LOCUS       sequence-212523-        1214 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminally FLAG-tagged human RNMT 121-476 in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Trotman JB, Giltmier AJ, Mukherjee C, Schoenberg DR
  TITLE     RNA guanine-7 methyltransferase catalyzes the methylation of 
            cytoplasmically recapped RNAs.
  JOURNAL   Nucleic Acids Res. 2017 Oct 13;45(18):10726-10739. doi: 
            10.1093/nar/gkx801.
  PUBMED    28981715
REFERENCE   2  (bases 1 to 1214)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2017-10-13- 13"; volume: "45"; issue: 
            "18"; pages: "10726-10739"
COMMENT     Sequence Label: pcDNA3-FLAG-RNMT 121-476
FEATURES             Location/Qualifiers
     source          1..1214
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..30
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             43..54
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 atgggcgact acaaagacga tgacgacaag agcagcggcc atatcgaagg tcgtcatgat
       61 ggcactcaaa ataagagaaa aatagcactt gaggatgttc ctgaaaagca gaaaaatctg
      121 gaagaaggac acagctcaac agtggctgcc cattacaatg aacttcagga agttggtttg
      181 gagaagcgta gtcaaagtcg tattttttac ctaagaaact ttaataattg gatgaaaagt
      241 gttctcattg gagaattttt ggaaaaggta cgacagaaga aaaaacgtga tatcactgtt
      301 ttggacctgg gatgtggtaa aggtggagat ttgctgaaat ggaaaaaagg aagaattaac
      361 aagctagttt gtactgatat tgccgatgtt tctgtcaaac agtgtcagca gcggtatgag
      421 gacatgaaaa atcgtcgtga tagtgaatat attttcagtg cagaatttat aactgctgac
      481 agctcaaagg aacttctgat tgacaaattt cgtgacccac aaatgtgttt tgacatctgc
      541 agttgtcagt ttgtctgtca ttactcattt gagtcttatg agcaggctga catgatgctg
      601 agaaatgcgt gtgagagact tagccctggg ggctatttta ttggtactac tcccaatagc
      661 tttgaattga taagacgcct tgaagcttca gaaacagaat catttggaaa tgaaatatat
      721 actgtgaaat ttcagaagaa aggagattat cctttatttg gctgcaaata tgacttcaac
      781 ttggaaggtg ttgtggatgt tcctgaattc ttggtctatt ttccattgct aaatgaaatg
      841 gcaaagaagt acaatatgaa actagtctac aaaaaaacat ttctggaatt ctacgaagaa
      901 aagattaaga acaatgaaaa taaaatgctc ttaaaacgaa tgcaggcctt ggagccatat
      961 cctgcaaatg agagttctaa acttgtctct gagaaggtgg atgactatga acatgcagca
     1021 aagtacatga agaacagtca agtaaggtta cctttgggaa ccttaagtaa atcagaatgg
     1081 gaagctacaa gtatttactt ggtgtttgcc tttgagaaac agcagtgatg acgagctcgg
     1141 atccactagt aacggccgcc agtgtgctgg aattctgcag atatccatca cactggcggc
     1201 cgctcgagca tgca
//
