LOCUS       sequence-212435-         371 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Arg49 of TBP6.7 and other TBPs is the only arginine that was 
            lab-evolved from wild-type U1A to play a significant role in TAR RNA
            recognition. Mutation results in ~230-fold reduction in Kd..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Belashov IA, Crawford DW, Cavender CE, Dai P, Beardslee PC, Mathews 
            DH, Pentelute BL, McNaughton BR, Wedekind JE
  TITLE     Structure of HIV TAR in complex with a Lab-Evolved RRM provides 
            insight into duplex RNA recognition and synthesis of a constrained 
            peptide that impairs transcription.
  JOURNAL   Nucleic Acids Res. 2018 Jul 27;46(13):6401-6415. doi: 
            10.1093/nar/gky529.
  PUBMED    29961805
REFERENCE   2  (bases 1 to 371)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2018-07-27- 27"; volume: "46"; issue: 
            "13"; pages: "6401-6415"
COMMENT     Sequence Label: pET28a_6HT-TBP6.7(R49A)
FEATURES             Location/Qualifiers
     source          1..371
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             15..32
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             42..62
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ccatgggcag cagccatcat catcatcatc acagcagcgg cgagaacctg tacttccagg
       61 gccatatggc agttcccgag acccgcccta accacactat ttatatcaac aacctcaatt
      121 cgaagatcaa gaaggatgag ctaaaaaagt ccctgcacgc catcttctcc cggtttggcc
      181 agatcctgga tatcctggta ccgcggcagg cgacgccgag gggccaggcc tttgtcatct
      241 tcaaggaggt cagcagcgcc accaacgccc tgcgctccat gcagggtttc cctttctatg
      301 acaaacctat gcgtatccag tatgccaaga ccgacaagcg tatcccggcc aagatgaaat
      361 gatagggatc c
//
