LOCUS       sequence-212455-        6656 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The 1.3xHBV-Luciferase plasmid to monitor HBV transcription. 1. 
            Luciferase inserted within Core ORF. 2. Luciferase is regulated by 
            EnhII - Core promoter. 3. The functional luciferase ATG is of Core.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6656)
  AUTHORS   Doitsh G, Shaul Y
  TITLE     Enhancer I predominance in hepatitis B virus gene expression.
  JOURNAL   Mol Cell Biol. 2004 Feb;24(4):1799-808.
  PUBMED    14749394
REFERENCE   2  (bases 1 to 6656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2004-02"; volume: "24(4)"; pages: "1799-808"
COMMENT     Sequence Label: 1.3xWT HBV-Luciferase
FEATURES             Location/Qualifiers
     source          1..6656
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             972..2621
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="EDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAH
                     IEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPA
                     NDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSM
                     YTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHAR
                     DPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKI
                     QSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYG
                     LTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSG
                     YVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILL
                     QHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVF
                     VDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(1035..1053)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     promoter        complement(3981..3999)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3982..3999)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4017..4033)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4017..4033)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4030..4052)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4041..4057
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4065..4071,4072..4089,4090..4095))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4110..4131
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4248..4265)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4419..5007)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4499..4518)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5178..5969,5970..6038))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5801..5820
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6039..6143)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6211..6229
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6267..6289)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6389..6408
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6602..6624
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6616..6633
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6617..6633
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(6640..6656,1..3)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(6640..6656,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattc gagctcggta cccatcctgc cttaatgcct ttgtatgcat gtatacaagc
       61 taaacaggct ttcactttct cgccaactta caaggccttt ctaagtaaac agtacatgaa
      121 cctttacccc gttgctcggc aacggcctgg tctgtgccaa gtgtttgctg acgcaacccc
      181 cactggctgg ggcttggcca taggccatca gcgcatgcgt ggaacctttg tggctcctct
      241 gccgatccat actgcggaac tcctagccgc ttgttttgct cgcagccggt ctggagcaaa
      301 gctcatcgga actgacaatt ctgtcgtcct ctcgcggaaa tatacatcgt ttccatggct
      361 gctaggctgt actgccaact ggatccttcg cgggacgtcc tttgtttacg tcccgtcggc
      421 gctgaatccc gcggacgacc cctcgcgggg ccgcttggga ctctctcgtc cccttctccg
      481 tctgccgttc cagccgacca cggggcgcac ctctctttac gcggtctccc cgtctgtgcc
      541 ttctcatctg ccggtccgtg tgcacttcgc ttcacctctg cacgttgcat ggagaccacc
      601 gtgaacgccc atcagatcct gcccaaggtc ttacataaga ggactcttgg actcccagca
      661 atgtcaacga ccgaccttga ggcctacttc aaagactgtg tgtttaagga ctgggaggag
      721 ctgggggagg agattaggtt aaaggtcttt gtattaggag gctgtaggca taaattggtc
      781 tgcgcaccag caccatgcaa ctttttcacc tctgcctaat catctcttgt acatgtccca
      841 ctgttcaagc ctccaagctg tgccttgggt ggctttgggg catggacatt gacccttata
      901 aagaatttgg agctactgtg gagttactct cgtttttgcc ttctgacttc tttccttccg
      961 tcagagatct cgaagacgcc aaaaacataa agaaaggccc ggcgccattc tatccgctgg
     1021 aagatggaac cgctggagag caactgcata aggctatgaa gagatacgcc ctggttcctg
     1081 gaacaattgc ttttacagat gcacatatcg aggtggacat cacttacgct gagtacttcg
     1141 aaatgtccgt tcggttggca gaagctatga aacgatatgg gctgaataca aatcacagaa
     1201 tcgtcgtatg cagtgaaaac tctcttcaat tctttatgcc ggtgttgggc gcgttattta
     1261 tcggagttgc agttgcgccc gcgaacgaca tttataatga acgtgaattg ctcaacagta
     1321 tgggcatttc gcagcctacc gtggtgttcg tttccaaaaa ggggttgcaa aaaattttga
     1381 acgtgcaaaa aaagctccca atcatccaaa aaattattat catggattct aaaacggatt
     1441 accagggatt tcagtcgatg tacacgttcg tcacatctca tctacctccc ggttttaatg
     1501 aatacgattt tgtgccagag tccttcgata gggacaagac aattgcactg atcatgaact
     1561 cctctggatc tactggtctg cctaaaggtg tcgctctgcc tcatagaact gcctgcgtga
     1621 gattctcgca tgccagagat cctatttttg gcaatcaaat cattccggat actgcgattt
     1681 taagtgttgt tccattccat cacggttttg gaatgtttac tacactcgga tatttgatat
     1741 gtggatttcg agtcgtctta atgtatagat ttgaagaaga gctgtttctg aggagccttc
     1801 aggattacaa gattcaaagt gcgctgctgg tgccaaccct attctccttc ttcgccaaaa
     1861 gcactctgat tgacaaatac gatttatcta atttacacga aattgcttct ggtggcgctc
     1921 ccctctctaa ggaagtcggg gaagcggttg ccaagaggtt ccatctgcca ggtatcaggc
     1981 aaggatatgg gctcactgag actacatcag ctattctgat tacacccgag ggggatgata
     2041 aaccgggcgc ggtcggtaaa gttgttccat tttttgaagc gaaggttgtg gatctggata
     2101 ccgggaaaac gctgggcgtt aatcaaagag gcgaactgtg tgtgagaggt cctatgatta
     2161 tgtccggtta tgtaaacaat ccggaagcga ccaacgcctt gattgacaag gatggatggc
     2221 tacattctgg agacatagct tactgggacg aagacgaaca cttcttcatc gttgaccgcc
     2281 tgaagtctct gattaagtac aaaggctatc aggtggctcc cgctgaattg gaatccatct
     2341 tgctccaaca ccccaacatc ttcgacgcag gtgtcgcagg tcttcccgac gatgacgccg
     2401 gtgaacttcc cgccgccgtt gttgttttgg agcacggaaa gacgatgacg gaaaaagaga
     2461 tcgtggatta cgtcgccagt caagtaacaa ccgcgaaaaa gttgcgcgga ggagttgtgt
     2521 ttgtggacga agtaccgaaa ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga
     2581 tcctcataaa ggccaagaag ggcggaaaga tcgccgtgta aactagtgcc atttgttcag
     2641 tggttcgtag ggctttcccc cactgtttgg ctttcagcta tatggatgat gtggtattgg
     2701 gggccaagtc tgtacagcat cgtgagtccc tttataccgc tgttaccaat tttcttttgt
     2761 ctctgggtat acatttaaac cctaacaaaa caaaaagatg gggttattcc ctaaacttca
     2821 tgggctacat aattggaagt tggggaactt tgccacagga tcatattgta caaaagatca
     2881 aacactgttt tagaaaactt cctgttaaca ggcctattga ttggaaagta tgtcaaagaa
     2941 ttgtgggtct tttgggcttt gctgctccat ttacacaatg tggatatcct gccttaatgc
     3001 ctttgtatgc atgtatacaa gctaaacagg ctttcacttt ctcgccaact tacaaggcct
     3061 ttctaagtaa acagtacatg aacctttacc ccgttgctcg gcaacggcct ggtctgtgcc
     3121 aagtgtttgc tgacgcaacc cccactggct ggggcttggc cataggccat cagcgcatgc
     3181 gtggaacctt tgtggctcct ctgccgatcc atactgcgga actcctagcc gcttgttttg
     3241 ctcgcagccg gtctggagca aagctcatcg gaactgacaa ttctgtcgtc ctctcgcgga
     3301 aatatacatc gtttccatgg ctgctaggct gtactgccaa ctggatcctt cgcgggacgt
     3361 cctttgttta cgtcccgtcg gcgctgaatc ccgcggacga cccctctcgg ggccgcttgg
     3421 gactctctcg tccccttctc cgtctgccgt tccagccgac cacggggcgc acctctcttt
     3481 acgcggtctc cccgtctgtg ccttctcatc tgccggtccg tgtgcacttc gcttcacctc
     3541 tgcacgttgc atggagacca ccgtgaacgc ccatcagatc ctgcccaagg tcttacataa
     3601 gaggactctt ggactcccag caatgtcaac gaccgacctt gaggcctact tcaaagactg
     3661 tgtgtttaag gactgggagg agctggggga ggagattagg ttaaaggtct ttgtattagg
     3721 aggctgtagg cacaaattgg tctgcgcacc agcaccatgc aactttttca cctctgccta
     3781 atcatctctt gtacatgtcc cactgttcaa gcctccaagc tgtgccttgg gtggctttgg
     3841 ggcatggaca ttgaccctta taaagaattt ggagctactg tggagttact ctcgtttttg
     3901 ccttctgact tctttccttc cgtcagagat ctcctagaca ccgcctcagc tctgtattgc
     3961 aggcatgcaa gcttgagtat tctatagtgt cacctaaata gcttggcgta atcatggtca
     4021 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     4081 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     4141 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     4201 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     4261 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     4321 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     4381 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     4441 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     4501 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     4561 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     4621 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     4681 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     4741 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     4801 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     4861 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     4921 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     4981 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     5041 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     5101 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     5161 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     5221 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     5281 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     5341 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     5401 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     5461 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     5521 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     5581 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     5641 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     5701 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     5761 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     5821 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     5881 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     5941 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     6001 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     6061 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     6121 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     6181 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc
     6241 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     6301 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     6361 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac
     6421 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat
     6481 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     6541 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     6601 tcccagtcac gacgttgtaa aacgacggcc agtgaattgt aatacgactc actata
//
