LOCUS       sequence-212418-       13252 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13252)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 13252)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHKU39.1-2.2
FEATURES             Location/Qualifiers
     source          1..13252
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(7165..7185)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7165..7183)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(7522..8110)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7602..7621)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8281..9072,9073..9141))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8904..8923
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9142..9246)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9273..10615
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(10236..10283)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     10656..10674
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10969..11376
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             11377..12471
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(12718..12734)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12718..12734)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             join(13245..13252,1..16)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ggatgatgat gataagacta gtatggcacc tacgaccaca ttcatcaatg ttcctgtctt
       61 cgccggtcac ggtacgactg ccatggctgc gtcttccact cgtgaacagg ccattgctga
      121 tgcttctcac ccatctggcg ctttgcttct cagctcgttc catcgtgctt tcctccgcga
      181 acgagcttcg ctctcatccg aagatcttga cgatttagct ctccccgact tttctactcc
      241 ccaagaattc ctctccatca tcggcgaaca gcccattagt aactcccttc aatccaacct
      301 ttctcttctc ctcgtccagg cgcttagata tctcgcccac gtggaggcag gctctttatc
      361 ggagtcactt aaccctttca ccgaattctt ggtcagcaat gtagatcata aagtcggcgt
      421 ggctgggttc tcctcgggaa tcctgcctgc ctgtgcagtg gcatgctctc aggacagcct
      481 atcctttatc aaacatgccg tagagctttt ccgctttgct ttctggttgg gacttagatc
      541 ccaacagtac cagaccaaag cgactcgtga actgaccaaa tctcatggag aaaaaacgcg
      601 tcagttttgg agtagagtta tcatgggaca atctgagtct gaagttagag ctgcaataga
      661 catcttcgct gtacagaacc ccacgcttcc gcaaatttat atcaccgcta tcagtgatga
      721 cacgacgttc acggtctctg gtcgcccgga cgctttatct gcattcatag aaacccttcc
      781 ttctacaagc cgaatattta atttgacggt ggatacatta tatcactctc cttgccacca
      841 ggacggattg cgagcgcagg ttctcgcaga tgtcacccga cgtggcgtaa tattccctac
      901 cctagataac ctaatcttcc ctctccgatc aacttctact ggtgaactcg taaacgagtc
      961 taacgacggc acaactctat tagaaaacat cctcgatatg atcgttgttc aaccagtcaa
     1021 gtggcacttg gtcacagacg ctttgtccaa ggcgccccca gtcgacattc ctattcgctt
     1081 gttgaacttt ggacccggaa caggactcac tcgttccatg gttaaagcat tttccaagga
     1141 catcgtttcc actcaagact tgacctcgga gaatgcggcc aagcgcctag aaggtgctac
     1201 gaccgaagga cagatcccca ttgccattgt gggaatggca ttgaacatgc ctggagctcc
     1261 gagtgccgcc aagttgtggg agttgctcga aaacggtatc aacaccgtct cggaggttcc
     1321 gtccgaacgc ttcaacatct cggagtacaa caagccttcg gatcccaaat ctaggcgagc
     1381 catgaaggcg cacaccggaa acttcatggc agacccaagc ctctttgatg ccaagttctt
     1441 ccgtatttct cctcgtgaag caaagagcat ggacccccag cagcgtatct tgcttcagac
     1501 cgcttacgag gctttggaga atgccggata cgtcccgaat gctacaccaa ccttccaaca
     1561 ggacaccttt ggttgttacg tcggtgttgc gactgatgac tacgttcaga atctcagaga
     1621 cgagattgat gtctactata gtactggtac ccttcgtgcg ttccttagtg ggcgtatttc
     1681 atacgcaatg ggattcagcg ggccctcaat cgtcatcgac acagcctgct catcatcttg
     1741 tgtttctgtt taccaagctt gtcgggcgtt atcgaacgga gactgtaacg cagctgtcgc
     1801 tggtggtgtt aatgtcattg caagcccgga tatgatgatc ggcttggaca gggcccattt
     1861 tttgagtccc accggccagt gcaagccatt tgacgcttct gccgacggct actctcgctc
     1921 tgaaggatgc ggattgttcg ttttgaaacg cctctctgac gccgtcgccg agaacgataa
     1981 catcctcggg gtaattcgtg gggttgaggt caaccaatca ggaaatgctc attcgattac
     2041 tcacccccat gcacctaccc aagtcaagct gttcgagagg ttgcttgaga aaactggcgt
     2101 tgataggcat cgtatcaacg tgatcgaggc acatggtact ggtactcagg ccggagatcc
     2161 aaatgagctt gaaagtattc gcaagacttt tgctacgggt cgtccaaagt cgaaccctct
     2221 gcatatcact tccgtcaagg ccaacattgg gcacctcgaa gcagcaagtg gttcggctgg
     2281 tttggccaag ctccttctca tgcttcgcca ccgcaccatc ccccggttga tctctttgaa
     2341 gaatctcaac ccactcattg ctccgttgga cttggataac actgcgattg atacagcagc
     2401 ctgcgaatgg gctccttcag agcccggtct gcctcgactc gccatgctca acaactttgg
     2461 tgctgccggt tccaatggag ctgtactgct ggaggaatat gtagcgcctc ctcgtgacga
     2521 ccccataact gcgccgatta ctttaccctt tggtttatct gccaaagatt ccattgcgct
     2581 cgatcaactg cgccagcgct atgtggacta ccttaggctt cccgagaacg aaggaacgag
     2641 cctttacgac attgcttata ccatgactgc gcgtcgccag atgtacccat tccgtatggc
     2701 tgttacagcg tcttctcgcc aggagctcgt cgagaagctt ctacaggcct ctggcactca
     2761 agcaaaagag tcgaacgccg aagtagcttt cgtattctcc ggccagggtg gtcaatactt
     2821 gggcatgggt gcggctttgt atgaaacctg ccctgtcttc aagacccacg ttgacgaatg
     2881 tcgctcatta ctatcctgta tgggcttcgg cgacatcctg tcaatcattt cgccatccgg
     2941 tgagagaagt gggctaagtg ctacagatga attggaggtt taccaaactg ctgtgtttgc
     3001 cttggaatat tccctggcac aactatggat gtcctggggc ctttctccgg tcgcagttgt
     3061 tggtcacagc ctgggagaat acgctgcctt ggttattgca ggagtcctca gtcttcggag
     3121 cgctctattt gtgatcgcat cccgagtgcg tctcatgctt cgcaagtgcg agatgaatag
     3181 cacagggatg atagcaatca ataacggccc cgctgaagtc caaaagatac tcgattcaac
     3241 tcctttgttt gaatctctca gtgtcgcctg ctacaacagc gtctcggact gcgtcgttgc
     3301 tggtcctttg acaggattga aggcactcaa gtcttatttg gacgatgaga tccgctgcaa
     3361 gagtaccatc ttgaacgttc catttggata ccattccgct gcaatgaacc cgctggtgga
     3421 tgatttgaac gcggtcttgg aaaccgtcaa acttcatccc ccgaggattc ctgtagtatc
     3481 caacgttttc ggcagtgtag tagagccagg agatggtacc gttttcactt cgacgtactt
     3541 ctcgcgtcac tgtgccgagc ctgtcaaatt ctcagatggg tttgccacac ttctggccga
     3601 cgcggaactt gctgccaatg tttggattga ggtcgggcct catccgacaa ccctacccat
     3661 gatgaaagca tgccccgctc tgccaaagga tattcgcctt cttccttcgc tcaggaagaa
     3721 ccaagaccct tggagtactc tcaccacctc attgagtacc ctgtatgcag aaaagtcgga
     3781 tgtcgcttgg aggaatgtgt tcagtcacct tggcagtgtc agctgtgtcg atttgccgac
     3841 gtacccgttc actaacgtga aatattgggt caagttcgag gagagtgaag ccactgtgca
     3901 tacagccaca ccccttccag cgcaacagtc ggtcgatacg atcaatcaat tctcactttt
     3961 gcgttcctgg gttcagcgcc catcaactgc gaatgggaac gtggcaattg tggaggttcc
     4021 catcagcgtc ctctccaagc tcattagcgg ccatcaagtt ggtgaacatc ctctgtgtcc
     4081 cgcatcggtt taccatgaac tcgcaatggc agctatcaaa cttgcgacga cgaataaacc
     4141 aggacattac atcgccttac gggatattga ctatgctaag cctttggttc atgctgagaa
     4201 cgacaccagg aatatcagga ccgttgtctc tatccctgat ggtagttgga aggtcatctc
     4261 gcacagcagc gacgaaagcg tacactgcac cggaaagttc tcctgggata acgtcggaaa
     4321 agctgccctc aaattccagc agtatagacc cattctcagc cgggaaatcg cctctgtcat
     4381 tgacaatgta gattccgaac ttttctcgac tcgtacgatg tacgacatca tcttcccgcg
     4441 tgtcgttcac tattccaaag agtatcactc cgcacgatgc cttactgttc acccttctgg
     4501 tatggagggt catgctaccg tcaaaatccc acacgcgtca acccccggtc agtacgtcgt
     4561 tcatccggtc ttcatggaca ccctccttca cgttgcaggt ttcgttgcga atatgcgagc
     4621 cggtgccaat gacgctttca tctgcagcca ggtggactct gtcaaggtgg tccctgaact
     4681 ggtcaataat gatgcgacgt acgatgtctt ctgccggaat gcatggcttc ctgaggaggg
     4741 actcattctt gcggatgcct atgccgtcac cacgggtacc tctccgaaaa ttgttgccca
     4801 tttgaagcgt atgcatttcc gcaaagtccg gctcgctagt ctcaagcgag ctttgaaaca
     4861 tgctcaaggc ggatccaccg ctccgactcg caccgccgca aagtctgcat catcggttgt
     4921 tgctgcaaag acatcagcat cgcccgccca agtcaaagtt cgaggcaagg gcgaggatat
     4981 actagatcaa gtccgaaaag ccgtcgcaga tacctgtgac gtcccgatga cttcaatgga
     5041 catttctgct gatctcacct cctttggtgt cgattctttg atgagtatcg agatatttgg
     5101 tcggcttcaa gcgttgttcc cagatgcaga actagatcca cgagtcctat ctcatctccg
     5161 taccatttcg gaaatagcag cagaagtggc gtcgaaggct ggtgatcttt caccagccgc
     5221 tacctctgag gcagagtcgt catctcctgc tccatcgact ccctccaact gcgggggcaa
     5281 tgttttagat caggttcgtc aggcagttgc ggacacttgt gacctcccag tgacctcgat
     5341 gaacatctct gctgatctca cttcccttgg agttgactcc ctgatgagta tcgagatatt
     5401 tggtcgactt caaatgctgt tcccagatgc agatctagac ccacggatcc tatcccatct
     5461 ccataccatt tcagaaatca cagccgaggt gacgtccaag gtcggggatg cttcgctggc
     5521 tactacccct gagtcttcgg gaacagctgt tgaggatgtc gttgcagata ctatggtcat
     5581 ggaagagcaa ggagccgatg tgaagacggt aatagcaggg gtgcttggta tcggcgccga
     5641 cgaattcaac gacgacactg acttggagtc gctcggtctg gattcactga catctattga
     5701 agttatgcaa gcagtgcagg acaacctctc catctctatt cctcaacacc ttttggcctc
     5761 tcatcccaca gttcgagcaa tcaacgaatt tgtttctagt caacggaagc cgccaaaaac
     5821 cgaagcacaa agtggtaacg tcgccaaggc tccagtgctt tctgccgccg ccttgaaagc
     5881 tcttgcgctc gatgaaatgc cagccccgct tatacgagat ccaacatccc agaggacccc
     5941 tcttttcctg attcatgatg gaagcggctt ggccaactac tacggtcgga tgtcaagtgt
     6001 tcagcgcaat gtttggggca ttttcaatcc caattttgcg acatcggaac cttggacaga
     6061 cttgaagacg atggctgcca agtatgcgtc tataatttcc agcacggcac gcgggccagc
     6121 tatcatcgga ggttggtctt tcggtggtgt ggtagctttc gaagcggctc gccaactgat
     6181 gtcggccggc cattctgtca aaggggtcgt cctcatcgat tcgcccagtc cagtcaatca
     6241 cgtacctctc tcggacgctg tgatcgactc ggttgtgaat ttagacagtc gtgcagccaa
     6301 gtcagacatc agtcgattag tcaaacgaca attccagatg aattccgcaa tgttagggaa
     6361 gtatgaacca tcgacaggtg gccattatcc ccctctcgtt ctcctcagat cttcggaggg
     6421 ctaccaacgc cccaacgtgt ctgttccgat ttggttgagc gaccgaagcg acccccgaca
     6481 agccgtcagt ggatggcaac aggttgttgg tactgaggtt agagtgatgg atattacagg
     6541 aaaccacttc gagccattta gccctgcaca tatcccctcg gtttctcaaa gagtttcaga
     6601 agcatgtagt tatcttgaat ctctctaagg agattgataa gacttttcta gttgcatatc
     6661 ttttatattt aaatcttatc tattagttaa ttttttgtaa tttatcctta tatatagtct
     6721 ggttattcta aaatatcatt tcagtatcta aaaattcccc tcttttttca gttatatctt
     6781 aacaggcgac agtccaaatg ttgatttatc ccagtccgat tcatcagggt tgtgaagcat
     6841 tttgtcaatg gtcgaaatca catcagtaat agtgcctctt acttgcctca tagaatttct
     6901 ttctcttaac gtcaccgttt ggtcttttat agtttcgaaa tctatggtga taccaaatgg
     6961 tgttcccaat tcatcgttac gggcgtattt tttaccaatt gaagtattgg aatcgtcaat
     7021 tttaaagtat atctctcttt tacgtaaagc ctgcgagatc ctcttaagta tagcggggaa
     7081 gccatcgtta ttcgatattg tcgtaacaaa tactttgatc ggcgctatgc ggccgccacc
     7141 gcggtggagc tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgagact
     7201 caaacaagat atattttgcc cgccttacag acgaaactag ttggaggtta tggagcatac
     7261 tatcacgtgg gcggccactg gtgagttact acaccccagg ggcaacgttg atgctcctaa
     7321 aaaactctgg ctggacgcaa gccgtaacac ccgtgtcact tcataatcgt ttgcaattca
     7381 gggcttgatc tacacattac cctgttatcc ctattaatta atacggttat ccacagaatc
     7441 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     7501 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     7561 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     7621 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     7681 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     7741 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     7801 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     7861 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     7921 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     7981 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     8041 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     8101 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     8161 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     8221 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     8281 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     8341 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     8401 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     8461 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     8521 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     8581 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     8641 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     8701 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     8761 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     8821 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     8881 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     8941 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     9001 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     9061 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     9121 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     9181 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     9241 tccgcgcaca tttccccgaa aagtgccacc tgaacgaagc atctgtgctt cattttgtag
     9301 aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc tgcattttta
     9361 cagaacagaa atgcaacgcg aaagcgctat tttaccaacg aagaatctgt gcttcatttt
     9421 tgtaaaacaa aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat
     9481 ttttacagaa cagaaatgca acgcgagagc gctattttac caacaaagaa tctatacttc
     9541 ttttttgttc tacaaaaatg catcccgaga gcgctatttt tctaacaaag catcttagat
     9601 tacttttttt ctcctttgtg cgctctataa tgcagtctct tgataacttt ttgcactgta
     9661 ggtccgttaa ggttagaaga aggctacttt ggtgtctatt ttctcttcca taaaaaaagc
     9721 ctgactccac ttcccgcgtt tactgattac tagcgaagct gcgggtgcat tttttcaaga
     9781 taaaggcatc cccgattata ttctataccg atgtggattg cgcatacttt gtgaacagaa
     9841 agtgatagcg ttgatgattc ttcattggtc agaaaattat gaacggtttc ttctattttg
     9901 tctctatata ctacgtatag gaaatgttta cattttcgta ttgttttcga ttcactctat
     9961 gaatagttct tactacaatt tttttgtcta aagagtaata ctagagataa acataaaaaa
    10021 tgtagaggtc gagtttagat gcaagttcaa ggagcgaaag gtggatgggt aggttatata
    10081 gggatatagc acagagatat atagcaaaga gatacttttg agcaatgttt gtggaagcgg
    10141 tattcgcaat attttagtag ctcgttacag tccggtgcgt ttttggtttt ttgaaagtgc
    10201 gtcttcagag cgcttttggt tttcaaaagc gctctgaagt tcctatactt tctagagaat
    10261 aggaacttcg gaataggaac ttcaaagcgt ttccgaaaac gagcgcttcc gaaaatgcaa
    10321 cgcgagctgc gcacatacag ctcactgttc acgtcgcacc tatatctgcg tgttgcctgt
    10381 atatatatat acatgagaag aacggcatag tgcgtgttta tgcttaaatg cgtacttata
    10441 tgcgtctatt tatgtaggat gaaaggtagt ctagtacctc ctgtgatatt atcccattcc
    10501 atgcggggta tcgtatgctt ccttcagcac taccctttag ctgttctata tgctgccact
    10561 cctcaattgg attagtctca tccttcaatg ctatcatttc ctttgatatt ggatcatact
    10621 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
    10681 gtctcgcgcg tttcggtgat gacggtgagc gcgtttcggt gatgacggtg aaaacctcgg
    10741 cgcgccatta ccctgttatc cctaatatac aacgcaaaga agctggaaaa ttggcgaggg
    10801 aatcctgttt ctgtctatcc aagaatgggc atgaggtggc aaccgtcgtg ctagcgtaca
    10861 gggtgcactt tgtaaccatt tgggacaccg gacactcgct gttttcgaaa ttacccttta
    10921 agcgcgggta ttgaaccagg cttatgccca agatcgtagc aagcatttaa ctgtgggaat
    10981 actcaggtat cgtaagatgc aagagttcga atctcttagc aaccattatt tttttcctca
    11041 acataacgag aacacacagg ggcgctatcg cacagaatca aattcgatga ctggaaattt
    11101 tttgttaatt tcagaggtcg cctgacgcat ataccttttt caactgaaaa attgggagaa
    11161 aaaggaaagg tgagagcgcc ggaaccggct tttcatatag aatagagaag cgttcatgac
    11221 taaatgcttg catcacaata cttgaagttg acaatattat ttaaggacct attgtttttt
    11281 ccaataggtg gttagcaatc gtcttacttt ctaacttttc ttacctttta catttcagca
    11341 atatatatat atatatttca aggatatacc attctaatgt ctgcccctaa gaagatcgtc
    11401 gttttgccag gtgaccacgt tggtcaagaa atcacagccg aagccattaa ggttcttaaa
    11461 gctatttctg atgttcgttc caatgtcaag ttcgatttcg aaaatcattt aattggtggt
    11521 gctgctatcg atgctacagg tgttccactt ccagatgagg cgctggaagc ctccaagaag
    11581 gctgatgccg ttttgttagg tgctgtgggt ggtcctaaat ggggtaccgg tagtgttaga
    11641 cctgaacaag gtttactaaa aatccgtaaa gaacttcaat tgtacgccaa cttaagacca
    11701 tgtaactttg catccgactc tcttttagac ttatctccaa tcaagccaca atttgctaaa
    11761 ggtactgact tcgttgttgt cagagaatta gtgggaggta tttactttgg taagagaaag
    11821 gaagacgatg gtgatggtgt cgcttgggat agtgaacaat acaccgttcc agaagtgcaa
    11881 agaatcacaa gaatggccgc tttcatggcc ctacaacatg agccaccatt gcctatttgg
    11941 tccttggata aagctaatgt tttggcctct tcaagattat ggagaaaaac tgtggaggaa
    12001 accatcaaga acgaattccc tacattgaag gttcaacatc aattgattga ttctgccgcc
    12061 atgatcctag ttaagaaccc aacccaccta aatggtatta taatcaccag caacatgttt
    12121 ggtgatatca tctccgatga agcctccgtt atcccaggtt ccttgggttt gttgccatct
    12181 gcgtccttgg cctctttgcc agacaagaac accgcatttg gtttgtacga accatgccac
    12241 ggttctgctc cagatttgcc aaagaataag gtcaacccta tcgccactat cttgtctgct
    12301 gcaatgatgt tgaaattgtc attgaacttg cctgaagaag gtaaggccat tgaagatgca
    12361 gttaaaaagg ttttggatgc aggtatcaga actggtgatt taggtggttc caacagtacc
    12421 accgaagtcg gtgatgctgt cgccgaagaa gttaagaaaa tccttgctta aaaagattct
    12481 ctttttttat gatatttgta cataaacttt ataaatgaaa ttcataatag aaacgacacg
    12541 aaattacaaa atggaatatg ttcatagggt agacgaaact atatacgcaa tctacataca
    12601 tttatcaaga aggagaaaaa ggaggatgta aaggaataca ggtaagcaaa ttgatactaa
    12661 tggctcaacg aaatgacggt atcgataagc ttgatatcga attcctgcag cccgggggat
    12721 ccactagttc tagattaatt aatatctaaa aattgcctta tgatccgtct ctccggttac
    12781 agcctgtgta actgattaat cctgcctttc taatcaccat tctaatgttt taattaaggg
    12841 attttgtctt cattaacggc tttcgctcat aaaaatgtta tgacgttttg cccgcaggcg
    12901 ggaaaccatc cacttcacga gactgatctc ctctgccgga acaccgggca tctccaactt
    12961 ataagttgga gaaataagag aatttcagat tgagagaatg aaaaaaaaaa aaaaaaaaag
    13021 gcagaggaga gcatagaaat ggggttcact ttttggtaaa gctatagcat gcctatcaca
    13081 tataaataga gtgccagtag cgactttttt cacactcgaa atactcttac tactgctctc
    13141 ttgttgtttt tatcacttct tgtttcttct tggtaaatag aatatcaagc tacaaaaagc
    13201 atacaatcaa ctatcaacta ttaactatat cgtaatacac aatggattat aa
//
