LOCUS       sequence-212475-       10557 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains mouse kappa CL domain, for cloning of antibody VL domains 
            using SapI restriction enzyme..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10557)
  AUTHORS   Vazquez-Lombardi R, Nevoltris D, Rouet R, Christ D
  TITLE     Expression of IgG Monoclonals with Engineered Immune Effector 
            Functions.
  JOURNAL   Methods Mol Biol. 2018;1827:313-334. doi: 
            10.1007/978-1-4939-8648-4_16.
  PUBMED    30196504
REFERENCE   2  (bases 1 to 10557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2018"; volume: "1827:313-334. doi"; pages: " 
            10.1007/978-1-4939-8648-4_16"
COMMENT     Sequence Label: pRVL-1
FEATURES             Location/Qualifiers
     source          1..10557
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        384..587
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     538..558
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     584..608
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      629..638
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    1041..1175
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1065..1084)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1119..1138
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1318..1340)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      1900..3689
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(3991..5916)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGGGAGGGAGAGGGAGGAGAGGGX
                     GGAGGAGAGGGAGAGGGAGGXGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(6351..6369)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6437..6541
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6542..6610,6611..7402)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6760..6779)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7573..8161
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8062..8081
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        8592..8737
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             8780..9817
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(9815..9834)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9859..9907
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat
      421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc
      541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
      601 gtcagatctc tagaagctgg gtaccgccgc caccatgggc tggagctgca tcatcctgtt
      661 cctggtggcc accgccaccg gcgtgcacag cgaagagcta cagagtgagc tcttcgccga
      721 cgccgccccc accgtgagca tcttcccccc cagcagcgag cagctgacca gcggcggcgc
      781 cagcgtggtg tgcttcctga acaacttcta ccccaaggac atcaacgtga agtggaagat
      841 cgacggcagc gagaggcaga acggcgtgct gaacagctgg accgaccagg acagcaagga
      901 cagcacctac agcatgagca gcaccctgac cctgaccaag gacgagtacg agaggcacaa
      961 cagctacacc tgcgaggcca cccacaagac cagcaccagc cccatcgtga aaagcttcaa
     1021 caggaacgag tgctgataag gatccagaca tgataagata cattgatgag tttggacaaa
     1081 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
     1141 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
     1201 tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat
     1261 gtggtatggc tgattatgat ccggctgcct cgcgcgtttc ggtgatgacg gtgaaaacct
     1321 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     1381 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaggtc
     1441 gactctagag gatcgatccc cgccccggac gaactaaacc tgactacgac atctctgccc
     1501 cttcttcgcg gggcagtgca tgtaatccct tcagttggtt ggtacaactt gccaactggg
     1561 ccctgttcca catgtgacac ggggggggac caaacacaaa ggggttctct gactgtagtt
     1621 gacatcctta taaatggatg tgcacatttg ccaacactga gtggctttca tcctggagca
     1681 gactttgcag tctgtggact gcaacacaac attgccttta tgtgtaactc ttggctgaag
     1741 ctcttacacc aatgctgggg gacatgtacc tcccaggggc ccaggaagac tacgggaggc
     1801 tacaccaacg tcaatcagag gggcctgtgt agctaccgat aagcggaccc tcaagagggc
     1861 attagcaata gtgtttataa ggcccccttg ttaaccctaa acgggtagca tatgcttccc
     1921 gggtagtagt atatactatc cagactaacc ctaattcaat agcatatgtt acccaacggg
     1981 aagcatatgc tatcgaatta gggttagtaa aagggtccta aggaacagcg atatctccca
     2041 ccccatgagc tgtcacggtt ttatttacat ggggtcagga ttccacgagg gtagtgaacc
     2101 attttagtca caagggcagt ggctgaagat caaggagcgg gcagtgaact ctcctgaatc
     2161 ttcgcctgct tcttcattct ccttcgttta gctaatagaa taactgctga gttgtgaaca
     2221 gtaaggtgta tgtgaggtgc tcgaaaacaa ggtttcaggt gacgccccca gaataaaatt
     2281 tggacggggg gttcagtggt ggcattgtgc tatgacacca atataaccct cacaaacccc
     2341 ttgggcaata aatactagtg taggaatgaa acattctgaa tatctttaac aatagaaatc
     2401 catggggtgg ggacaagccg taaagactgg atgtccatct cacacgaatt tatggctatg
     2461 ggcaacacat aatcctagtg caatatgata ctggggttat taagatgtgt cccaggcagg
     2521 gaccaagaca ggtgaaccat gttgttacac tctatttgta acaaggggaa agagagtgga
     2581 cgccgacagc agcggactcc actggttgtc tctaacaccc ccgaaaatta aacggggctc
     2641 cacgccaatg gggcccataa acaaagacaa gtggccactc ttttttttga aattgtggag
     2701 tgggggcacg cgtcagcccc cacacgccgc cctgcggttt tggactgtaa aataagggtg
     2761 taataacttg gctgattgta accccgctaa ccactgcggt caaaccactt gcccacaaaa
     2821 ccactaatgg caccccgggg aatacctgca taagtaggtg ggcgggccaa gataggggcg
     2881 cgattgctgc gatctggagg acaaattaca cacacttgcg cctgagcgcc aagcacaggg
     2941 ttgttggtcc tcatattcac gaggtcgctg agagcacggt gggctaatgt tgccatgggt
     3001 agcatatact acccaaatat ctggatagca tatgctatcc taatctatat ctgggtagca
     3061 taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta
     3121 tatgctatcc taatttatat ctgggtagca taggctatcc taatctatat ctgggtagca
     3181 tatgctatcc taatctatat ctgggtagta tatgctatcc taatctgtat ccgggtagca
     3241 tatgctatcc taatagagat tagggtagta tatgctatcc taatttatat ctgggtagca
     3301 tatactaccc aaatatctgg atagcatatg ctatcctaat ctatatctgg gtagcatatg
     3361 ctatcctaat ctatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg
     3421 ctatcctaat ctatatctgg gtagtatatg ctatcctaat ttatatctgg gtagcatagg
     3481 ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg
     3541 ctatcctaat ctgtatccgg gtagcatatg ctatcctcat gcatatacag tcagcatatg
     3601 atacccagta gtagagtggg agtgctatcc tttgcatatg ccgccacctc ccaagggggc
     3661 gtgaattttc gctgcttgtc cttttcctgc tggttgctcc cattcttagg tgaatttaag
     3721 gaggccaggc taaagccgtc gcatgtctga ttgctcacca ggtaaatgtc gctaatgttt
     3781 tccaacgcga gaaggtgttg agcgcggagc tgagtgacgt gacaacatgg gtatgcccaa
     3841 ttgccccatg ttgggaggac gaaaatggtg acaagacaga tggccagaaa tacaccaaca
     3901 gcacgcatga tgtctactgg ggatttattc tttagtgcgg gggaatacac ggcttttaat
     3961 acgattgagg gcgtctccta acaagttaca tcactcctgc ccttcctcac cctcatctcc
     4021 atcacctcct tcatctccgt catctccgtc atcaccctcc gcggcagccc cttccaccat
     4081 aggtggaaac cagggaggca aatctactcc atcgtcaaag ctgcacacag tcaccctgat
     4141 attgcaggta ggagcgggct ttgtcataac aaggtcctta atcgcatcct tcaaaacctc
     4201 agcaaatata tgagtttgta aaaagaccat gaaataacag acaatggact cccttagcgg
     4261 gccaggttgt gggccgggtc caggggccat tccaaagggg agacgactca atggtgtaag
     4321 acgacattgt ggaatagcaa gggcagttcc tcgccttagg ttgtaaaggg aggtcttact
     4381 acctccatat acgaacacac cggcgaccca agttccttcg tcggtagtcc tttctacgtg
     4441 actcctagcc aggagagctc ttaaaccttc tgcaatgttc tcaaatttcg ggttggaacc
     4501 tccttgacca cgatgctttc caaaccaccc tccttttttg cgcctgcctc catcaccctg
     4561 accccggggt ccagtgcttg ggccttctcc tgggtcatct gcggggccct gctctatcgc
     4621 tcccgggggc acgtcaggct caccatctgg gccaccttct tggtggtatt caaaataatc
     4681 ggcttcccct acagggtgga aaaatggcct tctacctgga gggggcctgc gcggtggaga
     4741 cccggatgat gatgactgac tactgggact cctgggcctc ttttctccac gtccacgacc
     4801 tctccccctg gctctttcac gacttccccc cctggctctt tcacgtcctc taccccggcg
     4861 gcctccacta cctcctcgac cccggcctcc actacctcct cgaccccggc ctccactgcc
     4921 tcctcgaccc cggcctccac ctcctgctcc tgcccctcct gctcctgccc ctcctcctgc
     4981 tcctgcccct cctgcccctc ctgctcctgc ccctcctgcc cctcctgctc ctgcccctcc
     5041 tgcccctcct gctcctgccc ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc
     5101 tcctgctcct gcccctcctg cccctcctgc tcctgcccct cctgcccctc ctgctcctgc
     5161 ccctcctgcc cctcctgctc ctgcccctcc tgctcctgcc cctcctgctc ctgcccctcc
     5221 tgctcctgcc cctcctgccc ctcctgcccc tcctcctgct cctgcccctc ctgctcctgc
     5281 ccctcctgcc cctcctgccc ctcctgctcc tgcccctcct cctgctcctg cccctcctgc
     5341 ccctcctgcc cctcctcctg ctcctscccc tcctgcccct cctcctgctc ctgcccctcc
     5401 tcctgctcct gcccctcctg cccctcctsc ccctcctcct gctcctgccc ctcctgcccc
     5461 tcctcctgct cctgcccctc ctcctgctcc tccccctcct gcccctcctg cccctcctcc
     5521 tgctcctgcc cctcctcctg ctcctgcccc tcctgcccct cctgcccctc ctgcccctcc
     5581 tcctgctcct gcccctcctc ctgctcctgc ccctcctgct cctgcccctc ccgctcctgc
     5641 tcctgctcct gttccaccgt gggtcccttt gcagccaatg caacttggac gtttttgggg
     5701 tctccggaca ccatctctat gtcttggccc tgatcctgag ccgcccgggg ctcctggtct
     5761 tccgcctcct cgtcctcgtc ctcttccccg tcctcgtcca tggttatcac cccctcttct
     5821 ttgaggtcca ctgccgccgg agccttctgg tccagatgtg tctcccttct ctcctaggcc
     5881 atttccaggt cctgtacctg gcccctcgtc agacatgatt cacactaaaa gagatcaata
     5941 gacatcttta ttagacgacg ctcagtgaat acagggagtg cagactcctg ccccctccaa
     6001 cagccccccc accctcatcc ccttcatggt cgctgtcaga cagatccagg tctgaaaatt
     6061 ccccatcctc cgaaccatcc tcgtcctcat caccaattac tcgcagcccg gaaaactccc
     6121 gctgaacatc ctcaagattt gcgtcctgag cctcaagcca ggcctcaaat tcctcgtccc
     6181 cctttttgct ggacggtagg gatggggatt ctcgggaccc ctcctcttcc tcttcaaggt
     6241 caccagacag agatgctact ggggcaacgg aagaaaagct gggtgcggcc tgtgaggatc
     6301 agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat
     6361 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     6421 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     6481 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     6541 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     6601 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     6661 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     6721 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     6781 ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     6841 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     6901 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     6961 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     7021 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     7081 gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     7141 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     7201 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     7261 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     7321 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     7381 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     7441 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     7501 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     7561 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     7621 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     7681 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     7741 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     7801 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     7861 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     7921 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     7981 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     8041 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     8101 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     8161 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt gaagctgtcc
     8221 ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc
     8281 gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag
     8341 caagacgtag cccagcgcgt cggccccgag atgcgccgcg tgcggctgct ggagatggcg
     8401 gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat
     8461 tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccc tgcttcatcc
     8521 ccgtggcccg ttgctcgcgt ttgctggcgg tgtccccgga agaaatatat ttgcatgtct
     8581 ttagttctat gatgacacaa accccgccca gcgtcttgtc attggcgaat tcgaacacgc
     8641 agatgcagtc ggggcggcgc ggtccgaggt ccacttcgca tattaaggtg acgcgtgtgg
     8701 cctcgaacac cgagcgaccc tgcagcgacc cgcttaacag cgtcaacagc gtgccgcaga
     8761 tcccgggggg caatgagata tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt
     8821 tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc
     8881 tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc
     8941 cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat
     9001 tccggaagtg cttgacattg gggaattcag cgagagcctg acctattgca tctcccgccg
     9061 tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc
     9121 ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg
     9181 cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat
     9241 tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt
     9301 cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct
     9361 cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt
     9421 cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt
     9481 ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc
     9541 ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc ttgaccaact
     9601 ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga
     9661 cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc
     9721 ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag
     9781 cactcgtccg gatcgggaga tgggggaggc taactgaaac acggaaggag acaataccgg
     9841 aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggg tgttgggtcg
     9901 tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga
     9961 ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg
    10021 ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat agccactggc
    10081 cccgtgggtt agggacgggg tcccccatgg ggaatggttt atggttcgtg ggggttatta
    10141 ttttgggcgt tgcgtggggt caggtccacg actggactga gcagacagac ccatggtttt
    10201 tggatggcct gggcatggac cgcatgtact ggcgcgacac gaacaccggg cgtctgtggc
    10261 tgccaaacac ccccgacccc caaaaaccac cgcgcggatt tctggcgtgc caagctagtc
    10321 gaccaattct catgtttgac agcttatcat cgcagatccg ggcaacgttg ttgccattgc
    10381 tgcaggcgca gaactggtag gtatggaaga tctatacatt gaatcaatat tggcaattag
    10441 ccatattagt cattggttat atagcataaa tcaatattgg ctattggcca ttgcatacgt
    10501 tgtatctata tcataatatg tacatttata ttggctcatg tccaatatga ccgccat
//
