LOCUS       sequence-21252-e         671 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Fiordalisi JJ, Johnson RL 2nd, Ulku AS, Der CJ, Cox AD
  TITLE     Mammalian expression vectors for Ras family proteins: generation and
            use of expression constructs to analyze Ras family function.
  JOURNAL   Methods Enzymol. 2001;332:3-36. doi: 10.1016/s0076-6879(01)32189-4.
  PUBMED    11305105
REFERENCE   2  (bases 1 to 671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2001"; volume: "332:3-36. doi"; pages: " 
            10.1016/s0076-6879(01)32189-4"
COMMENT     Sequence Label: pCGN H-Ras 61L, 189L
FEATURES             Location/Qualifiers
     source          1..671
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     5'UTR           95..134
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             164..190
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             170..196
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 agctcgttta gtggaccgtc agaatcgcct ggagacgcca tccacgctgt tttgacctcc
       61 atagaagaca ccgggaccga tccagcctcc gggggatctt ggtggcgtga aactcccgca
      121 cctcttcggc cagcgccttg tagaagcgcg tatggcttct agctatcctt atgacgtgcc
      181 tgactatgcc agcctgggag gaccttctag cggatccacc atgacagaat acaagcttgt
      241 ggtggtgggc gctggaggcg tgggaaagag tgccctgacc atccagctga tccagaacca
      301 ctttgtggac gaatacgacc ccactataga ggattcctac cggaagcagg tggtcattga
      361 tggggagacg tgcctgttgg acatcctgga taccgccggc ctggaggagt acagcgccat
      421 gcgggaccag tacatgcgca ccggggaggg cttcctgtgt gtgtttgcca tcaacaacac
      481 caagtctttt gaggacatcc accagtacag ggagcagatc aaacgggtga aggactcgga
      541 tgacgtgccc atggtgctgg tggggaacaa gtgtgacctg gctgcacgca ctgtggaatc
      601 tcggcaggct caggacctcg cccgaagcta cggcatcccc tacatcgaga cctcggccaa
      661 gacccggcag g
//
