LOCUS       sequence-212525-        6937 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses proteins in C. elegans cholinergic neurons in head, 
            ventral nerve cord, and tail.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6937)
  AUTHORS   Williams SL, Lutz S, Charlie NK, Vettel C, Ailion M, Coco C, Tesmer 
            JJ, Jorgensen EM, Wieland T, Miller KG
  TITLE     Trio's Rho-specific GEF domain is the missing Galpha q effector in 
            C. elegans.
  JOURNAL   Genes Dev. 2007 Nov 1;21(21):2731-46. doi: 10.1101/gad.1592007. Epub
            2007 Oct 17.
  PUBMED    17942708
REFERENCE   2  (bases 1 to 6937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.1592007"; journalName: "Genes Dev"; date: "2007-11-1- 
            1"; volume: "21"; issue: "21"; pages: "2731-46"
COMMENT     Sequence Label: KG#94
FEATURES             Location/Qualifiers
     source          1..6937
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(4377..4399)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4499..4518
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4596..4614)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4682..4786
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4787..4855,4856..5647)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5005..5024)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5818..6406
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6307..6326
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6560..6577
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6694..6715
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6730..6735,6736..6753,6754..6760)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6768..6784
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6773..6795
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6792..6808
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6792..6808
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gctcccggcc gccatggccg cgggattaca ccaatcattt ctcccctccg
       61 aaatgtgtag tcgtcatgac aaagtggtga cactggatta cggtaggctc cgcccacttc
      121 ttggaaaagt gacgtgggct cggtgagccc taaagctttt ggttttcaca attttctggt
      181 ttttgggatt tttgcgggaa ttttgagagg agaaagttgg gagagaagat attggaatgg
      241 tccccgggga gctgtacatt tatacatcta gactgaagct ggggtattgc aaaaagagaa
      301 aagcctatca ctttctttcc atcctgcata cttgccaaaa agttttggct gagcttattt
      361 ttaaaattgg aattttttgg aattttcaga aaagaaaaag ttttttcttt ttttttaatt
      421 taaaaaattt ttgctattta ttgcatggta ctacagaaaa acttgaatga aataaaaaat
      481 ttggaaaatt caagaaaact ttttttttca tagtttattt attcgagttt taatgtggct
      541 tttattttgt tcggtttttg ttttaaaaag attttataca aaatttcaag aatttacaat
      601 ttttccgttg taaaaaatcc aaaaaaaaat ccaaaaaaat agttcagacc tttttttaaa
      661 ttttaaattt aaaaattcaa taccaaaaaa ttaaaaaaaa ttagtttttc cgagaataaa
      721 ggaaaaatgt tgaaaaaata ttatatataa aatttccaga aaatgtgaaa actccaaaaa
      781 actgcagact tttccccaaa ctagctattt tttgaattca ttacctttaa aggtggagta
      841 ccgaaattta agactttgct tttttagacc caaaatggtc caaaactacc aaatttcgta
      901 atgagacgtt ctgaaaattt ttcaaaaaaa tgttatggga gctcaaagtt tttgaaaaaa
      961 tggtatattt ttagctaatt ttaaccactt cttggcgtcg cagcggttgg aactcaattt
     1021 taattaaatt atcatccttc aatgtccttc aatctttgca atttcaatcg accatacgcg
     1081 ttcagagcat tttttttttt cgtttttcct cccgtccagc acatatttaa atattctctc
     1141 tccccctccc ctggttccta aaaacttggg aaaggggaat ttcgaaggag gtggaagctt
     1201 cttcttctcc ccgagcacac acacacatcc tctcttcaaa atctttaaga aatagtgcgt
     1261 gtgaatgact agacgctgaa gctaacttgg tttccgcgaa caatcccaat cagtgcctgt
     1321 gccgaggctt cggagaagaa gagattgacg tcgtcttttt ctttttttgt gaaacagaat
     1381 aaaaaagaat acgaaatgga ctttttcaca cattttcgtt acttttccag aaaaaaacga
     1441 agctctagcc atggaagttt gaattgaaaa cctaaacccc gatgtgtatt catttgagct
     1501 taagcctaag cctaattcta cgcctaagca taagcctaag cctatgttca agcctaagcc
     1561 aaattgtaga acgttaccaa aagatcaaac ctatttttcc tacagaaaag gtctaatgat
     1621 aactgttaag cgagagacaa ggagagatat atatcaaaaa ctagcccaaa ctgtgattaa
     1681 catctattaa ctgacctcaa aaaaaaaaat agtagagatt aaatactgct caaaaagttt
     1741 cttggaaaag taaattgtta ggtttctggg gcctgtaaaa ctggaccgag ttttgaagaa
     1801 tttttttaac ttttcaaagt ttggcgtcac tgaaagcttt atattgtaca tgttccacca
     1861 attttctttt gtatccgttt tttctgtctc aaaacggagc tcgagcccat gacgtgtgga
     1921 taccgtgaga atccgcccaa ttccttcaca tcgatctcat atttctttcc cagtttatat
     1981 tgaattcccg ttgaattgcg gctttgcagg ggagaagatg tgcgtcttta tatttttttt
     2041 tctatcctta cccgccgtga atccggctcg aattgagcca atttttatga atagtagagc
     2101 taattgactt cgtctcccta gaattgatga ggtaaagctc gtggggagat atggagattt
     2161 ttcgattttt tttccagtaa ttttcagttc aaaatttgag cttttgagca ggagttttga
     2221 gatttgtgcc aaaatttcct attatttttt caaatatttt tgcttttgta aaaaaggtgt
     2281 agtagaagtt ttgtagtgcc actatttttt ttttgaaaat tatgttttgg cggaaagttc
     2341 tttaaaaaag ttaatcaaaa tttttggcgc tttagtcaaa ttaattttct ggcaaaaata
     2401 ttagtaattt tggcgtgagt tcaattttcc cattcttaaa atttcacaca attaagaatt
     2461 ttaagatttg ggtttttttt tcaacaaaaa atcaaatatt tacatgaaaa ttcggcggga
     2521 aataaaaaaa aatattccga ttcaatgtgt gaataacttt ttgcggggaa ttcaaatttg
     2581 cattatttaa acttttttta agctcgaaaa attgaaattc ttaaataaat aaataaaatt
     2641 ttcctcgaat tttgaatgtt tcgatttttt agcgttattt tttcaaaatt tccactaaaa
     2701 ctctagttct aaagttctaa aactttaaaa acttcctaat gttgtggtct tgtgtttttt
     2761 tggcagcgat ttccaaattt ctgaaattcc aacaaaaaaa ccactacgtc agtttgcata
     2821 tccaaccccc cccccccccc ccccccctct gagccaatca atcatgtgca ttttgaattg
     2881 gcgccaaggc aatcttcgta aaaaataaca atagagtcat ccctcagaac agaaaagaaa
     2941 cccgcccggg ggctcaaata cacatgtcat ttttcttttt tcgcctgatt tttagcctaa
     3001 attagtgttt agcaattttt taaggtaact cggtggagtc atttgaactt tttaaactaa
     3061 aatttcagaa aaaattcttt ttttttgtca aaaaataatt ttcccgccgg aatttttcaa
     3121 aaaaataatt tttattttat ttttcaagtt ccttgtttcc tctttatttt tttattttgc
     3181 ccccaaaagt tttacactaa aatatttaat tttcaggtaa ttcatcgaaa gtctttctat
     3241 tttccgcatc tcttgttcaa gggattggcc aaaggaccca aaggtatgtt tcgaatgata
     3301 ctaacataac atagaacatt ttcaggagga cccttggcta gcgtcgacgg taccggtcag
     3361 atatcactcg aggagctccc gagatcttca gcgctcagca ttcgtagaat tgtaagttta
     3421 aactttctca tactaactaa cgcgctctat ttaaattttc agaattccaa ctgagcgccg
     3481 gtcgctacca ttaccaactt gtctggtgtc aaaaataata ggggccgctg tcatcagagt
     3541 aagtttaaac tgagttctac taactaacga gtaatattta aattttcagc atctcgcgcc
     3601 cgtgcctctg acttctaagt ccaattactc ttcaacatcc ctacatgctc tttctccctg
     3661 tgctcccacc ccctattttt gttattatca aaaaacttct cttaatttct ttgtttttta
     3721 gcttctttta agtcacctct aacaatgaaa ttgtgtagat tcaaaaatag aattaattcg
     3781 taataaaaag tcgaaaaaaa ttgtgctccc tccccccatt aataataatt ctatcccaaa
     3841 atctacacaa tgttctgtgt acacttctta tgttttttac ttctgataaa tttttttgaa
     3901 acatcataga aaaaaccgca cacaaaatac cttatcatat gttacgtttc agtttatgac
     3961 cgcaattttt atttcttcgc acgtctgggc ctctcatgac gtcaaatcat gctcatcgtg
     4021 aaaaagtttt ggagtatttt tggaattttt caatcaagtg aaagtttatg aaattaattt
     4081 tcctgctttt gctttttggg gtttccccta ttgtttgtca agatttcgag gacggcgttt
     4141 ttcttgctaa aatcacaagt attgatgagc acgatgcaag aaagatcgga agaaggtttg
     4201 ggtttgaggc tcagtggaag gtgagtagaa gttgataatt tgaaagtgga gtagtgtcta
     4261 tggggttttt gccttaaatg acagaataca ttcccaatat accaaacata actgtttcct
     4321 actagtcggc cgtacgggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
     4381 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     4441 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
     4501 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
     4561 gtaaggagaa aataccgcat caggcggcct taagggcctc gtgatacgcc tatttttata
     4621 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     4681 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     4741 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     4801 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     4861 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     4921 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     4981 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     5041 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     5101 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     5161 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     5221 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     5281 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     5341 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     5401 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     5461 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     5521 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     5581 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     5641 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     5701 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     5761 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     5821 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     5881 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     5941 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     6001 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     6061 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     6121 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     6181 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     6241 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     6301 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     6361 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     6421 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     6481 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     6541 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     6601 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     6661 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     6721 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     6781 acaatttcac acaggaaaca gctatgacca tgattacgcc aagctgtaag tttaaacatg
     6841 atcttactaa ctaactattc tcatttaaat tttcagagct taaaaatggc tgaaatcact
     6901 cacaacgatg gatacgctaa caacttggaa atgaaat
//
