LOCUS       sequence-212537-        2543 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Upstream homology arm, RecJ is a ssDNA specifiC exonuclease, 
            involved in genome repair and maintenance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Wicke N, Radford D, Verrone V, Wipat A, French CE
  TITLE     BacilloFlex: A modular DNA assembly toolkit for Bacillus subtilis 
            synthetic biology
  JOURNAL   bioRxiv 185108
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            185108"
COMMENT     Sequence Label: pTU1-A-recJU
FEATURES             Location/Qualifiers
     source          1..2543
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(491..508)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(662..1250)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(742..761)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1421..2212,2213..2281))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2044..2063
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2282..2386)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2454..2472
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2476..2519)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2522..2541
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
ORIGIN
        1 cgtctcaatc tgggtgactt cctgatgaac tgcttgacct ggcggctatc ggcaccattg
       61 ccgatctagt cccgctgcat gacgaaaaca gattaatagc aacactagga cttgaaaggc
      121 ttcgacggac aaatcggctc ggcttaaaag agctgattaa gctgtcaggc ggagacatcg
      181 gagaagcgaa cgaagaaaca gtcggatttc agctcgcacc aaggctgaat gccgtcggaa
      241 gaatcgaaca ggcggatccc gctgttcatt tgcttatgtc agaggattcg tttgaagcgg
      301 aagagcttgc cgcagaaatt gaccagctga acaaagagcg ccaaaaaatg gtcagcaaaa
      361 tgacggatga agcgattgaa atggttgaac agcaagggct cgatcaaact gcaattgtcg
      421 tggccaaagc cggatggaac ccgggagtcg tcgggattgt ggcttcaatg ccagagacgc
      481 tgcaggcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
      541 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      601 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      661 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      721 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      781 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      841 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      901 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      961 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     1021 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     1081 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     1141 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     1201 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     1261 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     1321 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     1381 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     1441 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1501 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1561 gagagccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1621 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1681 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     1741 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1801 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1861 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1921 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1981 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     2041 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     2101 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     2161 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     2221 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     2281 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     2341 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     2401 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     2461 gtatcacgag gcagaatttc agataaaaaa aatccttagc tttcgctaag gatgatttct
     2521 ggaattcgcg gccgcttcta gaa
//
