LOCUS       sequence-21253-e         604 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Fiordalisi JJ, Johnson RL 2nd, Ulku AS, Der CJ, Cox AD
  TITLE     Mammalian expression vectors for Ras family proteins: generation and
            use of expression constructs to analyze Ras family function.
  JOURNAL   Methods Enzymol. 2001;332:3-36. doi: 10.1016/s0076-6879(01)32189-4.
  PUBMED    11305105
REFERENCE   2  (bases 1 to 604)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2001"; volume: "332:3-36. doi"; pages: " 
            10.1016/s0076-6879(01)32189-4"
COMMENT     Sequence Label: pCGN K-Ras 12V, 188L
FEATURES             Location/Qualifiers
     source          1..604
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     5'UTR           84..123
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             153..179
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             159..185
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
       61 cgggaccgat ccagcctccg ggggatcttg gtggcgtgaa actcccgcac ctcttcggcc
      121 agcgccttgt agaagcgcgt atggcttcta gctatcctta tgacgtgcct gactatgcca
      181 gcctgggagg accttctagc ggatccacca tgactgaata taaacttgtg gtagttggag
      241 ctgttggcgt aggcaagagt gccttgacga tacagctaat tcagaatcat tttgtggacg
      301 aatatgatcc aacaatagag gattcctaca ggaagcaagt agtaattgat ggagaaacct
      361 gtctcttgga tattctcgac acagcaggtc aagaggagta cagtgcaatg agggaccagt
      421 acatgaggac tggggagggc tttctttgtg tatttgccat aaataatact aaatcatttg
      481 aagatattca ccattataga gaacaaatta aaagagttaa ggactctgaa gatgtaccta
      541 tggtcctagt aggaaataaa tgtgatttgc cttccagaac agtagacaca aaacaggctc
      601 agga
//
