LOCUS       sequence-212542-        2609 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Upstream homology arm, rrnO-23S (23S rRNA).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Wicke N, Radford D, Verrone V, Wipat A, French CE
  TITLE     BacilloFlex: A modular DNA assembly toolkit for Bacillus subtilis 
            synthetic biology
  JOURNAL   bioRxiv 185108
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            185108"
COMMENT     Sequence Label: pTU1-A-rrnOU
FEATURES             Location/Qualifiers
     source          1..2609
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(557..574)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(728..1316)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(808..827)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1487..2278,2279..2347))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2110..2129
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2348..2452)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2520..2538
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2542..2585)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2588..2607
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
ORIGIN
        1 cgtctcaatc tatccgggag gaccatcgtc ccaaggctaa atactcccta ggtgaccgat
       61 gagtgaacca gtaccgtgag ggaaaggtga aaagcacccc ggaaggggag tgaaagagat
      121 cctgaaaccg tgtgcctaca agtagtcaga gcccgttaac gggtgatggc gtgccttttg
      181 tagaatgaac cggcgagtta cgatcccgtg caaggttaag cagaagatgc ggagccgcag
      241 cgaaagcgag tctgaatagg gcgcatgagt acgtggtcgt agacccgaaa ccaggtgatc
      301 tacccatgtc cagggtgaag ttcaggtaac actgaatgga ggcccgaacc cacgcacgtt
      361 gaaaagtgcg gggatgaggt gtgggtaggg gtgaaatgcc aatcgaacct ggagatagct
      421 ggttctctcc gaaatagctt tagggctagc ctcaaggtaa gagtcttgga ggtagagcac
      481 tgattggact aggggcccct accgggttac cgaattcagt caaactccga atgctgccag
      541 agacgctgca ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
      601 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
      661 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
      721 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
      781 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
      841 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
      901 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
      961 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     1021 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     1081 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     1141 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     1201 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     1261 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     1321 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     1381 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     1441 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     1501 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     1561 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     1621 taccgcgaga gccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     1681 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     1741 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     1801 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     1861 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     1921 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     1981 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     2041 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     2101 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     2161 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     2221 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     2281 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     2341 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     2401 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     2461 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     2521 ataggcgtat cacgaggcag aatttcagat aaaaaaaatc cttagctttc gctaaggatg
     2581 atttctggaa ttcgcggccg cttctagaa
//
