LOCUS       sequence-212465-        4048 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4048)
  AUTHORS   Bertrand E, Chartrand P, Schaefer M, Shenoy SM, Singer RH, Long RM
  TITLE     Localization of ASH1 mRNA particles in living yeast.
  JOURNAL   Mol Cell. 1998 Oct . 2(4):437-45.
  PUBMED    9809065
REFERENCE   2  (bases 1 to 4048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            1998 Oct . 2(4):437-45."
COMMENT     Sequence Label: pSL-MS2-12X
FEATURES             Location/Qualifiers
     source          1..4048
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_RNA        94..112
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        133..151
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        203..221
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        242..260
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        312..330
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        351..369
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        421..439
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        460..478
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        530..548
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        569..587
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        639..657
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        678..696
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     primer_bind     complement(954..971)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(954..970)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(963..985)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1183..1638
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1270..1289)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1480..1501
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1655..1674)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1774..1796
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1834..1852)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1920..2024
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2025..2093,2094..2885)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2243..2262)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3056..3644
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3545..3564
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3798..3815
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3932..3953
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3968..3973,3974..3991,3992..3998)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4006..4022
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4011..4033
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4030..4046
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4030..4046
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gattacgaat tcgaatggcc atgggacgtc gacctgaggt aattataacc cgggccctat
       61 atatggatcc taaggtacct aattgcctag aaaacatgag gatcacccat gtctgcaggt
      121 cgactctaga aaacatgagg atcacccatg tctgcagtat tcccgggttc attagatcct
      181 aaggtaccta attgcctaga aaacatgagg atcacccatg tctgcaggtc gactctagaa
      241 aacatgagga tcacccatgt ctgcagtatt cccgggttca ttagatccta aggtacctaa
      301 ttgcctagaa aacatgagga tcacccatgt ctgcaggtcg actccagaaa acatgaggat
      361 cacccatgtc tgcagtattc ccgggttcat tagatcctaa ggtacctaat tgcctagaaa
      421 acatgaggat cacccatgtc tgcaggtcga ctctagaaaa catgaggatc acccatgtct
      481 gcagtattcc cgggttcatt agatcctaag gtacctaatt gcctagaaaa catgaggatc
      541 acccatgtct gcaggtcgac tctagaaaac atgaggatca cccatgtctg cagtattccc
      601 gggttcatta gatcctaagg tacctaattg cctagaaaac atgaggatca cccatgtctg
      661 caggtcgact ccagaaaaca tgaggatcac ccatgtctgc agtattcccg ggttcattag
      721 atctgcgcgc gatcgatatc agcgctttaa atttgcgcat gctagctata gttctagagg
      781 taccggttgt taacgttagc cggctacgta tactccggaa tattaatagg cctaggatgc
      841 atatggcggc cgcctgcagc tggcgccatc gatacgcgta cgtcgcgacc gcggacatgt
      901 acagagctcg agaagtacta gtggccacgt gggccgtgca ccttaagctt ggcactggcc
      961 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     1021 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     1081 caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat
     1141 ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg
     1201 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     1261 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     1321 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     1381 accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     1441 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     1501 gaacaacact caaccctatc tcgggctatt cttttgattt ataagggatt ttgccgattt
     1561 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     1621 tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     1681 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     1741 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     1801 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
     1861 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     1921 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     1981 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     2041 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     2101 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     2161 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     2221 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     2281 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     2341 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     2401 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     2461 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     2521 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     2581 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     2641 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     2701 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     2761 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     2821 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     2881 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     2941 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     3001 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     3061 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     3121 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     3181 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     3241 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     3301 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     3361 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     3421 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     3481 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     3541 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     3601 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     3661 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     3721 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     3781 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     3841 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     3901 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     3961 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     4021 aatttcacac aggaaacagc tatgacat
//
