LOCUS       sequence-212543-        2613 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Upstream homology arm, SpsC is involved in spore coat polysaccharide
            synthesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Wicke N, Radford D, Verrone V, Wipat A, French CE
  TITLE     BacilloFlex: A modular DNA assembly toolkit for Bacillus subtilis 
            synthetic biology
  JOURNAL   bioRxiv 185108
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            185108"
COMMENT     Sequence Label: pTU1-A-spsCU
FEATURES             Location/Qualifiers
     source          1..2613
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(561..578)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(732..1320)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(812..831)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1491..2282,2283..2351))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2114..2133
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2352..2456)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2524..2542
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2546..2589)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2592..2611
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
ORIGIN
        1 cgtctcaatc tccttactca ttgccgttaa tcggtaagga agaaattcag ggaggttaca
       61 gaaacgcttg aatcgggatg gctctctaaa ggcccgaagg ttcagcagtt tgaaaaagag
      121 tttgccgcgt ttgtcggggc gaagcacgcc gtggcggtga attcatgtac cgcagcttta
      181 tttttggctt tgaaagcaaa agggattggg cccggggacg aggtcattac ctctccgctt
      241 acgttcagct cgaccgccaa tacgattatt catacgggag cgacgcctgt gtttgcggat
      301 attgacgaaa acacgctcaa tattgacccg gtaaagcttg aggcggccgt cacaccgcga
      361 acgaaagcag ttgtgcccgt ccactttggc gggcagtcct gcgatatgga tgcaatattg
      421 gctgttgccc aaaatcatgg tttattcgtg ctggaggatg cggcacatgc ggtatatacg
      481 acttataagc aaagaatgat cggttctatc ggggatgcaa cggcattcag cttttacgtg
      541 ccagagacgc tgcaggcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
      601 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
      661 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
      721 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
      781 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
      841 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
      901 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
      961 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     1021 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     1081 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     1141 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     1201 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     1261 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     1321 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     1381 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     1441 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     1501 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     1561 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     1621 atgataccgc gagagccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     1681 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     1741 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     1801 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     1861 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     1921 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     1981 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     2041 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     2101 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     2161 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     2221 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     2281 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     2341 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     2401 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     2461 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     2521 aaaaataggc gtatcacgag gcagaatttc agataaaaaa aatccttagc tttcgctaag
     2581 gatgatttct ggaattcgcg gccgcttcta gaa
//
