LOCUS       sequence-21254-e         649 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Fiordalisi JJ, Johnson RL 2nd, Ulku AS, Der CJ, Cox AD
  TITLE     Mammalian expression vectors for Ras family proteins: generation and
            use of expression constructs to analyze Ras family function.
  JOURNAL   Methods Enzymol. 2001;332:3-36. doi: 10.1016/s0076-6879(01)32189-4.
  PUBMED    11305105
REFERENCE   2  (bases 1 to 649)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2001"; volume: "332:3-36. doi"; pages: " 
            10.1016/s0076-6879(01)32189-4"
COMMENT     Sequence Label: pCGN N-Ras wt
FEATURES             Location/Qualifiers
     source          1..649
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     5'UTR           99..138
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             168..194
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             174..200
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 agcagagctc gtttagtgga ccgtcagatc gcctggagac gccatccacg ctgttttgac
       61 ctccatagaa gacaccggga ccgatccagc ctccggggga tcttggtggc gtgaaactcc
      121 cgcacctctt cggccagcgc cttgtagaag cgcgtatggc ttctagctat ccttatgacg
      181 tgcctgacta tgccagcctg ggaggacctt ctagcggatc caccatgact gagtacaaac
      241 tggtggtggt tggagcaggt ggtgttggga aaagcgcact gacaatccag ctaatccaga
      301 accactttgt agatgaatat gatcccacca tagaggattc ttacagaaaa caagtggtta
      361 tagatggtga aacctgtttg ttggacatac tggatacagc tggacaagaa gagtacagtg
      421 ccatgagaga ccaatacatg aggacaggcg aaggcttcct ctgtgtattt gccatcaata
      481 atagcaagtc atttgcggat attaacctct acagggagca gattaagcga gtaaaagact
      541 cggatgatgt acctatggtg ctagtgggaa acaagtgtga tttgccaaca aggacagttg
      601 atacaaaaca agcccacgaa ctggccaaga gttacgggat tccattcat
//
