LOCUS       sequence-212420-       13375 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13375)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 13375)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHKU42.1-2.2
FEATURES             Location/Qualifiers
     source          1..13375
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(7272..7292)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7272..7290)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(7629..8217)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7709..7728)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8388..9179,9180..9248))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9011..9030
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9249..9353)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9380..10722
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(10343..10390)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     10763..10781
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11076..11483
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             11484..12578
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(12825..12841)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12825..12841)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             13352..13375
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 actagtatgc gcaccgaaac tgcctgccca atcgcaatcg ttggaatagc ggctgagttg
       61 ccgagcggcg aatttgctgc aaacaacctc gactacaacg cgttcttcca attcctcctc
      121 cagcgaggag agtcatacga attcatccca aaggaccgct tcgacataga ctcctggagg
      181 ggaaagcagc tgggacgcat cattgcggaa cgcggatcct tcctgaagaa tcttgattcg
      241 ttcgactacc tcgaattcgg cataacgagc aaggatgctc gtgctatggc cgtcagcacc
      301 cggaagctca ttgagctgtc cttcttagca ctcctggact ccggcataga gtaccgaggc
      361 aggaacgtcg gctgttacgc ctccggtaat gcattcgata tcctgagcgt cggtaccgcg
      421 gatgaattcg aagcgcgagg atccttcgcc ggaatacctg ctcagatcgc gaatcggatt
      481 tcataccacc tggacctgtt aggtccttcg gtgccgacag ataccgcatg cagttcatct
      541 ttgaccgcga tgcatcttgc catccaagca cttcgcacgg atgactgcga agctgcagtc
      601 gtgggcggag cccagatcaa ccagagattg tgggacttcg tccagtatac acagggcggt
      661 gtactcgctc ccgacggcaa gtgtaagccg ttcgacatgg gagcagatgg gttctctcgt
      721 ggcgaaggtg ctgtagtaat ggtcatcaaa ccgctggagg cagcgcttcg cgacggtgac
      781 aacatatatg gcacgatctt gggcaccggc attaacagtg caggatctga ggctccggtg
      841 aacgcaccgg cagcatcagc tcaagaagac actatgcgtc gtgcgttcaa gcaagcgaat
      901 cgcaaggtgg aagatctcga ttacgtcgag ctgcacgcga ccgggactgc taagggagat
      961 cccacggagg cgaattgggt gggagccgcc ttcagacgag aagacgaact tctcgttggg
     1021 agtgtgaaag gcaatatagg ccatctcgag ataaccgctt tccttgcgtc ggtctgcaag
     1081 gtctgcggta tatttaactc cggaatcatt cctcccaacg tcagcctaac ccgcctaaac
     1141 ccggccataa aatgggacga ataccgtatg cgggtgcctc tcgagccaac accgctgtcg
     1201 tgccgctctt ccagcggtaa gtcccttgtt tcgatcaaca gcttcggcat aggaggctcg
     1261 aatggacact gcgttatgga atctcctccg gcttcactac ccaagccggt ttcgctggct
     1321 gagggcgcgc ctgttctctt gctcgccggt ggattatctc cgcgtgcaac tactgcagtc
     1381 tccgattctc tcagcgagct cgtccgggaa cggatctctc agatacccaa catctccact
     1441 ctctacgggc gacgtagccg ccagatgaca tggcgaagtt ttgctgttgt gtcgcccagc
     1501 gacatgacta aaatcaggtt ttcgcaaccc actctgacgc cgcgagcccg tccagctgtc
     1561 ggttttgtat tttccggcca aggtccccaa catatgcaca tgggtcgtca gctgtacaaa
     1621 gaattcccgg cattccgcga aagcgtcacg actttggacg gggtgtacag ccaggtggtc
     1681 ggccattctc tcgtggagaa agtgggcatg ttcaaagaca tcggcgccgc atcctcctcc
     1741 atgcctgacg tctggcctat tgatatcatt ctccccgcga tcgccatggt tcagatcgca
     1801 ttgttcgacc tcctcgccag cgtcggagta cggcctgatt ttgtcttggg ccattccgcc
     1861 ggagagacta cgatgttgta cgcgtccggt gctgcccctc gggctatggc tctggagctc
     1921 gcgatcgcgc gaggacaagt cctggccctc actgaaagcg ccggcggcac catgaccgcc
     1981 ttgtcctgcg ggcctgaagt agcgaagcct ctactcgacg aggtgaaggc gacgcgtgac
     2041 gcaggcatcc tcgaaatcgc atgtcacaac agcgcggagg ccatcacgat tgccggccac
     2101 gattatctcg ttgacgcagc tgtggaactc tgcaagtcga aaggaattgc cgcgaccaga
     2161 ctgcgtacat ccgtggccgt gcacagctca atgatggagc tttgcaagga cgagtactac
     2221 gaacgtgtcc tgcaagtctt tgcgaaatat cccgaggcgc gcttaacgcc aaatgtggac
     2281 acattctcca ccgtaacggg cgaaaggctg gcggagccgt tctcaccaga ctactactgg
     2341 aaaaacacta gggaaccggt gctattcatg gagacagttc gctctgctca agccgcgata
     2401 tccgggcgta atctattagt agtcgaagtg agcccgcatc cagttctctc cgcgtacctt
     2461 tccgcgcttt gtccggaatc tgcaactatc gtcggtccga tgcgacgaac gaagcagatg
     2521 ttcccacatt ttgagcgttc taccttcttg caagcggtcg ggaagctcat cgcgggcggt
     2581 tacaactcaa ccgacttccc cgccctcaac ggcggagcgt cgcagcacgt ggagtttggt
     2641 gtgcctgcat atccctttgc accgaagagc gtgcctcata tggcgaaatt accggagaac
     2701 gacgagatct atgggtcccg actaggaccc ttgaaccatt ctcgacttcg cctcaatgcg
     2761 gcgactcatc cagatctcgc gcaacacgtc atgaaagggg aaccgattgt tcctgctaca
     2821 ggtttcgtgg aaatggctct ggagttcggt gcgagatctt tatgggacgt gaagttcctc
     2881 tccttcctat ctctttcggc ggaggaacct gtccgcgtcc gcttcgagcc cgacggcttc
     2941 aagtggtcga tcaagacaac ttcatatagc tcacatggtt ctggcgcggc cagcaagaag
     3001 gccgagcgat tacatgctgg cggatacttc tcgacagagc caacagccgc caacccccct
     3061 tctcctcgtc tagacgagat tcgggagagg tgtcattgga tcaacgtctc aggcttctat
     3121 gacgacctcc gctatttcgc tgaatacggc ccatggttcc agatgatcac gtccgttcat
     3181 cgcggcgagg aagaattttt ggtcgaagtt gatacacggc ggcagggcgt caatgttccg
     3241 acatcgcatt atgtcttcga tccggccatc ctcgacgcat gcattcacgt tatggtccat
     3301 cccatgatca ctggcaacac tgatcgcaac gtatactact taccatcacg tttcgatttt
     3361 ttctcgacat atgacgcctt gcatcgtcgc gaatttacgg acaagttgta tgcgcatggg
     3421 tctcgaggaa gatggacccc ggagggcata accttcgacg tcctggtagt caatagtcaa
     3481 ggaacgccgt tgtgctcctt ccgaggcttt gaggtcgcct accacggttt agcgccctcg
     3541 gtgggaacgc gctatgatct cgaatacacc actgtggcga gcctggacct gaatgcgcgc
     3601 gttcagaagg cggactcgtt ctcgtgcgac gagattacta aggtcacaga tcgcaccctc
     3661 tacttccgct acatacgagg caaggaggat ttactgcgta gtgagctcga gaaggtcgac
     3721 ctgactgggt tgtccatttg gttgcttgcg ctctccggcg tcgacggcga cgctgccctt
     3781 gggttttcgc gttccttacg acgcgagcta ctagcgaacg aggtgcgcct ggtgatattc
     3841 gaggctcgca tatcattccc ggcacaagtc gaggtgatat cggggttgtt acaagagaac
     3901 gtcgccgagg atgaaatcac tgtggactcc actggtcggg ttcttgttcc taggcttcaa
     3961 gcgtcgtcac ctccagtaga atcgacttct ctgaatcccg acgcaccttg gtctgtgaag
     4021 cgtactggat cgggagattc gaaagcagtt atcgttcaaa catcgccgcc cattcccgaa
     4081 cagcatgaag tcctggtgaa ggtcgattac gtcgatacgc tccagcaatc gggcatttgg
     4141 gccttcgtag ggcgtaccga ttccggcact gctgtcgctg gactcgcctc tgatcatctg
     4201 gcaagccatg ttgtcgtgca tcgcgcagtc gtcaccgagc tgtcgaacat cgggttcgac
     4261 accgcggcgc gtcttcctgg ttacattgca gctctctcga tcggtttcct cgctttggga
     4321 ttcaaagcgg aggttcccag cctcctcgcg cagaacacag cgctggtcac ccatgccgac
     4381 acgctgctag gtaccctcct tgtggagctt ctcaaggaca gaggactgca tgtcacggct
     4441 gtgaacgaaa ccgacggcct tagtaccatc tgtggtctgc aatcgccaaa gtctttcgac
     4501 cacgttttga gtggattcca cgatcccgcc tcgctcagcg tccttcacag tatctgttcc
     4561 acagaaaatg cagtcttcag ctggaaccat cccgatcgcg gcattgctgc cattttaaag
     4621 aatgatccct acagaatcgg ttacgcgctt cgttccggac ttaagtctct tccatcttca
     4681 tacaagaaga tggagtacag catgcccgtt caccgccttc tccctttgac ctccgacgtg
     4741 accatcctgg ataacactgc gatattcgac cccgcgaaga catatatatt gttcggcggc
     4801 ataggcagct tggggttgcg cattgcgact tggatgtatg agaacggcgc acgccatctt
     4861 gtcctgactt cccgttccgg tcgtgaaact ctcacaaaga ccaagaactt cgccgccatg
     4921 cgtgttctgc gctatttgac ggatttaact gacctcgaca tcaagcttct cgcttccgac
     4981 gcgacgaacg tcacagatct ccgcagagtg ctatccgcga taagttgtcc catcggcgga
     5041 tgcatgatgc taacagctgt cctcgaggac aagatgttcg cttcgttaac tcaagacagc
     5101 tttgaaaggg tcttcgccgc gaagatagga gccttcaacg ccctcactca ggcgctcgat
     5161 atcaccagtc tcgatttcct gatcactttc acgtccatat caggcatgtt tggcagcgca
     5221 ggacagacca actatgctgc agcgaacacc gcgctggagg ctttgactcg gccttaccca
     5281 aatgcattct ccttggtggc acccgcgatg ctcgacacgg ctattgtctt cgccgacgag
     5341 caaagcaaga ctactcataa caccaaagtc cgccatatta gtgaatgggg catgtcgtcg
     5401 cgagagcttt gcgcatgcat cgaagatggc atccgcaaac tgcggaacgg gccattctgg
     5461 ttatacgttc ccgatttcaa gtgggcggct gtccgcgata atcttggcgt ctctcccgtc
     5521 ttcgcacatc tcgttccgga ggttgatgaa gacacgtcaa cagcggacgc agcctccaaa
     5581 gactctagtg tcttagatat tgtcctcaag gtcctggact tggataagga tgacttttcc
     5641 tttgaggttc cactcaccgc atacggactc gactcgttat tggcgtcgaa gcttgcggta
     5701 acgcttcgcc cttgggtttc aataacccaa ctgcagctgc tggcagatat cacgttcgag
     5761 gacatacaac gacggataga ggcgcaggcc ggacaggaag tcgaacaacc ggccgacaag
     5821 gacttctttg actgggagga agtcatgcgt tccggccccg cttgcgtcaa gctcaccgat
     5881 ggggaaggcc tgccgctgtt cgtcctccat ggccctgccg gcactatcga agccttcggc
     5941 cacatgcgac tcaatttcaa gcatactccg ctctacggag tgcaagccac gaaggacgcg
     6001 ccttatgaca acctcgaccg ccttgccgca ttctatcatt cgaacatcaa aagcgtacaa
     6061 ccgcaagggc cgtaccgttt gggcggcttc tgcggctcta gtttactact tttcatcctc
     6121 gcggatatct tccgcaaaaa cggcgacatc gtcgtgcaaa tgtccatgat cgatcatttc
     6181 cctctactat acacctcgcc catctgggag ctggacgccg ccacggccaa agatcgctgc
     6241 gttagtgccg ccctgaagtc ccgcggtttc gagtggatca tgaacttgta tcgggtcgag
     6301 aaaaatccca acgaaaagcg cgccggggag gagctgatga aggctgggct gggcgagaag
     6361 gttcagccgc acgtcaagaa gctgtacgaa aacacgatgg tgttcatcga agctcacgca
     6421 tcgtggctcg cttcgcactt gctcaaggag ggcgaggagg ttgacggtac gatactgcgt
     6481 gaaagactca tcgcctacct gtcaacattc gactttcccg cgacggtgta cgctgccaaa
     6541 gacggtatgc agaagatatt cacggacgag cagcaggcgc aatgggccca tatgggcgca
     6601 acgatgtgca ccaatcccat tcgtgtcgcg gaaatcgagg cgtcgcactt cgatatcctc
     6661 agcaacctgg acttcataca tagactcgat accgaagtaa ctctcactgc atctaatgta
     6721 atgaaggcta tgtagggaga ttgataagac ttttctagtt gcatatcttt tatatttaaa
     6781 tcttatctat tagttaattt tttgtaattt atccttatat atagtctggt tattctaaaa
     6841 tatcatttca gtatctaaaa attcccctct tttttcagtt atatcttaac aggcgacagt
     6901 ccaaatgttg atttatccca gtccgattca tcagggttgt gaagcatttt gtcaatggtc
     6961 gaaatcacat cagtaatagt gcctcttact tgcctcatag aatttctttc tcttaacgtc
     7021 accgtttggt cttttatagt ttcgaaatct atggtgatac caaatggtgt tcccaattca
     7081 tcgttacggg cgtatttttt accaattgaa gtattggaat cgtcaatttt aaagtatatc
     7141 tctcttttac gtaaagcctg cgagatcctc ttaagtatag cggggaagcc atcgttattc
     7201 gatattgtcg taacaaatac tttgatcggc gctatgcggc cgccaccgcg gtggagctcc
     7261 agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgagactcaa acaagatata
     7321 ttttgcccgc cttacagacg aaactagttg gaggttatgg agcatactat cacgtgggcg
     7381 gccactggtg agttactaca ccccaggggc aacgttgatg ctcctaaaaa actctggctg
     7441 gacgcaagcc gtaacacccg tgtcacttca taatcgtttg caattcaggg cttgatctac
     7501 acattaccct gttatcccta ttaattaata cggttatcca cagaatcagg ggataacgca
     7561 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     7621 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     7681 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     7741 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     7801 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     7861 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     7921 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     7981 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     8041 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     8101 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     8161 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     8221 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     8281 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     8341 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     8401 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     8461 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     8521 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     8581 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     8641 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     8701 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     8761 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     8821 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     8881 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     8941 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     9001 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     9061 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     9121 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     9181 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     9241 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     9301 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     9361 ccccgaaaag tgccacctga acgaagcatc tgtgcttcat tttgtagaac aaaaatgcaa
     9421 cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc atttttacag aacagaaatg
     9481 caacgcgaaa gcgctatttt accaacgaag aatctgtgct tcatttttgt aaaacaaaaa
     9541 tgcaacgcga gagcgctaat ttttcaaaca aagaatctga gctgcatttt tacagaacag
     9601 aaatgcaacg cgagagcgct attttaccaa caaagaatct atacttcttt tttgttctac
     9661 aaaaatgcat cccgagagcg ctatttttct aacaaagcat cttagattac tttttttctc
     9721 ctttgtgcgc tctataatgc agtctcttga taactttttg cactgtaggt ccgttaaggt
     9781 tagaagaagg ctactttggt gtctattttc tcttccataa aaaaagcctg actccacttc
     9841 ccgcgtttac tgattactag cgaagctgcg ggtgcatttt ttcaagataa aggcatcccc
     9901 gattatattc tataccgatg tggattgcgc atactttgtg aacagaaagt gatagcgttg
     9961 atgattcttc attggtcaga aaattatgaa cggtttcttc tattttgtct ctatatacta
    10021 cgtataggaa atgtttacat tttcgtattg ttttcgattc actctatgaa tagttcttac
    10081 tacaattttt ttgtctaaag agtaatacta gagataaaca taaaaaatgt agaggtcgag
    10141 tttagatgca agttcaagga gcgaaaggtg gatgggtagg ttatataggg atatagcaca
    10201 gagatatata gcaaagagat acttttgagc aatgtttgtg gaagcggtat tcgcaatatt
    10261 ttagtagctc gttacagtcc ggtgcgtttt tggttttttg aaagtgcgtc ttcagagcgc
    10321 ttttggtttt caaaagcgct ctgaagttcc tatactttct agagaatagg aacttcggaa
    10381 taggaacttc aaagcgtttc cgaaaacgag cgcttccgaa aatgcaacgc gagctgcgca
    10441 catacagctc actgttcacg tcgcacctat atctgcgtgt tgcctgtata tatatataca
    10501 tgagaagaac ggcatagtgc gtgtttatgc ttaaatgcgt acttatatgc gtctatttat
    10561 gtaggatgaa aggtagtcta gtacctcctg tgatattatc ccattccatg cggggtatcg
    10621 tatgcttcct tcagcactac cctttagctg ttctatatgc tgccactcct caattggatt
    10681 agtctcatcc ttcaatgcta tcatttcctt tgatattgga tcatactaag aaaccattat
    10741 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt
    10801 cggtgatgac ggtgagcgcg tttcggtgat gacggtgaaa acctcggcgc gccattaccc
    10861 tgttatccct aatatacaac gcaaagaagc tggaaaattg gcgagggaat cctgtttctg
    10921 tctatccaag aatgggcatg aggtggcaac cgtcgtgcta gcgtacaggg tgcactttgt
    10981 aaccatttgg gacaccggac actcgctgtt ttcgaaatta ccctttaagc gcgggtattg
    11041 aaccaggctt atgcccaaga tcgtagcaag catttaactg tgggaatact caggtatcgt
    11101 aagatgcaag agttcgaatc tcttagcaac cattattttt ttcctcaaca taacgagaac
    11161 acacaggggc gctatcgcac agaatcaaat tcgatgactg gaaatttttt gttaatttca
    11221 gaggtcgcct gacgcatata cctttttcaa ctgaaaaatt gggagaaaaa ggaaaggtga
    11281 gagcgccgga accggctttt catatagaat agagaagcgt tcatgactaa atgcttgcat
    11341 cacaatactt gaagttgaca atattattta aggacctatt gttttttcca ataggtggtt
    11401 agcaatcgtc ttactttcta acttttctta ccttttacat ttcagcaata tatatatata
    11461 tatttcaagg atataccatt ctaatgtctg cccctaagaa gatcgtcgtt ttgccaggtg
    11521 accacgttgg tcaagaaatc acagccgaag ccattaaggt tcttaaagct atttctgatg
    11581 ttcgttccaa tgtcaagttc gatttcgaaa atcatttaat tggtggtgct gctatcgatg
    11641 ctacaggtgt tccacttcca gatgaggcgc tggaagcctc caagaaggct gatgccgttt
    11701 tgttaggtgc tgtgggtggt cctaaatggg gtaccggtag tgttagacct gaacaaggtt
    11761 tactaaaaat ccgtaaagaa cttcaattgt acgccaactt aagaccatgt aactttgcat
    11821 ccgactctct tttagactta tctccaatca agccacaatt tgctaaaggt actgacttcg
    11881 ttgttgtcag agaattagtg ggaggtattt actttggtaa gagaaaggaa gacgatggtg
    11941 atggtgtcgc ttgggatagt gaacaataca ccgttccaga agtgcaaaga atcacaagaa
    12001 tggccgcttt catggcccta caacatgagc caccattgcc tatttggtcc ttggataaag
    12061 ctaatgtttt ggcctcttca agattatgga gaaaaactgt ggaggaaacc atcaagaacg
    12121 aattccctac attgaaggtt caacatcaat tgattgattc tgccgccatg atcctagtta
    12181 agaacccaac ccacctaaat ggtattataa tcaccagcaa catgtttggt gatatcatct
    12241 ccgatgaagc ctccgttatc ccaggttcct tgggtttgtt gccatctgcg tccttggcct
    12301 ctttgccaga caagaacacc gcatttggtt tgtacgaacc atgccacggt tctgctccag
    12361 atttgccaaa gaataaggtc aaccctatcg ccactatctt gtctgctgca atgatgttga
    12421 aattgtcatt gaacttgcct gaagaaggta aggccattga agatgcagtt aaaaaggttt
    12481 tggatgcagg tatcagaact ggtgatttag gtggttccaa cagtaccacc gaagtcggtg
    12541 atgctgtcgc cgaagaagtt aagaaaatcc ttgcttaaaa agattctctt tttttatgat
    12601 atttgtacat aaactttata aatgaaattc ataatagaaa cgacacgaaa ttacaaaatg
    12661 gaatatgttc atagggtaga cgaaactata tacgcaatct acatacattt atcaagaagg
    12721 agaaaaagga ggatgtaaag gaatacaggt aagcaaattg atactaatgg ctcaacgaaa
    12781 tgacggtatc gataagcttg atatcgaatt cctgcagccc gggggatcca ctagttctag
    12841 attaattaat atctaaaaat tgccttatga tccgtctctc cggttacagc ctgtgtaact
    12901 gattaatcct gcctttctaa tcaccattct aatgttttaa ttaagggatt ttgtcttcat
    12961 taacggcttt cgctcataaa aatgttatga cgttttgccc gcaggcggga aaccatccac
    13021 ttcacgagac tgatctcctc tgccggaaca ccgggcatct ccaacttata agttggagaa
    13081 ataagagaat ttcagattga gagaatgaaa aaaaaaaaaa aaaaaaggca gaggagagca
    13141 tagaaatggg gttcactttt tggtaaagct atagcatgcc tatcacatat aaatagagtg
    13201 ccagtagcga cttttttcac actcgaaata ctcttactac tgctctcttg ttgtttttat
    13261 cacttcttgt ttcttcttgg taaatagaat atcaagctac aaaaagcata caatcaacta
    13321 tcaactatta actatatcgt aatacacaat ggattataag gatgatgatg ataag
//
