact
     5941 tctcttccat gtgtaatcag tatttctact tacttcttaa atatacagaa gtaagacaga
     6001 taaccaacag cctttcccag atatacatat atatctttat ttcagcttaa acaataatta
     6061 tatttgttta actcaaaaat aaaaaaaaaa aaccaaactc acgcaactaa ttattccata
     6121 ataaaataac aacatgccgc gcttcaaccc cctaagcatc tcctgtctgg ctactctgct
     6181 gaccacctac cttggtacag catctgtatc tgcacagtca ccaccccagg aaaccctctg
     6241 gccggtgcag acgttccaga gtacggatat ccagacgccg tttttcaatg tgacgaagaa
     6301 aggccagacg gaaccgggct atatcttcat aagcccgaga gataaggccc gaaacattgg
     6361 acatccagct atcctcgagg acaatggcca ggttgtctgg aagggaccgg aagacgcgag
     6421 cacatatggc ttcaagcctc agcttcttga cgggaagcca gtgctggtta gctggttcgg
     6481 gttcgtgaac gagactggat tcggactggg atctatgagc atcttcgaca gctcgtatga
     6541 gaagatccac gaggtgattc tgcccggtgg tgataacgaa tactacaaga cgatttatga
     6601 gcccatgacg ttcccatcat acatggacaa ccacgaaggc cagatcacgg accaaggaac
     6661 catcgttgtg actgttgtca atgtcactga ggcagatctc cgtgctgttg gcgggcctga
     6721 gaagggctgg gttgtcgacg gaggcttttt ggaaatggac atcaagacca acgaagtcct
     6781 cttccgctgg aatgccgctg agcacattga tgagatccct gtcactttct cccttaagcc
     6841 aattgaggga actggaactt cgcccaaaaa cccatggaac tacgtccatg tcaactccgt
     6901 cttcaaatcc ggagactctt atatcgtctc ttctcggtac tcctgcaaca tcttcctcat
     6961 ctccaaggaa ggcgagattg tctggcgcct caacggcatt gatggaggtg acttcgagct
     7021 aggccccggc gccaacttct gttaccagca tgaagtcagg gtcgaatccc acaccgtgga
     7081 caagatcacg ctgactacac acaacaacga caacgccgac ttcacgcctg ccaatgaaac
     7141 caaaccgaca accggtctgg tcctcgatct cgatctcaac gccaagaaag tcactttgaa
     7201 ccgcatggta tggaactccg agcggcctgt cgtctctcgg ggccagggga gctaccaggt
     7261 gcttggcaac gaccacgtcc tgatgggcca gggcgccatc cccatcgtag aggagtacga
     7321 tgccaacggc gccgtcgtaa tggacgcccg tttcggcaac gacggcgtca ccaactgcta
     7381 ccgcgcctac cgcagcgcct gggttggcac acccaagacg aaaccgagcg taaaggcctg
     7441 cggtgccgat ccgaacggcg cggtggtgtt ttatgtgagc tggaacggcg cgacggacgt
     7501 agagtcgtgg aagattttct ctgtctcgga ctctggcgag cacagtgaga tagctaatgt
     7561 gccgaaaaat ggctttgaga cgaggatgga gctgcagaag gctggcagca agttcatcgt
     7621 ccagtcggtt ggaggaccta acgacggggt gcagtctgat gttgtcatgg gcgaaaactg
     7681 ctagggagat tgataagact tttctagttg catatctttt atatttaaat cttatctatt
     7741 agttaatttt ttgtaattta tccttatata tagtctggtt attctaaaat atcatttcag
     7801 tatctaaaaa ttcccctctt ttttcagtta tatcttaaca ggcgacagtc caaatgttga
     7861 tttatcccag tccgattcat cagggttgtg aagcattttg tcaatggtcg aaatcacatc
     7921 agtaatagtg cctcttactt gcctcataga atttctttct cttaacgtca ccgtttggtc
     7981 ttttatagtt tcgaaatcta tggtgatacc aaatggtgtt cccaattcat cgttacgggc
     8041 gtatttttta ccaattgaag tattggaatc gtcaatttta aagtatatct ctcttttacg
     8101 taaagcctgc gagatcctct taagtatagc ggggaagcca tcgttattcg atattgtcgt
     8161 aacaaatact ttgatcggcg ctatgcggcc gccaccgcgg tggagctcca gcttttgttc
     8221 cctttagtga gggttaattg cgcgcttggc gagactcaaa caagatatat tttgcccgcc
     8281 ttacagacga aactagttgg aggttatgga gcatactatc acgtgggcgg ccactggtga
     8341 gttactacac cccaggggca acgttgatgc tcctaaaaaa ctctggctgg acgcaagccg
     8401 taacacccgt gtcacttcat aatcgtttgc aattcagggc ttgatctaca cattaccctg
     8461 ttatccctat taattaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     8521 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     8581 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     8641 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     8701 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     8761 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     8821 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     8881 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     8941 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     9001 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     9061 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     9121 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     9181 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     9241 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     9301 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     9361 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     9421 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     9481 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     9541 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     9601 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     9661 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     9721 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     9781 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     9841 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     9901 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     9961 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
    10021 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
    10081 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    10141 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    10201 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    10261 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    10321 gccacctgaa cgaagcatct gtgcttcatt ttgtagaaca aaaatgcaac gcgagagcgc
    10381 taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc aacgcgaaag
    10441 cgctatttta ccaacgaaga atctgtgctt catttttgta aaacaaaaat gcaacgcgag
    10501 agcgctaatt tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc
    10561 gagagcgcta ttttaccaac aaagaatcta tacttctttt ttgttctaca aaaatgcatc
    10621 ccgagagcgc tatttttcta acaaagcatc ttagattact ttttttctcc tttgtgcgct
    10681 ctataatgca gtctcttgat aactttttgc actgtaggtc cgttaaggtt agaagaaggc
    10741 tactttggtg tctattttct cttccataaa aaaagcctga ctccacttcc cgcgtttact
    10801 gattactagc gaagctgcgg gtgcattttt tcaagataaa ggcatccccg attatattct
    10861 ataccgatgt ggattgcgca tactttgtga acagaaagtg atagcgttga tgattcttca
    10921 ttggtcagaa aattatgaac ggtttcttct attttgtctc tatatactac gtataggaaa
    10981 tgtttacatt ttcgtattgt tttcgattca ctctatgaat agttcttact acaatttttt
    11041 tgtctaaaga gtaatactag agataaacat aaaaaatgta gaggtcgagt ttagatgcaa
    11101 gttcaaggag cgaaaggtgg atgggtaggt tatataggga tatagcacag agatatatag
    11161 caaagagata cttttgagca atgtttgtgg aagcggtatt cgcaatattt tagtagctcg
    11221 ttacagtccg gtgcgttttt ggttttttga aagtgcgtct tcagagcgct tttggttttc
    11281 aaaagcgctc tgaagttcct atactttcta gagaatagga acttcggaat aggaacttca
    11341 aagcgtttcc gaaaacgagc gcttccgaaa atgcaacgcg agctgcgcac atacagctca
    11401 ctgttcacgt cgcacctata tctgcgtgtt gcctgtatat atatatacat gagaagaacg
    11461 gcatagtgcg tgtttatgct taaatgcgta cttatatgcg tctatttatg taggatgaaa
    11521 ggtagtctag tacctcctgt gatattatcc cattccatgc ggggtatcgt atgcttcctt
    11581 cagcactacc ctttagctgt tctatatgct gccactcctc aattggatta gtctcatcct
    11641 tcaatgctat catttccttt gatattggat catactaaga aaccattatt atcatgacat
    11701 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg
    11761 gtgagcgcgt ttcggtgatg acggtgaaaa cctcggcgcg ccattaccct gttatcccta
    11821 atatacaacg caaagaagct ggaaaattgg cgagggaatc ctgtttctgt ctatccaaga
    11881 atgggcatga ggtggcaacc gtcgtgctag cgtacagggt gcactttgta accatttggg
    11941 acaccggaca ctcgctgttt tcgaaattac cctttaagcg cgggtattga accaggctta
    12001 tgcccaagat cgtagcaagc atttaaatga cggtatcgat aagcttgata tcgaattcct
    12061 gcagcccggg ggatccacta gttctaga
//
