LOCUS       sequence-212456-       12747 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  human AAVS1 site targeting donor plasmid for knock-in hM3Dq-mCherry 
            expression cassette driven by CAG promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12747)
  AUTHORS   Chen Y, Xiong M, Dong Y, Haberman A, Cao J, Liu H, Zhou W, Zhang SC
  TITLE     Chemical Control of Grafted Human PSC-Derived Neurons in a Mouse 
            Model of Parkinson's Disease.
  JOURNAL   Cell Stem Cell. 2016 Jun 2;18(6):817-26. doi: 
            10.1016/j.stem.2016.03.014. Epub 2016 Apr 28.
  PUBMED    27133795
REFERENCE   2  (bases 1 to 12747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2016.03.014"; journalName: "Cell Stem Cell"; date: 
            "2016-06-2- 2"; volume: "18"; issue: "6"; pages: "817-26"
COMMENT     Sequence Label: AAVS1-Pur-CAG-hM3Dq-mCherry
FEATURES             Location/Qualifiers
     source          1..12747
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    37..840
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    847..872
                     /label=SA
                     /note="splice acceptor site"
     CDS             896..949
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             959..1558
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(959..978)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1455..1475
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(1590..1607)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1596..1820
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        1826..2205
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2207..2484
                     /label=chicken beta-actin promoter
     intron          2486..3503
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3427..3449
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     regulatory      5358..5367
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5364..6074
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5509..5527)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5777..5796
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6108..6696
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6161..6181)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6579..6590)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    6728..7204
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(6927..6946)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(7314..7333)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7379..7434
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7433..7452)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7795..7811)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7819..7835
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7843..7849,7850..7867,7868..7873))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7888..7909
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    7983..8819
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(8853..8872)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8854..8872)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8879..8896)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8879..8895)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8888..8910)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             9033..9335
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     9214..9233
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     primer_bind     complement(9738..9757)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10352..10371
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             complement(join(10732..11523,11524..11592))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11355..11374
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11716..12304
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12205..12224
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12458..12475
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12592..12613
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12628..12633,12634..12651,12652..12658)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12666..12682
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12690..12706
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        12727..12745
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ctagctagtc ctgcaggttt aaacgaattc gccctttgct ttctctgacc agcattctct
       61 cccctgggcc tgtgccgctt tctgtctgca gcttgtggcc tgggtcacct ctacggctgg
      121 cccagatcct tccctgccgc ctccttcagg ttccgtcttc ctccactccc tcttcccctt
      181 gctctctgct gtgttgctgc ccaaggatgc tctttccgga gcacttcctt ctcggcgctg
      241 caccacgtga tgtcctctga gcggatcctc cccgtgtctg ggtcctctcc gggcatctct
      301 cctccctcac ccaaccccat gccgtcttca ctcgctgggt tcccttttcc ttctccttct
      361 ggggcctgtg ccatctctcg tttcttagga tggccttctc cgacggatgt ctcccttgcg
      421 tcccgcctcc ccttcttgta ggcctgcatc atcaccgttt ttctggacaa ccccaaagta
      481 ccccgtctcc ctggctttag ccacctctcc atcctcttgc tttctttgcc tggacacccc
      541 gttctcctgt ggattcgggt cacctctcac tcctttcatt tgggcagctc ccctaccccc
      601 cttacctctc tagtctgtgc tagctcttcc agccccctgt catggcatct tccaggggtc
      661 cgagagctca gctagtcttc ttcctccaac ccgggcccct atgtccactt caggacagca
      721 tgtttgctgc ctccagggat cctgtgtccc cgagctggga ccaccttata ttcccagggc
      781 cggttaatgt ggctctggtt ctgggtactt ttatctgtcc cctccacccc acagtggggc
      841 aagcttctga cctcttctct tcctcccaca gggcctcgag agatctggca gcggagaggg
      901 cagaggaagt cttctaacat gcggtgacgt ggaggagaat cccggcccta ggctcgagat
      961 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     1021 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     1081 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     1141 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     1201 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     1261 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     1321 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     1381 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     1441 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     1501 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgatc
     1561 tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     1621 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     1681 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     1741 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     1801 ggatgcggtg ggctctatgg gtctcgacat tgattattga ctagttatta atagtaatca
     1861 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     1921 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     1981 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     2041 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     2101 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     2161 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtcga ggtgagcccc
     2221 acgttctgct tcactctccc catctccccc ccctccccac ccccaatttt gtatttattt
     2281 attttttaat tattttgtgc agcgatgggg gcgggggggg ggggggggcg cgcgccaggc
     2341 ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat
     2401 cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg gcggccctat
     2461 aaaaagcgaa gcgcgcggcg ggcggggagt cgctgcgacg ctgccttcgc cccgtgcccc
     2521 gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg
     2581 tgagcgggcg ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc
     2641 ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg
     2701 gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct
     2761 ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca
     2821 gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg
     2881 ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt
     2941 cggtcgggct gcaacccccc ctgcaccccc ctccccgagt tgctgagcac ggcccggctt
     3001 cgggtgcggg gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc
     3061 ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc cggggagggc tcgggggagg
     3121 ggcgcggcgg cccccggagc gccggcggct gtcgaggcgc ggcgagccgc agccattgcc
     3181 ttttatggta atcgtgcgag agggcgcagg gacttccttt gtcccaaatc tgtgcggagc
     3241 cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg
     3301 ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc
     3361 tccctctcca gcctcggggc tgtccgcggg gggacggctg ccttcggggg ggacggggca
     3421 gggcggggtt cggcttctgg cgtgtgaccg gcggctctag agcctctgct aaccatgttc
     3481 atgccttctt ctttttccta cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc
     3541 attttggcaa agaattcgtc gacgccgcca ccatgacctt gcacaataac agtacaacct
     3601 cgcctttgtt tccaaacatc agctcctcct ggatacacag cccctccgat gcagggctgc
     3661 ccccgggaac cgtcactcat ttcggcagct acaatgtttc tcgagcagct ggcaatttct
     3721 cctctccaga cggtaccacc gatgaccctc tgggaggtca taccgtctgg caagtggtct
     3781 tcatcgcttt cttaacgggc atcctggcct tggtgaccat catcggcaac atcctggtaa
     3841 ttgtgtcatt taaggtcaac aagcagctga agacggtcaa caactacttc ctcttaagcc
     3901 tggcctgtgc cgatctgatt atcggggtca tttcaatgaa tctgtttacg acctacatca
     3961 tcatgaatcg atgggcctta gggaacttgg cctgtgacct ctggcttgcc attgactgcg
     4021 tagccagcaa tgcctctgtt atgaatcttc tggtcatcag ctttgacaga tacttttcca
     4081 tcacgaggcc gctcacgtac cgagccaaac gaacaacaaa gagagccggt gtgatgatcg
     4141 gtctggcttg ggtcatctcc tttgtccttt gggctcctgc catcttgttc tggcaatact
     4201 ttgttggaaa gagaactgtg cctccgggag agtgcttcat tcagttcctc agtgagccca
     4261 ccattacttt tggcacagcc atcgctggtt tttatatgcc tgtcaccatt atgactattt
     4321 tatactggag gatctataag gaaactgaaa agcgtaccaa agagcttgct ggcctgcaag
     4381 cctctgggac agaggcagag acagaaaact ttgtccaccc cacgggcagt tctcgaagct
     4441 gcagcagtta cgaacttcaa cagcaaagca tgaaacgctc caacaggagg aagtatggcc
     4501 gctgccactt ctggttcaca accaagagct ggaaacccag ctccgagcag atggaccaag
     4561 accacagcag cagtgacagt tggaacaaca atgatgctgc tgcctccctg gagaactccg
     4621 cctcctccga cgaggaggac attggctccg agacgagagc catctactcc atcgtgctca
     4681 agcttccggg tcacagcacc atcctcaact ccaccaagtt accctcatcg gacaacctgc
     4741 aggtgcctga ggaggagctg gggatggtgg acttggagag gaaagccgac aagctgcagg
     4801 cccagaagag cgtggacgat ggaggcagtt ttccaaaaag cttctccaag cttcccatcc
     4861 agctagagtc agccgtggac acagctaaga cttctgacgt caactcctca gtgggtaaga
     4921 gcacggccac tctacctctg tccttcaagg aagccactct ggccaagagg tttgctctga
     4981 agaccagaag tcagatcact aagcggaaaa ggatgtccct ggtcaaggag aagaaagcgg
     5041 cccagaccct cagtgcgatc ttgcttgcct tcatcatcac ttggacccca tacaacatca
     5101 tggttctggt gaacaccttt tgtgacagct gcatacccaa aaccttttgg aatctgggct
     5161 actggctgtg ctacatcaac agcaccgtga accccgtgtg ctatgctctg tgcaacaaaa
     5221 cattcagaac cactttcaag atgctgctgc tgtgccagtg tgacaaaaaa aagaggcgca
     5281 agcagcagta ccagcagaga cagtcggtca tttttcacaa gcgcgcaccc gagcaggcct
     5341 tgaaggatcc cccggtcgcc accatggtga gcaagggcga ggaggataac atggccatca
     5401 tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg
     5461 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg
     5521 tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg
     5581 gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc
     5641 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga
     5701 cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca
     5761 acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg
     5821 agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga
     5881 aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc
     5941 agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact
     6001 acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg
     6061 agctgtacaa gtaatgaacg cgtgaattcg atatcaagct tatcgataat caacctctgg
     6121 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     6181 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     6241 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     6301 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     6361 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     6421 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     6481 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tatgttgcca
     6541 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     6601 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     6661 agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgagcg ctgctcgaga
     6721 gatctacggg tggcatccct gtgacccctc cccagtgcct ctcctggccc tggaagttgc
     6781 cactccagtg cccaccagcc ttgtcctaat aaaattaagt tgcatcattt tgtctgacta
     6841 ggtgtccttc tataatatta tggggtggag gggggtggta tggagcaagg ggcaagttgg
     6901 gaagacaacc tgtagggcct gcggggtcta ttgggaacca agctggagtg cagtggcaca
     6961 atcttggctc actgcaatct ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc
     7021 cgagttgttg ggattccagg catgcatgac caggctcagc taatttttgt ttttttggta
     7081 gagacggggt ttcaccatat tggccaggct ggtctccaac tcctaatctc aggtgatcta
     7141 cccaccttgg cctcccaaat tgctgggatt acaggcgtga accactgctc ccttccctgt
     7201 ccttctgatt ttgtaggtaa ccaggcgcgt gaattcactc ctcaggtgca ggctgcctat
     7261 cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca aataccactg agatcttttt
     7321 ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa
     7381 taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga
     7441 aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg
     7501 gcaacatatg cccatatgct ggctgccatg aacaaaggtt ggctataaag aggtcatcag
     7561 tatatgaaac agccccctgc tgtccattcc ttattccata gaaaagcctt gacttgaggt
     7621 tagatttttt ttatattttg ttttgtgtta tttttttctt taacatccct aaaattttcc
     7681 ttacatgttt tactagccag atttttcctc ctctcctgac tactcccagt catagctgtc
     7741 cctcttctct tatggagatc cctcgacctg cagcccaagc ttggcgtaat catggtcata
     7801 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     7861 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     7921 ctcactgccc gctttccagt cgggaaacct gtcgtgccag cggatcgaca gtactaagct
     7981 ttactaggga caggattggt gacagaaaag ccccatcctt aggcctcctc cttcctagtc
     8041 tcctgatatt gggtctaacc cccacctcct gttaggcaga ttccttatct ggtgacacac
     8101 ccccatttcc tggagccatc tctctccttg ccagaacctc taaggtttgc ttacgatgga
     8161 gccagagagg atcctgggag ggagagcttg gcagggggtg ggagggaagg gggggatgcg
     8221 tgacctgccc ggttctcagt ggccaccctg cgctaccctc tcccagaacc tgagctgctc
     8281 tgacgcggct gtctggtgcg tttcactgat cctggtgctg cagcttcctt acacttccca
     8341 agaggagaag cagtttggaa aaacaaaatc agaataagtt ggtcctgagt tctaactttg
     8401 gctcttcacc tttctagtcc ccaatttata ttgttcctcc gtgcgtcagt tttacctgtg
     8461 agataaggcc agtagccagc cccgtcctgg cagggctgtg gtgaggaggg gggtgtccgt
     8521 gtggaaaact ccctttgtga gaatggtgcg tcctaggtgt tcaccaggtc gtggccgcct
     8581 ctactccctt tctctttctc catccttctt tccttaaaga gtccccagtg ctatctggga
     8641 catattcctc cgcccagagc agggtcccgc ttccctaagg ccctgctctg ggcttctggg
     8701 tttgagtcct tggcaagccc aggagaggcg ctcaggcttc cctgtccccc ttcctcgtcc
     8761 accatctcat gcccctggct ctcctgcccc ttccctacag gggttcctgg ctctgctcta
     8821 agggcgaatt cgcggccgct aaattcaatt cgccctatag tgagtcgtat tacaattcac
     8881 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     8941 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     9001 cttcccaaca gttgcgcagc ctatacgtac ggcagtttaa ggtttacacc tataaaagag
     9061 agagccgtta tcgtctgttt gtggatgtac agagtgatat tattgacacg ccggggcgac
     9121 ggatggtgat ccccctggcc agtgcacgtc tgctgtcaga taaagtctcc cgtgaacttt
     9181 acccggtggt gcatatcggg gatgaaagct ggcgcatgat gaccaccgat atggccagtg
     9241 tgccggtctc cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca
     9301 aaaacgccat taacctgatg ttctggggaa tataaatgtc aggcatgaga ttatcaaaaa
     9361 ggatcttcac ctagatcctt ttcacgtaga aagccagtcc gcagaaacgg tgctgacccc
     9421 ggatgaatgt cagctactgg gctatctgga caagggaaaa cgcaagcgca aagagaaagc
     9481 aggtagcttg cagtgggctt acatggcgat agctagactg ggcggtttta tggacagcaa
     9541 gcgaaccgga attgccagct ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa
     9601 actggatggc tttcttgccg ccaaggatct gatggcgcag gggatcaagc tctgatcaag
     9661 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg
     9721 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
     9781 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
     9841 tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga
     9901 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
     9961 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
    10021 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
    10081 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
    10141 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
    10201 ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatgca tggcgatgcc
    10261 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
    10321 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
    10381 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
    10441 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gaattattaa cgcttacaat
    10501 ttcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catcaggtgg
    10561 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
    10621 tatgtatccg ctcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
    10681 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
    10741 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
    10801 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
    10861 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
    10921 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
    10981 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
    11041 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
    11101 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
    11161 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
    11221 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
    11281 tgaatactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
    11341 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    11401 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    11461 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    11521 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    11581 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    11641 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
    11701 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
    11761 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
    11821 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
    11881 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
    11941 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
    12001 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
    12061 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
    12121 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
    12181 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
    12241 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
    12301 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
    12361 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
    12421 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
    12481 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
    12541 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
    12601 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
    12661 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
    12721 gctcagaatt aaccctcact aaaggga
//
