LOCUS       sequence-212481-        8688 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the C. elegans unc-31 cDNA pan-neuronally.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8688)
  AUTHORS   Charlie NK, Schade MA, Thomure AM, Miller KG
  TITLE     Presynaptic UNC-31 (CAPS) is required to activate the G alpha(s) 
            pathway of the Caenorhabditis elegans synaptic signaling network.
  JOURNAL   Genetics. 2006 Feb;172(2):943-61. doi: 10.1534/genetics.105.049577. 
            Epub 2005 Nov 4.
  PUBMED    16272411
REFERENCE   2  (bases 1 to 8688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.105.049577"; journalName: "Genetics"; date: 
            "2006-02"; volume: "172"; issue: "2"; pages: "943-61"
COMMENT     Sequence Label: KG#121
FEATURES             Location/Qualifiers
     source          1..8688
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(6111..6133)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6233..6252
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6330..6348)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6416..6520
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6521..6589,6590..7381)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6739..6758)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7552..8140
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8041..8060
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8294..8311
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8428..8449
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8464..8469,8470..8487,8488..8494)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8502..8518
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8507..8529
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8526..8542
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8526..8542
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tcttcagatg ggagcagtgg actgtctatt tgatattctt aaaatgtact ctataaaaga
       61 tgaaactgta atgaccaatt ccctacataa atatttttaa ctaattggtc ggatatttgg
      121 ggatcagaga agtacttgtt tttgcgatat taataatgat atatttttct agtaaaaaaa
      181 tgttaagcga tttgtgaata attaagttgg cttgaaaaag attttttgat agctatgtat
      241 tttgttaatt taaatcagat tttaaaactt tattattgac tatttatata tattatatat
      301 tatatatttt catggtttaa tcaaaatcta ttaaattatt tctaaaatct ctaaatatcg
      361 aattctctaa aaagcttcat caacaaaagc atttcaatta tctaacacaa tgagaataat
      421 tcaagaactg aaatagaaag aaatctagaa aaaaaagaga gtgaggtgcc ctattaaatc
      481 ggtgctctcc tctctaacag aagcgcctac acgcacccaa gagagctctt tttctttctt
      541 ttttcggagc acacatacac gcatacactc ctcagaagtg tatagagaaa atgggagaag
      601 aagcgaaaga agacgaagaa gcggttgtga tgtatgcatc gggcgtttct atctctctcc
      661 gtgagcaacg agctagtcaa cccatcgatc catttttgtg agcacacaca gagagagact
      721 caaacacact cacagcttca cactgagctc caagagctct gtagagagaa gccagagtat
      781 gctttcctcg attttcctaa cgtcttcttc ttctgctttt ttctttttcc caacgagttg
      841 tagtccacta acttcaattt ttcctccaga gaaagagttt tagagagaca gctgataaat
      901 acactagaga aataaaaaga atgataaaaa tttgctattt taatcagttc cctctcgttt
      961 ctctaagttt tgttgttggg ttgggaaatt aaaattggaa aatggaaata acgttttttt
     1021 ctgtctaatc ttgaattctc cagacatttc ttatttcctt ttttgttgca agtttttctt
     1081 ccttctctaa tttttaatca aagatatctc gctatgaact ttgaattttt gatattttga
     1141 ttctataaat acataaagta atctaaatgc tcttttaaaa taaatctaca gtagccctat
     1201 tttcagggat ccccgggatt ggccaaagga cccaaaggta tgtttcgaat gatactaaca
     1261 taacatagaa cattttcagg aggacccttg gctagcaaaa atgtcgaatg tttcaaagcc
     1321 gataatgcaa aattcacaag aattcgatgg agatgaggaa gaaggggaag aaggttcaac
     1381 tacagtagtc ggtagtggag atgaatgtgg tggtcctgct ttgagcaaag aagagcagga
     1441 aagaatgaaa gccgaacgag aggaagaaga tcataagaag aatcttcaaa tgtacatgtt
     1501 tctagccaga tgcatagcat atccattcaa tggacagcag actggtgaca tggctcgtcg
     1561 acaaatgaaa gtgaataaac aggaattggc tagaattcga gaacgattca ctctttttct
     1621 taaaggagaa acaaatattg ctgctgatga agcattcact aaagcgattc aaagttattt
     1681 cgaagtattt ctgaaatctg aaagagttca gaaagttgtt cacgccggcg gattttcaca
     1741 acatgatttt cgagaagttt ttcgattaaa tattgagaaa agggttcgaa gtcttcctga
     1801 tattgaagga ttatcaaagg atactgtatt gaattcttgg ttagcaaaat tcgatgcaat
     1861 tattaaagga gatgagactg atcagaatag gaatgctcga ggccgctctc gaaatccgca
     1921 gaatgcgatg agcgctgacg cagtactagg caaagagcaa ctctacgacg tttttcaaca
     1981 aattcttggt gtgaagaaat tcgagcatca aatcatattc aacgcacttc agttggataa
     2041 tccagatgag caggcggcag caatccggag agagtttgcg acaagagaag aagctttgaa
     2101 ggatcctatc aagatgaagc gtcttactcc aaaatttgtg gtaaaagaca tggaaaccct
     2161 gtacatggac gaagttcgaa tgtcgataaa cactttaata ggaaatttgg aaacagttcc
     2221 agtgacaaca cgaggggcaa ctgtcggaaa acgaaaagac aagagtcgat cccgcagtat
     2281 agaggatttg tcgctcttca acagccttaa acgaaggaca agctccggga gtctcaacaa
     2341 aggagatagt gaagatggtg acgtcacact gacaaagtct gatgtctcat tggctttgag
     2401 tatggaggtt gtcgtgatgg aagtgcaggg tctaaaatcc gtgcaaccca gtaaaatagt
     2461 gtattgcacg atggaagtag acggtcacaa gctgcagaca gatcatgcgg aggcttccaa
     2521 accaaaatgg gatacacaag gagacttttc gacgaaaaat ccattgcccg tggtgaaagt
     2581 gaaattgtat acagaagtga agagtatgat agcatttgag gataaagaat taggaaaagt
     2641 aataatccag ccgacgccaa actgtgcgcg aagtccagaa tggtacacca tgacattacc
     2701 aaaaagtagt caggatcaga atttaaagat taggatagca attcgagtag aaaaaccgcc
     2761 gaatttaaag tattgcggtt attgttattg tatcgggcga aacgcgtgga agaagtggaa
     2821 gaagcgattc ttttgtcttg ttcaagtatc tcagtacgca tttgctgtat gtagttttcg
     2881 acaaaagaag gccgatccca cggaattcat tcaattagac gggtttacga tcgattatat
     2941 gccggagtcg gatccagaac tctctgcgca aggaggaaaa cacttcttca ctgccatcaa
     3001 agaaggagac gagctgaaat ttgcgactga tgacgaaaat gagcgacatc tatgggtcca
     3061 ggctctgtac cgagccacag gtcaagccta taaacctgtg cctccaaagc agtcaacaat
     3121 tgctccaaag gctcaaggtt tccaggataa agccagcaaa catggaatgg atgcaatgat
     3181 tcaggctgat tcgattaatt tcgatcatga ccatttttat tccgatgttc aacgattaac
     3241 tctggatttt cggatcaacg agccgatttg ttcattgggt tggttctcac ctggtcaagc
     3301 atttgttctg gaagaatact ctgctcgata tatggttcgt ggatgcttcc gccacgtaac
     3361 cctcttatca aatcttttgg ataaagctga cgatggattg ctcattgatc cagctctaat
     3421 tcattactct ttcgcttttt gtgcatctca tgtccatgga aataggtgta tgcctgatcg
     3481 acaaggacct gaaggagttg gtacagtaac tctggaggag aaggaaaagt tccaggaaat
     3541 caaggaacga ttgagagtac ttctagagaa gcaaatcacg aatttccgtt attgtttccc
     3601 atttggaaga cctgaaggcg cacttaaagg gacattaggt ctattggaaa gagttttgat
     3661 gaaggatgtt gtgtcgccag ttccaccgga agaagttcga gcagttatta gaaaatgttt
     3721 ggaggatgct gcattggtta attacacaag gatttgcaat gaagcaaaga ttgaacaacg
     3781 aatgggtatt gatgttagtc cggctcaacg aatcgaggac atgatccgcg tgacggaatt
     3841 ctgcattgat ctgctcaagg agaacgagga gcatcatgga gaggctttcg cgtggttttc
     3901 cgaccttctt tccgaccatt ccgagatctt ttggtcattg tattcagtcg acttggactc
     3961 ggcactggaa gttcaaccac acgatagttg ggattctttc cctcttttcc agatgctcaa
     4021 cgactttttg ctgtctgaat caagcctgaa aggaggtata ttccacaaca aaatcgttca
     4081 acaattccaa cctctcgttg tacgatacat tgacctcatg gagcactcga ttgctcaggc
     4141 gattgacaaa ggattttcca aggaaaaatg ggaatcaaga aaagaaggat gtgcaacttc
     4201 ggaagatatt tactggaagc tcgacgctct tcacacattt gtcatcgatc tgaactggcc
     4261 tgaagaggat ttcagaaaat atttgcagac gaaaatgaaa agtctaactt ctgatatgat
     4321 atccaaggtt tccgattgca ccttcaccgc attcgacagt tggatgcaac gagccaaaaa
     4381 gtccaccgat tacatgcttc catcagaagt ttgcgtgcaa atcaacgtaa tgttcagctc
     4441 aaagagccgt gctgttcgag tcactgttga ttctggagaa tacaaatatc agtcaaaact
     4501 cgacgaaact ctggagacaa tgctcaaaac tatggaatca tgtattcaag agaaacttca
     4561 cggagttctt gaatcagtgc tctcacgtct tgctcgatac gacgagggaa atccgattgg
     4621 agcaattttg aatattgccc caaagcctgc atcgattttc aacaaattga agacaatggc
     4681 cggagacacc tcggctcagg caacgacgac agcgcgtcaa cctctgactg ctcaacaatc
     4741 atcagggcaa atcggaaatt catatgttac atttttccac ggatgcactg aacttcttcg
     4801 acaagttatt atcgatgaaa tttgggttaa tgggctcttc gagcattggt acgacaacca
     4861 aatgaaatca atcaatgaat ggttgacaga acgtcttcaa caatcgttga gtgcaactca
     4921 atacatttcc ctttcgacaa ttgtaaaaaa agtgtatcaa gacttttcac tgcaaggaat
     4981 cgatgaagaa cgactgaata gtaagactta tcagtcaatc agtcgacgat tacagcttga
     5041 agagtcaaat tctcatattc aagaaggtat acgaaaacat taaataccgg tcagatatca
     5101 ctcgaggagc tcccgagatc ttcagcgctc agcattcgta gaattgtaag tttaaacttt
     5161 ctcatactaa ctaacgcgct ctatttaaat tttcagaatt ccaactgagc gccggtcgct
     5221 accattacca acttgtctgg tgtcaaaaat aataggggcc gctgtcatca gagtaagttt
     5281 aaactgagtt ctactaacta acgagtaata tttaaatttt cagcatctcg cgcccgtgcc
     5341 tctgacttct aagtccaatt actcttcaac atccctacat gctctttctc cctgtgctcc
     5401 caccccctat ttttgttatt atcaaaaaac ttctcttaat ttctttgttt tttagcttct
     5461 tttaagtcac ctctaacaat gaaattgtgt agattcaaaa atagaattaa ttcgtaataa
     5521 aaagtcgaaa aaaattgtgc tccctccccc cattaataat aattctatcc caaaatctac
     5581 acaatgttct gtgtacactt cttatgtttt ttacttctga taaatttttt tgaaacatca
     5641 tagaaaaaac cgcacacaaa ataccttatc atatgttacg tttcagttta tgaccgcaat
     5701 ttttatttct tcgcacgtct gggcctctca tgacgtcaaa tcatgctcat cgtgaaaaag
     5761 ttttggagta tttttggaat ttttcaatca agtgaaagtt tatgaaatta attttcctgc
     5821 ttttgctttt tggggtttcc cctattgttt gtcaagattt cgaggacggc gtttttcttg
     5881 ctaaaatcac aagtattgat gagcacgatg caagaaagat cggaagaagg tttgggtttg
     5941 aggctcagtg gaaggtgagt agaagttgat aatttgaaag tggagtagtg tctatggggt
     6001 ttttgcctta aatgacagaa tacattccca atataccaaa cataactgtt tcctactagt
     6061 cggccgtacg ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     6121 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     6181 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
     6241 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
     6301 agaaaatacc gcatcaggcg gccttaaggg cctcgtgata cgcctatttt tataggttaa
     6361 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     6421 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     6481 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     6541 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     6601 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     6661 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     6721 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     6781 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     6841 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     6901 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     6961 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     7021 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     7081 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     7141 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     7201 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     7261 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     7321 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     7381 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     7441 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     7501 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     7561 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     7621 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     7681 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     7741 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     7801 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     7861 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     7921 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     7981 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     8041 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     8101 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     8161 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     8221 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     8281 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     8341 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     8401 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     8461 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     8521 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
     8581 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
     8641 gatggatacg ctaacaactt ggaaatgaaa taagcttggg ctgcagga
//
