LOCUS       sequence-21250-e         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Fiordalisi JJ, Johnson RL 2nd, Ulku AS, Der CJ, Cox AD
  TITLE     Mammalian expression vectors for Ras family proteins: generation and
            use of expression constructs to analyze Ras family function.
  JOURNAL   Methods Enzymol. 2001;332:3-36. doi: 10.1016/s0076-6879(01)32189-4.
  PUBMED    11305105
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2001"; volume: "332:3-36. doi"; pages: " 
            10.1016/s0076-6879(01)32189-4"
COMMENT     Sequence Label: pBABE H-Ras 61L, 186S
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(603..623)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     gap             815..826
                     /estimated_length=12
ORIGIN
        1 gnnnnngacg ganataagct ggtggtggtg ggcgccggcg gtgtgggcaa gagtgcgctg
       61 accatccagc tgatccagaa ccactttgtg gacgaatacg accccactat agaggattcc
      121 taccggaagc aggtggtcat tgatggggag acgtgcctgt tggacatcct ggataccgcc
      181 ggcctggagg agtacagcgc catgcgggac cagtacatgc gcaccgggga gggcttcctg
      241 tgtgtgtttg ccatcaacaa caccaagtct tttgaggaca tccaccagta cagggagcag
      301 atcaaacggg tgaaggactc ggatgacgtg cccatggtgc tggtggggaa caagtgtgac
      361 ctggctgcac gcactgtgga atctcggcag gctcaggacc tcgcccgaag ctacggcatc
      421 ccctacatcg agacctcggc caagacccgg cagggagtgg aggatgcctt ctacacgttg
      481 gtgcgtgaga tccggcagca caagctgcgg aagctgaacc ctcctgatga gagtggcccc
      541 ggctgcatga gctgcaagag cgtgctctcc tgagaattcg ccagcacagt ggtcgaccct
      601 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
      661 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccnn ntccccagca
      721 ggcagaagta tgcaaagcat gcatctcann antnnnnnan cnntagtccc gcccctaann
      781 nccncccatc ccgncccnna ncnnnnnncn anttnnnnnn nnnnnnc
//
