LOCUS       sequence-212517-        8667 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the C. elegans acy--1 P260S gain-of-function cDNA 
            pan-neuronally.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8667)
  AUTHORS   Schade MA, Reynolds NK, Dollins CM, Miller KG
  TITLE     Mutations that rescue the paralysis of Caenorhabditis elegans ric-8 
            (synembryn) mutants activate the G alpha(s) pathway and define a 
            third major branch of the synaptic signaling network.
  JOURNAL   Genetics. 2005 Feb;169(2):631-49. doi: 10.1534/genetics.104.032334. 
            Epub 2004 Oct 16.
  PUBMED    15489510
REFERENCE   2  (bases 1 to 8667)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.104.032334"; journalName: "Genetics"; date: 
            "2005-02"; volume: "169"; issue: "2"; pages: "631-49"
COMMENT     Sequence Label: KG#83
FEATURES             Location/Qualifiers
     source          1..8667
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(6123..6145)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6245..6264
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6342..6360)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6428..6532
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6533..6601,6602..7393)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6751..6770)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7564..8152
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8053..8072
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8306..8323
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8440..8461
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8476..8481,8482..8499,8500..8506)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8514..8530
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8519..8541
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8538..8554
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8538..8554
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aacttggaaa tgaaataagc ttgggctgca ggatcttcag atgggagcag tggactgtct
       61 atttgatatt cttaaaatgt actctataaa agatgaaact gtaatgacca attccctaca
      121 taaatatttt taactaattg gtcggatatt tggggatcag agaagtactt gtttttgcga
      181 tattaataat gatatatttt tctagtaaaa aaatgttaag cgatttgtga ataattaagt
      241 tggcttgaaa aagatttttt gatagctatg tattttgtta atttaaatca gattttaaaa
      301 ctttattatt gactatttat atatattata tattatatat tttcatggtt taatcaaaat
      361 ctattaaatt atttctaaaa tctctaaata tcgaattctc taaaaagctt catcaacaaa
      421 agcatttcaa ttatctaaca caatgagaat aattcaagaa ctgaaataga aagaaatcta
      481 gaaaaaaaag agagtgaggt gccctattaa atcggtgctc tcctctctaa cagaagcgcc
      541 tacacgcacc caagagagct ctttttcttt cttttttcgg agcacacata cacgcataca
      601 ctcctcagaa gtgtatagag aaaatgggag aagaagcgaa agaagacgaa gaagcggttg
      661 tgatgtatgc atcgggcgtt tctatctctc tccgtgagca acgagctagt caacccatcg
      721 atccattttt gtgagcacac acagagagag actcaaacac actcacagct tcacactgag
      781 ctccaagagc tctgtagaga gaagccagag tatgctttcc tcgattttcc taacgtcttc
      841 ttcttctgct tttttctttt tcccaacgag ttgtagtcca ctaacttcaa tttttcctcc
      901 agagaaagag ttttagagag acagctgata aatacactag agaaataaaa agaatgataa
      961 aaatttgcta ttttaatcag ttccctctcg tttctctaag ttttgttgtt gggttgggaa
     1021 attaaaattg gaaaatggaa ataacgtttt tttctgtcta atcttgaatt ctccagacat
     1081 ttcttatttc cttttttgtt gcaagttttt cttccttctc taatttttaa tcaaagatat
     1141 ctcgctatga actttgaatt tttgatattt tgattctata aatacataaa gtaatctaaa
     1201 tgctctttta aaataaatct acagtagccc tattttcagg gatccccggg attggccaaa
     1261 ggacccaaag gtatgtttcg aatgatacta acataacata gaacattttc aggaggaccc
     1321 ttggctagcg tcgacggtac cggtaaaaat ggacgacgat gtcggtgaac gaacaccggc
     1381 tctcggtggt tcatgtggac catcagttcg tgcacacagt agttccccaa gacgtgttcc
     1441 attatttgag agagcatctg ctagatggtg gaatcctcaa tttcgatctg caacactaga
     1501 agctcaatat tggaagtgta gtttcagtca gttaagagat cgatttcgtt ctggattaat
     1561 atatattgcc gttgtaattg ccgcttggac actttattta gctttattcg atagaacttt
     1621 tattcagcat tggatcgttt cactatgcct ctgtgccatt attttcgcga tgtttgcctt
     1681 cacggcgtgc gccgctcaat accaaaggtt ctacatgcca acctcatttc tatgcacttt
     1741 tttgatatgc cttgtcacgt tgctcatctt ctccgcagag aatcaagctg cgtttatgac
     1801 tccagttgca agtcttgcca caagttttca ggtcgttctc ctgatctaca cagtcattcc
     1861 actgccactc tatttatgta tactcatcgg aataatctac tcaatcctat ttgagattct
     1921 caacaagaac aaaattggtc ttgaagaggc cggatacatt aagcttgtcc ttcacgctgg
     1981 tgtccattta ctcggtgtcc atttgttcat tttaactcaa gtgcgacaga ggaaaacgtt
     2041 tctgaaagtg ggacaatcga tgttggcaag aaaggatcta gagttggaga cacagtttaa
     2101 agatcatatg attcagtctg tgatgtctaa aaaggtagct gatgaattac tgaaagatgc
     2161 atcagaactt cgccgacctt ctgcttccaa cgactcaaac tgccgaactt caaatgctac
     2221 acaagttgac cagccccttg ccaagatggt acctgaatac cgaaagttcc gtccatttac
     2281 gatgaatctc atgacaaatg tgtcgattct gtttgcggat attgctgggt ttactaaaat
     2341 gtcatcaaat aaatctgccg acgaacttgt caatctgctc aatgatcttt ttggaagatt
     2401 cgatactttg tgtcgattga gaggattaga gaagatttca actttgggag attgttacta
     2461 ttgtgttgct ggatgtcctg aaccatgtga tgatcatgca tgcagaacag tcgaaatggg
     2521 tctcgacatg attgttgcaa tccgccaatt tgatattgat cgtggacaag aagtgaatat
     2581 gcgtgtggga attcatacag gaaaagtaat gtgcggaatg gttggaacaa aaagattcaa
     2641 attcgatgtg ttctcaaacg atgtcacact ggcaaatgag atggaatctt ctggtgttgc
     2701 tggaagagtt cacgtttccg aagcaactgc aaaacttttg aaagggcttt atgaaattga
     2761 agagggaccg gattatgatg gtccattgag gatgcaagtt cagggaactg aacgtcgtgt
     2821 taaaccagaa agcatgaaga ctttctttat caaaggacgt attaatgatg gtgtagaaga
     2881 agaagtgatg caagttcagg aagttgagag ccttcacagt cagaaaagca gtaaaaagtc
     2941 gactctaaaa cagaaatggg cggaaaagtt gaaaatgaac cacacaaatt cttatccaat
     3001 gcgagccgct gcacgtgaag gaggtggaag tttgagaata aaattggcag agagaaatag
     3061 aagtacacag ttactgccaa aagaatcaaa ttcaatttgt ataatggaag ataaccgaaa
     3121 gtcagcatca cttcaagcat tggcaacgaa taattttaat ggaagtaata cagatacaaa
     3181 taacacttat agtgaacgtg gagttgctgg aagtgttagt aagaagtctg tagctggaag
     3241 tgaaagtaat agtataaaag gaagtagaag cagtggacta caattatctc tacaagatgg
     3301 aaattctgat ttaaattcag ttggaggttt ggatactgca atcagtcacc atcacaatgc
     3361 agcatctctc actcgatttg atactgataa caattttgat cagcgtttgg caatggtaat
     3421 tggacaagga gaaggaggat ttgataaagg attttggaat catcatgata gtttgaataa
     3481 gtggactctt cgatttaatg aaaaggacgt tgaagaggaa tatcgagctc attttgtgga
     3541 ttcatcggaa agatatacgg catcaaagaa aggtcatgtt gaaagacata aagatttgat
     3601 ggaacaaggt ggagaaaaag atggaattac tggatcaacg gtcaacaagt atagatattc
     3661 aggagtcttc atcgatataa ttgttgccac gttgatattt gtaatatcgg gtgcagttgc
     3721 aattatgtct gtccgtccat tcccattatc actcattgca tacttcccat ttgctgctgc
     3781 aattctcatt cttacaattg tccttattgg tcttccactt ctcgcccgta aaaaatcatt
     3841 ccaatgtgca aatcaatgga tgccacgtca tctcatcggt cttcttctga tctttcttcc
     3901 aatcggtgtc gcaatctgta tcatgccatt gtgccaaagt ggagactgtg caaatgtaat
     3961 tctgaactac cgtcttgcat tcagttatgt caccattctt gccatattcg ctcattgcaa
     4021 cttctcccaa ttagctgcct ggccaaaaac tactgctgca gtatttatcg gacttctcca
     4081 cattgctgga gttttctact gcgaattcaa tttgaaacac ttagttgaag agcaagacac
     4141 ctgcaacgtg acagcaatca tgattccacc aattcgcaaa ggtctgaact acacaattgc
     4201 attgaactcg acatctgcaa gaacattatc tcaagatttt ggatctccat tattcatttg
     4261 ggagctgctt ctggatgtga tcctgtcgat tgttctagtt gcattcctta attatcagtt
     4321 cgaaaccgca ttccgtatgt cattctttgg agatgttcaa gctcgtcggg acactgaaag
     4381 aatgcaaatc gtgcgtgatc aagccgattg gctcctgaat aacgtgatcc cagcccacgc
     4441 agtagaaagt cttaaaacgg ataccaaata cagtgaaaac catgaaacag ttggtgtact
     4501 gttcgcttca atcactaatt ggaatgatat gtatgaagag aatttcgaag ggggcagaga
     4561 attcttgaga gttctcaatg aagttattgg tgactttgat gagcttctcg atcggccgga
     4621 cttcacgcat attgaaaaaa tcaaaacaat tgggcctgcg tatatggcag caagtggatt
     4681 gaatccagaa cgaaaaaaga atatgttgca tccaaaagag catctatatc agatggttga
     4741 ttttgcattg gcagttcaac atgtactcag tgttttcaac gaggatttgc tcaattttga
     4801 ttttgtttgc aagttaggac ttaatattgg acccgtcact gctggtgtca ttggaacaac
     4861 aaagttgtac tacgatatct ggggtgacac tgtaaacatt gctagccgaa tgtacagcac
     4921 cggagtttta aatcgcattc aagtatcaca acacacacga gaatatctcc tggatcggta
     4981 tgaattcgag ttccgcgatc atattgaagt caagggaatt gatggaggaa tggatactta
     5041 tcttttggtt ggtcgaaaag gtgatggaat acctccatca atcaaggata accaagaaga
     5101 cgaattttaa atctcgagga gctcccgaga tcttcagcgc tcagcattcg tagaattgta
     5161 agtttaaact ttctcatact aactaacgcg ctctatttaa attttcagaa ttccaactga
     5221 gcgccggtcg ctaccattac caacttgtct ggtgtcaaaa ataatagggg ccgctgtcat
     5281 cagagtaagt ttaaactgag ttctactaac taacgagtaa tatttaaatt ttcagcatct
     5341 cgcgcccgtg cctctgactt ctaagtccaa ttactcttca acatccctac atgctctttc
     5401 tccctgtgct cccaccccct atttttgtta ttatcaaaaa acttctctta atttctttgt
     5461 tttttagctt cttttaagtc acctctaaca atgaaattgt gtagattcaa aaatagaatt
     5521 aattcgtaat aaaaagtcga aaaaaattgt gctccctccc cccattaata ataattctat
     5581 cccaaaatct acacaatgtt ctgtgtacac ttcttatgtt ttttacttct gataaatttt
     5641 tttgaaacat catagaaaaa accgcacaca aaatacctta tcatatgtta cgtttcagtt
     5701 tatgaccgca atttttattt cttcgcacgt ctgggcctct catgacgtca aatcatgctc
     5761 atcgtgaaaa agttttggag tatttttgga atttttcaat caagtgaaag tttatgaaat
     5821 taattttcct gcttttgctt tttggggttt cccctattgt ttgtcaagat ttcgaggacg
     5881 gcgtttttct tgctaaaatc acaagtattg atgagcacga tgcaagaaag atcggaagaa
     5941 ggtttgggtt tgaggctcag tggaaggtga gtagaagttg ataatttgaa agtggagtag
     6001 tgtctatggg gtttttgcct taaatgacag aatacattcc caatatacca aacataactg
     6061 tttcctacta gtcggccgta cgggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
     6121 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     6181 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
     6241 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
     6301 agatgcgtaa ggagaaaata ccgcatcagg cggccttaag ggcctcgtga tacgcctatt
     6361 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     6421 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     6481 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     6541 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     6601 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     6661 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     6721 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     6781 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     6841 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     6901 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     6961 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     7021 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     7081 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     7141 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     7201 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     7261 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     7321 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     7381 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     7441 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     7501 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     7561 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     7621 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     7681 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     7741 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     7801 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     7861 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     7921 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     7981 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     8041 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     8101 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     8161 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     8221 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     8281 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     8341 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     8401 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     8461 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     8521 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc tgtaagttta
     8581 aacatgatct tactaactaa ctattctcat ttaaattttc agagcttaaa aatggctgaa
     8641 atcactcaca acgatggata cgctaac
//
