LOCUS       sequence-212473-        5504 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ATF1 from Saccharomyces cerevisiae under osmY stationary phase 
            promoter; kanamycin resistant..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5504)
  AUTHORS   Siddall A, Williams AA, Sanders J, Denton JA, Madden D, Schollar J, 
            Bryk J
  TITLE     UNIGEMS: plasmids and parts to facilitate teaching on assembly, gene
            expression control and logic in E. coli
  JOURNAL   bioRxiv 2021.06.20.449138
REFERENCE   2  (bases 1 to 5504)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.06.20.449138"
COMMENT     Sequence Label: p006-Banana-Late
FEATURES             Location/Qualifiers
     source          1..5504
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      476..1064
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     965..984
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1218..1235
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1333..1350
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1334..1350
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      3523..3594
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3610..3637
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(3742..3761)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     promoter        3828..3919
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3920..4729
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             join(4812..5504,1..390)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(4831..4850)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg
       61 ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg
      121 ggatacgacg ataccgaaga tagctcatgt tatatcccgc cgttaaccac catcaaacag
      181 gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag
      241 gcggtgaagg gcaatcagct gttgccagtc tcactggtga aaagaaaaac caccctggcg
      301 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
      361 caggtttccc gactggaaag cgggcagtga ctcatgacca aaatccctta acgtgagtta
      421 cgcgcgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
      481 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
      541 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
      601 gagcgcagat accaaatact gttcttctag tgtagccgta gttagcccac cacttcaaga
      661 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
      721 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
      781 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
      841 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
      901 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
      961 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     1021 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     1081 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     1141 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     1201 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaaggcgag agtagggaac
     1261 tgccaggcat caaactaagc agaaggcccc tgacggatgg cctttttgcg tttctacaaa
     1321 ctctttctgt gttgtaaaac gacggccagt cttaagctcg ggccccctgg gcggttctga
     1381 taacgagtaa tcgttaatcc gcaaataacg taaaaacccg cttcggcggg tttttttatg
     1441 gggggagttt agggaaagag catttgtcag aatatttaag ggcgcctgtc actttgcttg
     1501 atatatgaga attatttaac cttataaatg agaaaaaagc aacgcacttt aaataagata
     1561 cgttgctttt tcgattgatg aacacctata attaaactat tcatctatta tttatgattt
     1621 tttgtatata caatatttct agtttgttaa agagaattaa gaaaataaat ctcgaaaata
     1681 ataaagggaa aatcagtttt ctggcacagg aacgttatcc ggacgttcag ttccaccaga
     1741 cccgcgagca ttaattcttg cctccagggc gcggtagtgg cgccctgtca atttcccttc
     1801 cttattagcc gcttacggaa tgttcttaaa acattcactt ttgcttatgt tttcgctgat
     1861 atcccgagcg gtttcaaaat tgtgatctat atttaacaat actagagaaa gaggggaaat
     1921 actagatgaa tgaaatcgat gagaaaaatc aggcccccgt gcaacaagaa tgcctgaaag
     1981 agatgattca gaatgggcat gctcggcgta tgggatctgt tgaagatctg tatgttgctc
     2041 tcaacagaca aaacttatat cgaaacttct gcacatatgg agaattgagt gattactgta
     2101 ctagggatca gctcacatta gctttgaggg aaatctgcct gaaaaatcca actcttttac
     2161 atattgttct accaacaaga tggccaaatc atgaaaatta ttatcgcagt tccgaatact
     2221 attcacggcc acatccagtg catgattata tttcagtatt acaagaattg aaactgagtg
     2281 gtgtggttct caatgaacaa cctgagtaca gtgcagtaat gaagcaaata ttagaagagt
     2341 tcaaaaatag taagggttcc tatactgcaa aaatttttaa acttactacc actttgacta
     2401 ttccttactt tggaccaaca ggaccgagtt ggcggctaat ttgtcttcca gaagagcaca
     2461 cagaaaagtg gaaaaaattt atctttgtat ctaatcattg catgtctgat ggtcggtctt
     2521 cgatccactt ttttcatgat ttaagagacg aattaaataa tattaaaact ccaccaaaaa
     2581 aattagatta cattttcaag tacgaggagg attaccaatt attgaggaaa cttccagaac
     2641 cgatcgaaaa ggtgatagac tttagaccac cgtacttgtt tattccgaag tcacttcttt
     2701 cgggtttcat ctacaatcat ttgagatttt cttcaaaagg tgtctgtatg agaatggatg
     2761 atgtggaaaa aaccgatgat gttgtcaccg agatcatcaa tatttcacca acagaatttc
     2821 aagcgattaa agcaaatatt aaatcaaata tccaaggtaa gtgtactatc actccgtttt
     2881 tacatgtttg ttggtttgta tctcttcata aatggggtaa atttttcaaa ccattgaact
     2941 tcgaatggct tacggatatt tttatccccg cagattgccg ctcacaacta ccagatgatg
     3001 atgaaatgag acagatgtac agatatggcg ctaacgttgg atttattgac ttcaccccct
     3061 ggataagcga atctgacatg aatgataaca aagaaaattt ttggccactt attgagcact
     3121 accatgaagt aatttcggaa gctttaagaa ataaaaagca tctccatggc ttagggttca
     3181 atatacaagg cttcgttcaa aaatatgtga acattgacaa ggtaatgtgc gatcgtgcca
     3241 tcgggaaaag acgcggaggt acattgttaa gcaatgtagg tctgtttaat cagttagagg
     3301 agcccgatgc caaatattct atatgcgatt tggcatttgg ccaatttcaa ggatcctggc
     3361 accaagcatt ttccttgggt gtttgttcga ctaatgtaaa ggggatgaat attgttgttg
     3421 cttcaacaaa gaatgttgtt ggtagtcaag aatctctcga agagctttgc tccatttaca
     3481 aagctctcct tttaggccct taataatact agagccaggc atcaaataaa acgaaaggct
     3541 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
     3601 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atacatgggg agtccccaca
     3661 ctaccatcgg cgctacggcg tttcacttct gagttcggca tggggtcagg tgggaccacc
     3721 gcgctactgc cgccaggcaa acaaggggtg ttatgagcca tattcaggta taaatgggct
     3781 cgcgataatg ttcagaattg gttaattggt tgtaacactg acccctattt gtttattttt
     3841 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     3901 atattgaaaa aggaagaata tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa
     3961 ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc
     4021 aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca
     4081 tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac
     4141 ggaatttatg ccacttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt
     4201 actcaccact gcgatccccg gaaaaacagc gttccaggta ttagaagaat atcctgattc
     4261 aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcact cgattcctgt
     4321 ttgtaattgt ccttttaaca gcgatcgcgt atttcgcctc gctcaggcgc aatcacgaat
     4381 gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga
     4441 acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca
     4501 tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga
     4561 tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct
     4621 cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc
     4681 tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaag cggcgcgcca
     4741 tcgaatggcg caaaaccttt cgcggtatgg catgatagcg cccggaagag agtcaattca
     4801 gggtggtgaa tatgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt
     4861 atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa
     4921 aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca caacaactgg
     4981 cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt
     5041 cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt
     5101 cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc
     5161 aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg
     5221 aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccatca
     5281 acagtattat tttctcccat gaggacggta cgcgactggg cgtggagcat ctggtcgcat
     5341 tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc
     5401 gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg
     5461 aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaa
//
