LOCUS       sequence-212461-        9675 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To express human BLM protein in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9675)
  AUTHORS   Gaymes TJ, North PS, Brady N, Hickson ID, Mufti GJ, Rassool FV
  TITLE     Increased error-prone non homologous DNA end-joining--a proposed 
            mechanism of chromosomal instability in Bloom's syndrome.
  JOURNAL   Oncogene. 2002 Apr 11;21(16):2525-33. doi: 10.1038/sj.onc.1205331.
  PUBMED    11971187
REFERENCE   2  (bases 1 to 9675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/sj.onc"; 
            journalName: "Oncogene"; date: "2002-04-11- 11"; volume: "21"; 
            issue: "16"; pages: "2525-33"
COMMENT     Sequence Label: pcDNA3/BLM
FEATURES             Location/Qualifiers
     source          1..9675
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4940..4963
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(4994..5012)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4995..5012)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5032..5049)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5038..5262
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5308..5736
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5395..5414)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5605..5626
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5745..5765)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5750..6079
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5930..6065
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5992..6011
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6146..6940
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6200..6219)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6810..6829
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7114..7235
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7151..7170)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7205..7224
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7284..7300)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7284..7300)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7297..7319)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7308..7324
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7332..7338,7339..7356,7357..7362))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7377..7398
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7515..7532)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7686..8274)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7766..7785)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8445..9236,9237..9305))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9068..9087
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9306..9410)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9485..9504)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 ggtaccgagc tcggatccgc ccaggattat ggctgctgtt cctcaaaata atctacagga
      721 gcaactagaa cgtcactcag ccagaacact taataataaa ttaagtcttt caaaaccaaa
      781 attttcaggt ttcactttta aaaagaaaac atcttcagat aacaatgtat ctgtaactaa
      841 tgtgtcagta gcaaaaacac ctgtattaag aaataaagat gttaatgtta ccgaagactt
      901 ttccttcagt gaacctctac ccaacaccac aaatcagcaa agggtcaagg acttctttaa
      961 aaatgctcca gcaggacagg aaacacagag aggtggatca aaatcattat tgccagattt
     1021 cttgcagact ccgaaggaag ttgtatgcac tacccaaaac acaccaactg taaagaaatc
     1081 ccgggatact gctctcaaga aattagaatt tagttcttca ccagattctt taagtaccat
     1141 caatgattgg gatgatatgg atgactttga tacttctgag acttcaaaat catttgttac
     1201 accaccccaa agtcactttg taagagtaag cactgctcag aaatcaaaaa agggtaagag
     1261 aaactttttt aaagcacagc tttatacaac aaacacagta aagactgact tgcctccacc
     1321 ctcctctgaa agcgagcaaa tagatttgac tgaggaacag aaggatgact cagaatggtt
     1381 aagcagcgat gtgatttgca tcgatgatgg ccccattgct gaagtgcata taaatgaaga
     1441 tgctcaggaa agtgactctc tgaaaactca tttggaagat gaaagagata atagcgaaaa
     1501 gaagaagaat ttggaagaag ctgaattaca ttcaactgag aaagttccat gtattgaatt
     1561 tgatgatgat gattatgata cggattttgt tccaccttct ccagaagaaa ttatttctgc
     1621 ttcttcttcc tcttcaaaat gccttagtac gttaaaggac cttgacacct ctgacagaaa
     1681 agaggatgtt cttagcacat caaaagatct tttgtcaaaa cctgagaaaa tgagtatgca
     1741 ggagctgaat ccagaaacca gcacagactg tgacgctaga cagataagtt tacagcagca
     1801 gcttattcat gtgatggagc acatctgtaa attaattgat actattcctg atgataaact
     1861 gaaacttttg gattgtggga acgaactgct tcagcagcgg aacataagaa ggaaacttct
     1921 aacggaagta gattttaata aaagtgatgc cagtcttctt ggctcattgt ggagatacag
     1981 gcctgattca cttgatggcc ctatggaggg tgattcctgc cctacaggga attctatgaa
     2041 ggagttaaat ttttcacacc ttccctcaaa ttctgtttct cctggggact gtttactgac
     2101 taccacccta ggaaagacag gattctctgc caccaggaag aatctttttg aaaggccttt
     2161 attcaatacc catttacaga agtcctttgt aagtagcaac tgggctgaaa caccaagact
     2221 aggaaaaaaa aatgaaagct cttatttccc aggaaatgtt ctcacaagca ctgctgtgaa
     2281 agatcagaat aaacatactg cttcaataaa tgacttagaa agagaaaccc aaccttccta
     2341 tgatattgat aattttgaca tagatgactt tgatgatgat gatgactggg aagacataat
     2401 gcataattta gcagccagca aatcttccac agctgcctat caacccatca aggaaggtcg
     2461 gccaattaaa tcagtatcag aaagactttc ctcagccaag acagactgtc ttccagtgtc
     2521 atctactgct caaaatataa acttctcaga gtcaattcag aattatactg acaagtcagc
     2581 acaaaattta gcatccagaa atctgaaaca tgagcgtttc caaagtctta gttttcctca
     2641 tacaaaggaa atgatgaaga tttttcataa aaaatttggc ctgcataatt ttagaactaa
     2701 tcagctagag gcgatcaatg ctgcactgct tggtgaagac tgttttatcc tgatgccgac
     2761 tggaggtggt aagagtttgt gttaccagct ccctgcctgt gtttctcctg gggtcactgt
     2821 tgtcatttct cccttgagat cacttatcgt agatcaagtc caaaagctga cttccttgga
     2881 tattccagct acatatctga caggtgataa gactgactca gaagctacaa atatttacct
     2941 ccagttatca aaaaaagacc caatcataaa actcctatat gtcactccag aaaagatctg
     3001 tgcaagtaac agactcattt ctactctgga gaatctctat gagaggaagc tcttggcacg
     3061 ttttgttatt gatgaagcac attgtgtcag tcagtgggga catgattttc gtcaagatta
     3121 caaaagaatg aatatgcttc gccagaagtt tccttctgtt ccggtgatgg ctcttacggc
     3181 cacagctaat cccagggtac agaaggacat cctgactcag ctgaagattc tcagacctca
     3241 ggtgtttagc atgagcttta acagacataa tctgaaatac tatgtattac cgaaaaagcc
     3301 taaaaaggtg gcatttgatt gcctagaatg gatcagaaag caccacccat atgattcagg
     3361 gataatttac tgcctctcca ggcgagaatg tgacaccatg gctgacacgt tacagagaga
     3421 tgggctcgct gctcttgctt accatgctgg cctcagtgat tctgccagag atgaagtgca
     3481 gcagaagtgg attaatcagg atggctgtca ggttatctgt gctacaattg catttggaat
     3541 ggggattgac aaaccggacg tgcgatttgt gattcatgca tctctcccta aatctgtgga
     3601 gggttactac caagaatctg gcagagctgg aagagatggg gaaatatctc actgcctgct
     3661 tttctatacc tatcatgatg tgaccagact gaaaagactt ataatgatgg aaaaagatgg
     3721 aaaccatcat acaagagaaa ctcacttcaa taatttgtat agcatggtac attactgtga
     3781 aaatataaca gaatgcagga gaatacagct tttggcctac tttggtgaaa atggatttaa
     3841 tcctgatttt tgtaagaaac acccagatgt ttcttgtgat aattgctgta aaacaaagga
     3901 ttataaaaca agagatgtga ctgacgatgt gaaaagtatt gtaagatttg ttcaagaaca
     3961 tagttcatca caaggaatga gaaatataaa acatgtaggt ccttctggaa gatttactat
     4021 gaatatgctg gtcgacattt tcttggggag taagagtgca aaaatccagt caggtatatt
     4081 tggaaaagga tctgcttatt cacgacacaa tgccgaaaga ctttttaaaa agctgatact
     4141 tgacaagatt ttggatgaag acttatatat caatgccaat gaccaggcga tcgcttatgt
     4201 gatgctcgga aataaagcac aaactgtact aaatggcaat ttaaaggtag actttatgga
     4261 aacagaaaat tccagcagtg tgaaaaaaca aaaagcgtta gtagcaaaag tgtctcagag
     4321 ggaagagatg gttaaaaaat gtcttggaga acttacagaa gtctgcaaat ctctggggaa
     4381 agtttttggt gtccattact tcaatatttt taataccgtc actctcaaga agcttgcaga
     4441 atctttatct tctgatcctg aggttttgct tcaaattgat ggtgttactg aagacaaact
     4501 ggaaaaatat ggtgcggaag tgatttcagt attacagaaa tactctgaat ggacatcgcc
     4561 agctgaagac agttccccag ggataagcct gtccagcagc agaggccccg gaagaagtgc
     4621 cgctgaggag cttgacgagg aaatacccgt atcttcccac tactttgcaa gtaaaaccag
     4681 aaatgaaagg aagaggaaaa agatgccagc ctcccaaagg tctaagagga gaaaaactgc
     4741 ttccagtggt tccaaggcaa agggggggtc tgccacatgt agaaagatat cttccaaaac
     4801 gaaatcctcc agcatcattg gatccagttc agcctcacat acttctcaag cgacatcagg
     4861 agccaatagc aaattgggga ttatggctcc accgaagcct ataaatagac cgtttcttaa
     4921 gccttcatat gcattctcag actacaagga cgatgacgac aagtaactcg agcatgcatc
     4981 tagagggccc tattctatag tgtcacctaa atgctagagc tcgctgatca gcctcgactg
     5041 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     5101 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     5161 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     5221 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
     5281 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
     5341 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     5401 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     5461 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     5521 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     5581 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     5641 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     5701 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
     5761 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     5821 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     5881 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     5941 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     6001 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
     6061 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca
     6121 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
     6181 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     6241 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     6301 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
     6361 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     6421 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     6481 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     6541 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     6601 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     6661 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     6721 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     6781 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     6841 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     6901 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
     6961 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
     7021 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     7081 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
     7141 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     7201 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
     7261 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     7321 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     7381 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     7441 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     7501 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     7561 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     7621 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     7681 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     7741 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     7801 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     7861 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     7921 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     7981 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     8041 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     8101 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     8161 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     8221 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     8281 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     8341 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     8401 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     8461 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     8521 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     8581 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     8641 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     8701 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     8761 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     8821 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     8881 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     8941 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     9001 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     9061 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     9121 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     9181 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     9241 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     9301 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     9361 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     9421 cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtgcactc
     9481 tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt
     9541 tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg
     9601 acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg
     9661 ccagatatac gcgtt
//
