LOCUS       sequence-212417-       14866 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14866)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 14866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHSU72.1-2.2
FEATURES             Location/Qualifiers
     source          1..14866
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     466..482
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     466..482
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(729..1823)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(1824..2231)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     primer_bind     complement(2526..2544)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      2585..3927
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    2917..2964
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        3954..4058
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4059..4127,4128..4919)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4277..4296)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5090..5678
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5579..5598
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6015..6035
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6017..6035
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             13309..13320
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(14798..14821)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctttttgtag cttgatattc tatttaccaa gaagaaacaa gaagtgataa aaacaacaag
       61 agagcagtag taagagtatt tcgagtgtga aaaaagtcgc tactggcact ctatttatat
      121 gtgataggca tgctatagct ttaccaaaaa gtgaacccca tttctatgct ctcctctgcc
      181 tttttttttt tttttttttc attctctcaa tctgaaattc tcttatttct ccaacttata
      241 agttggagat gcccggtgtt ccggcagagg agatcagtct cgtgaagtgg atggtttccc
      301 gcctgcgggc aaaacgtcat aacattttta tgagcgaaag ccgttaatga agacaaaatc
      361 ccttaattaa aacattagaa tggtgattag aaaggcagga ttaatcagtt acacaggctg
      421 taaccggaga gacggatcat aaggcaattt ttagatatta attaatctag aactagtgga
      481 tcccccgggc tgcaggaatt cgatatcaag cttatcgata ccgtcatttc gttgagccat
      541 tagtatcaat ttgcttacct gtattccttt acatcctcct ttttctcctt cttgataaat
      601 gtatgtagat tgcgtatata gtttcgtcta ccctatgaac atattccatt ttgtaatttc
      661 gtgtcgtttc tattatgaat ttcatttata aagtttatgt acaaatatca taaaaaaaga
      721 gaatcttttt aagcaaggat tttcttaact tcttcggcga cagcatcacc gacttcggtg
      781 gtactgttgg aaccacctaa atcaccagtt ctgatacctg catccaaaac ctttttaact
      841 gcatcttcaa tggccttacc ttcttcaggc aagttcaatg acaatttcaa catcattgca
      901 gcagacaaga tagtggcgat agggttgacc ttattctttg gcaaatctgg agcagaaccg
      961 tggcatggtt cgtacaaacc aaatgcggtg ttcttgtctg gcaaagaggc caaggacgca
     1021 gatggcaaca aacccaagga acctgggata acggaggctt catcggagat gatatcacca
     1081 aacatgttgc tggtgattat aataccattt aggtgggttg ggttcttaac taggatcatg
     1141 gcggcagaat caatcaattg atgttgaacc ttcaatgtag ggaattcgtt cttgatggtt
     1201 tcctccacag tttttctcca taatcttgaa gaggccaaaa cattagcttt atccaaggac
     1261 caaataggca atggtggctc atgttgtagg gccatgaaag cggccattct tgtgattctt
     1321 tgcacttctg gaacggtgta ttgttcacta tcccaagcga caccatcacc atcgtcttcc
     1381 tttctcttac caaagtaaat acctcccact aattctctga caacaacgaa gtcagtacct
     1441 ttagcaaatt gtggcttgat tggagataag tctaaaagag agtcggatgc aaagttacat
     1501 ggtcttaagt tggcgtacaa ttgaagttct ttacggattt ttagtaaacc ttgttcaggt
     1561 ctaacactac cggtacccca tttaggacca cccacagcac ctaacaaaac ggcatcagcc
     1621 ttcttggagg cttccagcgc ctcatctgga agtggaacac ctgtagcatc gatagcagca
     1681 ccaccaatta aatgattttc gaaatcgaac ttgacattgg aacgaacatc agaaatagct
     1741 ttaagaacct taatggcttc ggctgtgatt tcttgaccaa cgtggtcacc tggcaaaacg
     1801 acgatcttct taggggcaga cattagaatg gtatatcctt gaaatatata tatatatatt
     1861 gctgaaatgt aaaaggtaag aaaagttaga aagtaagacg attgctaacc acctattgga
     1921 aaaaacaata ggtccttaaa taatattgtc aacttcaagt attgtgatgc aagcatttag
     1981 tcatgaacgc ttctctattc tatatgaaaa gccggttccg gcgctctcac ctttcctttt
     2041 tctcccaatt tttcagttga aaaaggtata tgcgtcaggc gacctctgaa attaacaaaa
     2101 aatttccagt catcgaattt gattctgtgc gatagcgccc ctgtgtgttc tcgttatgtt
     2161 gaggaaaaaa ataatggttg ctaagagatt cgaactcttg catcttacga tacctgagta
     2221 ttcccacagt taaatgcttg ctacgatctt gggcataagc ctggttcaat acccgcgctt
     2281 aaagggtaat ttcgaaaaca gcgagtgtcc ggtgtcccaa atggttacaa agtgcaccct
     2341 gtacgctagc acgacggttg ccacctcatg cccattcttg gatagacaga aacaggattc
     2401 cctcgccaat tttccagctt ctttgcgttg tatattaggg ataacagggt aatggcgcgc
     2461 cgaggttttc accgtcatca ccgaaacgcg ctcaccgtca tcaccgaaac gcgcgagacg
     2521 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     2581 gtatgatcca atatcaaagg aaatgatagc attgaaggat gagactaatc caattgagga
     2641 gtggcagcat atagaacagc taaagggtag tgctgaagga agcatacgat accccgcatg
     2701 gaatgggata atatcacagg aggtactaga ctacctttca tcctacataa atagacgcat
     2761 ataagtacgc atttaagcat aaacacgcac tatgccgttc ttctcatgta tatatatata
     2821 caggcaacac gcagatatag gtgcgacgtg aacagtgagc tgtatgtgcg cagctcgcgt
     2881 tgcattttcg gaagcgctcg ttttcggaaa cgctttgaag ttcctattcc gaagttccta
     2941 ttctctagaa agtataggaa cttcagagcg cttttgaaaa ccaaaagcgc tctgaagacg
     3001 cactttcaaa aaaccaaaaa cgcaccggac tgtaacgagc tactaaaata ttgcgaatac
     3061 cgcttccaca aacattgctc aaaagtatct ctttgctata tatctctgtg ctatatccct
     3121 atataaccta cccatccacc tttcgctcct tgaacttgca tctaaactcg acctctacat
     3181 tttttatgtt tatctctagt attactcttt agacaaaaaa attgtagtaa gaactattca
     3241 tagagtgaat cgaaaacaat acgaaaatgt aaacatttcc tatacgtagt atatagagac
     3301 aaaatagaag aaaccgttca taattttctg accaatgaag aatcatcaac gctatcactt
     3361 tctgttcaca aagtatgcgc aatccacatc ggtatagaat ataatcgggg atgcctttat
     3421 cttgaaaaaa tgcacccgca gcttcgctag taatcagtaa acgcgggaag tggagtcagg
     3481 ctttttttat ggaagagaaa atagacacca aagtagcctt cttctaacct taacggacct
     3541 acagtgcaaa aagttatcaa gagactgcat tatagagcgc acaaaggaga aaaaaagtaa
     3601 tctaagatgc tttgttagaa aaatagcgct ctcgggatgc atttttgtag aacaaaaaag
     3661 aagtatagat tctttgttgg taaaatagcg ctctcgcgtt gcatttctgt tctgtaaaaa
     3721 tgcagctcag attctttgtt tgaaaaatta gcgctctcgc gttgcatttt tgttttacaa
     3781 aaatgaagca cagattcttc gttggtaaaa tagcgctttc gcgttgcatt tctgttctgt
     3841 aaaaatgcag ctcagattct ttgtttgaaa aattagcgct ctcgcgttgc atttttgttc
     3901 tacaaaatga agcacagatg cttcgttcag gtggcacttt tcggggaaat gtgcgcggaa
     3961 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     4021 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     4081 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     4141 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     4201 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     4261 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     4321 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     4381 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     4441 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     4501 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     4561 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     4621 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     4681 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     4741 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     4801 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     4861 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     4921 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     4981 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     5041 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     5101 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     5161 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     5221 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     5281 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     5341 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     5401 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     5461 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     5521 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     5581 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     5641 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     5701 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     5761 attctgtgga taaccgtatt aattaatagg gataacaggg taatgtgtag atcaagccct
     5821 gaattgcaaa cgattatgaa gtgacacggg tgttacggct tgcgtccagc cagagttttt
     5881 taggagcatc aacgttgccc ctggggtgta gtaactcacc agtggccgcc cacgtgatag
     5941 tatgctccat aacctccaac tagtttcgtc tgtaaggcgg gcaaaatata tcttgtttga
     6001 gtctcgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctccaccgcg
     6061 gtggcggccg catagcgccg atcaaagtat ttgttacgac aatatcgaat aacgatggct
     6121 tccccgctat acttaagagg atctcgcagg ctttacgtaa aagagagata tactttaaaa
     6181 ttgacgattc caatacttca attggtaaaa aatacgcccg taacgatgaa ttgggaacac
     6241 catttggtat caccatagat ttcgaaacta taaaagacca aacggtgacg ttaagagaaa
     6301 gaaattctat gaggcaagta agaggcacta ttactgatgt gatttcgacc attgacaaaa
     6361 tgcttcacaa ccctgatgaa tcggactggg ataaatcaac atttggactg tcgcctgtta
     6421 agatataact gaaaaaagag gggaattttt agatactgaa atgatatttt agaataacca
     6481 gactatatat aaggataaat tacaaaaaat taactaatag ataagattta aatataaaag
     6541 atatgcaact agaaaagtct tatcaatctc ctcagaagaa gttatcgaga atggtatcaa
     6601 gacccgactt gggagagaga ataccaaaat gtgtaccagc caaatcatac ttctccacgt
     6661 ttggggcata aagatccagg tcgaagtact cggaagcacc ggcggcgcaa ggctgggcgt
     6721 atacaccttc caaagcgcgc acgaggactg tcctcttcgc aggccactct ccagtgatcc
     6781 tttccccaga agcagcttcc tccgcgttga cttctttacc gcggcgaaca cccattagga
     6841 cgtgagcgac ataggcagtg cagaaacgag cgatccactt gggtccaccg cttccaccgt
     6901 ctttggaatg ttgaaagtga tctgcgaact ggtagctcaa gtgctcggag ttatcgagtg
     6961 ttccgctcga cgccatatcg ttgacaatac cgaggatatc ttcgcggaga gtgccatctt
     7021 ggattcgtcc catgatgtat gattggaatg caggtcgctt gaagagagtc ggggagccat
     7081 cgagaagaac taaggcttcc acaacttcac cccgcttctg taacaattcg gccatatcca
     7141 acgcaaggca tgtaccgaag gagaatcctc caaggcgata aggaccatgg gactgcttct
     7201 cctggatttt cttaaggtag aattcggaga gtcggcgtaa ggtgccggtg ataggtgctt
     7261 ccacagtgtc ttgcacaccg tagacagggt aatgagcctt ctgggcagct ttgacaagga
     7321 cgaggatgcc tccaccagca ccgtgcatga ggaagagagg agtcccttcg gttgcggtgt
     7381 tgagtcgaac aacggtctgg tccaaatcag cttcgtgaac gttggcctgc tcgccctcag
     7441 aagggccgtt ggcagtgaca gtggtagcag cagaagtttt ctgctcttct tgaagctgat
     7501 acaaccgagc gacagtcatc gtttgactga ggagctgcaa ctgagtaacc tccacaccaa
     7561 agtaagtcct gaggatacca ctcagacgta cagacgtcag cgaatccaaa ccgtaggtcg
     7621 agaaagggat gttgtcttcg actcggtcga tgtcaaggga caaaaccgag gcgcaagctc
     7681 cgagaattga ctgctccttg gcaccgttgg aggttgcacc gtcgctagtt tccttgacca
     7741 aaagatgtcg catgctggcc tggtgatact ctggaacggc aatttccata actcgcttcc
     7801 aattggtcgt agggatgtat acagggttat aagccggtga gttgagagtc cagattgcgt
     7861 ccacacagtg gcgcgccagc ttgtaaccac tcattccgag atctttgtat ttgtccagcg
     7921 cagcacttcg agcgtttcca gtcggcatac ttcggacgaa gacaccacca tcagtcaaag
     7981 gaggaacagt aatggtaaca cagttaggaa tatccgcgcc cattgcctcc atctcggttt
     8041 gagccgcagc atagttgctt tgaccaggag aaccactgat tgtggccata gaactagtca
     8101 agacaaggaa gtctagcttt ttggggtcga cggcatctag gagaatacgc aagcccttga
     8161 ctttgacatc atacactttc agccagtctt cctcggtagt gaggtttagg aaaagagcgt
     8221 cattgagtct cacggcaacg tagaagacac cggcgacatt cggtaggttg gagaatagag
     8281 ccttagtctt cgattcgtca agacagtcca ctgcgacgac gtcaacctta gcaccggttt
     8341 cgcgcaagta attgataatt ttcttctcgc gaagaagctt gccattctcg aatgccttct
     8401 ctcctgaacg cgaggtcaaa acgacatgtt gtgccccaga ttcaaccaac agccttgcaa
     8461 tgttgacccc aagaccaccg attccaccaa taatgacata agcggcacat gggttgaacg
     8521 cgttcgagcc cttgattcta ccgggtcgca gaaccgtcgt gttctcgggt aacgcttcaa
     8581 aattgagaag gacagtcata tccctgtaag gcgccgcctt ggaatgggca agctctaacg
     8641 catcagcttg cacaatgata aaaggtggcg aaagacggga catgatgtgg cgtggagtgg
     8701 ttcggatagc ctcttccaaa atgttctgtg ctccagagaa gagtcggtca tacccaacag
     8761 aaacgaaatg ttgaccagga cggagatgac aagggtagat accgccgatc tgcacaatgg
     8821 ttccccaagc agaaagccgc tcgatggact cttcaaggat gtcatttcca tcttcgaagt
     8881 tgaagacgaa atcggcaccc tctggtgccc atctctggac agcatgggac cacgcttgca
     8941 cgttacctga ttcgaaaacg tctttgtgag ggataggtgc cgagggaccg gagattgtga
     9001 caaagatgtt gaaaccttgc tcttggaggt aggcaagggt agccagagcg gcaggcgagg
     9061 attcagcggc atggaggatg acgtgagagc tggacttcgc agaagccaaa attttcatta
     9121 cgaccgagga gatgaacgcc aaccgaccaa caagagcagc agaggacgga agactcatgc
     9181 tgtcggggat gaccgttacg gaacccttgg gacatatgat gaagttgccc ttgggggtgg
     9241 aggtcactcc catgacccgc ttcccagtta acgactcgtc gacgttagcg ccaacatgga
     9301 cgaccttccc agagaactca gagtgtcctg gaatcacact cgagagggca atgaaagata
     9361 cggcgacttg aacttcgtct gcatccaagg ttttggggaa agtgcgccag aaccgagtgc
     9421 catcgaagtg taagggttta gagccgacgg tttcggtaga aggcggtaag ggaccggcaa
     9481 caacacgtgg cactcgcatg gcaccatctt cctcgaccat cacttcggca tcaacccatc
     9541 ggagaggaat gagctccttc cgaacgaact cctcttgttt aactggggtc caagatgggt
     9601 ggaaaaggat caagtagatc ttccagaggt caaactcgtg acggagtgat cgagtcaggc
     9661 caatgagtgt tgcattggtg gctttggtgt cggtgtataa ccaaaccttg agcggcgtgg
     9721 atgaatcaag tccagcgacc aagttgacca attcaacttc tccgcctgcg gtgaagcggt
     9781 gagtgacagt gttcggcgga agggtgaggg attggacttc ctctagggta ggtagaacaa
     9841 tttgcggctt ctgtttctca actgctggat cctccgtcct gtgcttgctt ctttggagaa
     9901 gaagcagcag aggaatcagc cagagcgatt tcaacaacga gtacttatca atgatggtga
     9961 gtatgccttg tagatgatca tcctgcaagc catatgtagg aagataaggt gtggcaacag
    10021 taggggagga gccgctatca ctctgaagtg atggggtgga aattgcgggg gagctgggtt
    10081 tgcaaactgc catctccgca gtttgcgaga agaatgccat gtgcgacact gtggtgccag
    10141 atgttgtcaa gaagaggcca tcagagaagc ctgaggtctt tagagcgacg ttccactcgg
    10201 acttgtccaa agaacaatga gaagcaggac gatgctctag aacgccaaac cattcctgga
    10261 atgaaccgaa gatgtaatcc atccaaacac ttccaacagc gccagttgca aaggatcttc
    10321 catccaattc aatgatggcg aggtggccgc caggtagaag caaatcatga agggtcgaga
    10381 gtgtggaggt gatgctcggg gttgcatgca gaacgtcgaa agcaacaaca atgtcgaagc
    10441 ttgccggatc aataccttgt tgttcaatag gcttattgag atcaaatgtg accggaacga
    10501 tagtggccca tggtgacttg gcagtagctt cttgagcaag cgaaattgag atatctgtac
    10561 agacgtagtc cacatagcag agctcaagcc tcgcgtccag caaagcttgg ccaagcatag
    10621 ctgtgaatcg accagtacca gcaccaacct ccaatacacg aatgacccgt ttaccagccg
    10681 cttgtgcagt tcgaacgagc ttcacaaact gctcgacagt ctcatcgaag acgcttccaa
    10741 tgaaaggagg atgctcataa atacggctca tgagatcgtc acggaacagg atgtcaacgg
    10801 cagctttgga gttgacgaga atgtctgtct ggccttctcc aacacgctga gtgagttcaa
    10861 aaagggaggc atatttctcc ttcattgcgg gtgaaacatc gtcaatggcc atcgtggact
    10921 cgagtgcata gcccgacgtg cgttgggctg cccatgatac ataccgcttg cgatccggaa
    10981 gagtgggctg gaaatcttga ggaaggcggc tcaacgcctt ttttgtgtaa cccaaagcca
    11041 gcttgtccag tacttctaga agttctgcga cctcggcaga gaattcatct gcgagggtga
    11101 catcaccgtg aagatgggat tcagggagag cacgaggctg ccaaatcatg ctgaaatgct
    11161 cattgacggt agcatcatct tgctgtacca tctcaaagtg gaggccttcg aaggtgaata
    11221 caacgtcatt gttatcatcg agcacgtagc agtccaaagt ccacgagtac ggtgaccagt
    11281 aagcgaggac agcgtagact cggaagtcat catcaaaaat aacagggtcg gtagtcccat
    11341 tgttgcggaa gccacgacgg agagagtgag gtaacatgaa ggtccgctct ttgttgccaa
    11401 cattcacatg atcgtacagc aagttccaac tgataccgga ctggaagacg gcatccatca
    11461 gggcgggatg gaacgtatag ccggtacgag tgagagtatt gccatgacct ttgatccaaa
    11521 caatggcttc agtgtcgtta acagcaatct tctgaattcg gcggaactcg gttccaaact
    11581 gtgcgacacc ctccaactca tcgtaaacag cctcatgaag atgtgttgac cgagaacgag
    11641 caaggacagc gtcgacgtcg acgtgtgtga tacctcccgg gctgatctct ggctgaccgt
    11701 agtcaagctt gccgtaagcg tgaagggaat cgaagggtgg ttcagagcga gtccagttga
    11761 gatctccttg catactctgg agagctgtag acgacttcac gctccacctg ttgccatcac
    11821 gagcaacctt gagagtagct gcaggcacgg atccatctag aataaatgcc gactcgaacc
    11881 gacaatccca gactgatgta acgtcgggga actcgagggc catttcgatg tatgcggcag
    11941 caggaatgag gttcgattga tcgatgacat ggtcagccat ccaagtttcc ggaagactcg
    12001 ggcttacacg taaccgctcg ctgttgagag gcctttcagg aggcagcaat ttgtggaggt
    12061 agctgggaac gcgaggcgca atggtaaagt acttcgattg gaaggggtat ttcggaccga
    12121 ggagcttctc tggaggacag ccgttgagga gtgcaaagtt gatcgagttg acccctgcaa
    12181 ccgacagctg accaatccct tcgtacagtg catcaagttc ggtgggaggg cgttgaccag
    12241 ctttgatgtg acgagccgaa ggaggacgtt tgctacccgc gactgattcg aaggcaccca
    12301 ttcgtttaat gtattgagac aagacgggat gaggagcaat ctcaacgaag gtcgtgtttt
    12361 cgccgtactt ctcgatgagc ttgggaatag catcagagaa gcgaacaggt tgacgaaggt
    12421 tgttccagag gtagtcgatc gtgtactcct tctcaacgaa ttcggctgtg actgtcgaca
    12481 tcactggaag cgaaggcttg tgggtaccag ggtgtgcttc aaagattgca gcgagctctt
    12541 gacggtattg ggtttcgcaa gcatcgacat aaggagagtg aacagcagtg ctgacacgaa
    12601 gcttgcgagc agatacccca gaaacccatt ggtcgatata cttggccagt tcgtcgacga
    12661 gatactcagc acccgacaga ccaaagtcgg tcgggctatt ccgagccgcc acgtggagag
    12721 agtcggtaac acccgtcgtc gggttattgg aaaggtcaac gactgcttcg gcataatctt
    12781 ggactgttcg aaggtcgcaa ccagagacag cgaccatggc tccaccaatg ttgtcaacga
    12841 ggcgaagagc agcacctcga gcaacggcga tcttgacgac catctcgcga ggcatagcac
    12901 cactggcata aagaacagca gtttcgccga tactgtgtcc aacgatggcg tcgggcttca
    12961 gaccaaggta gatcgaaaag gtcgaaaaga gcgatttggc gaggatagag ttcgcaggag
    13021 ggttggggac aaagaggccg gaggtttcaa ggaaggactt tcctgtgtac tcggtgtaaa
    13081 ttttgtcgga agccaagata ctttcacgga aaacagagta tcgggagaag agttcacgac
    13141 cctgttgcca gtgctgaggg ccttgaccag agaagcaaaa gatcagagga acagcacgtt
    13201 ttgataccat gacaggttca gtgaactttg cctggcccaa ggaatctgca actgcgaatg
    13261 aacgccagct caattggcga gcacgagagc cgaggtgttc agatagggat agagggtcga
    13321 catccgcgta ctcggctttg tagctctcga cgagaatgtt gactgcacga ggagtcagac
    13381 ctccaacggc gaagagatat ggtcccgagt ttagagtttc atagtcaggg aggacgggcc
    13441 taacatcgtg ttctcgcagg acagtgtgac cacaagatcc tccaaatccg aaggaagaaa
    13501 tcgacgcaat aagaccttgc tcggcgtcgt agggaacaaa gttttcgact ttaataggta
    13561 cccgcagttt gtacttgtca aactggatct tcgaagaagg ctttttgaag tatccattcg
    13621 ggagaatctg cttgcgctcc aacatcatga caaccgagcc tgccttaaca atcgaactga
    13681 tgtatgcgcc catctcaccg tgaccgatat tactcttgac ggaaccaatg acgacctctc
    13741 tgtcaccacg accatccgag aagcaggcgc ccgcagcgtt ggcttcgaac tgatctccaa
    13801 ctatggttcc ggtaccgtgc agctcgacga aatcggcttg gttgggaact aaaccagcgt
    13861 cagagtaagc gtttcggatg gccgctgctt gagcatcagg gctgggagtg gtcaaagagc
    13921 ccataagaga accacaggaa gtaagagcgc ttccgacaag tgtagccaag atacgatcat
    13981 gatctctgac ggcgagatcg tgacgtttga taacgagagc gccaacaatg tcaccacgag
    14041 catagccgtt ggcatcttca tcgaaagact tagacatacc atctggacta agaactccga
    14101 gtctgctaaa ggaagaagtc tcgagcggac tcatgatgat gttgacacca gcgacgacgg
    14161 cctggtcaca ctcgccgttc ctaatggcct gaacggcgag atgcatggcg gtaagactag
    14221 aagagcaagc cgtgtcaaca ggcaaggacg gtccggtcat gtcgaagaca tagctgatac
    14281 gattagcgtt gattgcgaga gaagtacctg tcatgtagta cgctccagcc tcagtaatat
    14341 ccatgtcaaa gggagcacct ccgccggcgc atccgacgta gacgccagta ttagtacctc
    14401 tgaagtcaac gccgctacgc tgaagggcgt tgaaggcctg gtgaagcatg gcaatctggg
    14461 agcaagacat ctgggctgat tcagccgggg taataccaaa ctcttgaggg tcgccgatgg
    14521 tgaaattggg tatatagccg cccttagtag cggcaatctt gcctggttga cctggctcag
    14581 tagcacggta tgcctcggcg ttccaacggt cctttggcac ggtaatgata ccagaaccac
    14641 ggaccttcag gaactcatag aactcatcac agtcaagtcc gtcgtcaacg ccaccagggg
    14701 cggcgacaga aacaccgatg atggccatag cgtgagtatc gatgtgatca tagccaggac
    14761 ggaagccagt ggcagttggg gttgaaacca tactagtctt atcatcatca tccttataat
    14821 ccattgtgta ttacgatata gttaatagtt gatagttgat tgtatg
//
