LOCUS       sequence-212467-        1682 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminally FLAG-tagged human RNMT in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Trotman JB, Giltmier AJ, Mukherjee C, Schoenberg DR
  TITLE     RNA guanine-7 methyltransferase catalyzes the methylation of 
            cytoplasmically recapped RNAs.
  JOURNAL   Nucleic Acids Res. 2017 Oct 13;45(18):10726-10739. doi: 
            10.1093/nar/gkx801.
  PUBMED    28981715
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2017-10-13- 13"; volume: "45"; issue: 
            "18"; pages: "10726-10739"
COMMENT     Sequence Label: pcDNA3-FLAG-RNMT
FEATURES             Location/Qualifiers
     source          1..1682
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     76..95
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        76..94
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             115..138
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             151..162
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac ccactgctta
       61 ctggcttatc gaaattaata cgactcacta tagggagacc caagcttgat gggcgactac
      121 aaagacgatg acgacaagag cagcggccat atcgaaggtc gtcatatggc aaattctgca
      181 aaagcagaag aatatgaaaa gatgtctctt gaacaggcaa aagcgtcagt gaattctgaa
      241 acagagtctt cattcaatat taatgaaaac acaacagctt ctgggactgg gctttctgaa
      301 aagacttctg tctgtaggca agtagacata gcaagaaaga gaaaagagtt tgaagatgat
      361 cttgtaaagg aaagttctag ttgtgggaaa gacactccat ccaagaagag aaaacttgat
      421 cctgaaattg tcccagagga aaaagattgt ggtgatgctg aaggcaattc aaagaaaaga
      481 aaaagagaaa ctgaggatgt tccaaaagat aaatcttcta ctggagatgg cactcaaaat
      541 aagagaaaaa tagcacttga ggatgttcct gaaaagcaga aaaatctgga agaaggacac
      601 agctcaacag tggctgccca ttacaatgaa cttcaggaag ttggtttgga gaagcgtagt
      661 caaagtcgta ttttttacct aagaaacttt aataattgga tgaaaagtgt tctcattgga
      721 gaatttttgg aaaaggtacg acagaagaaa aaacgtgata tcactgtttt ggacctggga
      781 tgtggtaaag gtggagattt gctgaaatgg aaaaaaggaa gaattaacaa gctagtttgt
      841 actgatattg ccgatgtttc tgtcaaacag tgtcagcagc ggtatgagga catgaaaaat
      901 cgtcgtgata gtgaatatat tttcagtgca gaatttataa ctgctgacag ctcaaaggaa
      961 cttctgattg acaaatttcg tgacccacaa atgtgttttg acatctgcag ttgtcagttt
     1021 gtctgtcatt actcatttga gtcttatgag caggctgaca tgatgctgag aaatgcgtgt
     1081 gagagactta gccctggggg ctattttatt ggtactactc ccaatagctt tgaattgata
     1141 agacgccttg aagcttcaga aacagaatca tttggaaatg aaatatatac tgtgaaattt
     1201 cagaagaaag gagattatcc tttatttggc tgcaaatatg acttcaactt ggaaggtgtt
     1261 gtggatgttc ctgaattctt ggtctatttt ccattgctaa atgaaatggc aaagaagtac
     1321 aatatgaaac tagtctacaa aaaaacattt ctggaattct acgaagaaaa gattaagaac
     1381 aatgaaaata aaatgctctt aaaacgaatg caggccttgg agccatatcc tgcaaatgag
     1441 agttctaaac ttgtctctga gaaggtggat gactatgaac atgcagcaaa gtacatgaag
     1501 aacagtcaag taaggttacc tttgggaacc ttaagtaaat cagaatggga agctacaagt
     1561 atttacttgg tgtttgcctt tgagaaacag cagtgatgac gagctcggat ccactagtaa
     1621 cggccgccag tgtgctggaa ttctgcagat atccatcaca ctggcggccg ctcgagcatg
     1681 ca
//
