LOCUS       sequence-212411-       13441 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13441)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 13441)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHKU27.1-2.2
FEATURES             Location/Qualifiers
     source          1..13441
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1..17)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1..17)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             528..551
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      2292..2301
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(7889..7909)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7889..7907)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(8246..8834)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8326..8345)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9005..9796,9797..9865))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9628..9647
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9866..9970)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9997..11339
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(10960..11007)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     11380..11398
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11693..12100
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             12101..13195
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
ORIGIN
        1 gatccactag ttctagatta attaatatct aaaaattgcc ttatgatccg tctctccggt
       61 tacagcctgt gtaactgatt aatcctgcct ttctaatcac cattctaatg ttttaattaa
      121 gggattttgt cttcattaac ggctttcgct cataaaaatg ttatgacgtt ttgcccgcag
      181 gcgggaaacc atccacttca cgagactgat ctcctctgcc ggaacaccgg gcatctccaa
      241 cttataagtt ggagaaataa gagaatttca gattgagaga atgaaaaaaa aaaaaaaaaa
      301 aaggcagagg agagcataga aatggggttc actttttggt aaagctatag catgcctatc
      361 acatataaat agagtgccag tagcgacttt tttcacactc gaaatactct tactactgct
      421 ctcttgttgt ttttatcact tcttgtttct tcttggtaaa tagaatatca agctacaaaa
      481 agcatacaat caactatcaa ctattaacta tatcgtaata cacaatggat tataaggatg
      541 atgatgataa gactagtatg cataaccgag cttccgcatt aacctcagat acagccgcca
      601 taatgactat cacgaacagt tcgcaggcgc cgattgcaat tgtaggcatg tcctgtcgcc
      661 ttccaggcca ggttcgtacg cttgatgact tctggactct catatctcgc ggtagggatg
      721 cttggggccc tatcccttct gatcgcatgt ccagtgcggc ctactaccac ccagatcccc
      781 agcgaaaggg ctgtttcaac cccaaagggg gttattacct tcaggaggat ttctctcagt
      841 ttgacgctcc gttcttccac ataacgcaac aagaagccat tgccatggat ccccaacagc
      901 gactactact cgaatgtgcc tatgaagccc tggaaaacgc cggttttccc caacaagcca
      961 cggcaggctc gcgcatgggg gtcttcacga gcctttgttc atcagactac cgtcgtggtg
     1021 cttttcgcga cctggactcc gttcccattt ttgacgccac ggggaatcag gaatctatcc
     1081 aagcgggtcg cctttcccat ttcttcaacc tccgtggccc atgtatggcg atcgatacgg
     1141 cctgttcgtc cagtctctat gccctgcatc tggcggtgca gagtattcgt tctggcgatg
     1201 cggattcagc cctggttggt ggagctagac tgcgtttgca gccagatgag atggtttcaa
     1261 tgtcgatgat gggtttatac aacgagcaag gaaaaacctt tgcatttgat gatcgtgccg
     1321 tttcgggctt tgcctgcggt gagggagtgg gctgtcttgt cctcaagcca ttagaccagg
     1381 ccctggaaga caacgatccg atctattcag ttattaggaa cacaggggca aatcatgatg
     1441 gtcgaactgt cggcatgacg aaccccaacg gagacgctca agaagaactc atgcgagaag
     1501 tctacgcccg cgccgacctc tccccgattg ataccggatt cgttgaggcg cacggcaccg
     1561 gaaccaaggt tggcgatccc atcgaagtcg gcgccatcca tcgggtgttt ggggatggcc
     1621 ggaccaagcg ggcaccgcta tacctaggct ccgtcaaatc caacttcgga catctcgagt
     1681 gcgccagcgg tattgcgtcc gtgatcaaag cgtctctgat gctccaccgt ggcttcgttc
     1741 ttcccaatgc caactttgag cgcgccaatc ccgccatccc tcttgaccaa tggaatatca
     1801 aggtgccgac caatctgcgc ccctggcccc agggcaagaa gtacatcagt atcaacaact
     1861 tcagcttcgg tggctccaac tgccacgtcg tactggaacg gcctccccgt tcgcgcggcc
     1921 agcctcccaa cccccaacac gatgcgcccc agctctttgt actcagcggc tatgatgacg
     1981 aggctgcgaa acgcattgcc aggaacctca cgttctactt ggaacaacat cccggtgtct
     2041 tcgaacggcg cctggtgcac gatatcgcat atacacttgg cgagcgacgc tctcactttc
     2101 cctggcgcgt tgctgtcacc gcttcatcta ccgcggaact tgtcgaaacc ctgaatgggc
     2161 cagcgagtgt ccctcagcgc gcccggctgg aggctggctc tgcacctgtt attgcctttg
     2221 cgtacactgg acagggcgca caatggcccc gcatgggatt ggaacttagc gacacccacc
     2281 ctatttttgc tgccaccatg gaggaagcag cacgcgcact ggaggatctg cgagcggatt
     2341 tctccctgtt cgaagagctg aagcgtgagg cagctgactc tcgcgtcagt cagccccaga
     2401 tctctcaagt agtttgcact gccgtgcagc ttgccctgac ggatcttttg gcttcctggg
     2461 gcgtacaacc gcaaatggtt gtcggccact ccagcggaga gattggggcg gcctatgcgg
     2521 tgggtgctat ttccgtgagc gaggccattg ccctggcata tcatcggggt cgactcgctg
     2581 cccaggttcg aatccgattc ccaacgttga atggttgcat gatggccgtc ggagagagtg
     2641 ccgatgaggt gcgcaaaacc atcaagcaac ttcagctagg agaggacatc actgtcgctt
     2701 gtgagaattc acctcggtcc accacggcgt ctggagatgc cactgccatg gaccttctcg
     2761 caaaggagct ggaagcgcga cagatctttc atcggaagct tatggtcgac gttgcctatc
     2821 actctcccca catgcagctg atcgccgatg agtataccag cgccatccag caggtcgcac
     2881 catccactaa gagggagaat gtccagtact actcctcgct gttaggtgaa aagatcgagc
     2941 aacccggggc tctgggagca tcgtattggg ttgataacct gacgcacccg gtccgattct
     3001 ctaccgcgct gggccagctg tgcgctgagt ccagacccga tatcatcatt gagatcggtc
     3061 cgcattccgc gttgcagggc ccgatcaagc agatactgca gggcatccag aatacgcaca
     3121 ctcaatactt tgcctccctt gtccgcaagg agcacgccac taaggctgct ctacagctcg
     3181 cgggccggtt gttcatggcc ggccagcagc tggctttccg tgaggttaac caaactcacc
     3241 tggatcagtc agtcccaaca gtgttgccgt ccctgacgcc gtatccctgg tcccatcgct
     3301 cctactggca tgagagccgc atcgccgtca accaccgact gccacgcttc cctcgtcacg
     3361 acctgcttgg acgattggaa gcctctaccc gcgaggatga acagcccgtc tggcgcaatt
     3421 ccgtgtctct ggacagcgtg ccatggctcc gcggtcatcg tatgcaatcc atgaccacct
     3481 tcccaatggc cggctacatc agcatggccg tcgaggcggc atctcaacgg gctcagctca
     3541 gaggcctctc cccgtcccag attgccgggt accggctccg tgagttccat gcttcctcgg
     3601 cgttcaccct ggaggagaac gtcgagtacg aaacctggct ctctctgagc tcgttcacgg
     3661 agggcacccg ctcctactcc agcgaatggg acgaatttcg tattgcctcg tgggcggccg
     3721 gtcggggttg gcgtgagcat tgccgtggtc tagtgggcat ccggcgccag gagtcgtcca
     3781 ctactaaccc tgttagtcaa cgccggccgt ttcatgctag cctggagcgg cgtgaggacg
     3841 ctaaacaggg tgaccaccgt cctgtatcac taaagacttt ctactcagac ctagagggcc
     3901 gcggagccgg atacagcgac gcgttcacgc tcgattcaga cagcggggtg accgttggaa
     3961 gagcaggcgt cgggaacagc tggtatgcgc gcggaaaggt tacagttccc aacaccgcgc
     4021 aaagcatgcc ctcaggccat gagaccccgt ttattgtgtc tcctgcgttc ctggatctat
     4081 ttttccagtt gtccttcccc gttttgcggg cggactctgc caacattccg tgcctcttca
     4141 tgccgacttc tatccgggag atcaatatcg cagccaattt cccatcgcaa ccgggagaaa
     4201 cggcggaggc cctagtcaat ggactgccgg cagattcctc ccgtcctcgg ccattggact
     4261 atacccttga tgcatggtct ccgtctgcgt ccacagagca acccgtcctc actatccaag
     4321 ggttccgaat gacccctgtc aatggcgata tgctgattga caccaccccg tatccgtggt
     4381 gctacactgt acagtgggag ccactgcctt ctgcaggtga agcaaatggc acggcccaaa
     4441 gtgtcccaga gagtaatgga catggcgaga cgaatggcaa tggcattgtg catgcgaacg
     4501 gcaatgatgt tgttcatggc aatggcaatc acaatgcatc taatggaagc tgccatgtgt
     4561 catcagaaga cgagtcaacc gtcatcatca tcactgagag gcccagctct gatccactta
     4621 tcgccactct cctgacagct attgatgcct tcacggggta cagaaccacg gtcgcccccc
     4681 tctctcgcgt ccagatctcg tccaaggatc gatacatctg cctggctgag ctcgatactc
     4741 cacttctgca gtcactgacc ccggaatcct tcaaacatgt ccaagagctc ctcgtcacgt
     4801 gctcctactg cctctgggtt acctccggcg catacatggc tgtcgatcat cccgagctaa
     4861 acattgttca aggtctgctg cgttctgtgc gttcagaaac cggaaactca gctgtcagcc
     4921 ttgacctgga tcctagttct cgccaagaca tcgcaggtcg ggctcatcta atccttgatg
     4981 ccttcaaagc gtccaccaac gctgtccaga cagccgccga tgacgaggag gaagcgcctc
     5041 tatccgatta tgagttcacc gaagtcggtg gacgcctgat ggtccctcgc ctagtagagc
     5101 attcagaatt gaacaatacc gtgtttcaag agacacaccc atctcttccg tacattcagg
     5161 acttcgaaca aggaacggat cgccgactca agattgccgt cggcaccctg ggcgccctgg
     5221 actcactcta ctggaccgat gatccggccc agccgctcgg cgccgacgag atcgagatca
     5281 aggtggcctg cacaggcgcc aacttcaagg atgtggtaat tgccatgggc caggtcgcca
     5341 gtccttactt gggcatcgag tgcagtggca cggtggcgcg cgtcggagcc aaggtcagtt
     5401 ccctcaagcc cggagaccgc gtatgcgcca tgtcccacgg cgcgtatagc acctacgcgc
     5461 ggtgtccggc aaccagcgcg gctgtgatcc cagactcaat ggactttgag acggcagctt
     5521 ccatccccgt tacctacagc acggcttact atggacttgt cgagttggct cacatggaag
     5581 atggggaaag tgtcctcatc cacgccgcgg cgggaggcgt cggtcaagcc gccattcagc
     5641 tggctcagct aatgggagcc gagatctacg ccacggttgg tagtgaggag aagaagcaga
     5701 tgctgatcga tcgatactgt atcccggcga gtcgcatttt ctatagtcgc aacaccgagt
     5761 ttggcccttg tgtccgcgaa gccacgggcg gacgcggcgt ggacgtggtt ctgaactcac
     5821 tggcgggcga tttcctgcgc gagacgtggg attgtctggc tccgtttgga cggttcattg
     5881 agattggaaa acgggatatt acttccaata ctcggctgga gatgggcaag ttcgagtaca
     5941 actgcacgtt ttcctccttg gacttgacgc ttgttgcgga ccagaagggc aaggtcctca
     6001 accgtgtatt taccgctgtc atgaggatgt tcgcacaggg aagactgacc ccggtatatc
     6061 cagtgacgac ggtaggtatc gcggaggtgg aatcggtgct gcgcaagttg cagagtggca
     6121 agacgacggg caaggtcgta gtggatcatc ggattaatgg gaagatcaag gccacacatc
     6181 ccctaccgtc gagtgatcta ctttcaccgg atgctacgta cattgttgtc ggtggcaccg
     6241 gcggtattgg ccaatcgatc actcgcagaa tggttcagcg cggtgctagg catattgtcc
     6301 tgctctctcg cagtggcgcc gtcacggaag ccgcccagaa aatcatcagc gacggccaag
     6361 ccgtggatgc atcgatccag atcaaaaaat gcgatgtgtc cgaagtttct caggtcaagt
     6421 cactgattgc agaactacag aaagagctac caccagtccg gggtgttatt cacgcggcca
     6481 tggtcctcaa ggacgttttc ctggagcaaa tgacattccc agactgggaa gccgtcctcc
     6541 gtcccaaggt cgcggcagcc tggaacctcc actacgcgct tctccaccaa ccactggact
     6601 tcttcgtcat gctttcctcc gtgtctggca tcgtcggcag tcgcggccaa gccgcctacg
     6661 cagcgggaaa ctgcttcctg gatgcattgg cacgctaccg ccgccaaaag ggcctatccg
     6721 ccgtatccat tgacttgacg gcagtaggcg acatcggggt attctcggaa gcagcagaca
     6781 agaaaaaggc agaagtgctg aggaacctgg cgatgagcaa cacaatgcga gaagttgaag
     6841 tactagctct gatcgagctc gcgatgaagg gccagcaagt cccagaacag tgcatcaccg
     6901 gtgtgcattg gccttcctcc gccgcaactc cgtactatgc ttctgatgcc cgcttcaccc
     6961 atcttgtcga gaccgcgaag caggaggaag gcgctgatgc cacggcccat gccggagcaa
     7021 ctaaatctct ctctatcacc cagcaagtcc gccgcgccgc gaacctgcag gaagccctcg
     7081 atgttacggc ggtcggtcta cgtgataagt tgggtgacat tctcatgctt ccccgagaag
     7141 tggtggaagc gcatacgcag tcgactccca ttgcgtcgtt tgggctggat tcattgaatg
     7201 caattgagct gaggaattgg attggcaagg agctgaaggg gcatttgcag gttttggagt
     7261 tgttgtcggc gggaactttg gggtcgttgg cgttggtggt gttgaagaag tcgagtttgg
     7321 agggggcgtg gaagggagaa atcccaaact aaggagattg ataagacttt tctagttgca
     7381 tatcttttat atttaaatct tatctattag ttaatttttt gtaatttatc cttatatata
     7441 gtctggttat tctaaaatat catttcagta tctaaaaatt cccctctttt ttcagttata
     7501 tcttaacagg cgacagtcca aatgttgatt tatcccagtc cgattcatca gggttgtgaa
     7561 gcattttgtc aatggtcgaa atcacatcag taatagtgcc tcttacttgc ctcatagaat
     7621 ttctttctct taacgtcacc gtttggtctt ttatagtttc gaaatctatg gtgataccaa
     7681 atggtgttcc caattcatcg ttacgggcgt attttttacc aattgaagta ttggaatcgt
     7741 caattttaaa gtatatctct cttttacgta aagcctgcga gatcctctta agtatagcgg
     7801 ggaagccatc gttattcgat attgtcgtaa caaatacttt gatcggcgct atgcggccgc
     7861 caccgcggtg gagctccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcga
     7921 gactcaaaca agatatattt tgcccgcctt acagacgaaa ctagttggag gttatggagc
     7981 atactatcac gtgggcggcc actggtgagt tactacaccc caggggcaac gttgatgctc
     8041 ctaaaaaact ctggctggac gcaagccgta acacccgtgt cacttcataa tcgtttgcaa
     8101 ttcagggctt gatctacaca ttaccctgtt atccctatta attaatacgg ttatccacag
     8161 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     8221 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     8281 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     8341 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     8401 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     8461 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     8521 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     8581 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     8641 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     8701 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     8761 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     8821 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     8881 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     8941 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     9001 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     9061 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     9121 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     9181 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     9241 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     9301 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     9361 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     9421 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     9481 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     9541 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     9601 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     9661 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     9721 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     9781 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     9841 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     9901 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     9961 gggttccgcg cacatttccc cgaaaagtgc cacctgaacg aagcatctgt gcttcatttt
    10021 gtagaacaaa aatgcaacgc gagagcgcta atttttcaaa caaagaatct gagctgcatt
    10081 tttacagaac agaaatgcaa cgcgaaagcg ctattttacc aacgaagaat ctgtgcttca
    10141 tttttgtaaa acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag aatctgagct
    10201 gcatttttac agaacagaaa tgcaacgcga gagcgctatt ttaccaacaa agaatctata
    10261 cttctttttt gttctacaaa aatgcatccc gagagcgcta tttttctaac aaagcatctt
    10321 agattacttt ttttctcctt tgtgcgctct ataatgcagt ctcttgataa ctttttgcac
    10381 tgtaggtccg ttaaggttag aagaaggcta ctttggtgtc tattttctct tccataaaaa
    10441 aagcctgact ccacttcccg cgtttactga ttactagcga agctgcgggt gcattttttc
    10501 aagataaagg catccccgat tatattctat accgatgtgg attgcgcata ctttgtgaac
    10561 agaaagtgat agcgttgatg attcttcatt ggtcagaaaa ttatgaacgg tttcttctat
    10621 tttgtctcta tatactacgt ataggaaatg tttacatttt cgtattgttt tcgattcact
    10681 ctatgaatag ttcttactac aatttttttg tctaaagagt aatactagag ataaacataa
    10741 aaaatgtaga ggtcgagttt agatgcaagt tcaaggagcg aaaggtggat gggtaggtta
    10801 tatagggata tagcacagag atatatagca aagagatact tttgagcaat gtttgtggaa
    10861 gcggtattcg caatatttta gtagctcgtt acagtccggt gcgtttttgg ttttttgaaa
    10921 gtgcgtcttc agagcgcttt tggttttcaa aagcgctctg aagttcctat actttctaga
    10981 gaataggaac ttcggaatag gaacttcaaa gcgtttccga aaacgagcgc ttccgaaaat
    11041 gcaacgcgag ctgcgcacat acagctcact gttcacgtcg cacctatatc tgcgtgttgc
    11101 ctgtatatat atatacatga gaagaacggc atagtgcgtg tttatgctta aatgcgtact
    11161 tatatgcgtc tatttatgta ggatgaaagg tagtctagta cctcctgtga tattatccca
    11221 ttccatgcgg ggtatcgtat gcttccttca gcactaccct ttagctgttc tatatgctgc
    11281 cactcctcaa ttggattagt ctcatccttc aatgctatca tttcctttga tattggatca
    11341 tactaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
    11401 tttcgtctcg cgcgtttcgg tgatgacggt gagcgcgttt cggtgatgac ggtgaaaacc
    11461 tcggcgcgcc attaccctgt tatccctaat atacaacgca aagaagctgg aaaattggcg
    11521 agggaatcct gtttctgtct atccaagaat gggcatgagg tggcaaccgt cgtgctagcg
    11581 tacagggtgc actttgtaac catttgggac accggacact cgctgttttc gaaattaccc
    11641 tttaagcgcg ggtattgaac caggcttatg cccaagatcg tagcaagcat ttaactgtgg
    11701 gaatactcag gtatcgtaag atgcaagagt tcgaatctct tagcaaccat tatttttttc
    11761 ctcaacataa cgagaacaca caggggcgct atcgcacaga atcaaattcg atgactggaa
    11821 attttttgtt aatttcagag gtcgcctgac gcatatacct ttttcaactg aaaaattggg
    11881 agaaaaagga aaggtgagag cgccggaacc ggcttttcat atagaataga gaagcgttca
    11941 tgactaaatg cttgcatcac aatacttgaa gttgacaata ttatttaagg acctattgtt
    12001 ttttccaata ggtggttagc aatcgtctta ctttctaact tttcttacct tttacatttc
    12061 agcaatatat atatatatat ttcaaggata taccattcta atgtctgccc ctaagaagat
    12121 cgtcgttttg ccaggtgacc acgttggtca agaaatcaca gccgaagcca ttaaggttct
    12181 taaagctatt tctgatgttc gttccaatgt caagttcgat ttcgaaaatc atttaattgg
    12241 tggtgctgct atcgatgcta caggtgttcc acttccagat gaggcgctgg aagcctccaa
    12301 gaaggctgat gccgttttgt taggtgctgt gggtggtcct aaatggggta ccggtagtgt
    12361 tagacctgaa caaggtttac taaaaatccg taaagaactt caattgtacg ccaacttaag
    12421 accatgtaac tttgcatccg actctctttt agacttatct ccaatcaagc cacaatttgc
    12481 taaaggtact gacttcgttg ttgtcagaga attagtggga ggtatttact ttggtaagag
    12541 aaaggaagac gatggtgatg gtgtcgcttg ggatagtgaa caatacaccg ttccagaagt
    12601 gcaaagaatc acaagaatgg ccgctttcat ggccctacaa catgagccac cattgcctat
    12661 ttggtccttg gataaagcta atgttttggc ctcttcaaga ttatggagaa aaactgtgga
    12721 ggaaaccatc aagaacgaat tccctacatt gaaggttcaa catcaattga ttgattctgc
    12781 cgccatgatc ctagttaaga acccaaccca cctaaatggt attataatca ccagcaacat
    12841 gtttggtgat atcatctccg atgaagcctc cgttatccca ggttccttgg gtttgttgcc
    12901 atctgcgtcc ttggcctctt tgccagacaa gaacaccgca tttggtttgt acgaaccatg
    12961 ccacggttct gctccagatt tgccaaagaa taaggtcaac cctatcgcca ctatcttgtc
    13021 tgctgcaatg atgttgaaat tgtcattgaa cttgcctgaa gaaggtaagg ccattgaaga
    13081 tgcagttaaa aaggttttgg atgcaggtat cagaactggt gatttaggtg gttccaacag
    13141 taccaccgaa gtcggtgatg ctgtcgccga agaagttaag aaaatccttg cttaaaaaga
    13201 ttctcttttt ttatgatatt tgtacataaa ctttataaat gaaattcata atagaaacga
    13261 cacgaaatta caaaatggaa tatgttcata gggtagacga aactatatac gcaatctaca
    13321 tacatttatc aagaaggaga aaaaggagga tgtaaaggaa tacaggtaag caaattgata
    13381 ctaatggctc aacgaaatga cggtatcgat aagcttgata tcgaattcct gcagcccggg
    13441 g
//
