LOCUS       sequence-212521-        6930 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the C. elegans pde-4 (isoform d) cDNA pan-neuronally.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6930)
  AUTHORS   Charlie NK, Thomure AM, Schade MA, Miller KG
  TITLE     The Dunce cAMP phosphodiesterase PDE-4 negatively regulates G 
            alpha(s)-dependent and G alpha(s)-independent cAMP pools in the 
            Caenorhabditis elegans synaptic signaling network.
  JOURNAL   Genetics. 2006 May;173(1):111-30. doi: 10.1534/genetics.105.054007. 
            Epub 2006 Apr 19.
  PUBMED    16624912
REFERENCE   2  (bases 1 to 6930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.105.054007"; journalName: "Genetics"; date: 
            "2006-05"; volume: "173"; issue: "1"; pages: "111-30"
COMMENT     Sequence Label: KG#203
FEATURES             Location/Qualifiers
     source          1..6930
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(4074..4096)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4196..4215
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4293..4311)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4379..4483
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4484..4552,4553..5344)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4702..4721)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5515..6103
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6004..6023
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    6391..6412
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6427..6432,6433..6450,6451..6457)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6465..6481
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6470..6492
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6489..6505
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6489..6505
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ctatttatat atattatata ttatatattt tcatggttta atcaaaatct attaaattat
       61 ttctaaaatc tctaaatatc gaattctcta aaaagcttca tcaacaaaag catttcaatt
      121 atctaacaca atgagaataa ttcaagaact gaaatagaaa gaaatctaga aaaaaaagag
      181 agtgaggtgc cctattaaat cggtgctctc ctctctaaca gaagcgccta cacgcaccca
      241 agagagctct ttttctttct tttttcggag cacacataca cgcatacact cctcagaagt
      301 gtatagagaa aatgggagaa gaagcgaaag aagacgaaga agcggttgtg atgtatgcat
      361 cgggcgtttc tatctctctc cgtgagcaac gagctagtca acccatcgat ccatttttgt
      421 gagcacacac agagagagac tcaaacacac tcacagcttc acactgagct ccaagagctc
      481 tgtagagaga agccagagta tgctttcctc gattttccta acgtcttctt cttctgcttt
      541 tttctttttc ccaacgagtt gtagtccact aacttcaatt tttcctccag agaaagagtt
      601 ttagagagac agctgataaa tacactagag aaataaaaag aatgataaaa atttgctatt
      661 ttaatcagtt ccctctcgtt tctctaagtt ttgttgttgg gttgggaaat taaaattgga
      721 aaatggaaat aacgtttttt tctgtctaat cttgaattct ccagacattt cttatttcct
      781 tttttgttgc aagtttttct tccttctcta atttttaatc aaagatatct cgctatgaac
      841 tttgaatttt tgatattttg attctataaa tacataaagt aatctaaatg ctcttttaaa
      901 ataaatctac agtagcccta ttttcaggga tccccgggat tggccaaagg acccaaaggt
      961 atgtttcgaa tgatactaac ataacataga acattttcag gaggaccctt ggctagcaaa
     1021 aatgccacga agacgcggct cgtcgtcgtc gtcgtcggcg gcaggtggta gtggcggcgg
     1081 cggaggcttc ggattctcga gcctccgacg cgaactccac ttgcacaact tctttcgaac
     1141 ctcttcgccg tcggcctcgt cgacgtcgag aacaccgccc gcagcattac caccgcgcac
     1201 atcagctgtt actattccgg gcagtaatca caaattgacc tcttccgctt catcttacca
     1261 cccaccacgc gagcttaccg tgtcgacgtt cagcgccggc tcggcaactg cagctgatgg
     1321 acttggaggt gctcatctaa cgccatcact ttcatcttcg gtacatgcga gacgggagtc
     1381 gtttctctac cgtgccagtg atgatcttcg agaagcgtct tcgttacgac cagtcagtcg
     1441 ggcgtcgtca atcgcgtcca atgagcacgg acacggtgac gacctgattg taaccccgtt
     1501 tgctcaactg cttgccagtc ttcgaaacgt tcgatccaat ctgatttcga taaccaacat
     1561 ccagaacagc gacgacagtc gacacgctaa tcgaagtgcg aaacggccgc ctttgcacaa
     1621 cattgagctt ccagacgatg tggtgcattg tgcgcatgat acactcgaag aactcgattg
     1681 gtgtcttgat cagctggaaa caattcaaac tcatcgatca gtgtctgaga tggccagcag
     1741 taagttccgc aagatgctca acaaagagtt gtcacacttt gctgaatcgt ccaaatccgg
     1801 cactcaggtc tccaagtttt tgatcaccac ctacatggac aaggaagagg atgaaccgtc
     1861 gatcgagatc gaagtgccca ctgaggtaca aggcccttct acatctggac caatgacact
     1921 ttcgatactg aaaaaagctc aaacagcagc catgaataag atctcaggag ttcgaaaact
     1981 tcgtgctcca tcacatgacg gccatgttcc cgaatacggt gtaaactgtg cacgtgagat
     2041 agctgttcat atgcaacgac tcgatgactg gggacctgat gtcttcaaaa tcgacgaact
     2101 ttccaaaaat cactcgctca ctgtcgttac attttcatta ctccgccagc ggaatctctt
     2161 taaaactttt gaaattcatc aatctactct ggtcacctac ttgctcaatc ttgaacatca
     2221 ctatcgtaat aatcattatc acaattttat tcatgcagcc gatgttgcac agtcaatgca
     2281 cgttctgctc atgtctcccg tccttacaga agtcttcact gatcttgaag tattagcagc
     2341 tatatttgcc ggtgctgtcc atgatgtaga tcatcctgga ttcacgaatc agtatttgat
     2401 caactcaaac aatgaattag cgatcatgta caatgatgaa agtgtccttg agcagcatca
     2461 tctggcagtt gcattcaagc ttttgcaaga ttcaaactgt gatttccttg caaatttgtc
     2521 cagaaaacaa cggctgcaat ttcgcaagat tgtcattgat atggtacttg caactgatat
     2581 gtctaagcac atgtctcttc ttgctgatct gaaaacaatg gtcgaagcaa agaaagttgc
     2641 tggaaacaat gtgattgtgc tggacaagta caatgacaaa attcaggtac tacaatcgat
     2701 gattcatctc gctgatctct caaatccaac taagccaatc gaattatacc aacaatggaa
     2761 tcaaaggata atggaagaat actggaggca gggagataaa gagaaagagc ttggacttga
     2821 gatatcaccg atgtgcgatc gaggaaatgt gaccattgaa aaatcacagg tcggattcat
     2881 cgactacatt gttcatccat tatacgaaac atgggcagat cttgtctatc cagatgctca
     2941 aaatatactc gatcaactcg aagaaaatcg agaatggtac caatcacgaa tacctgaaga
     3001 acctgacaca gctcgtactg taacagaaga tgacgagcac aaataaatac cggtcagata
     3061 tcactcgagg agctcccgag atcttcagcg ctcagcattc gtagaattgt aagtttaaac
     3121 tttctcatac taactaacgc gctctattta aattttcaga attccaactg agcgccggtc
     3181 gctaccatta ccaacttgtc tggtgtcaaa aataataggg gccgctgtca tcagagtaag
     3241 tttaaactga gttctactaa ctaacgagta atatttaaat tttcagcatc tcgcgcccgt
     3301 gcctctgact tctaagtcca attactcttc aacatcccta catgctcttt ctccctgtgc
     3361 tcccaccccc tatttttgtt attatcaaaa aacttctctt aatttctttg ttttttagct
     3421 tcttttaagt cacctctaac aatgaaattg tgtagattca aaaatagaat taattcgtaa
     3481 taaaaagtcg aaaaaaattg tgctccctcc ccccattaat aataattcta tcccaaaatc
     3541 tacacaatgt tctgtgtaca cttcttatgt tttttacttc tgataaattt ttttgaaaca
     3601 tcatagaaaa aaccgcacac aaaatacctt atcatatgtt acgtttcagt ttatgaccgc
     3661 aatttttatt tcttcgcacg tctgggcctc tcatgacgtc aaatcatgct catcgtgaaa
     3721 aagttttgga gtatttttgg aatttttcaa tcaagtgaaa gtttatgaaa ttaattttcc
     3781 tgcttttgct ttttggggtt tcccctattg tttgtcaaga tttcgaggac ggcgtttttc
     3841 ttgctaaaat cacaagtatt gatgagcacg atgcaagaaa gatcggaaga aggtttgggt
     3901 ttgaggctca gtggaaggtg agtagaagtt gataatttga aagtggagta gtgtctatgg
     3961 ggtttttgcc ttaaatgaca gaatacattc ccaatatacc aaacataact gtttcctact
     4021 agtcggccgt acgggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga
     4081 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     4141 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca
     4201 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta
     4261 aggagaaaat accgcatcag gcggccttaa gggcctcgtg atacgcctat ttttataggt
     4321 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     4381 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     4441 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     4501 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     4561 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     4621 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     4681 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     4741 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     4801 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     4861 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     4921 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     4981 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     5041 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     5101 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     5161 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     5221 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     5281 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     5341 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     5401 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     5461 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     5521 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     5581 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     5641 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     5701 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     5761 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     5821 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     5881 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     5941 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     6001 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     6061 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     6121 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     6181 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     6241 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     6301 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     6361 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     6421 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     6481 atttcacaca ggaaacagct atgaccatga ttacgccaag ctgtaagttt aaacatgatc
     6541 ttactaacta actattctca tttaaatttt cagagcttaa aaatggctga aatcactcac
     6601 aacgatggat acgctaacaa cttggaaatg aaataagctt gggctgcagg atcttcagat
     6661 gggagcagtg gactgtctat ttgatattct taaaatgtac tctataaaag atgaaactgt
     6721 aatgaccaat tccctacata aatattttta actaattggt cggatatttg gggatcagag
     6781 aagtacttgt ttttgcgata ttaataatga tatatttttc tagtaaaaaa atgttaagcg
     6841 atttgtgaat aattaagttg gcttgaaaaa gattttttga tagctatgta ttttgttaat
     6901 ttaaatcaga ttttaaaact ttattattga
//
